pycom12g14000

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0029431
Physical Location & Seq
Reverse (-)
16439905 .. 16440495
591 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g14000.2

Sequence Viewer

Length: 501 bp
ATGGATGATCCTTCCTCCACCAACTCTGTCAATGGCTTCTTTTCCTTTCTGGCTCGTGGGGTGGACGATCTCGAAAGGGTCTATCTTTCCAGCAACTTCATGTCAATCCAATTCCTTCAAGGGGTTCTTACCCTTCTTCGATCGATCCACTCCCAGCTGACCCTTATTGTCCAGAGGCTCCATCTGCCTGTTGGGGACAAGTGGCTTGACGAGTACATGGACGAAAGCTCCAAGCTTTGGGAAGCCTGCCATGTCCTCAAATCCGCCGTCTCTGCCATGGAGAATTTCTACACCTCCGGCCACACCATCACCTCCACCCTCCTCGACTCTCACCACCATCTCACTCCTCAGCTCTCCCGCCAGGTGGTGCGCGCGATTTCTCGGTGGCGGCGGGAAGCGTTTGGATTGGAGGAAGAGAACAGAGCGCTGATGGAAACGAGAATCCAACCACTCTCACTGAAATTCGACGAAAGGATCGATCGCCGTCGAATCGAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

167

Amino Acids

19.24

Weight (kDa)

6.15

Isoelectric Point (pI)

56.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029431)

Species Orthologous Gene IDs
pyrus_communis pycom12g14000
rosa_samantha Rh7DG474500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 237
AccII CGCG 2 cut(s) 372, 374
AciI CCGC 4 cut(s) 264, 358, 388, 391
AclWI GGATC 3 cut(s) 2, 139, 482
AcoI YGGCCR 1 cut(s) 298
AcsI RAATTY 2 cut(s) 283, 461
AfaI GTAC 1 cut(s) 215
AfeI AGCGCT 1 cut(s) 426
AfiI CCNNNNNNNGG 3 cut(s) 121, 237, 364
AgsI TTSAA 1 cut(s) 119
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 5 cut(s) 157, 228, 235, 352, 497
AluI AGCT 5 cut(s) 157, 228, 235, 352, 497
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 3 cut(s) 2, 139, 482
Aor51HI AGCGCT 1 cut(s) 426
AoxI GGCC 1 cut(s) 298
ApoI RAATTY 2 cut(s) 283, 461
ArsI GACNNNNNNTTYG 2 cut(s) 252, 284
AspLEI GCGC 3 cut(s) 372, 374, 427
AsuHPI GGTGA 2 cut(s) 301, 323
BauI CACGAG 1 cut(s) 54
BbvCI CCTCAGC 1 cut(s) 348
BccI CCATC 4 cut(s) 189, 314, 345, 424
BceAI ACGGC 2 cut(s) 251, 468
BciT130I CCWGG 1 cut(s) 362
BcoDI GTCTC 1 cut(s) 274
BfoI RGCGCY 1 cut(s) 428
BisI GCNGC 1 cut(s) 389
BlsI GCNGC 1 cut(s) 390
Bme1390I CCNGG 1 cut(s) 362
BmiI GGNNCC 1 cut(s) 179
BmrFI CCNGG 1 cut(s) 362
Bpu10I CCTNAGC 1 cut(s) 348
Bsa29I ATCGAT 2 cut(s) 143, 477
BsaJI CCNNGG 1 cut(s) 276
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 237, 364
BseBI CCWGG 1 cut(s) 362
BseCI ATCGAT 2 cut(s) 143, 477
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 3 cut(s) 121, 237, 364
BseMII CTCAG 1 cut(s) 362
BsePI GCGCGC 1 cut(s) 370
BseRI GAGGAG 2 cut(s) 311, 336
BseYI CCCAGC 1 cut(s) 153
Bsh1236I CGCG 2 cut(s) 372, 374
Bsh1285I CGRYCG 2 cut(s) 143, 481
BshFI GGCC 1 cut(s) 300
BshVI ATCGAT 2 cut(s) 143, 477
BsiEI CGRYCG 2 cut(s) 143, 481
BsiSI CCGG 1 cut(s) 297
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 3 cut(s) 121, 237, 364
BsmAI GTCTC 1 cut(s) 274
BsmBI CGTCTC 1 cut(s) 274
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 1 cut(s) 300
Bsp143I GATC 6 cut(s) 7, 67, 140, 144, 474, 478
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 4 cut(s) 264, 358, 388, 391
BspANI GGCC 1 cut(s) 300
BspCNI CTCAG 1 cut(s) 361
BspDI ATCGAT 2 cut(s) 143, 477
BspFNI CGCG 2 cut(s) 372, 374
BspLI GGNNCC 1 cut(s) 179
BspPI GGATC 3 cut(s) 2, 139, 482
BssECI CCNNGG 1 cut(s) 276
BssHII GCGCGC 1 cut(s) 370
BssMI GATC 6 cut(s) 7, 67, 140, 144, 474, 478
BssSI CACGAG 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 362
Bst6I CTCTTC 1 cut(s) 408
BstC8I GCNNGC 2 cut(s) 247, 372
BstDEI CTNAG 1 cut(s) 348
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 2 cut(s) 372, 374
BstH2I RGCGCY 1 cut(s) 428
BstHHI GCGC 3 cut(s) 372, 374, 427
BstKTI GATC 6 cut(s) 10, 70, 143, 147, 477, 481
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 6 cut(s) 7, 67, 140, 144, 474, 478
BstMCI CGRYCG 2 cut(s) 143, 481
BstMWI GCNNNNNNNGC 2 cut(s) 184, 272
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 360
BstUI CGCG 2 cut(s) 372, 374
Bsu15I ATCGAT 2 cut(s) 143, 477
BsuRI GGCC 1 cut(s) 300
BsuTUI ATCGAT 2 cut(s) 143, 477
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 455
Cac8I GCNNGC 2 cut(s) 247, 372
CfoI GCGC 3 cut(s) 372, 374, 427
ClaI ATCGAT 2 cut(s) 143, 477
Csp6I GTAC 1 cut(s) 214
CviAII CATG 4 cut(s) 100, 217, 251, 277
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 348
DpnI GATC 6 cut(s) 9, 69, 142, 146, 476, 480
DpnII GATC 6 cut(s) 7, 67, 140, 144, 474, 478
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EciI GGCGGA 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 276
Eco47III AGCGCT 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 1 cut(s) 274
FaeI CATG 4 cut(s) 103, 220, 254, 280
FaiI YATR 4 cut(s) 101, 218, 252, 278
FalI AAGNNNNNCTT 2 cut(s) 111, 143
FaqI GGGAC 1 cut(s) 209
FatI CATG 4 cut(s) 99, 216, 250, 276
FauI CCCGC 2 cut(s) 365, 384
Fnu4HI GCNGC 1 cut(s) 389
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 389
GlaI GCGC 3 cut(s) 371, 373, 426
GluI GCNGC 1 cut(s) 389
GsaI CCCAGC 1 cut(s) 157
HaeII RGCGCY 1 cut(s) 428
HaeIII GGCC 1 cut(s) 300
HapII CCGG 1 cut(s) 297
HhaI GCGC 3 cut(s) 372, 374, 427
Hin1II CATG 4 cut(s) 103, 220, 254, 280
Hin6I GCGC 3 cut(s) 370, 372, 425
HinP1I GCGC 3 cut(s) 370, 372, 425
HindIII AAGCTT 2 cut(s) 233, 495
HinfI GANTC 3 cut(s) 326, 441, 489
HpaII CCGG 1 cut(s) 297
HphI GGTGA 2 cut(s) 301, 323
Hpy166II GTNNAC 1 cut(s) 64
Hpy188III TCNNGA 2 cut(s) 71, 172
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 2 cut(s) 470, 489
HpyAV CCTTC 3 cut(s) 21, 125, 143
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 272
HpyF3I CTNAG 1 cut(s) 348
Hsp92II CATG 4 cut(s) 103, 220, 254, 280
HspAI GCGC 3 cut(s) 370, 372, 425
Kzo9I GATC 6 cut(s) 7, 67, 140, 144, 474, 478
LmnI GCTCC 2 cut(s) 183, 233
LpnPI CCDG 9 cut(s) 35, 103, 167, 185, 201, 259, 310, 347, 374
MalI GATC 6 cut(s) 9, 69, 142, 146, 476, 480
MboI GATC 6 cut(s) 7, 67, 140, 144, 474, 478
MboII GAAGA 2 cut(s) 128, 425
MluCI AATT 3 cut(s) 110, 283, 461
MlyI GAGTC 1 cut(s) 320
MmeI TCCRAC 1 cut(s) 469
MnlI CCTC 9 cut(s) 25, 168, 266, 304, 322, 329, 332, 357, 403
MseI TTAA 1 cut(s) 499
MspA1I CMGCKG 1 cut(s) 157
MspI CCGG 1 cut(s) 297
MspR9I CCNGG 1 cut(s) 362
MvaI CCWGG 1 cut(s) 362
MvnI CGCG 2 cut(s) 372, 374
MwoI GCNNNNNNNGC 2 cut(s) 184, 272
NcoI CCATGG 1 cut(s) 276
NdeII GATC 6 cut(s) 7, 67, 140, 144, 474, 478
NlaIII CATG 4 cut(s) 103, 220, 254, 280
NlaIV GGNNCC 1 cut(s) 179
PauI GCGCGC 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PfeI GAWTC 2 cut(s) 441, 489
PflMI CCANNNNNTGG 1 cut(s) 237
PkrI GCNGC 1 cut(s) 390
Ple19I CGATCG 2 cut(s) 143, 481
PleI GAGTC 1 cut(s) 320
PpsI GAGTC 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 360
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 1 cut(s) 179
PteI GCGCGC 1 cut(s) 370
PvuI CGATCG 2 cut(s) 143, 481
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SaqAI TTAA 1 cut(s) 499
SatI GCNGC 1 cut(s) 389
Sau3AI GATC 6 cut(s) 7, 67, 140, 144, 474, 478
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 1 cut(s) 362
SetI ASST 8 cut(s) 159, 230, 237, 296, 314, 354, 366, 499
Sse9I AATT 3 cut(s) 110, 283, 461
SsiI CCGC 4 cut(s) 264, 358, 388, 391
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 1 cut(s) 276
TaqI TCGA 8 cut(s) 72, 139, 143, 324, 465, 477, 487, 492
TasI AATT 3 cut(s) 110, 283, 461
TatI WGTACW 1 cut(s) 213
TauI GCSGC 1 cut(s) 391
TfiI GAWTC 2 cut(s) 441, 489
Tru1I TTAA 1 cut(s) 499
Tru9I TTAA 1 cut(s) 499
TscAI CASTG 1 cut(s) 462
TspDTI ATGAA 1 cut(s) 88
TspRI CASTG 1 cut(s) 462
Van91I CCANNNNNTGG 1 cut(s) 237
XapI RAATTY 2 cut(s) 283, 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.