pycom12g00220

HNH endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012307
Physical Location & Seq
Reverse (-)
302336 .. 306154
3819 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 897 bp
ATGGCTGCACAGTTGTCGTCGACAGCACAAGGAGGAGGAGGAGGACGACGAGTGAAGTTGGCCTGCAGTGGGGGTTGGGGGTTGGGGTTGGATCAAAACATGCAGCTCTTCACATCAATCCAACCCCACAAGCTCCTCAGGCGCAGGCGCTGCCTCAGATCATTGGATTCTACCAGGCTTTACTCTTCTTCTTCTTCTAGGAACAAGGCGAAACGCTTCAACTCCGAGGCCCATTTGTTTGTCGATGGTGGTGATCATGATGAGGGAGACGGTGTTCATGGCGGTGACGACGATGGTGATCATGTTGAGTACTATGAGAGGGACGATGACTTGTCTTGCTTCAGAGGTCTCGTCTTGGATATCTCCTACAGGCCGGTTAATGTTGTATGCTGGAAGCGTGCTATTTGCTTGGAATTCATGGAGAAGGCTGATGTACTTGAATACTATGACCGGACTGTAAACTCCCCAAGCGGATCCTACTACATCCCGGCTGTCCTAAGGGTTCCCCATTTACTGCAGATTGTAAAGAGAAGAAGAATTAAGAACAGTCTCAGTCGTAAAAATATACTATGTCGGGACAGTTTCACTTGTCAATACTGCTCTTCACGTGAAAACTTGACTATTGATCATGTTTTCCCAATTGCACGCGGTGGACAGTGGAAGTGGGATAATCTGGTCACCGCATGTGCAAGGTGCAACTCGAAGAAAGGTCAGAAGACGTTGGAAGAAGCAAATATGAAGCTGATGAAAGTCCCCAAGGCTCCGAAGGACTATGACATACTTGCAATACCACTAACAAGTGCAGCAATTAAAATGCTGAAAATGAGAAAGGGAACTCCAGAAGAGTGGCTCCAATATCTATCAAATTCCAAGCCGCCGCCTTCTTCAGAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH_5

Protein Analysis

299

Amino Acids

33.64

Weight (kDa)

9.23

Isoelectric Point (pI)

63.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HNH_5 PF14279 197 - 248 2.2e-17 HNH endonuclease
HNH PF01844 197 - 238 2.9e-13 HNH endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012307)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23840
fragaria_vesca FvH4_3g25250 FvH4_3g25250
malus_domestica MD12G1002500.v1.1
prunus_persica Prupe.7G002400_v2.0.a1 Prupe.7G002400_v2.0.a1 Prupe.7G002400_v2.0.a1 Prupe.7G002400_v2.0.a1
pyrus_communis pycom12g00220
rosa_chinensis RchiOBHm_Chr5g0046891
rosa_laevigata RLG00000034453
rosa_multiflora Rmu_sc0005233.1_g000003
rosa_roxburghii Rroxscaffold_1G00034500
rosa_rugosa Rorug05G0232400
rosa_samantha Rh5AG311900 Rh5BG320900 Rh5CG347100 Rh5DG331600
rosa_wichuraiana Rw5G029150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 20
AccII CGCG 1 cut(s) 648
AciI CCGC 6 cut(s) 282, 471, 648, 681, 875, 878
AclWI GGATC 3 cut(s) 99, 468, 481
AcsI RAATTY 2 cut(s) 413, 865
AcuI CTGAAG 2 cut(s) 325, 870
AcvI CACGTG 1 cut(s) 608
AdeI CACNNNGTG 1 cut(s) 650
AfaI GTAC 2 cut(s) 311, 435
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 2 cut(s) 220, 440
AjnI CCWGG 1 cut(s) 173
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 3 cut(s) 106, 133, 742
AluI AGCT 3 cut(s) 106, 133, 742
Alw26I GTCTC 3 cut(s) 261, 353, 554
AlwI GGATC 3 cut(s) 99, 468, 481
AlwNI CAGNNNCTG 1 cut(s) 150
AoxI GGCC 3 cut(s) 60, 228, 371
ApeKI GCWGC 4 cut(s) 5, 103, 150, 803
ApoI RAATTY 2 cut(s) 413, 865
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 2 cut(s) 144, 150
AspS9I GGNCC 1 cut(s) 229
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 4 cut(s) 263, 296, 308, 670
AxyI CCTNAGG 2 cut(s) 137, 497
BamHI GGATCC 1 cut(s) 473
BbrPI CACGTG 1 cut(s) 608
BbsI GAAGAC 1 cut(s) 722
BbvI GCAGC 3 cut(s) 115, 137, 815
BccI CCATC 2 cut(s) 239, 287
BcgI CGANNNNNNTGC 1 cut(s) 31
BciT130I CCWGG 1 cut(s) 175
BclI TGATCA 3 cut(s) 253, 298, 625
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 3 cut(s) 261, 353, 554
BfaI CTAG 1 cut(s) 198
BfmI CTRYAG 3 cut(s) 64, 367, 515
BfoI RGCGCY 1 cut(s) 151
BisI GCNGC 6 cut(s) 6, 104, 151, 804, 875, 878
BlsI GCNGC 6 cut(s) 7, 105, 152, 805, 876, 879
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 2 cut(s) 175, 488
BmgT120I GGNCC 1 cut(s) 229
BmiI GGNNCC 4 cut(s) 475, 504, 762, 851
BmrFI CCNGG 2 cut(s) 175, 488
BpiI GAAGAC 1 cut(s) 722
BpmI CTGGAG 1 cut(s) 822
BpuMI CCSGG 1 cut(s) 488
BsaAI YACGTR 1 cut(s) 608
BsaBI GATNNNNATC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 353
BsaJI CCNNGG 2 cut(s) 225, 756
BsaWI WCCGGW 1 cut(s) 450
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse118I RCCGGY 1 cut(s) 373
Bse21I CCTNAGG 2 cut(s) 137, 497
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 1 cut(s) 175
BseDI CCNNGG 2 cut(s) 225, 756
BseGI GGATG 1 cut(s) 483
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 3 cut(s) 151, 169, 565
BseRI GAGGAG 4 cut(s) 48, 51, 54, 125
BseXI GCAGC 3 cut(s) 115, 137, 815
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 1 cut(s) 648
BshFI GGCC 3 cut(s) 62, 230, 373
BsiSI CCGG 3 cut(s) 374, 451, 488
BslFI GGGAC 3 cut(s) 335, 590, 737
BslI CCNNNNNNNGG 1 cut(s) 69
BsmAI GTCTC 3 cut(s) 261, 353, 554
BsmBI CGTCTC 1 cut(s) 261
BsmFI GGGAC 3 cut(s) 335, 590, 737
BsnI GGCC 3 cut(s) 62, 230, 373
Bso31I GGTCTC 1 cut(s) 353
Bsp143I GATC 6 cut(s) 91, 158, 253, 298, 473, 625
BspACI CCGC 6 cut(s) 282, 471, 648, 681, 875, 878
BspANI GGCC 3 cut(s) 62, 230, 373
BspCNI CTCAG 3 cut(s) 150, 168, 564
BspFNI CGCG 1 cut(s) 648
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 4 cut(s) 475, 504, 762, 851
BspMAI CTGCAG 2 cut(s) 68, 519
BspPI GGATC 3 cut(s) 99, 468, 481
BspQI GCTCTTC 2 cut(s) 113, 607
BspTNI GGTCTC 1 cut(s) 353
BsrFI RCCGGY 1 cut(s) 373
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 2 cut(s) 225, 756
BssMI GATC 6 cut(s) 91, 158, 253, 298, 473, 625
BssT1I CCWWGG 1 cut(s) 756
Bst2UI CCWGG 1 cut(s) 175
Bst4CI ACNGT 6 cut(s) 12, 272, 457, 548, 581, 657
Bst6I CTCTTC 4 cut(s) 113, 190, 607, 837
BstAPI GCANNNNNTGC 1 cut(s) 150
BstBAI YACGTR 1 cut(s) 608
BstC8I GCNNGC 4 cut(s) 64, 146, 399, 646
BstDEI CTNAG 4 cut(s) 137, 155, 497, 551
BstEII GGTNACC 1 cut(s) 676
BstF5I GGATG 1 cut(s) 483
BstFNI CGCG 1 cut(s) 648
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 2 cut(s) 144, 150
BstKTI GATC 6 cut(s) 94, 161, 256, 301, 476, 628
BstMAI GTCTC 3 cut(s) 261, 353, 554
BstMBI GATC 6 cut(s) 91, 158, 253, 298, 473, 625
BstMWI GCNNNNNNNGC 2 cut(s) 139, 150
BstNI CCWGG 1 cut(s) 175
BstNSI RCATGY 2 cut(s) 103, 687
BstPI GGTNACC 1 cut(s) 676
BstSCI CCNGG 2 cut(s) 173, 486
BstSFI CTRYAG 3 cut(s) 64, 367, 515
BstUI CGCG 1 cut(s) 648
BstV1I GCAGC 3 cut(s) 115, 137, 815
BstV2I GAAGAC 1 cut(s) 722
BstX2I RGATCY 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 846
BstYI RGATCY 1 cut(s) 473
Bsu36I CCTNAGG 2 cut(s) 137, 497
BsuRI GGCC 3 cut(s) 62, 230, 373
BtsCI GGATG 1 cut(s) 483
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 2 cut(s) 73, 662
Cac8I GCNNGC 4 cut(s) 64, 146, 399, 646
CaiI CAGNNNCTG 1 cut(s) 150
CciI TCATGA 1 cut(s) 256
CfoI GCGC 2 cut(s) 144, 150
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 1 cut(s) 229
Csp6I GTAC 2 cut(s) 310, 434
CviAII CATG 8 cut(s) 100, 257, 278, 302, 418, 629, 684, 894
CviQI GTAC 2 cut(s) 310, 434
DdeI CTNAG 4 cut(s) 137, 155, 497, 551
DpnI GATC 6 cut(s) 93, 160, 255, 300, 475, 627
DpnII GATC 6 cut(s) 91, 158, 253, 298, 473, 625
DraIII CACNNNGTG 1 cut(s) 650
Eam1104I CTCTTC 4 cut(s) 113, 190, 607, 837
EarI CTCTTC 4 cut(s) 113, 190, 607, 837
Eco130I CCWWGG 1 cut(s) 756
Eco31I GGTCTC 1 cut(s) 353
Eco32I GATATC 1 cut(s) 361
Eco57I CTGAAG 2 cut(s) 325, 870
Eco72I CACGTG 1 cut(s) 608
Eco81I CCTNAGG 2 cut(s) 137, 497
Eco91I GGTNACC 1 cut(s) 676
EcoO65I GGTNACC 1 cut(s) 676
EcoRI GAATTC 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 173
EcoRV GATATC 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 756
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 8 cut(s) 103, 260, 281, 305, 421, 632, 687, 897
FaqI GGGAC 3 cut(s) 335, 590, 737
FatI CATG 8 cut(s) 99, 256, 277, 301, 417, 628, 683, 893
FbaI TGATCA 3 cut(s) 253, 298, 625
FblI GTMKAC 1 cut(s) 20
Fnu4HI GCNGC 6 cut(s) 6, 104, 151, 804, 875, 878
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 6 cut(s) 6, 104, 151, 804, 875, 878
FspBI CTAG 1 cut(s) 198
GlaI GCGC 2 cut(s) 143, 149
GluI GCNGC 6 cut(s) 6, 104, 151, 804, 875, 878
GsuI CTGGAG 1 cut(s) 822
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 3 cut(s) 62, 230, 373
HapII CCGG 3 cut(s) 374, 451, 488
HhaI GCGC 2 cut(s) 144, 150
Hin1II CATG 8 cut(s) 103, 260, 281, 305, 421, 632, 687, 897
Hin6I GCGC 2 cut(s) 142, 148
HinP1I GCGC 2 cut(s) 142, 148
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 1 cut(s) 167
HpaII CCGG 3 cut(s) 374, 451, 488
HphI GGTGA 4 cut(s) 263, 296, 308, 670
Hpy166II GTNNAC 3 cut(s) 21, 460, 653
Hpy188I TCNGA 6 cut(s) 158, 226, 344, 714, 765, 889
Hpy188III TCNNGA 3 cut(s) 257, 575, 839
Hpy8I GTNNAC 3 cut(s) 21, 460, 653
Hpy99I CGWCG 3 cut(s) 22, 51, 293
HpyAV CCTTC 3 cut(s) 418, 760, 891
HpyCH4III ACNGT 6 cut(s) 12, 272, 457, 548, 581, 657
HpyCH4IV ACGT 2 cut(s) 607, 719
HpyCH4V TGCA 9 cut(s) 8, 66, 103, 517, 644, 689, 696, 785, 803
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 150
HpyF3I CTNAG 4 cut(s) 137, 155, 497, 551
HpySE526I ACGT 2 cut(s) 607, 719
Hsp92II CATG 8 cut(s) 103, 260, 281, 305, 421, 632, 687, 897
HspAI GCGC 2 cut(s) 142, 148
Ksp22I TGATCA 3 cut(s) 253, 298, 625
Kzo9I GATC 6 cut(s) 91, 158, 253, 298, 473, 625
LguI GCTCTTC 2 cut(s) 113, 607
LmnI GCTCC 3 cut(s) 138, 766, 855
Lsp1109I GCAGC 3 cut(s) 115, 137, 815
MaeI CTAG 1 cut(s) 198
MaeII ACGT 2 cut(s) 607, 719
MaeIII GTNAC 2 cut(s) 284, 676
MalI GATC 6 cut(s) 93, 160, 255, 300, 475, 627
MboI GATC 6 cut(s) 91, 158, 253, 298, 473, 625
MfeI CAATTG 1 cut(s) 639
MflI RGATCY 1 cut(s) 473
MluCI AATT 5 cut(s) 413, 537, 639, 807, 865
MmeI TCCRAC 3 cut(s) 69, 145, 702
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 3 cut(s) 378, 540, 810
MslI CAYNNNNRTG 1 cut(s) 282
MspI CCGG 3 cut(s) 374, 451, 488
MspR9I CCNGG 2 cut(s) 175, 488
MunI CAATTG 1 cut(s) 639
MvaI CCWGG 1 cut(s) 175
MvnI CGCG 1 cut(s) 648
MwoI GCNNNNNNNGC 2 cut(s) 139, 150
NciI CCSGG 1 cut(s) 488
NdeII GATC 6 cut(s) 91, 158, 253, 298, 473, 625
NlaIII CATG 8 cut(s) 103, 260, 281, 305, 421, 632, 687, 897
NlaIV GGNNCC 4 cut(s) 475, 504, 762, 851
NmuCI GTSAC 2 cut(s) 284, 676
NspI RCATGY 2 cut(s) 103, 687
PagI TCATGA 1 cut(s) 256
PciSI GCTCTTC 2 cut(s) 113, 607
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 167
PkrI GCNGC 6 cut(s) 7, 105, 152, 805, 876, 879
PmaCI CACGTG 1 cut(s) 608
PmlI CACGTG 1 cut(s) 608
Ppu21I YACGTR 1 cut(s) 608
Psp6I CCWGG 1 cut(s) 173
PspCI CACGTG 1 cut(s) 608
PspEI GGTNACC 1 cut(s) 676
PspGI CCWGG 1 cut(s) 173
PspN4I GGNNCC 4 cut(s) 475, 504, 762, 851
PspPI GGNCC 1 cut(s) 229
PstI CTGCAG 2 cut(s) 68, 519
PstNI CAGNNNCTG 1 cut(s) 150
PsuI RGATCY 1 cut(s) 473
RsaI GTAC 2 cut(s) 311, 435
RsaNI GTAC 2 cut(s) 310, 434
RseI CAYNNNNRTG 1 cut(s) 282
SalI GTCGAC 1 cut(s) 19
SapI GCTCTTC 2 cut(s) 113, 607
SaqAI TTAA 3 cut(s) 378, 540, 810
SatI GCNGC 6 cut(s) 6, 104, 151, 804, 875, 878
Sau3AI GATC 6 cut(s) 91, 158, 253, 298, 473, 625
Sau96I GGNCC 1 cut(s) 229
ScaI AGTACT 1 cut(s) 311
ScrFI CCNGG 2 cut(s) 175, 488
SetI ASST 8 cut(s) 108, 135, 349, 610, 695, 712, 722, 744
SfcI CTRYAG 3 cut(s) 64, 367, 515
SmiMI CAYNNNNRTG 1 cut(s) 282
Sse9I AATT 5 cut(s) 413, 537, 639, 807, 865
SsiI CCGC 6 cut(s) 282, 471, 648, 681, 875, 878
SspMI CTAG 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 173, 486
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 6 cut(s) 12, 272, 457, 548, 581, 657
TaiI ACGT 2 cut(s) 610, 722
TaqI TCGA 3 cut(s) 20, 243, 701
TasI AATT 5 cut(s) 413, 537, 639, 807, 865
TatI WGTACW 2 cut(s) 309, 433
TauI GCSGC 2 cut(s) 877, 880
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 3 cut(s) 378, 540, 810
Tru9I TTAA 3 cut(s) 378, 540, 810
TscAI CASTG 2 cut(s) 73, 662
TseFI GTSAC 2 cut(s) 284, 676
TseI GCWGC 4 cut(s) 5, 103, 150, 803
Tsp45I GTSAC 2 cut(s) 284, 676
TspDTI ATGAA 4 cut(s) 266, 406, 752, 761
TspRI CASTG 2 cut(s) 73, 662
XapI RAATTY 2 cut(s) 413, 865
XceI RCATGY 2 cut(s) 103, 687
XmiI GTMKAC 1 cut(s) 20
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 198
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.