pycom12401g00280

cytochrome c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012401
OG0019579
Physical Location & Seq
Forward (+)
171598 .. 172948
1351 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12401g00280.2

Sequence Viewer

Length: 1307 bp
ATGTCAATATATGAATTGTTTCATTATTCGTTATTTCCGGGTCTTTTCATTGCATTCACTTACAACAAGAAACAACCACCAGCGTTTGGTGCAGCACCTGCATTTTGGTGCATTCTTCTTTCTTTCCTTGGTCTTTCGTTCCGTCATATTCCTAATAACTTATCCAATTACAACGTATTAACCGCTAATGCACCTTTCTTTTATCAAATCTCAGGGACATGGTCTAATCATGAGGGTAGTATTTTATCATGGTGTCGGATCCCAAGTTTATATGGATTCCTTCTTTGTTACCGGGGTCGACCCCAAAGCCATAATGTCTCAAAACGAGGAGGCCATAGAGAAACTCTTTTTTATTCCTTTGTCTCGAACTTCGTGAAGAACTCCATTCTATCTCTCCCTCGTTACGAACAAAAAAGTGGTGGTGTTGTACACCGCCAGTTGTACACCCCCTTCGTTCTACGAACCCTTGTTGATTCTGAACTTCGTTCGCGAAGGAACCGGGCTTTTGACGGGCCAGCCCTTTTTTATGCGCCGCTTTACCCCGAAAGGAAAATTAGCTTTGCTCCTCTGGGCACTAGGCGCTCCCGTGGTTCGCGAGAAGGAAAAAGGATGAATCCTTTGTTACATCTGGCACGAGATGATAAAGAGAGAGCTTCGTCTATCAATGAACAGCGGATTGACGGAGCTCTTGGCATTGCTTTGTTTTTCTCTCCTTTCCTATCAGCGAGTTCCGATCCTTTTGTTCGAAATTTCTTCGTTCGTACCGAACCGCTTGCAGAATCAAATCCTGTTCCACAAGATCCTATATCAGCTATACATCCTCCTTGCATTTATGCCGGAGACGTCGCCAGTGCTATGGGCTTTGGCTTATGTAGATCAAAAATGATGAATGGGATTGTGGCACTCCACTCGCCGCCAATGCGGAAGGATGCCGTCGAAAAGAATGGAACGCTGCTTCGCTCTGCTGGATGCGTCGGATCCCGTACAACAAGCGAGCTTTTTACCCTCAAATTCAAACATGTGGGCGCAAAATGCTATCCTGCTCTATTGTTGCGTAGCAATAGAAGCCTGCTCGTGCAGCTTCGGCGGCGCTTTTTCGCCTTCTCTTCGCTCTGGGCAGGAGCGCTAGTGGACACGGGGAGGAGCAGGCGAAGCGTGTCGTTCGTAATGGACAGAAAGATACCACCACTTCGCCTCTTTGTTGGACTTCGAATTTGGATCTTGACATTCGGTGGTTTTTTAACCGTAGGCATCTTGCCAGGAAGTTGGTGGGCTCATCATGAATTAGGTCGGGGTGCTGGTGGTTT
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_C_asm

Protein Analysis

435

Amino Acids

48.85

Weight (kDa)

10.43

Isoelectric Point (pI)

55.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019579)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00830
malus_domestica MD14G1092300.v1.1
pyrus_communis pycom12401g00280
rosa_laevigata RLG00000000135 RLG00000000147 RLG00000000477 RLG00000000492

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 106
AatII GACGTC 1 cut(s) 846
Acc36I ACCTGC 1 cut(s) 106
AccB7I CCANNNNNTGG 1 cut(s) 86
AccI GTMKAC 1 cut(s) 298
AccII CGCG 2 cut(s) 490, 595
AciI CCGC 8 cut(s) 183, 433, 533, 673, 770, 914, 922, 1087
AclWI GGATC 7 cut(s) 253, 266, 728, 794, 972, 985, 1226
AcsI RAATTY 3 cut(s) 748, 1010, 1212
AcyI GRCGYC 1 cut(s) 843
AfaI GTAC 4 cut(s) 429, 443, 763, 985
AfeI AGCGCT 1 cut(s) 1125
AfiI CCNNNNNNNGG 1 cut(s) 86
AflIII ACRYGT 1 cut(s) 1018
AgsI TTSAA 1 cut(s) 1015
AjnI CCWGG 1 cut(s) 1258
AluBI AGCT 6 cut(s) 558, 653, 686, 812, 997, 1081
AluI AGCT 6 cut(s) 558, 653, 686, 812, 997, 1081
Alw21I GWGCWC 1 cut(s) 688
Alw26I GTCTC 3 cut(s) 322, 367, 834
AlwI GGATC 7 cut(s) 253, 266, 728, 794, 972, 985, 1226
AlwNI CAGNNNCTG 1 cut(s) 98
Aor51HI AGCGCT 1 cut(s) 1125
AoxI GGCC 2 cut(s) 331, 512
ApeKI GCWGC 3 cut(s) 92, 952, 1078
ApoI RAATTY 3 cut(s) 748, 1010, 1212
ArsI GACNNNNNNTTYG 2 cut(s) 1197, 1229
Asp700I GAANNNNTTC 1 cut(s) 18
AspLEI GCGC 5 cut(s) 532, 582, 1028, 1092, 1126
AspS9I GGNCC 1 cut(s) 512
AsuC2I CCSGG 3 cut(s) 39, 293, 500
AsuII TTCGAA 2 cut(s) 745, 1210
BaeGI GKGCMC 1 cut(s) 575
BamHI GGATCC 2 cut(s) 258, 977
BanII GRGCYC 2 cut(s) 688, 1276
BarI GAAGNNNNNNTAC 2 cut(s) 434, 466
BauI CACGAG 2 cut(s) 633, 1073
Bbv12I GWGCWC 1 cut(s) 688
BbvI GCAGC 3 cut(s) 104, 939, 1090
BceAI ACGGC 1 cut(s) 917
BcgI CGANNNNNNTGC 4 cut(s) 755, 789, 891, 925
BciT130I CCWGG 1 cut(s) 1260
BcnI CCSGG 3 cut(s) 39, 293, 500
BcoDI GTCTC 3 cut(s) 322, 367, 834
BfaI CTAG 2 cut(s) 576, 1127
BfoI RGCGCY 3 cut(s) 583, 1093, 1127
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 6 cut(s) 93, 533, 914, 953, 1079, 1088
BlsI GCNGC 6 cut(s) 94, 534, 915, 954, 1080, 1089
Bme1390I CCNGG 4 cut(s) 39, 293, 500, 1260
BmgT120I GGNCC 1 cut(s) 512
BmiI GGNNCC 3 cut(s) 260, 497, 979
BmrFI CCNGG 4 cut(s) 39, 293, 500, 1260
BmsI GCATC 3 cut(s) 919, 959, 1260
Bpu14I TTCGAA 2 cut(s) 745, 1210
BpuMI CCSGG 3 cut(s) 39, 293, 500
BsaHI GRCGYC 1 cut(s) 843
BsaJI CCNNGG 3 cut(s) 127, 292, 586
BsaXI ACNNNNNCTCC 2 cut(s) 1113, 1143
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 436, 849
Bse3DI GCAATG 2 cut(s) 48, 693
BseBI CCWGG 1 cut(s) 1260
BseDI CCNNGG 3 cut(s) 127, 292, 586
BseGI GGATG 4 cut(s) 615, 817, 934, 974
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 2 cut(s) 48, 693
BseMII CTCAG 1 cut(s) 225
BseNI ACTGG 2 cut(s) 436, 849
BseRI GAGGAG 3 cut(s) 342, 555, 1156
BseSI GKGCMC 1 cut(s) 575
BseXI GCAGC 3 cut(s) 104, 939, 1090
BsgI GTGCAG 2 cut(s) 111, 1097
Bsh1236I CGCG 2 cut(s) 490, 595
BshFI GGCC 2 cut(s) 333, 514
BsiHKAI GWGCWC 1 cut(s) 688
BsiSI CCGG 4 cut(s) 38, 292, 499, 837
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 3 cut(s) 322, 367, 834
BsmBI CGTCTC 1 cut(s) 834
BsmFI GGGAC 1 cut(s) 229
BsmI GAATGC 2 cut(s) 53, 111
BsnI GGCC 2 cut(s) 333, 514
Bsp119I TTCGAA 2 cut(s) 745, 1210
Bsp1286I GDGCHC 3 cut(s) 575, 688, 1276
Bsp1407I TGTACA 2 cut(s) 427, 441
Bsp143I GATC 6 cut(s) 258, 733, 799, 875, 977, 1218
Bsp68I TCGCGA 2 cut(s) 490, 595
BspACI CCGC 8 cut(s) 183, 433, 533, 673, 770, 914, 922, 1087
BspANI GGCC 2 cut(s) 333, 514
BspCNI CTCAG 1 cut(s) 224
BspFNI CGCG 2 cut(s) 490, 595
BspHI TCATGA 2 cut(s) 229, 1279
BspLI GGNNCC 3 cut(s) 260, 497, 979
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 7 cut(s) 253, 266, 728, 794, 972, 985, 1226
BspT104I TTCGAA 2 cut(s) 745, 1210
BsrDI GCAATG 2 cut(s) 48, 693
BsrGI TGTACA 2 cut(s) 427, 441
BsrI ACTGG 2 cut(s) 436, 849
BssECI CCNNGG 3 cut(s) 127, 292, 586
BssMI GATC 6 cut(s) 258, 733, 799, 875, 977, 1218
BssNI GRCGYC 1 cut(s) 843
BssSI CACGAG 2 cut(s) 633, 1073
BssT1I CCWWGG 1 cut(s) 127
Bst2BI CACGAG 2 cut(s) 633, 1073
Bst2UI CCWGG 1 cut(s) 1260
Bst4CI ACNGT 1 cut(s) 1246
Bst6I CTCTTC 1 cut(s) 1111
BstACI GRCGYC 1 cut(s) 843
BstAPI GCANNNNNTGC 1 cut(s) 98
BstAUI TGTACA 2 cut(s) 427, 441
BstBI TTCGAA 2 cut(s) 745, 1210
BstC8I GCNNGC 5 cut(s) 516, 774, 995, 1070, 1148
BstDEI CTNAG 1 cut(s) 211
BstDSI CCRYGG 1 cut(s) 586
BstF5I GGATG 4 cut(s) 615, 817, 934, 974
BstFNI CGCG 2 cut(s) 490, 595
BstH2I RGCGCY 3 cut(s) 583, 1093, 1127
BstHHI GCGC 5 cut(s) 532, 582, 1028, 1092, 1126
BstKTI GATC 6 cut(s) 261, 736, 802, 878, 980, 1221
BstMAI GTCTC 3 cut(s) 322, 367, 834
BstMBI GATC 6 cut(s) 258, 733, 799, 875, 977, 1218
BstNI CCWGG 1 cut(s) 1260
BstNSI RCATGY 1 cut(s) 1022
BstSCI CCNGG 4 cut(s) 37, 291, 498, 1258
BstSLI GKGCMC 1 cut(s) 575
BstUI CGCG 2 cut(s) 490, 595
BstV1I GCAGC 3 cut(s) 104, 939, 1090
BstX2I RGATCY 4 cut(s) 258, 799, 977, 1218
BstXI CCANNNNNNTGG 2 cut(s) 856, 1266
BstYI RGATCY 4 cut(s) 258, 799, 977, 1218
BsuRI GGCC 2 cut(s) 333, 514
BtgI CCRYGG 1 cut(s) 586
BtsCI GGATG 4 cut(s) 615, 817, 934, 974
BtsIMutI CAGTG 1 cut(s) 856
BtuMI TCGCGA 2 cut(s) 490, 595
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 5 cut(s) 516, 774, 995, 1070, 1148
CaiI CAGNNNCTG 1 cut(s) 98
CciI TCATGA 2 cut(s) 229, 1279
CfoI GCGC 5 cut(s) 532, 582, 1028, 1092, 1126
Cfr13I GGNCC 1 cut(s) 512
CseI GACGC 1 cut(s) 961
Csp6I GTAC 4 cut(s) 428, 442, 762, 984
CviAII CATG 5 cut(s) 219, 230, 249, 1019, 1280
CviQI GTAC 4 cut(s) 428, 442, 762, 984
DdeI CTNAG 1 cut(s) 211
DpnI GATC 6 cut(s) 260, 735, 801, 877, 979, 1220
DpnII GATC 6 cut(s) 258, 733, 799, 875, 977, 1218
Eam1104I CTCTTC 1 cut(s) 1111
EarI CTCTTC 1 cut(s) 1111
Ecl136II GAGCTC 1 cut(s) 686
Eco130I CCWWGG 1 cut(s) 127
Eco24I GRGCYC 2 cut(s) 688, 1276
Eco47III AGCGCT 1 cut(s) 1125
Eco53kI GAGCTC 1 cut(s) 686
EcoICRI GAGCTC 1 cut(s) 686
EcoRII CCWGG 1 cut(s) 1258
EcoT14I CCWWGG 1 cut(s) 127
EcoT38I GRGCYC 2 cut(s) 688, 1276
ErhI CCWWGG 1 cut(s) 127
Esp3I CGTCTC 1 cut(s) 834
FaeI CATG 5 cut(s) 222, 233, 252, 1022, 1283
FaqI GGGAC 1 cut(s) 229
FatI CATG 5 cut(s) 218, 229, 248, 1018, 1279
FblI GTMKAC 1 cut(s) 298
Fnu4HI GCNGC 6 cut(s) 93, 533, 914, 953, 1079, 1088
FokI GGATG 4 cut(s) 622, 804, 941, 981
FriOI GRGCYC 2 cut(s) 688, 1276
Fsp4HI GCNGC 6 cut(s) 93, 533, 914, 953, 1079, 1088
FspBI CTAG 2 cut(s) 576, 1127
GlaI GCGC 5 cut(s) 531, 581, 1027, 1091, 1125
GluI GCNGC 6 cut(s) 93, 533, 914, 953, 1079, 1088
HaeII RGCGCY 3 cut(s) 583, 1093, 1127
HaeIII GGCC 2 cut(s) 333, 514
HapII CCGG 4 cut(s) 38, 292, 499, 837
HgaI GACGC 1 cut(s) 961
HhaI GCGC 5 cut(s) 532, 582, 1028, 1092, 1126
Hin1I GRCGYC 1 cut(s) 843
Hin1II CATG 5 cut(s) 222, 233, 252, 1022, 1283
Hin6I GCGC 5 cut(s) 530, 580, 1026, 1090, 1124
HinP1I GCGC 5 cut(s) 530, 580, 1026, 1090, 1124
HincII GTYRAC 1 cut(s) 299
HindII GTYRAC 1 cut(s) 299
HinfI GANTC 4 cut(s) 276, 473, 613, 779
HpaII CCGG 4 cut(s) 38, 292, 499, 837
Hpy166II GTNNAC 4 cut(s) 299, 430, 444, 1132
Hpy188I TCNGA 4 cut(s) 258, 478, 733, 977
Hpy188III TCNNGA 7 cut(s) 230, 364, 373, 489, 594, 1222, 1280
Hpy8I GTNNAC 4 cut(s) 299, 430, 444, 1132
Hpy99I CGWCG 3 cut(s) 848, 938, 977
HpyAV CCTTC 6 cut(s) 290, 460, 486, 593, 919, 1111
HpyCH4III ACNGT 1 cut(s) 1246
HpyCH4IV ACGT 2 cut(s) 174, 843
HpyCH4V TGCA 8 cut(s) 53, 92, 101, 111, 191, 776, 828, 1078
HpyF3I CTNAG 1 cut(s) 211
HpySE526I ACGT 2 cut(s) 174, 843
Hsp92I GRCGYC 1 cut(s) 843
Hsp92II CATG 5 cut(s) 222, 233, 252, 1022, 1283
HspAI GCGC 5 cut(s) 530, 580, 1026, 1090, 1124
Kzo9I GATC 6 cut(s) 258, 733, 799, 875, 977, 1218
LmnI GCTCC 5 cut(s) 568, 587, 683, 1121, 1143
Lsp1109I GCAGC 3 cut(s) 104, 939, 1090
LweI GCATC 3 cut(s) 919, 959, 1260
MaeI CTAG 2 cut(s) 576, 1127
MaeII ACGT 2 cut(s) 174, 843
MaeIII GTNAC 3 cut(s) 287, 401, 621
MalI GATC 6 cut(s) 260, 735, 801, 877, 979, 1220
MboI GATC 6 cut(s) 258, 733, 799, 875, 977, 1218
MboII GAAGA 4 cut(s) 107, 388, 745, 1098
MflI RGATCY 4 cut(s) 258, 799, 977, 1218
MhlI GDGCHC 3 cut(s) 575, 688, 1276
MluCI AATT 7 cut(s) 14, 166, 552, 748, 1010, 1212, 1283
MmeI TCCRAC 3 cut(s) 236, 955, 1183
MnlI CCTC 9 cut(s) 226, 320, 323, 408, 576, 831, 1016, 1134, 1205
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 2 cut(s) 179, 1241
MslI CAYNNNNRTG 1 cut(s) 106
MspA1I CMGCKG 1 cut(s) 673
MspI CCGG 4 cut(s) 38, 292, 499, 837
MspR9I CCNGG 4 cut(s) 39, 293, 500, 1260
Mva1269I GAATGC 2 cut(s) 53, 111
MvaI CCWGG 1 cut(s) 1260
MvnI CGCG 2 cut(s) 490, 595
NciI CCSGG 3 cut(s) 39, 293, 500
NdeII GATC 6 cut(s) 258, 733, 799, 875, 977, 1218
NlaIII CATG 5 cut(s) 222, 233, 252, 1022, 1283
NlaIV GGNNCC 3 cut(s) 260, 497, 979
NruI TCGCGA 2 cut(s) 490, 595
NspI RCATGY 1 cut(s) 1022
NspV TTCGAA 2 cut(s) 745, 1210
PagI TCATGA 2 cut(s) 229, 1279
PaqCI CACCTGC 1 cut(s) 106
PciI ACATGT 1 cut(s) 1018
PcsI WCGNNNNNNNCGW 1 cut(s) 762
PctI GAATGC 2 cut(s) 53, 111
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 4 cut(s) 276, 473, 613, 779
PflFI GACNNNGTC 1 cut(s) 220
PflMI CCANNNNNTGG 1 cut(s) 86
PkrI GCNGC 6 cut(s) 94, 534, 915, 954, 1080, 1089
PscI ACATGT 1 cut(s) 1018
Psp124BI GAGCTC 1 cut(s) 688
Psp6I CCWGG 1 cut(s) 1258
PspGI CCWGG 1 cut(s) 1258
PspN4I GGNNCC 3 cut(s) 260, 497, 979
PspPI GGNCC 1 cut(s) 512
PstNI CAGNNNCTG 1 cut(s) 98
PsuI RGATCY 4 cut(s) 258, 799, 977, 1218
PsyI GACNNNGTC 1 cut(s) 220
RruI TCGCGA 2 cut(s) 490, 595
RsaI GTAC 4 cut(s) 429, 443, 763, 985
RsaNI GTAC 4 cut(s) 428, 442, 762, 984
RseI CAYNNNNRTG 1 cut(s) 106
SacI GAGCTC 1 cut(s) 688
SalI GTCGAC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 179, 1241
SatI GCNGC 6 cut(s) 93, 533, 914, 953, 1079, 1088
Sau3AI GATC 6 cut(s) 258, 733, 799, 875, 977, 1218
Sau96I GGNCC 1 cut(s) 512
ScrFI CCNGG 4 cut(s) 39, 293, 500, 1260
SduI GDGCHC 3 cut(s) 575, 688, 1276
SfaNI GCATC 3 cut(s) 919, 959, 1260
SfuI TTCGAA 2 cut(s) 745, 1210
SmiMI CAYNNNNRTG 1 cut(s) 106
Sse9I AATT 7 cut(s) 14, 166, 552, 748, 1010, 1212, 1283
SsiI CCGC 8 cut(s) 183, 433, 533, 673, 770, 914, 922, 1087
SspMI CTAG 2 cut(s) 576, 1127
SstI GAGCTC 1 cut(s) 688
StyD4I CCNGG 4 cut(s) 37, 291, 498, 1258
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 1 cut(s) 1246
TaiI ACGT 2 cut(s) 177, 846
TaqI TCGA 5 cut(s) 298, 365, 745, 936, 1210
TasI AATT 7 cut(s) 14, 166, 552, 748, 1010, 1212, 1283
TatI WGTACW 2 cut(s) 427, 441
TauI GCSGC 3 cut(s) 535, 916, 1090
TfiI GAWTC 4 cut(s) 276, 473, 613, 779
Tru1I TTAA 2 cut(s) 179, 1241
Tru9I TTAA 2 cut(s) 179, 1241
TscAI CASTG 1 cut(s) 856
TseI GCWGC 3 cut(s) 92, 952, 1078
TspDTI ATGAA 7 cut(s) 11, 27, 37, 626, 681, 902, 1296
TspGWI ACGGA 2 cut(s) 131, 696
TspRI CASTG 1 cut(s) 856
Tth111I GACNNNGTC 1 cut(s) 220
Van91I CCANNNNNTGG 1 cut(s) 86
XapI RAATTY 3 cut(s) 748, 1010, 1212
XceI RCATGY 1 cut(s) 1022
XcmI CCANNNNNNNNNTGG 1 cut(s) 1266
XmiI GTMKAC 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 2 cut(s) 576, 1127
ZraI GACGTC 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.