Rw0G012860

L-galactono-1,4-lactone dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00571
OG0029424
Physical Location & Seq
Forward (+)
1332 .. 2638
1307 bp
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UTR
Exon/CDS
Intron
Rw0G012860.1

Sequence Viewer

Length: 453 bp
ATGAAAGACCTTGAATACATAGCAGATCTAAAACAGCTAATAGAGAAAGAAGAGATACCTGCACCTGCTCCAATAGAGCAGCGCTGGACAGCCAGCACTAAGAGCCCCATGAGTCCAGCTTCAAGCTCAAGAGAGGATGATATATTTTCATGGGTTGGCATAATCATGTACCTTCCAACAACAGATGCCCGCCAAAGAAAGGATATTACAGAAGAGTTTTTCCACTACAGGCATCTAACCCAGACACGGTTGTGGGATACGTATTCTTCTTATGAACACTGGGCCAAGATTGAGGTCCCAAAGGACAAAGAACAGCTTACCGCTCTGCAGGCAAGGCTAAGGAAGCGTTTTCCAGTTGACGCCTACAACAAAGCACGAACAGAATTAGACCCAAACCGGATCCTTTCAAACATCAAGCTGGAAAAGCTTTTCCCATTGTCTGATACCATTTGA

Protein Analysis

150

Amino Acids

17.71

Weight (kDa)

6.32

Isoelectric Point (pI)

68.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALO PF04030 5 - 140 6e-10 D-arabinono-1,4-lactone oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029424)

Species Orthologous Gene IDs
pyrus_communis pycom12g07660
rosa_wichuraiana Rw0G012860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 2 cut(s) 67, 73
AccBSI CCGCTC 1 cut(s) 323
AciI CCGC 2 cut(s) 190, 321
AclWI GGATC 2 cut(s) 394, 407
AcyI GRCGYC 1 cut(s) 360
AfaI GTAC 1 cut(s) 170
AfeI AGCGCT 1 cut(s) 83
AfiI CCNNNNNNNGG 2 cut(s) 199, 246
AgsI TTSAA 3 cut(s) 14, 123, 408
AleI CACNNNNGTG 1 cut(s) 250
AluBI AGCT 6 cut(s) 37, 119, 126, 316, 418, 427
AluI AGCT 6 cut(s) 37, 119, 126, 316, 418, 427
AlwI GGATC 2 cut(s) 394, 407
Aor51HI AGCGCT 1 cut(s) 83
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 1 cut(s) 79
AspLEI GCGC 1 cut(s) 84
AspS9I GGNCC 2 cut(s) 282, 295
AvaII GGWCC 1 cut(s) 295
BamHI GGATCC 1 cut(s) 399
BanII GRGCYC 1 cut(s) 107
BbvI GCAGC 1 cut(s) 91
BciVI GTATCC 1 cut(s) 250
BfmI CTRYAG 2 cut(s) 226, 326
BfoI RGCGCY 1 cut(s) 85
BfuAI ACCTGC 2 cut(s) 67, 73
BfuI GTATCC 1 cut(s) 250
BglII AGATCT 1 cut(s) 25
BisI GCNGC 1 cut(s) 80
BlsI GCNGC 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 2 cut(s) 282, 295
BmiI GGNNCC 2 cut(s) 297, 401
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 2 cut(s) 175, 241
BmuI ACTGGG 1 cut(s) 289
Bpu10I CCTNAGC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 112
BsaAI YACGTR 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 360
BsaWI WCCGGW 1 cut(s) 396
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 246
Bse1I ACTGG 2 cut(s) 284, 353
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 2 cut(s) 199, 246
BseNI ACTGG 2 cut(s) 284, 353
BseXI GCAGC 1 cut(s) 91
BsgI GTGCAG 1 cut(s) 45
BshFI GGCC 1 cut(s) 284
BsiSI CCGG 1 cut(s) 397
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 2 cut(s) 199, 246
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 1 cut(s) 284
Bsp1286I GDGCHC 1 cut(s) 107
Bsp143I GATC 2 cut(s) 25, 399
BspACI CCGC 2 cut(s) 190, 321
BspANI GGCC 1 cut(s) 284
BspLI GGNNCC 2 cut(s) 297, 401
BspMAI CTGCAG 1 cut(s) 330
BspMI ACCTGC 2 cut(s) 67, 73
BspPI GGATC 2 cut(s) 394, 407
BsrBI CCGCTC 1 cut(s) 323
BsrI ACTGG 2 cut(s) 284, 353
BssMI GATC 2 cut(s) 25, 399
BssNI GRCGYC 1 cut(s) 360
Bst4CI ACNGT 1 cut(s) 249
Bst6I CTCTTC 2 cut(s) 45, 207
BstACI GRCGYC 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 261
BstC8I GCNNGC 3 cut(s) 94, 190, 330
BstDEI CTNAG 2 cut(s) 99, 338
BstF5I GGATG 1 cut(s) 142
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 1 cut(s) 84
BstKTI GATC 2 cut(s) 28, 402
BstMBI GATC 2 cut(s) 25, 399
BstMWI GCNNNNNNNGC 5 cut(s) 102, 329, 334, 343, 424
BstSFI CTRYAG 2 cut(s) 226, 326
BstSNI TACGTA 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 91
BstX2I RGATCY 2 cut(s) 25, 399
BstYI RGATCY 2 cut(s) 25, 399
BsuI GTATCC 1 cut(s) 250
BsuRI GGCC 1 cut(s) 284
BtsCI GGATG 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 277
BveI ACCTGC 2 cut(s) 67, 73
Cac8I GCNNGC 3 cut(s) 94, 190, 330
CfoI GCGC 1 cut(s) 84
Cfr13I GGNCC 2 cut(s) 282, 295
CseI GACGC 1 cut(s) 368
Csp6I GTAC 1 cut(s) 169
CviAII CATG 3 cut(s) 109, 150, 166
CviQI GTAC 1 cut(s) 169
DdeI CTNAG 2 cut(s) 99, 338
DpnI GATC 2 cut(s) 27, 401
DpnII GATC 2 cut(s) 25, 399
Eam1104I CTCTTC 2 cut(s) 45, 207
EarI CTCTTC 2 cut(s) 45, 207
Eco105I TACGTA 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 295
Eco47III AGCGCT 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 107
FaeI CATG 3 cut(s) 112, 153, 169
FaiI YATR 7 cut(s) 20, 110, 143, 151, 161, 167, 273
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 1 cut(s) 281
FatI CATG 3 cut(s) 108, 149, 165
FauI CCCGC 1 cut(s) 197
Fnu4HI GCNGC 1 cut(s) 80
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 1 cut(s) 80
GlaI GCGC 1 cut(s) 83
GluI GCNGC 1 cut(s) 80
HaeII RGCGCY 1 cut(s) 85
HaeIII GGCC 1 cut(s) 284
HapII CCGG 1 cut(s) 397
HgaI GACGC 1 cut(s) 368
HhaI GCGC 1 cut(s) 84
Hin1I GRCGYC 1 cut(s) 360
Hin1II CATG 3 cut(s) 112, 153, 169
Hin6I GCGC 1 cut(s) 82
HinP1I GCGC 1 cut(s) 82
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 1 cut(s) 112
HpaII CCGG 1 cut(s) 397
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 1 cut(s) 129
Hpy8I GTNNAC 1 cut(s) 358
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 1 cut(s) 249
HpyCH4IV ACGT 1 cut(s) 260
HpyCH4V TGCA 2 cut(s) 62, 328
HpyF10VI GCNNNNNNNGC 5 cut(s) 102, 329, 334, 343, 424
HpyF3I CTNAG 2 cut(s) 99, 338
HpySE526I ACGT 1 cut(s) 260
Hsp92I GRCGYC 1 cut(s) 360
Hsp92II CATG 3 cut(s) 112, 153, 169
HspAI GCGC 1 cut(s) 82
Kzo9I GATC 2 cut(s) 25, 399
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 2 cut(s) 175, 241
MaeII ACGT 1 cut(s) 260
MalI GATC 2 cut(s) 27, 401
MbiI CCGCTC 1 cut(s) 323
MboI GATC 2 cut(s) 25, 399
MboII GAAGA 3 cut(s) 62, 224, 258
MflI RGATCY 2 cut(s) 25, 399
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 1 cut(s) 383
MlyI GAGTC 1 cut(s) 121
MmeI TCCRAC 1 cut(s) 200
MnlI CCTC 2 cut(s) 127, 286
MslI CAYNNNNRTG 2 cut(s) 164, 250
MspI CCGG 1 cut(s) 397
MwoI GCNNNNNNNGC 5 cut(s) 102, 329, 334, 343, 424
NdeII GATC 2 cut(s) 25, 399
NlaIII CATG 3 cut(s) 112, 153, 169
NlaIV GGNNCC 2 cut(s) 297, 401
OliI CACNNNNGTG 1 cut(s) 250
PaqCI CACCTGC 1 cut(s) 73
PkrI GCNGC 1 cut(s) 81
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
Ppu21I YACGTR 1 cut(s) 261
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
PspN4I GGNNCC 2 cut(s) 297, 401
PspPI GGNCC 2 cut(s) 282, 295
PspPPI RGGWCCY 1 cut(s) 295
PstI CTGCAG 1 cut(s) 330
PsuI RGATCY 2 cut(s) 25, 399
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
RseI CAYNNNNRTG 2 cut(s) 164, 250
SatI GCNGC 1 cut(s) 80
Sau3AI GATC 2 cut(s) 25, 399
Sau96I GGNCC 2 cut(s) 282, 295
SchI GAGTC 1 cut(s) 121
SduI GDGCHC 1 cut(s) 107
SfaNI GCATC 2 cut(s) 175, 241
SfcI CTRYAG 2 cut(s) 226, 326
SinI GGWCC 1 cut(s) 295
SmiMI CAYNNNNRTG 2 cut(s) 164, 250
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
SnaBI TACGTA 1 cut(s) 261
Sse9I AATT 1 cut(s) 383
SsiI CCGC 2 cut(s) 190, 321
TaaI ACNGT 1 cut(s) 249
TaiI ACGT 1 cut(s) 263
TasI AATT 1 cut(s) 383
TscAI CASTG 1 cut(s) 284
TseI GCWGC 1 cut(s) 79
TspDTI ATGAA 3 cut(s) 17, 138, 288
TspRI CASTG 1 cut(s) 284
VpaK11BI GGWCC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.