pycom12401g00020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012401
OG0029412
Physical Location & Seq
Reverse (-)
15104 .. 15379
276 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12401g00020.1

Sequence Viewer

Length: 276 bp
ATGGAATCTCAAATCTCTCTCGACGCCGAGAATTTTTTATGTGTCCAGGTCGATCAGAGGTTCGGCTTGCTTCTCCCCTACCCTTTAGCGTTCTGGGCCCCTGAAAAAATAAAGAAACCCGAAAAAAAAAGAAAGAAGAGAAATTGGGCCATGTTTTCCGGGAGAGGCCGCCTTCTCTCAGGCCAGCAGTCTTCTACTTCTAGTCGCCTGCTCAATGACGGGCTTCCCAGTTTCCTGGGCTCTACTCGCTCGTCTACGCTCCGACCCAGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

92

Amino Acids

10.31

Weight (kDa)

10.49

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029412)

Species Orthologous Gene IDs
pyrus_communis pycom12401g00020 pycom461g00370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 254
AciI CCGC 1 cut(s) 169
AcsI RAATTY 1 cut(s) 31
AcyI GRCGYC 1 cut(s) 24
AjnI CCWGG 2 cut(s) 45, 234
AoxI GGCC 4 cut(s) 96, 147, 166, 181
ApaI GGGCCC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 31
AspS9I GGNCC 3 cut(s) 96, 97, 147
AsuC2I CCSGG 1 cut(s) 160
BaeGI GKGCMC 1 cut(s) 100
BanII GRGCYC 2 cut(s) 100, 242
BbsI GAAGAC 1 cut(s) 183
BciT130I CCWGG 2 cut(s) 47, 236
BcnI CCSGG 1 cut(s) 160
BfaI CTAG 1 cut(s) 201
BisI GCNGC 1 cut(s) 169
BlsI GCNGC 1 cut(s) 170
Bme1390I CCNGG 3 cut(s) 47, 160, 236
BmgT120I GGNCC 3 cut(s) 96, 97, 147
BmiI GGNNCC 2 cut(s) 98, 99
BmrFI CCNGG 3 cut(s) 47, 160, 236
BmrI ACTGGG 1 cut(s) 222
BmuI ACTGGG 1 cut(s) 222
BpiI GAAGAC 1 cut(s) 183
BpuMI CCSGG 1 cut(s) 160
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 228
BseBI CCWGG 2 cut(s) 47, 236
BseDI CCNNGG 1 cut(s) 235
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 1 cut(s) 228
BseSI GKGCMC 1 cut(s) 100
BseYI CCCAGC 1 cut(s) 266
BshFI GGCC 4 cut(s) 98, 149, 168, 183
BsiSI CCGG 1 cut(s) 159
BsnI GGCC 4 cut(s) 98, 149, 168, 183
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 2 cut(s) 100, 242
Bsp143I GATC 1 cut(s) 52
BspACI CCGC 1 cut(s) 169
BspANI GGCC 4 cut(s) 98, 149, 168, 183
BspCNI CTCAG 1 cut(s) 191
BspLI GGNNCC 2 cut(s) 98, 99
BsrI ACTGG 1 cut(s) 228
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 1 cut(s) 52
BssNI GRCGYC 1 cut(s) 24
Bst2UI CCWGG 2 cut(s) 47, 236
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 24
BstC8I GCNNGC 3 cut(s) 68, 185, 209
BstDEI CTNAG 1 cut(s) 178
BstKTI GATC 1 cut(s) 55
BstMBI GATC 1 cut(s) 52
BstMWI GCNNNNNNNGC 2 cut(s) 95, 246
BstNI CCWGG 2 cut(s) 47, 236
BstSCI CCNGG 3 cut(s) 45, 158, 234
BstSLI GKGCMC 1 cut(s) 100
BstV2I GAAGAC 1 cut(s) 183
BstXI CCANNNNNNTGG 1 cut(s) 235
BsuRI GGCC 4 cut(s) 98, 149, 168, 183
Cac8I GCNNGC 3 cut(s) 68, 185, 209
Cfr13I GGNCC 3 cut(s) 96, 97, 147
CseI GACGC 1 cut(s) 32
CviAII CATG 1 cut(s) 151
CviJI RGCY 7 cut(s) 66, 98, 149, 168, 183, 223, 240
CviKI_1 RGCY 7 cut(s) 66, 98, 149, 168, 183, 223, 240
DdeI CTNAG 1 cut(s) 178
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco24I GRGCYC 2 cut(s) 100, 242
EcoO109I RGGNCCY 1 cut(s) 97
EcoRII CCWGG 2 cut(s) 45, 234
EcoT38I GRGCYC 2 cut(s) 100, 242
FaeI CATG 1 cut(s) 154
FaiI YATR 2 cut(s) 40, 152
FatI CATG 1 cut(s) 150
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 1 cut(s) 169
FriOI GRGCYC 2 cut(s) 100, 242
Fsp4HI GCNGC 1 cut(s) 169
FspBI CTAG 1 cut(s) 201
GluI GCNGC 1 cut(s) 169
GsaI CCCAGC 1 cut(s) 270
HaeIII GGCC 4 cut(s) 98, 149, 168, 183
HapII CCGG 1 cut(s) 159
HgaI GACGC 1 cut(s) 32
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 1 cut(s) 154
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 255
Hpy188I TCNGA 2 cut(s) 57, 263
Hpy188III TCNNGA 1 cut(s) 20
Hpy8I GTNNAC 1 cut(s) 255
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 246
HpyF3I CTNAG 1 cut(s) 178
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 1 cut(s) 154
Kzo9I GATC 1 cut(s) 52
LmnI GCTCC 1 cut(s) 264
MaeI CTAG 1 cut(s) 201
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 2 cut(s) 148, 183
MhlI GDGCHC 2 cut(s) 100, 242
MluCI AATT 2 cut(s) 31, 142
MnlI CCTC 2 cut(s) 51, 158
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 3 cut(s) 47, 160, 236
MvaI CCWGG 2 cut(s) 47, 236
MwoI GCNNNNNNNGC 2 cut(s) 95, 246
NciI CCSGG 1 cut(s) 160
NdeII GATC 1 cut(s) 52
NlaIII CATG 1 cut(s) 154
NlaIV GGNNCC 2 cut(s) 98, 99
NmeAIII GCCGAG 1 cut(s) 52
PfeI GAWTC 1 cut(s) 5
PfoI TCCNGGA 1 cut(s) 158
PkrI GCNGC 1 cut(s) 170
Psp6I CCWGG 2 cut(s) 45, 234
PspFI CCCAGC 1 cut(s) 266
PspGI CCWGG 2 cut(s) 45, 234
PspN4I GGNNCC 2 cut(s) 98, 99
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 3 cut(s) 96, 97, 147
SatI GCNGC 1 cut(s) 169
Sau3AI GATC 1 cut(s) 52
Sau96I GGNCC 3 cut(s) 96, 97, 147
ScrFI CCNGG 3 cut(s) 47, 160, 236
SduI GDGCHC 2 cut(s) 100, 242
SetI ASST 2 cut(s) 51, 62
Sse9I AATT 2 cut(s) 31, 142
SsiI CCGC 1 cut(s) 169
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 3 cut(s) 45, 158, 234
TaqI TCGA 2 cut(s) 21, 51
TasI AATT 2 cut(s) 31, 142
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 1 cut(s) 5
XapI RAATTY 1 cut(s) 31
XmiI GTMKAC 1 cut(s) 254
XspI CTAG 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.