pycom12g11410

Ubiquitin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0028993
Physical Location & Seq
Forward (+)
13667964 .. 13668928
965 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11410.2

Sequence Viewer

Length: 774 bp
ATGGTGCTCTTAACAAATATAAGAGAACATACCACACCAGAGATTGAATATGAAGTACATTTTCTTGACTTTTTTCTTCGTGTGAAGGAGATAATTGAGAATCGTTCGAATATCGAAGTTCATAAGCATAGTCTTTGGCTTTATGACATAGAGCTAGAAAATGATCAACTTGTTGGACATCACATTGAAGGAGAACATGCTACTCTGAGGCTCATTATCACACCTCTGCTACCAGAATGGCCAAAATGTAGTATTTTGATCAAGTTTAGCAACCAAACTTCTGGCAACGTACGAATAAGAGATACAAGTTTTGTGGCCAATCTGAAAAGCAAAACCGGGAGGCGTTGGGGGTTTCTGAATACTGATAACATTGCTATTTTCCATGGTTCTACAAAAATGATGAATAACCGTCCTCTTTCTGCATATGATATAACCGACGGTTCTGAAGTTGCAGTTAAGATTGAGATTGACACTCCTATTTTAAGGAGACAAATTAGGAATCCTTTGGGATCAAGAAGATCTTTCCTAATAGACTTTATATTACTTGGAAAGCAAGTTTCTCTCCTCCTTATTACTATAAATAAAGGCACAAGGACTGGGGAATTAACACACAATTCCTCAAGCCTATTCTCCATTCTCTTTCTCTCCATGCTGCACCACTCAGTTAAATATAGGCCACAACACGTTATCAGCACGCTCATCCACCGCGCTAAGAAATTTGACGTGGTTATTCTTCAACTAGGGGAGTATTATGTTTCAATTATTTTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

258

Amino Acids

29.72

Weight (kDa)

9.16

Isoelectric Point (pI)

55.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028993)

Species Orthologous Gene IDs
prunus_persica Prupe.6G233900_v2.0.a1
pyrus_communis pycom12g11410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 708
AciI CCGC 1 cut(s) 706
AclWI GGATC 1 cut(s) 517
AcoI YGGCCR 2 cut(s) 239, 315
AcsI RAATTY 1 cut(s) 716
AcuI CTGAAG 1 cut(s) 465
AfaI GTAC 2 cut(s) 57, 291
AflIII ACRYGT 1 cut(s) 682
AgsI TTSAA 4 cut(s) 47, 188, 737, 759
AjiI CACGTC 1 cut(s) 724
AluBI AGCT 1 cut(s) 154
AluI AGCT 1 cut(s) 154
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 481
AlwI GGATC 1 cut(s) 517
AoxI GGCC 3 cut(s) 239, 315, 674
ApeKI GCWGC 1 cut(s) 652
ApoI RAATTY 1 cut(s) 716
AspLEI GCGC 1 cut(s) 710
AsuC2I CCSGG 1 cut(s) 337
AsuII TTCGAA 1 cut(s) 107
BalI TGGCCA 2 cut(s) 241, 317
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 1 cut(s) 639
BclI TGATCA 2 cut(s) 163, 258
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 1 cut(s) 481
BfaI CTAG 2 cut(s) 155, 740
BglII AGATCT 1 cut(s) 518
BisI GCNGC 1 cut(s) 653
BlsI GCNGC 1 cut(s) 654
Bme1390I CCNGG 1 cut(s) 337
BmgBI CACGTC 1 cut(s) 724
BmrFI CCNGG 1 cut(s) 337
BmrI ACTGGG 1 cut(s) 606
BmuI ACTGGG 1 cut(s) 606
Bpu14I TTCGAA 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 337
BsaJI CCNNGG 1 cut(s) 382
Bse1I ACTGG 1 cut(s) 601
Bse3DI GCAATG 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 382
BseGI GGATG 1 cut(s) 699
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 2 cut(s) 197, 675
BseNI ACTGG 1 cut(s) 601
BseRI GAGGAG 1 cut(s) 554
BseXI GCAGC 1 cut(s) 639
BsgI GTGCAG 1 cut(s) 638
Bsh1236I CGCG 1 cut(s) 708
BshFI GGCC 3 cut(s) 241, 317, 676
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 336
BsiWI CGTACG 1 cut(s) 289
BsmAI GTCTC 1 cut(s) 481
BsnI GGCC 3 cut(s) 241, 317, 676
Bsp119I TTCGAA 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 4 cut(s) 163, 258, 509, 518
Bsp19I CCATGG 1 cut(s) 382
BspACI CCGC 1 cut(s) 706
BspANI GGCC 3 cut(s) 241, 317, 676
BspCNI CTCAG 2 cut(s) 198, 674
BspFNI CGCG 1 cut(s) 708
BspPI GGATC 1 cut(s) 517
BspT104I TTCGAA 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 1 cut(s) 601
BssECI CCNNGG 1 cut(s) 382
BssMI GATC 4 cut(s) 163, 258, 509, 518
BssT1I CCWWGG 1 cut(s) 382
Bst4CI ACNGT 2 cut(s) 410, 440
BstBI TTCGAA 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 695
BstDEI CTNAG 3 cut(s) 206, 661, 711
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 1 cut(s) 699
BstFNI CGCG 1 cut(s) 708
BstHHI GCGC 1 cut(s) 710
BstKTI GATC 4 cut(s) 166, 261, 512, 521
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 4 cut(s) 163, 258, 509, 518
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 1 cut(s) 335
BstUI CGCG 1 cut(s) 708
BstV1I GCAGC 1 cut(s) 639
BstX2I RGATCY 1 cut(s) 518
BstXI CCANNNNNNTGG 1 cut(s) 281
BstYI RGATCY 1 cut(s) 518
BsuRI GGCC 3 cut(s) 241, 317, 676
BtgI CCRYGG 1 cut(s) 382
BtrI CACGTC 1 cut(s) 724
BtsCI GGATG 1 cut(s) 699
Cac8I GCNNGC 1 cut(s) 695
CfoI GCGC 1 cut(s) 710
Csp6I GTAC 2 cut(s) 56, 290
CspCI CAANNNNNGTGG 2 cut(s) 294, 329
CviAII CATG 3 cut(s) 197, 383, 649
CviJI RGCY 7 cut(s) 139, 154, 211, 241, 317, 624, 676
CviKI_1 RGCY 7 cut(s) 139, 154, 211, 241, 317, 624, 676
CviQI GTAC 2 cut(s) 56, 290
DdeI CTNAG 3 cut(s) 206, 661, 711
DpnI GATC 4 cut(s) 165, 260, 511, 520
DpnII GATC 4 cut(s) 163, 258, 509, 518
EaeI YGGCCR 2 cut(s) 239, 315
Eco130I CCWWGG 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 465
EcoT14I CCWWGG 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 3 cut(s) 200, 386, 652
FalI AAGNNNNNCTT 2 cut(s) 505, 537
FatI CATG 3 cut(s) 196, 382, 648
FauNDI CATATG 1 cut(s) 424
FbaI TGATCA 2 cut(s) 163, 258
Fnu4HI GCNGC 1 cut(s) 653
FokI GGATG 1 cut(s) 686
Fsp4HI GCNGC 1 cut(s) 653
FspBI CTAG 2 cut(s) 155, 740
GlaI GCGC 1 cut(s) 709
GluI GCNGC 1 cut(s) 653
HaeIII GGCC 3 cut(s) 241, 317, 676
HapII CCGG 1 cut(s) 336
HhaI GCGC 1 cut(s) 710
Hin1II CATG 3 cut(s) 200, 386, 652
Hin6I GCGC 1 cut(s) 708
HinP1I GCGC 1 cut(s) 708
HinfI GANTC 2 cut(s) 100, 499
HpaII CCGG 1 cut(s) 336
Hpy188I TCNGA 4 cut(s) 207, 324, 357, 445
Hpy188III TCNNGA 2 cut(s) 65, 513
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 2 cut(s) 79, 182
HpyCH4III ACNGT 2 cut(s) 410, 440
HpyCH4IV ACGT 3 cut(s) 288, 684, 723
HpyCH4V TGCA 3 cut(s) 422, 452, 655
HpyF3I CTNAG 3 cut(s) 206, 661, 711
HpySE526I ACGT 3 cut(s) 288, 684, 723
Hsp92II CATG 3 cut(s) 200, 386, 652
HspAI GCGC 1 cut(s) 708
Ksp22I TGATCA 2 cut(s) 163, 258
Kzo9I GATC 4 cut(s) 163, 258, 509, 518
LpnPI CCDG 5 cut(s) 51, 246, 267, 349, 582
Lsp1109I GCAGC 1 cut(s) 639
MaeI CTAG 2 cut(s) 155, 740
MaeII ACGT 3 cut(s) 288, 684, 723
MalI GATC 4 cut(s) 165, 260, 511, 520
MboI GATC 4 cut(s) 163, 258, 509, 518
MboII GAAGA 3 cut(s) 68, 528, 725
MflI RGATCY 1 cut(s) 518
MhlI GDGCHC 1 cut(s) 9
MlsI TGGCCA 2 cut(s) 241, 317
MluCI AATT 7 cut(s) 93, 492, 602, 613, 716, 759, 769
MluNI TGGCCA 2 cut(s) 241, 317
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 6 cut(s) 201, 234, 333, 423, 575, 628
Mox20I TGGCCA 2 cut(s) 241, 317
MscI TGGCCA 2 cut(s) 241, 317
MseI TTAA 6 cut(s) 11, 456, 482, 605, 666, 768
Msp20I TGGCCA 2 cut(s) 241, 317
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 1 cut(s) 337
MvnI CGCG 1 cut(s) 708
NciI CCSGG 1 cut(s) 337
NcoI CCATGG 1 cut(s) 382
NdeI CATATG 1 cut(s) 424
NdeII GATC 4 cut(s) 163, 258, 509, 518
NlaIII CATG 3 cut(s) 200, 386, 652
NspI RCATGY 1 cut(s) 200
NspV TTCGAA 1 cut(s) 107
PfeI GAWTC 2 cut(s) 100, 499
Pfl23II CGTACG 1 cut(s) 289
PkrI GCNGC 1 cut(s) 654
PspLI CGTACG 1 cut(s) 289
PsuI RGATCY 1 cut(s) 518
RsaI GTAC 2 cut(s) 57, 291
RsaNI GTAC 2 cut(s) 56, 290
SaqAI TTAA 6 cut(s) 11, 456, 482, 605, 666, 768
SatI GCNGC 1 cut(s) 653
Sau3AI GATC 4 cut(s) 163, 258, 509, 518
ScrFI CCNGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 9
SetI ASST 5 cut(s) 156, 226, 291, 687, 726
SfuI TTCGAA 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
Sse9I AATT 7 cut(s) 93, 492, 602, 613, 716, 759, 769
SsiI CCGC 1 cut(s) 706
SspMI CTAG 2 cut(s) 155, 740
StyD4I CCNGG 1 cut(s) 335
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 2 cut(s) 410, 440
TaiI ACGT 3 cut(s) 291, 687, 726
TaqI TCGA 2 cut(s) 107, 114
TasI AATT 7 cut(s) 93, 492, 602, 613, 716, 759, 769
TatI WGTACW 1 cut(s) 55
TfiI GAWTC 2 cut(s) 100, 499
Tru1I TTAA 6 cut(s) 11, 456, 482, 605, 666, 768
Tru9I TTAA 6 cut(s) 11, 456, 482, 605, 666, 768
TseI GCWGC 1 cut(s) 652
TspDTI ATGAA 3 cut(s) 66, 110, 416
XapI RAATTY 1 cut(s) 716
XceI RCATGY 1 cut(s) 200
XspI CTAG 2 cut(s) 155, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.