pycom12g07660

L-galactono-1,4-lactone dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0029424
Physical Location & Seq
Reverse (-)
8234509 .. 8236687
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g07660.1

Sequence Viewer

Length: 675 bp
ATGAATGAGCTCTCATTTACTGAGTTAAGAGATAAACTACTTGCCCTAGATCCTCTCGACAAAGATCACGTTGCAAAGGTTAACCAAGCTGAGGCTGAGTTCTGGAAGAAGTCGGAGGGATACAGAGTGGGATGGAGTGATGAAATTTTGGGCTTTGATTGTGGTGGTCAACAGTGGGTCTCAGAGACTTGTTTTCCAGCAGGAACCCTTGCCAAACCAAGCATGAAAGACCTTGAATACATAGAAGGTGTAAAGCAGCTCATCGAGAAGAATGAGATACCTGCACCTGCTCCAATAGAGCAGCGCTGGACCGCCTGCACTAAGAGCCCCATGAGTCCAGCTTCAAGCACAAGAGAGGATGATATATTTTCATGGGTTGGTATAATCATGTACCTTCCTACAACAGATCCCCGCCAAAGGAAGGAAATTACAGAAGAGTTTTTTCACTACAGGCGACTGACCCAGACACAATTGTGGGATAAGTATTCCGCTTATGAACACTGGGCTAAGATTGAGGTTCCGAAGGACAAGGATGAACTTGCCGCTCTCCAGGACAGACTAAGAAAGCGATTTCCAGTTGATGCATACAACAAAGCTCGAAGGGAGTTGGACCCTAACAGGATCCTTTCAAATAACAAGCTGGAAAAGCTTTTCCCGTTATCAGATACCATCTGA

Protein Analysis

225

Amino Acids

26.1

Weight (kDa)

5.43

Isoelectric Point (pI)

60.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALO PF04030 41 - 216 1.5e-11 D-arabinono-1,4-lactone oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029424)

Species Orthologous Gene IDs
pyrus_communis pycom12g07660
rosa_wichuraiana Rw0G012860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 295
Acc36I ACCTGC 2 cut(s) 289, 295
AccBSI CCGCTC 1 cut(s) 545
AciI CCGC 4 cut(s) 312, 412, 489, 543
AclWI GGATC 4 cut(s) 44, 401, 616, 629
AcsI RAATTY 1 cut(s) 144
AfaI GTAC 1 cut(s) 392
AfeI AGCGCT 1 cut(s) 305
AfiI CCNNNNNNNGG 3 cut(s) 91, 417, 421
AgsI TTSAA 3 cut(s) 236, 345, 630
AjnI CCWGG 1 cut(s) 549
AleI CACNNNNGTG 1 cut(s) 472
AluBI AGCT 7 cut(s) 10, 89, 259, 341, 596, 640, 649
AluI AGCT 7 cut(s) 10, 89, 259, 341, 596, 640, 649
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 2 cut(s) 179, 184
AlwI GGATC 4 cut(s) 44, 401, 616, 629
Aor51HI AGCGCT 1 cut(s) 305
ApeKI GCWGC 2 cut(s) 256, 301
ApoI RAATTY 1 cut(s) 144
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 309, 610
AvaII GGWCC 2 cut(s) 309, 610
BamHI GGATCC 1 cut(s) 621
BanII GRGCYC 2 cut(s) 12, 329
Bbv12I GWGCWC 1 cut(s) 12
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 2 cut(s) 268, 313
BccI CCATC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 551
BciVI GTATCC 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 179, 184
BfaI CTAG 1 cut(s) 47
BfmI CTRYAG 1 cut(s) 448
BfoI RGCGCY 1 cut(s) 307
BfuAI ACCTGC 2 cut(s) 289, 295
BfuI GTATCC 1 cut(s) 113
BisI GCNGC 3 cut(s) 257, 302, 543
BlsI GCNGC 3 cut(s) 258, 303, 544
Bme1390I CCNGG 1 cut(s) 551
Bme18I GGWCC 2 cut(s) 309, 610
BmgT120I GGNCC 2 cut(s) 309, 610
BmiI GGNNCC 4 cut(s) 205, 519, 612, 623
BmrFI CCNGG 1 cut(s) 551
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 1 cut(s) 571
BmuI ACTGGG 1 cut(s) 511
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 184
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 417, 421
Bse1I ACTGG 2 cut(s) 506, 575
BseBI CCWGG 1 cut(s) 551
BseGI GGATG 3 cut(s) 137, 364, 538
BseLI CCNNNNNNNGG 3 cut(s) 91, 417, 421
BseMII CTCAG 4 cut(s) 12, 81, 87, 195
BseNI ACTGG 2 cut(s) 506, 575
BseXI GCAGC 2 cut(s) 268, 313
BsgI GTGCAG 2 cut(s) 267, 301
BsiHKAI GWGCWC 1 cut(s) 12
BslI CCNNNNNNNGG 3 cut(s) 91, 417, 421
BsmAI GTCTC 2 cut(s) 179, 184
Bso31I GGTCTC 1 cut(s) 184
Bsp1286I GDGCHC 2 cut(s) 12, 329
Bsp143I GATC 4 cut(s) 49, 64, 406, 621
BspACI CCGC 4 cut(s) 312, 412, 489, 543
BspCNI CTCAG 4 cut(s) 13, 82, 88, 194
BspLI GGNNCC 4 cut(s) 205, 519, 612, 623
BspMI ACCTGC 2 cut(s) 289, 295
BspPI GGATC 4 cut(s) 44, 401, 616, 629
BspTNI GGTCTC 1 cut(s) 184
BsrBI CCGCTC 1 cut(s) 545
BsrI ACTGG 2 cut(s) 506, 575
BssMI GATC 4 cut(s) 49, 64, 406, 621
Bst2UI CCWGG 1 cut(s) 551
Bst4CI ACNGT 1 cut(s) 174
Bst6I CTCTTC 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 7 cut(s) 21, 90, 96, 181, 321, 507, 560
BstF5I GGATG 3 cut(s) 137, 364, 538
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 4 cut(s) 52, 67, 409, 624
BstMAI GTCTC 2 cut(s) 179, 184
BstMBI GATC 4 cut(s) 49, 64, 406, 621
BstMWI GCNNNNNNNGC 2 cut(s) 324, 646
BstNI CCWGG 1 cut(s) 551
BstSCI CCNGG 1 cut(s) 549
BstSFI CTRYAG 1 cut(s) 448
BstV1I GCAGC 2 cut(s) 268, 313
BstX2I RGATCY 3 cut(s) 49, 406, 621
BstYI RGATCY 3 cut(s) 49, 406, 621
BsuI GTATCC 1 cut(s) 113
BtsCI GGATG 3 cut(s) 137, 364, 538
BtsIMutI CAGTG 2 cut(s) 179, 499
BveI ACCTGC 2 cut(s) 289, 295
Cac8I GCNNGC 1 cut(s) 316
CfoI GCGC 1 cut(s) 306
Cfr13I GGNCC 2 cut(s) 309, 610
Csp6I GTAC 1 cut(s) 391
CviAII CATG 4 cut(s) 223, 331, 372, 388
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 7 cut(s) 21, 90, 96, 181, 321, 507, 560
DpnI GATC 4 cut(s) 51, 66, 408, 623
DpnII GATC 4 cut(s) 49, 64, 406, 621
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Ecl136II GAGCTC 1 cut(s) 10
Eco24I GRGCYC 2 cut(s) 12, 329
Eco31I GGTCTC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 309, 610
Eco47III AGCGCT 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 10
EcoICRI GAGCTC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 549
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 2 cut(s) 12, 329
FaeI CATG 4 cut(s) 226, 334, 375, 391
FaiI YATR 9 cut(s) 224, 242, 332, 365, 373, 383, 389, 495, 586
FatI CATG 4 cut(s) 222, 330, 371, 387
FauI CCCGC 1 cut(s) 419
Fnu4HI GCNGC 3 cut(s) 257, 302, 543
FokI GGATG 3 cut(s) 144, 371, 545
FriOI GRGCYC 2 cut(s) 12, 329
Fsp4HI GCNGC 3 cut(s) 257, 302, 543
FspBI CTAG 1 cut(s) 47
GlaI GCGC 1 cut(s) 305
GluI GCNGC 3 cut(s) 257, 302, 543
GsuI CTGGAG 1 cut(s) 533
HaeII RGCGCY 1 cut(s) 307
HhaI GCGC 1 cut(s) 306
Hin1II CATG 4 cut(s) 226, 334, 375, 391
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 2 cut(s) 82, 170
HindII GTYRAC 2 cut(s) 82, 170
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 1 cut(s) 334
HpaI GTTAAC 1 cut(s) 82
Hpy166II GTNNAC 2 cut(s) 82, 170
Hpy188I TCNGA 5 cut(s) 115, 184, 522, 664, 674
Hpy188III TCNNGA 3 cut(s) 56, 103, 265
Hpy8I GTNNAC 2 cut(s) 82, 170
HpyAV CCTTC 5 cut(s) 239, 404, 415, 517, 594
HpyCH4III ACNGT 1 cut(s) 174
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 4 cut(s) 74, 284, 318, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 324, 646
HpyF3I CTNAG 7 cut(s) 21, 90, 96, 181, 321, 507, 560
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 4 cut(s) 226, 334, 375, 391
HspAI GCGC 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 82
Kzo9I GATC 4 cut(s) 49, 64, 406, 621
LmnI GCTCC 1 cut(s) 295
Lsp1109I GCAGC 2 cut(s) 268, 313
LweI GCATC 1 cut(s) 571
MaeI CTAG 1 cut(s) 47
MaeII ACGT 1 cut(s) 69
MalI GATC 4 cut(s) 51, 66, 408, 623
MbiI CCGCTC 1 cut(s) 545
MboI GATC 4 cut(s) 49, 64, 406, 621
MboII GAAGA 3 cut(s) 118, 280, 446
MfeI CAATTG 1 cut(s) 470
MflI RGATCY 3 cut(s) 49, 406, 621
MhlI GDGCHC 2 cut(s) 12, 329
MluCI AATT 3 cut(s) 144, 426, 470
MlyI GAGTC 1 cut(s) 343
MmeI TCCRAC 2 cut(s) 93, 588
MnlI CCTC 5 cut(s) 63, 85, 109, 349, 508
Mph1103I ATGCAT 1 cut(s) 586
MseI TTAA 2 cut(s) 26, 81
MslI CAYNNNNRTG 1 cut(s) 472
MspR9I CCNGG 1 cut(s) 551
MunI CAATTG 1 cut(s) 470
MvaI CCWGG 1 cut(s) 551
MwoI GCNNNNNNNGC 2 cut(s) 324, 646
NdeII GATC 4 cut(s) 49, 64, 406, 621
NlaIII CATG 4 cut(s) 226, 334, 375, 391
NlaIV GGNNCC 4 cut(s) 205, 519, 612, 623
NsiI ATGCAT 1 cut(s) 586
OliI CACNNNNGTG 1 cut(s) 472
PaqCI CACCTGC 1 cut(s) 295
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 3 cut(s) 258, 303, 544
PleI GAGTC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 549
PspGI CCWGG 1 cut(s) 549
PspN4I GGNNCC 4 cut(s) 205, 519, 612, 623
PspPI GGNCC 2 cut(s) 309, 610
PsuI RGATCY 3 cut(s) 49, 406, 621
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 472
SacI GAGCTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 26, 81
SatI GCNGC 3 cut(s) 257, 302, 543
Sau3AI GATC 4 cut(s) 49, 64, 406, 621
Sau96I GGNCC 2 cut(s) 309, 610
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 1 cut(s) 551
SduI GDGCHC 2 cut(s) 12, 329
SfaNI GCATC 1 cut(s) 571
SfcI CTRYAG 1 cut(s) 448
SinI GGWCC 2 cut(s) 309, 610
SmiMI CAYNNNNRTG 1 cut(s) 472
Sse9I AATT 3 cut(s) 144, 426, 470
SsiI CCGC 4 cut(s) 312, 412, 489, 543
SspMI CTAG 1 cut(s) 47
SstI GAGCTC 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 549
TaaI ACNGT 1 cut(s) 174
TaiI ACGT 1 cut(s) 72
TaqI TCGA 3 cut(s) 57, 264, 598
TasI AATT 3 cut(s) 144, 426, 470
TauI GCSGC 1 cut(s) 545
Tru1I TTAA 2 cut(s) 26, 81
Tru9I TTAA 2 cut(s) 26, 81
TscAI CASTG 2 cut(s) 179, 506
TseI GCWGC 2 cut(s) 256, 301
TspDTI ATGAA 6 cut(s) 17, 156, 239, 360, 510, 549
TspRI CASTG 2 cut(s) 179, 506
VpaK11BI GGWCC 2 cut(s) 309, 610
XapI RAATTY 1 cut(s) 144
XspI CTAG 1 cut(s) 47
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.