pycom12g07660

L-galactono-1,4-lactone dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0029424
Physical Location & Seq
Reverse (-)
8234509 .. 8236687
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGAATGAGCTCTCATTTACTGAGTTAAGAGATAAACTACTTGCCCTAGATCCTCTCGACAAAGATCACGTTGCAAAGGTTAACCAAGCTGAGGCTGAGTTCTGGAAGAAGTCGGAGGGATACAGAGTGGGATGGAGTGATGAAATTTTGGGCTTTGATTGTGGTGGTCAACAGTGGGTCTCAGAGACTTGTTTTCCAGCAGGAACCCTTGCCAAACCAAGCATGAAAGACCTTGAATACATAGAAGGTGTAAAGCAGCTCATCGAGAAGAATGAGATACCTGCACCTGCTCCAATAGAGCAGCGCTGGACCGCCTGCACTAAGAGCCCCATGAGTCCAGCTTCAAGCACAAGAGAGGATGATATATTTTCATGGGTTGGTATAATCATGTACCTTCCTACAACAGATCCCCGCCAAAGGAAGGAAATTACAGAAGAGTTTTTTCACTACAGGCGACTGACCCAGACACAATTGTGGGATAAGTATTCCGCTTATGAACACTGGGCTAAGATTGAGGTTCCGAAGGACAAGGATGAACTTGCCGCTCTCCAGGACAGACTAAGAAAGCGATTTCCAGTTGATGCATACAACAAAGCTCGAAGGGAGTTGGACCCTAACAGGATCCTTTCAAATAACAAGCTGGAAAAGCTTTTCCCGTTATCAGATACCATCTGA

Protein Analysis

225

Amino Acids

26.1

Weight (kDa)

5.43

Isoelectric Point (pI)

60.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALO PF04030 41 - 216 1.5e-11 D-arabinono-1,4-lactone oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029424)

Species Orthologous Gene IDs
pyrus_communis pycom12g07660
rosa_wichuraiana Rw0G012860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 295
Acc36I ACCTGC 2 cut(s) 289, 295
AccBSI CCGCTC 1 cut(s) 545
AciI CCGC 4 cut(s) 312, 412, 489, 543
AclWI GGATC 4 cut(s) 44, 401, 616, 629
AcsI RAATTY 1 cut(s) 144
AfaI GTAC 1 cut(s) 392
AfeI AGCGCT 1 cut(s) 305
AfiI CCNNNNNNNGG 3 cut(s) 91, 417, 421
AgsI TTSAA 3 cut(s) 236, 345, 630
AjnI CCWGG 1 cut(s) 549
AleI CACNNNNGTG 1 cut(s) 472
AluBI AGCT 7 cut(s) 10, 89, 259, 341, 596, 640, 649
AluI AGCT 7 cut(s) 10, 89, 259, 341, 596, 640, 649
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 2 cut(s) 179, 184
AlwI GGATC 4 cut(s) 44, 401, 616, 629
Aor51HI AGCGCT 1 cut(s) 305
ApeKI GCWGC 2 cut(s) 256, 301
ApoI RAATTY 1 cut(s) 144
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 309, 610
AvaII GGWCC 2 cut(s) 309, 610
BamHI GGATCC 1 cut(s) 621
BanII GRGCYC 2 cut(s) 12, 329
Bbv12I GWGCWC 1 cut(s) 12
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 2 cut(s) 268, 313
BccI CCATC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 551
BciVI GTATCC 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 179, 184
BfaI CTAG 1 cut(s) 47
BfmI CTRYAG 1 cut(s) 448
BfoI RGCGCY 1 cut(s) 307
BfuAI ACCTGC 2 cut(s) 289, 295
BfuI GTATCC 1 cut(s) 113
BisI GCNGC 3 cut(s) 257, 302, 543
BlsI GCNGC 3 cut(s) 258, 303, 544
Bme1390I CCNGG 1 cut(s) 551
Bme18I GGWCC 2 cut(s) 309, 610
BmgT120I GGNCC 2 cut(s) 309, 610
BmiI GGNNCC 4 cut(s) 205, 519, 612, 623
BmrFI CCNGG 1 cut(s) 551
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 1 cut(s) 571
BmuI ACTGGG 1 cut(s) 511
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 184
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 417, 421
Bse1I ACTGG 2 cut(s) 506, 575
BseBI CCWGG 1 cut(s) 551
BseGI GGATG 3 cut(s) 137, 364, 538
BseLI CCNNNNNNNGG 3 cut(s) 91, 417, 421
BseMII CTCAG 4 cut(s) 12, 81, 87, 195
BseNI ACTGG 2 cut(s) 506, 575
BseXI GCAGC 2 cut(s) 268, 313
BsgI GTGCAG 2 cut(s) 267, 301
BsiHKAI GWGCWC 1 cut(s) 12
BslI CCNNNNNNNGG 3 cut(s) 91, 417, 421
BsmAI GTCTC 2 cut(s) 179, 184
Bso31I GGTCTC 1 cut(s) 184
Bsp1286I GDGCHC 2 cut(s) 12, 329
Bsp143I GATC 4 cut(s) 49, 64, 406, 621
BspACI CCGC 4 cut(s) 312, 412, 489, 543
BspCNI CTCAG 4 cut(s) 13, 82, 88, 194
BspLI GGNNCC 4 cut(s) 205, 519, 612, 623
BspMI ACCTGC 2 cut(s) 289, 295
BspPI GGATC 4 cut(s) 44, 401, 616, 629
BspTNI GGTCTC 1 cut(s) 184
BsrBI CCGCTC 1 cut(s) 545
BsrI ACTGG 2 cut(s) 506, 575
BssMI GATC 4 cut(s) 49, 64, 406, 621
Bst2UI CCWGG 1 cut(s) 551
Bst4CI ACNGT 1 cut(s) 174
Bst6I CTCTTC 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 7 cut(s) 21, 90, 96, 181, 321, 507, 560
BstF5I GGATG 3 cut(s) 137, 364, 538
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 4 cut(s) 52, 67, 409, 624
BstMAI GTCTC 2 cut(s) 179, 184
BstMBI GATC 4 cut(s) 49, 64, 406, 621
BstMWI GCNNNNNNNGC 2 cut(s) 324, 646
BstNI CCWGG 1 cut(s) 551
BstSCI CCNGG 1 cut(s) 549
BstSFI CTRYAG 1 cut(s) 448
BstV1I GCAGC 2 cut(s) 268, 313
BstX2I RGATCY 3 cut(s) 49, 406, 621
BstYI RGATCY 3 cut(s) 49, 406, 621
BsuI GTATCC 1 cut(s) 113
BtsCI GGATG 3 cut(s) 137, 364, 538
BtsIMutI CAGTG 2 cut(s) 179, 499
BveI ACCTGC 2 cut(s) 289, 295
Cac8I GCNNGC 1 cut(s) 316
CfoI GCGC 1 cut(s) 306
Cfr13I GGNCC 2 cut(s) 309, 610
Csp6I GTAC 1 cut(s) 391
CviAII CATG 4 cut(s) 223, 331, 372, 388
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 7 cut(s) 21, 90, 96, 181, 321, 507, 560
DpnI GATC 4 cut(s) 51, 66, 408, 623
DpnII GATC 4 cut(s) 49, 64, 406, 621
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Ecl136II GAGCTC 1 cut(s) 10
Eco24I GRGCYC 2 cut(s) 12, 329
Eco31I GGTCTC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 309, 610
Eco47III AGCGCT 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 10
EcoICRI GAGCTC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 549
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 2 cut(s) 12, 329
FaeI CATG 4 cut(s) 226, 334, 375, 391
FaiI YATR 9 cut(s) 224, 242, 332, 365, 373, 383, 389, 495, 586
FatI CATG 4 cut(s) 222, 330, 371, 387
FauI CCCGC 1 cut(s) 419
Fnu4HI GCNGC 3 cut(s) 257, 302, 543
FokI GGATG 3 cut(s) 144, 371, 545
FriOI GRGCYC 2 cut(s) 12, 329
Fsp4HI GCNGC 3 cut(s) 257, 302, 543
FspBI CTAG 1 cut(s) 47
GlaI GCGC 1 cut(s) 305
GluI GCNGC 3 cut(s) 257, 302, 543
GsuI CTGGAG 1 cut(s) 533
HaeII RGCGCY 1 cut(s) 307
HhaI GCGC 1 cut(s) 306
Hin1II CATG 4 cut(s) 226, 334, 375, 391
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 2 cut(s) 82, 170
HindII GTYRAC 2 cut(s) 82, 170
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 1 cut(s) 334
HpaI GTTAAC 1 cut(s) 82
Hpy166II GTNNAC 2 cut(s) 82, 170
Hpy188I TCNGA 5 cut(s) 115, 184, 522, 664, 674
Hpy188III TCNNGA 3 cut(s) 56, 103, 265
Hpy8I GTNNAC 2 cut(s) 82, 170
HpyAV CCTTC 5 cut(s) 239, 404, 415, 517, 594
HpyCH4III ACNGT 1 cut(s) 174
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 4 cut(s) 74, 284, 318, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 324, 646
HpyF3I CTNAG 7 cut(s) 21, 90, 96, 181, 321, 507, 560
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 4 cut(s) 226, 334, 375, 391
HspAI GCGC 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 82
Kzo9I GATC 4 cut(s) 49, 64, 406, 621
LmnI GCTCC 1 cut(s) 295
Lsp1109I GCAGC 2 cut(s) 268, 313
LweI GCATC 1 cut(s) 571
MaeI CTAG 1 cut(s) 47
MaeII ACGT 1 cut(s) 69
MalI GATC 4 cut(s) 51, 66, 408, 623
MbiI CCGCTC 1 cut(s) 545
MboI GATC 4 cut(s) 49, 64, 406, 621
MboII GAAGA 3 cut(s) 118, 280, 446
MfeI CAATTG 1 cut(s) 470
MflI RGATCY 3 cut(s) 49, 406, 621
MhlI GDGCHC 2 cut(s) 12, 329
MluCI AATT 3 cut(s) 144, 426, 470
MlyI GAGTC 1 cut(s) 343
MmeI TCCRAC 2 cut(s) 93, 588
MnlI CCTC 5 cut(s) 63, 85, 109, 349, 508
Mph1103I ATGCAT 1 cut(s) 586
MseI TTAA 2 cut(s) 26, 81
MslI CAYNNNNRTG 1 cut(s) 472
MspR9I CCNGG 1 cut(s) 551
MunI CAATTG 1 cut(s) 470
MvaI CCWGG 1 cut(s) 551
MwoI GCNNNNNNNGC 2 cut(s) 324, 646
NdeII GATC 4 cut(s) 49, 64, 406, 621
NlaIII CATG 4 cut(s) 226, 334, 375, 391
NlaIV GGNNCC 4 cut(s) 205, 519, 612, 623
NsiI ATGCAT 1 cut(s) 586
OliI CACNNNNGTG 1 cut(s) 472
PaqCI CACCTGC 1 cut(s) 295
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 3 cut(s) 258, 303, 544
PleI GAGTC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 549
PspGI CCWGG 1 cut(s) 549
PspN4I GGNNCC 4 cut(s) 205, 519, 612, 623
PspPI GGNCC 2 cut(s) 309, 610
PsuI RGATCY 3 cut(s) 49, 406, 621
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 472
SacI GAGCTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 26, 81
SatI GCNGC 3 cut(s) 257, 302, 543
Sau3AI GATC 4 cut(s) 49, 64, 406, 621
Sau96I GGNCC 2 cut(s) 309, 610
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 1 cut(s) 551
SduI GDGCHC 2 cut(s) 12, 329
SfaNI GCATC 1 cut(s) 571
SfcI CTRYAG 1 cut(s) 448
SinI GGWCC 2 cut(s) 309, 610
SmiMI CAYNNNNRTG 1 cut(s) 472
Sse9I AATT 3 cut(s) 144, 426, 470
SsiI CCGC 4 cut(s) 312, 412, 489, 543
SspMI CTAG 1 cut(s) 47
SstI GAGCTC 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 549
TaaI ACNGT 1 cut(s) 174
TaiI ACGT 1 cut(s) 72
TaqI TCGA 3 cut(s) 57, 264, 598
TasI AATT 3 cut(s) 144, 426, 470
TauI GCSGC 1 cut(s) 545
Tru1I TTAA 2 cut(s) 26, 81
Tru9I TTAA 2 cut(s) 26, 81
TscAI CASTG 2 cut(s) 179, 506
TseI GCWGC 2 cut(s) 256, 301
TspDTI ATGAA 6 cut(s) 17, 156, 239, 360, 510, 549
TspRI CASTG 2 cut(s) 179, 506
VpaK11BI GGWCC 2 cut(s) 309, 610
XapI RAATTY 1 cut(s) 144
XspI CTAG 1 cut(s) 47
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.