pycom461g00370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0029412
Physical Location & Seq
Forward (+)
294387 .. 294668
282 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom461g00370.1

Sequence Viewer

Length: 282 bp
ATGGAATCTCAAATCTCTCTCGACGCCGAGAATTTTTTATGTGTCCAGGTCGATCAGAGGTTCGGCTTGCTTCTCCCCTACCCTTTAGCGTTCTGGGCCCCTGAAAAAATAAAGAAACCCGAAAAAAAAGAAAGAAGAGAAATTGGGCCATGTTTTCCGGGAGAGGCCGCCTTCTCTCAGGCCAGCAGTCTTCTACTTCTAGTCGCCTGCTCAATGACGGGCTTCCCAGTTTCCTGGGCTCTACTCGCTCGTCTACGCTCCGACCCAGCAAGTAATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

94

Amino Acids

10.34

Weight (kDa)

6.21

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029412)

Species Orthologous Gene IDs
pyrus_communis pycom12401g00020 pycom461g00370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 253
AciI CCGC 1 cut(s) 168
AcsI RAATTY 1 cut(s) 31
AcyI GRCGYC 1 cut(s) 24
AjnI CCWGG 2 cut(s) 45, 233
AoxI GGCC 4 cut(s) 96, 146, 165, 180
ApaI GGGCCC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 31
AspS9I GGNCC 3 cut(s) 96, 97, 146
AsuC2I CCSGG 1 cut(s) 159
BaeGI GKGCMC 1 cut(s) 100
BanII GRGCYC 2 cut(s) 100, 241
BbsI GAAGAC 1 cut(s) 182
BciT130I CCWGG 2 cut(s) 47, 235
BcnI CCSGG 1 cut(s) 159
BfaI CTAG 1 cut(s) 200
BisI GCNGC 1 cut(s) 168
BlsI GCNGC 1 cut(s) 169
Bme1390I CCNGG 3 cut(s) 47, 159, 235
BmgT120I GGNCC 3 cut(s) 96, 97, 146
BmiI GGNNCC 2 cut(s) 98, 99
BmrFI CCNGG 3 cut(s) 47, 159, 235
BmrI ACTGGG 1 cut(s) 221
BmuI ACTGGG 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 234
Bse1I ACTGG 1 cut(s) 227
BseBI CCWGG 2 cut(s) 47, 235
BseDI CCNNGG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 1 cut(s) 227
BseSI GKGCMC 1 cut(s) 100
BseYI CCCAGC 1 cut(s) 265
BshFI GGCC 4 cut(s) 98, 148, 167, 182
BsiSI CCGG 1 cut(s) 158
BsnI GGCC 4 cut(s) 98, 148, 167, 182
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 2 cut(s) 100, 241
Bsp143I GATC 1 cut(s) 52
BspACI CCGC 1 cut(s) 168
BspANI GGCC 4 cut(s) 98, 148, 167, 182
BspCNI CTCAG 1 cut(s) 190
BspLI GGNNCC 2 cut(s) 98, 99
BsrI ACTGG 1 cut(s) 227
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 1 cut(s) 52
BssNI GRCGYC 1 cut(s) 24
Bst2UI CCWGG 2 cut(s) 47, 235
Bst6I CTCTTC 1 cut(s) 130
BstACI GRCGYC 1 cut(s) 24
BstC8I GCNNGC 3 cut(s) 68, 184, 208
BstDEI CTNAG 1 cut(s) 177
BstKTI GATC 1 cut(s) 55
BstMBI GATC 1 cut(s) 52
BstMWI GCNNNNNNNGC 2 cut(s) 95, 245
BstNI CCWGG 2 cut(s) 47, 235
BstSCI CCNGG 3 cut(s) 45, 157, 233
BstSLI GKGCMC 1 cut(s) 100
BstV2I GAAGAC 1 cut(s) 182
BstXI CCANNNNNNTGG 1 cut(s) 234
BsuRI GGCC 4 cut(s) 98, 148, 167, 182
Cac8I GCNNGC 3 cut(s) 68, 184, 208
Cfr13I GGNCC 3 cut(s) 96, 97, 146
CseI GACGC 1 cut(s) 32
CviAII CATG 1 cut(s) 150
CviJI RGCY 7 cut(s) 66, 98, 148, 167, 182, 222, 239
CviKI_1 RGCY 7 cut(s) 66, 98, 148, 167, 182, 222, 239
DdeI CTNAG 1 cut(s) 177
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
Eco24I GRGCYC 2 cut(s) 100, 241
EcoO109I RGGNCCY 1 cut(s) 97
EcoRII CCWGG 2 cut(s) 45, 233
EcoT38I GRGCYC 2 cut(s) 100, 241
FaeI CATG 1 cut(s) 153
FaiI YATR 2 cut(s) 40, 151
FatI CATG 1 cut(s) 149
FblI GTMKAC 1 cut(s) 253
Fnu4HI GCNGC 1 cut(s) 168
FriOI GRGCYC 2 cut(s) 100, 241
Fsp4HI GCNGC 1 cut(s) 168
FspBI CTAG 1 cut(s) 200
GluI GCNGC 1 cut(s) 168
GsaI CCCAGC 1 cut(s) 269
HaeIII GGCC 4 cut(s) 98, 148, 167, 182
HapII CCGG 1 cut(s) 158
HgaI GACGC 1 cut(s) 32
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 1 cut(s) 153
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 158
Hpy166II GTNNAC 1 cut(s) 254
Hpy188I TCNGA 2 cut(s) 57, 262
Hpy188III TCNNGA 1 cut(s) 20
Hpy8I GTNNAC 1 cut(s) 254
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 1 cut(s) 181
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 245
HpyF3I CTNAG 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 1 cut(s) 153
Kzo9I GATC 1 cut(s) 52
LmnI GCTCC 1 cut(s) 263
MaeI CTAG 1 cut(s) 200
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 2 cut(s) 147, 182
MhlI GDGCHC 2 cut(s) 100, 241
MluCI AATT 3 cut(s) 31, 141, 277
MnlI CCTC 2 cut(s) 51, 157
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 3 cut(s) 47, 159, 235
MvaI CCWGG 2 cut(s) 47, 235
MwoI GCNNNNNNNGC 2 cut(s) 95, 245
NciI CCSGG 1 cut(s) 159
NdeII GATC 1 cut(s) 52
NlaIII CATG 1 cut(s) 153
NlaIV GGNNCC 2 cut(s) 98, 99
NmeAIII GCCGAG 1 cut(s) 52
PfeI GAWTC 1 cut(s) 5
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 1 cut(s) 169
Psp6I CCWGG 2 cut(s) 45, 233
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 2 cut(s) 45, 233
PspN4I GGNNCC 2 cut(s) 98, 99
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 3 cut(s) 96, 97, 146
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 1 cut(s) 52
Sau96I GGNCC 3 cut(s) 96, 97, 146
ScrFI CCNGG 3 cut(s) 47, 159, 235
SduI GDGCHC 2 cut(s) 100, 241
SetI ASST 2 cut(s) 51, 62
Sse9I AATT 3 cut(s) 31, 141, 277
SsiI CCGC 1 cut(s) 168
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 3 cut(s) 45, 157, 233
TaqI TCGA 2 cut(s) 21, 51
TasI AATT 3 cut(s) 31, 141, 277
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 1 cut(s) 5
XapI RAATTY 1 cut(s) 31
XmiI GTMKAC 1 cut(s) 253
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.