pycom12g07750

Belongs to the pyrroline-5-carboxylate reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0024418
Physical Location & Seq
Reverse (-)
8408037 .. 8412163
4127 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 843 bp
ATGGCAGGAGGTGAGGAAGTGGAAGTCAAACCAATTGACAAGGAAGCCTTCAATTTGGGGTTCATTGGAGCTGGTAAATTGGCAGCAAGTGTTGCCATCGGATTGGTCAACCAGGGTATTGTACCACCTTCTCGAATTTTCACGGCTCACCGGAATCCAGAAAGCCGGCTGGATTTTACATCTGTTGGAATTCAGGTTTATCAGAACAATCATCAGGTGGCTGAAGAGTGTGATGTAATCATTTTCTCAGTGAAACCTCAAATACTTAAGGAAGTAATCCTGGACTTACGGCCTCTACTTTCACAAAATAAACTTCTTGTGTCGCTTGCTGTGGGACACAAACTGAAAGCTCTACAGGAATGGGCTGGTCATTGCCGATTTGTTAGAGTAATCCCTAATACCGCTTCTGTCATTGGTGAAGCAGCTACAGTTATGCACTTGGGTGAAGCTGCAACAGTAGACGACGGTGAGTTAATAGCAGACCTATTTGGAGCTGTTGGCAAGGTGTGGAAAATGGACGAGAAATTGTCTGATGCTGCTGGTGCTGTTGGTGGCGCTGGTACAGCATTTGCATCTATAGCAATAGAAGCTATGGCTGATGGAGGTGTAGCTGCAGGCTTACCACGAGAAATTTCTCTGAGTCTTGCTTCTCAAGCTTTCTATGGAGCAGCAGCCATGGTGATGAAAACAGGAAAACATCCAGGTCAGCTGAAAGACCAAGTTGCATCACCTGGAGGAATCACCATTGCCGGGATTCATGAGCTTGAGAAGGGCGCATTTCGTGCAAGCATGATGAACGCGGTTGTTGCAGCTGCTAAGCGTAGCCATGAGATGGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01130 ko01230 map00330 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
F420_oxidored P5CR_dimer

Protein Analysis

281

Amino Acids

29.24

Weight (kDa)

6.07

Isoelectric Point (pI)

26.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 19 - 112 5.4e-16 NADP oxidoreductase coenzyme F420-dependent
P5CR_dimer PF14748 174 - 278 2.5e-33 Pyrroline-5-carboxylate reductase dimerisation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024418)

Species Orthologous Gene IDs
malus_domestica MD12G1091800.v1.1
prunus_persica Prupe.7G045400_v2.0.a1
pyrus_communis pycom12g07750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 832
AccI GTMKAC 1 cut(s) 459
AccII CGCG 1 cut(s) 800
AciI CCGC 2 cut(s) 402, 800
AcsI RAATTY 3 cut(s) 135, 189, 630
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 2 cut(s) 123, 562
AfiI CCNNNNNNNGG 2 cut(s) 750, 832
AflII CTTAAG 1 cut(s) 266
AgsI TTSAA 1 cut(s) 52
AjnI CCWGG 4 cut(s) 111, 279, 700, 730
AleI CACNNNNGTG 1 cut(s) 441
AoxI GGCC 1 cut(s) 290
ApeKI GCWGC 9 cut(s) 83, 422, 449, 536, 611, 668, 671, 809, 812
ApoI RAATTY 3 cut(s) 135, 189, 630
AspLEI GCGC 2 cut(s) 557, 776
AsuC2I CCSGG 1 cut(s) 751
AsuHPI GGTGA 8 cut(s) 23, 140, 428, 455, 479, 691, 720, 733
BauI CACGAG 1 cut(s) 624
BbvI GCAGC 9 cut(s) 95, 434, 436, 523, 598, 680, 683, 799, 821
BccI CCATC 3 cut(s) 104, 593, 826
BceAI ACGGC 2 cut(s) 159, 305
BciT130I CCWGG 4 cut(s) 113, 281, 702, 732
BcnI CCSGG 1 cut(s) 751
BfmI CTRYAG 4 cut(s) 353, 426, 576, 612
BfoI RGCGCY 1 cut(s) 558
BfrI CTTAAG 1 cut(s) 266
BisI GCNGC 9 cut(s) 84, 423, 450, 537, 612, 669, 672, 810, 813
BlpI GCTNAGC 1 cut(s) 816
BlsI GCNGC 9 cut(s) 85, 424, 451, 538, 613, 670, 673, 811, 814
Bme1390I CCNGG 5 cut(s) 113, 281, 702, 732, 751
BmrFI CCNGG 5 cut(s) 113, 281, 702, 732, 751
BmsI GCATC 3 cut(s) 523, 581, 734
BpmI CTGGAG 1 cut(s) 753
Bpu1102I GCTNAGC 1 cut(s) 816
BpuEI CTTGAG 2 cut(s) 636, 785
BpuMI CCSGG 1 cut(s) 751
BsaJI CCNNGG 2 cut(s) 112, 675
BsaWI WCCGGW 1 cut(s) 150
Bsc4I CCNNNNNNNGG 2 cut(s) 750, 832
Bse118I RCCGGY 1 cut(s) 165
Bse3DI GCAATG 2 cut(s) 370, 744
BseBI CCWGG 4 cut(s) 113, 281, 702, 732
BseDI CCNNGG 2 cut(s) 112, 675
BseGI GGATG 1 cut(s) 697
BseLI CCNNNNNNNGG 2 cut(s) 750, 832
BseMI GCAATG 2 cut(s) 370, 744
BseMII CTCAG 2 cut(s) 261, 629
BseXI GCAGC 9 cut(s) 95, 434, 436, 523, 598, 680, 683, 799, 821
Bsh1236I CGCG 1 cut(s) 800
BshFI GGCC 1 cut(s) 292
BsiSI CCGG 3 cut(s) 151, 166, 750
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 750, 832
BsmFI GGGAC 1 cut(s) 348
BsnI GGCC 1 cut(s) 292
Bsp1720I GCTNAGC 1 cut(s) 816
Bsp19I CCATGG 1 cut(s) 675
BspACI CCGC 2 cut(s) 402, 800
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 2 cut(s) 260, 630
BspFNI CGCG 1 cut(s) 800
BspHI TCATGA 1 cut(s) 757
BspMAI CTGCAG 1 cut(s) 616
BspTI CTTAAG 1 cut(s) 266
BsrDI GCAATG 2 cut(s) 370, 744
BsrFI RCCGGY 1 cut(s) 165
BssAI RCCGGY 1 cut(s) 165
BssECI CCNNGG 2 cut(s) 112, 675
BssSI CACGAG 1 cut(s) 624
BssT1I CCWWGG 1 cut(s) 675
Bst2BI CACGAG 1 cut(s) 624
Bst2UI CCWGG 4 cut(s) 113, 281, 702, 732
Bst4CI ACNGT 3 cut(s) 430, 457, 467
Bst6I CTCTTC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 266
BstAPI GCANNNNNTGC 2 cut(s) 92, 782
BstC8I GCNNGC 4 cut(s) 167, 327, 616, 787
BstDEI CTNAG 3 cut(s) 247, 638, 816
BstDSI CCRYGG 1 cut(s) 675
BstF5I GGATG 1 cut(s) 697
BstFNI CGCG 1 cut(s) 800
BstH2I RGCGCY 1 cut(s) 558
BstHHI GCGC 2 cut(s) 557, 776
BstMWI GCNNNNNNNGC 8 cut(s) 92, 542, 563, 578, 587, 653, 782, 806
BstNI CCWGG 4 cut(s) 113, 281, 702, 732
BstSCI CCNGG 5 cut(s) 111, 279, 700, 730, 749
BstSFI CTRYAG 4 cut(s) 353, 426, 576, 612
BstUI CGCG 1 cut(s) 800
BstV1I GCAGC 9 cut(s) 95, 434, 436, 523, 598, 680, 683, 799, 821
BstXI CCANNNNNNTGG 1 cut(s) 103
BsuRI GGCC 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 675
BtsCI GGATG 1 cut(s) 697
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 4 cut(s) 167, 327, 616, 787
CciI TCATGA 1 cut(s) 757
CfoI GCGC 2 cut(s) 557, 776
Cfr10I RCCGGY 1 cut(s) 165
Csp6I GTAC 2 cut(s) 122, 561
CviAII CATG 4 cut(s) 676, 758, 790, 827
CviQI GTAC 2 cut(s) 122, 561
DdeI CTNAG 3 cut(s) 247, 638, 816
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 675
Eco57I CTGAAG 1 cut(s) 243
EcoRI GAATTC 1 cut(s) 189
EcoRII CCWGG 4 cut(s) 111, 279, 700, 730
EcoT14I CCWWGG 1 cut(s) 675
ErhI CCWWGG 1 cut(s) 675
FaeI CATG 4 cut(s) 679, 761, 793, 830
FaiI YATR 8 cut(s) 434, 578, 593, 663, 677, 759, 791, 828
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FaqI GGGAC 1 cut(s) 348
FatI CATG 4 cut(s) 675, 757, 789, 826
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 9 cut(s) 84, 423, 450, 537, 612, 669, 672, 810, 813
FokI GGATG 1 cut(s) 684
Fsp4HI GCNGC 9 cut(s) 84, 423, 450, 537, 612, 669, 672, 810, 813
GlaI GCGC 2 cut(s) 556, 775
GluI GCNGC 9 cut(s) 84, 423, 450, 537, 612, 669, 672, 810, 813
GsuI CTGGAG 1 cut(s) 753
HaeII RGCGCY 1 cut(s) 558
HaeIII GGCC 1 cut(s) 292
HapII CCGG 3 cut(s) 151, 166, 750
HhaI GCGC 2 cut(s) 557, 776
Hin1II CATG 4 cut(s) 679, 761, 793, 830
Hin6I GCGC 2 cut(s) 555, 774
HinP1I GCGC 2 cut(s) 555, 774
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 4 cut(s) 154, 640, 738, 754
HpaII CCGG 3 cut(s) 151, 166, 750
HphI GGTGA 8 cut(s) 23, 140, 428, 455, 479, 691, 720, 733
Hpy166II GTNNAC 2 cut(s) 109, 460
Hpy188I TCNGA 4 cut(s) 101, 204, 532, 639
Hpy188III TCNNGA 3 cut(s) 132, 158, 758
Hpy8I GTNNAC 2 cut(s) 109, 460
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 3 cut(s) 58, 138, 763
HpyCH4III ACNGT 3 cut(s) 430, 457, 467
HpyCH4V TGCA 7 cut(s) 436, 452, 572, 614, 725, 785, 809
HpyF10VI GCNNNNNNNGC 8 cut(s) 92, 542, 563, 578, 587, 653, 782, 806
HpyF3I CTNAG 3 cut(s) 247, 638, 816
Hsp92II CATG 4 cut(s) 679, 761, 793, 830
HspAI GCGC 2 cut(s) 555, 774
KroI GCCGGC 1 cut(s) 165
KroNI GCCGGC 1 cut(s) 167
LmnI GCTCC 3 cut(s) 68, 491, 665
Lsp1109I GCAGC 9 cut(s) 95, 434, 436, 523, 598, 680, 683, 799, 821
LweI GCATC 3 cut(s) 523, 581, 734
MboII GAAGA 1 cut(s) 236
MfeI CAATTG 1 cut(s) 33
MluCI AATT 7 cut(s) 33, 52, 77, 135, 189, 524, 630
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 5 cut(s) 7, 267, 303, 596, 728
MroNI GCCGGC 1 cut(s) 165
MseI TTAA 2 cut(s) 267, 473
MslI CAYNNNNRTG 2 cut(s) 441, 680
MspA1I CMGCKG 2 cut(s) 709, 812
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 3 cut(s) 151, 166, 750
MspR9I CCNGG 5 cut(s) 113, 281, 702, 732, 751
MunI CAATTG 1 cut(s) 33
MvaI CCWGG 4 cut(s) 113, 281, 702, 732
MvnI CGCG 1 cut(s) 800
MwoI GCNNNNNNNGC 8 cut(s) 92, 542, 563, 578, 587, 653, 782, 806
NaeI GCCGGC 1 cut(s) 167
NciI CCSGG 1 cut(s) 751
NcoI CCATGG 1 cut(s) 675
NgoMIV GCCGGC 1 cut(s) 165
NlaIII CATG 4 cut(s) 679, 761, 793, 830
OliI CACNNNNGTG 1 cut(s) 441
PagI TCATGA 1 cut(s) 757
PdiI GCCGGC 1 cut(s) 167
PfeI GAWTC 3 cut(s) 154, 738, 754
PflMI CCANNNNNTGG 1 cut(s) 832
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 9 cut(s) 85, 424, 451, 538, 613, 670, 673, 811, 814
PleI GAGTC 1 cut(s) 648
PpsI GAGTC 1 cut(s) 648
Psp6I CCWGG 4 cut(s) 111, 279, 700, 730
PspGI CCWGG 4 cut(s) 111, 279, 700, 730
PstI CTGCAG 1 cut(s) 616
PvuII CAGCTG 2 cut(s) 709, 812
RsaI GTAC 2 cut(s) 123, 562
RsaNI GTAC 2 cut(s) 122, 561
RseI CAYNNNNRTG 2 cut(s) 441, 680
SaqAI TTAA 2 cut(s) 267, 473
SatI GCNGC 9 cut(s) 84, 423, 450, 537, 612, 669, 672, 810, 813
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 5 cut(s) 113, 281, 702, 732, 751
SfaNI GCATC 3 cut(s) 523, 581, 734
SfcI CTRYAG 4 cut(s) 353, 426, 576, 612
SmiMI CAYNNNNRTG 2 cut(s) 441, 680
SmlI CTYRAG 3 cut(s) 266, 651, 764
SmoI CTYRAG 3 cut(s) 266, 651, 764
Sse9I AATT 7 cut(s) 33, 52, 77, 135, 189, 524, 630
SsiI CCGC 2 cut(s) 402, 800
StyD4I CCNGG 5 cut(s) 111, 279, 700, 730, 749
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 3 cut(s) 430, 457, 467
TaqI TCGA 1 cut(s) 133
TasI AATT 7 cut(s) 33, 52, 77, 135, 189, 524, 630
TfiI GAWTC 3 cut(s) 154, 738, 754
Tru1I TTAA 2 cut(s) 267, 473
Tru9I TTAA 2 cut(s) 267, 473
TscAI CASTG 1 cut(s) 255
TseI GCWGC 9 cut(s) 83, 422, 449, 536, 611, 668, 671, 809, 812
TspDTI ATGAA 4 cut(s) 52, 698, 746, 809
TspRI CASTG 1 cut(s) 255
Van91I CCANNNNNTGG 1 cut(s) 832
Vha464I CTTAAG 1 cut(s) 266
XapI RAATTY 3 cut(s) 135, 189, 630
XmiI GTMKAC 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.