pycom12g10380

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0024420
Physical Location & Seq
Reverse (-)
12354039 .. 12354713
675 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGTATTTCGTTTTCGGTATAGTTGCTGGTGCCTTCACCATGCATGCTAAGGATCGCGCGTCTGGCCGCAGCAAGTCTGATAATTGGGTGGGGGATATTATATTTGCGTTCAACAAAACTGCTACTTGCTTATATTTTACCAGCGTGACTTTATCAACTGTTGGTTACGGAGACATAGTTCCCCATGACGGAGATAAAGTTGCCATGTTGCTGACAATGCTCTTGATGTTATTGGGTTACTACCTTGTCAGTTTTGTGATTGCCATGGAAGTTGACAGATTGGGTAGTTGGTTGTTGATGCGCTTCCATGAGAGAAGAAGAGGATTCATAAGATTTTTCTGGGCGTGTCTTTGCGGGCCGATGCTCATAGCTTCTGGAGGTGTTATGATTAGGTATATGGAGGGGATTTCGTGGGCCGATTCCTTCTATCTATCAGTGGCGTCTGTCACAACTACAGGCTACGGGGATAAGCATTTCAACACATTGTTGGGCAAGTGTTTTGCTAGCATATGGCTTCTGGTCAGCACCATCCTGAGTAGTAAATCTGCACAGTTTATGTTTGACGTGATCATGCGGATTAAAAGAGTGAGAGCTTGTCGTGACTGTGGCACCATTTGCCACACATGTGTTGTCGCGGACTTAACGTCACAAATACACGCAGTACGCATTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

225

Amino Acids

25.05

Weight (kDa)

9.07

Isoelectric Point (pI)

25.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 5 - 90 3.5e-06 Ion transport protein
Ion_trans_2 PF07885 39 - 91 5.8e-11 Ion channel
Ion_trans_2 PF07885 122 - 190 3.7e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024420)

Species Orthologous Gene IDs
malus_domestica MD12G1105100.v1.1
pyrus_communis pycom12562g00030 pycom12g10380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 29, 608
AccII CGCG 3 cut(s) 57, 59, 635
AciI CCGC 4 cut(s) 67, 354, 574, 635
AclWI GGATC 1 cut(s) 60
AcoI YGGCCR 1 cut(s) 64
AcyI GRCGYC 1 cut(s) 440
AfaI GTAC 1 cut(s) 663
AfiI CCNNNNNNNGG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 623
AgsI TTSAA 2 cut(s) 112, 478
AhdI GACNNNNNGTC 1 cut(s) 643
AjiI CACGTC 1 cut(s) 565
AleI CACNNNNGTG 1 cut(s) 624
AloI GAACNNNNNNTCC 2 cut(s) 162, 194
AluBI AGCT 2 cut(s) 371, 593
AluI AGCT 2 cut(s) 371, 593
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 1 cut(s) 60
AoxI GGCC 3 cut(s) 64, 356, 414
ApeKI GCWGC 1 cut(s) 69
AspLEI GCGC 2 cut(s) 59, 303
AspS9I GGNCC 2 cut(s) 356, 414
AsuHPI GGTGA 1 cut(s) 28
AsuNHI GCTAGC 1 cut(s) 503
BanI GGYRCC 2 cut(s) 29, 608
BbvI GCAGC 1 cut(s) 81
BccI CCATC 1 cut(s) 536
BclI TGATCA 1 cut(s) 567
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 1 cut(s) 504
BfmI CTRYAG 1 cut(s) 453
BisI GCNGC 2 cut(s) 67, 70
BlsI GCNGC 2 cut(s) 68, 71
BmeRI GACNNNNNGTC 1 cut(s) 643
BmgBI CACGTC 1 cut(s) 565
BmgT120I GGNCC 2 cut(s) 356, 414
BmiI GGNNCC 2 cut(s) 31, 610
BmsI GCATC 2 cut(s) 288, 351
BmtI GCTAGC 1 cut(s) 507
BpmI CTGGAG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 48
BsaHI GRCGYC 1 cut(s) 440
BsaJI CCNNGG 1 cut(s) 264
BsaXI ACNNNNNCTCC 4 cut(s) 162, 183, 192, 213
Bsc4I CCNNNNNNNGG 1 cut(s) 188
BseDI CCNNGG 1 cut(s) 264
BseGI GGATG 1 cut(s) 528
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMII CTCAG 1 cut(s) 524
BseXI GCAGC 1 cut(s) 81
BsgI GTGCAG 1 cut(s) 531
Bsh1236I CGCG 3 cut(s) 57, 59, 635
BshFI GGCC 3 cut(s) 66, 358, 416
BshNI GGYRCC 2 cut(s) 29, 608
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 165
BsnI GGCC 3 cut(s) 66, 358, 416
Bsp143I GATC 2 cut(s) 52, 567
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 4 cut(s) 67, 354, 574, 635
BspANI GGCC 3 cut(s) 66, 358, 416
BspCNI CTCAG 1 cut(s) 525
BspFNI CGCG 3 cut(s) 57, 59, 635
BspLI GGNNCC 2 cut(s) 31, 610
BspOI GCTAGC 1 cut(s) 507
BspPI GGATC 1 cut(s) 60
BspT107I GGYRCC 2 cut(s) 29, 608
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 2 cut(s) 52, 567
BssNI GRCGYC 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 264
Bst4CI ACNGT 3 cut(s) 160, 552, 605
Bst6I CTCTTC 1 cut(s) 313
BstACI GRCGYC 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 615
BstC8I GCNNGC 3 cut(s) 45, 356, 505
BstDEI CTNAG 2 cut(s) 48, 533
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 1 cut(s) 528
BstFNI CGCG 3 cut(s) 57, 59, 635
BstHHI GCGC 2 cut(s) 59, 303
BstKTI GATC 2 cut(s) 55, 570
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 2 cut(s) 52, 567
BstMWI GCNNNNNNNGC 3 cut(s) 63, 217, 615
BstNSI RCATGY 2 cut(s) 47, 627
BstSFI CTRYAG 1 cut(s) 453
BstUI CGCG 3 cut(s) 57, 59, 635
BstV1I GCAGC 1 cut(s) 81
BsuRI GGCC 3 cut(s) 66, 358, 416
BtgI CCRYGG 1 cut(s) 264
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 1 cut(s) 528
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 3 cut(s) 45, 356, 505
CfoI GCGC 2 cut(s) 59, 303
Cfr13I GGNCC 2 cut(s) 356, 414
CseI GACGC 2 cut(s) 48, 429
Csp6I GTAC 1 cut(s) 662
CviAII CATG 8 cut(s) 40, 44, 185, 205, 265, 308, 571, 624
CviJI RGCY 7 cut(s) 66, 358, 371, 416, 459, 514, 593
CviKI_1 RGCY 7 cut(s) 66, 358, 371, 416, 459, 514, 593
CviQI GTAC 1 cut(s) 662
DdeI CTNAG 2 cut(s) 48, 533
DpnI GATC 2 cut(s) 54, 569
DpnII GATC 2 cut(s) 52, 567
DriI GACNNNNNGTC 1 cut(s) 643
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 313
Eam1105I GACNNNNNGTC 1 cut(s) 643
EarI CTCTTC 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 264
EcoT22I ATGCAT 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 8 cut(s) 43, 47, 188, 208, 268, 311, 574, 627
FatI CATG 8 cut(s) 39, 43, 184, 204, 264, 307, 570, 623
FauI CCCGC 1 cut(s) 347
FauNDI CATATG 1 cut(s) 509
FbaI TGATCA 1 cut(s) 567
Fnu4HI GCNGC 2 cut(s) 67, 70
FokI GGATG 1 cut(s) 515
Fsp4HI GCNGC 2 cut(s) 67, 70
FspBI CTAG 1 cut(s) 504
GlaI GCGC 2 cut(s) 58, 302
GluI GCNGC 2 cut(s) 67, 70
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 3 cut(s) 66, 358, 416
HgaI GACGC 2 cut(s) 48, 429
HhaI GCGC 2 cut(s) 59, 303
Hin1I GRCGYC 1 cut(s) 440
Hin1II CATG 8 cut(s) 43, 47, 188, 208, 268, 311, 574, 627
Hin6I GCGC 2 cut(s) 57, 301
HinP1I GCGC 2 cut(s) 57, 301
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 2 cut(s) 324, 419
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 1 cut(s) 79
Hpy188III TCNNGA 4 cut(s) 223, 375, 532, 599
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 2 cut(s) 43, 433
HpyCH4III ACNGT 3 cut(s) 160, 552, 605
HpyCH4IV ACGT 2 cut(s) 564, 644
HpyCH4V TGCA 2 cut(s) 43, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 217, 615
HpyF3I CTNAG 2 cut(s) 48, 533
HpySE526I ACGT 2 cut(s) 564, 644
Hsp92I GRCGYC 1 cut(s) 440
Hsp92II CATG 8 cut(s) 43, 47, 188, 208, 268, 311, 574, 627
HspAI GCGC 2 cut(s) 57, 301
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 2 cut(s) 52, 567
LpnPI CCDG 8 cut(s) 12, 48, 154, 325, 360, 441, 503, 545
Lsp1109I GCAGC 1 cut(s) 81
LweI GCATC 2 cut(s) 288, 351
MaeI CTAG 1 cut(s) 504
MaeII ACGT 2 cut(s) 564, 644
MaeIII GTNAC 6 cut(s) 145, 164, 236, 445, 599, 645
MalI GATC 2 cut(s) 54, 569
MboI GATC 2 cut(s) 52, 567
MboII GAAGA 2 cut(s) 327, 330
MluCI AATT 1 cut(s) 82
MnlI CCTC 3 cut(s) 314, 371, 394
Mph1103I ATGCAT 1 cut(s) 45
MseI TTAA 2 cut(s) 579, 641
MslI CAYNNNNRTG 1 cut(s) 624
MvnI CGCG 3 cut(s) 57, 59, 635
MwoI GCNNNNNNNGC 3 cut(s) 63, 217, 615
NcoI CCATGG 1 cut(s) 264
NdeI CATATG 1 cut(s) 509
NdeII GATC 2 cut(s) 52, 567
NheI GCTAGC 1 cut(s) 503
NlaIII CATG 8 cut(s) 43, 47, 188, 208, 268, 311, 574, 627
NlaIV GGNNCC 2 cut(s) 31, 610
NmuCI GTSAC 4 cut(s) 145, 445, 599, 645
NsiI ATGCAT 1 cut(s) 45
NspI RCATGY 2 cut(s) 47, 627
OliI CACNNNNGTG 1 cut(s) 624
PaeI GCATGC 1 cut(s) 47
PciI ACATGT 1 cut(s) 623
PfeI GAWTC 2 cut(s) 324, 419
PkrI GCNGC 2 cut(s) 68, 71
PscI ACATGT 1 cut(s) 623
PspN4I GGNNCC 2 cut(s) 31, 610
PspPI GGNCC 2 cut(s) 356, 414
RsaI GTAC 1 cut(s) 663
RsaNI GTAC 1 cut(s) 662
RseI CAYNNNNRTG 1 cut(s) 624
SaqAI TTAA 2 cut(s) 579, 641
SatI GCNGC 2 cut(s) 67, 70
Sau3AI GATC 2 cut(s) 52, 567
Sau96I GGNCC 2 cut(s) 356, 414
SetI ASST 7 cut(s) 246, 373, 382, 395, 567, 595, 647
SfaNI GCATC 2 cut(s) 288, 351
SfcI CTRYAG 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 624
SphI GCATGC 1 cut(s) 47
Sse9I AATT 1 cut(s) 82
SsiI CCGC 4 cut(s) 67, 354, 574, 635
SspMI CTAG 1 cut(s) 504
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 3 cut(s) 160, 552, 605
TaiI ACGT 2 cut(s) 567, 647
TasI AATT 1 cut(s) 82
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 2 cut(s) 324, 419
Tru1I TTAA 2 cut(s) 579, 641
Tru9I TTAA 2 cut(s) 579, 641
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 4 cut(s) 145, 445, 599, 645
TseI GCWGC 1 cut(s) 69
Tsp45I GTSAC 4 cut(s) 145, 445, 599, 645
TspDTI ATGAA 1 cut(s) 316
TspGWI ACGGA 2 cut(s) 183, 204
TspRI CASTG 1 cut(s) 441
XceI RCATGY 2 cut(s) 47, 627
XspI CTAG 1 cut(s) 504
Zsp2I ATGCAT 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.