pycom12g17140

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0024414
Physical Location & Seq
Reverse (-)
19439466 .. 19440899
1434 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1434 bp
ATGGCTACAAGGCTGATGACAACAATGTTCTTTGTTTTTCGACATCACAAAAGGGACGGCTTACTTAGTGACAAAAATCGCAGAAGAGATGAGATCTCAAGTACCGCACCTTCTTCAAATAGCCAAGGTTTTCGAAAACGTTTCCATGGGATTCTTTCTCAGAAGATCGATTGGGAGTCCATGAGGAAAATATGCAAAAAGTGGATCAAGAACCCTCTGAATATGGCACTCCTTTTGTGGATAAGTTGTGTTGCTGTCTCCGGAGCAATCCTTTTTCTTGTGATGACAGGAATGTTAAACCATGTCCTGCCGGTGCAGTCTCAGAGAAATGCGTGGTTTGAGATTAACAATCAAATCATCAACGCTCTCTTCACTCTCATGAGTCTTTACCATCACCCAAAAAGGATCCACCACCTTGTACTTCTCTTAAGATGGAAGCCGAAAGATGTCACATTTCTCAGAAAAATATACTGTAAGAACGGAACTTACAAGCCCCATGAACGAATGCACATTCTGGTTGTTGTTGCATTCCTCCATCTGAATTGCTTTGCTCAGTATGCCTTGTGTACGCTTAACTTGGGATACAAAAGATCTGAGCGACCGGCTATTGGTGTCGGTATCTGCCTTTCAGTTGCAATTGCTGCTCCTGCAATCGCAGGTGTGTACTGCATTGTCAGCCCCCTTGGAAAGGAGGACGAAGTCGATAGCGAATCGCAGGATCATATTCTCACAAATCAAACGAGGAAATTTTCATTTGCATCTAGGAATAAGCAAGGGATTGCTGAATTTGCACCTCAATGGAGAGGGGGTTTATTTGATTTTCGGGATGATATTGCCATATCTTATCTCTCTTTTTTCTGCACCTTTTGTGTTTTTGGATGGAACGCCGAAAGGCTTGGATTTGGCAACATGTATGTTCACATTGCAACATTTCTCCTCTTCTGCACAGCCCCATTTTGGATCCTCAGTTTAGCTGCCTTCCATATTGACAACGACGTTGCAAGAGAAGCCATGACTTTAACCGGCATTGTCCTTTGTATGTTTGGCTTGCTCTACGGTGGCTTCTGGAGAATCCAAATGAGGAAGAGGTTCAACTTGCCGGCGAATAACTTATGCTGTGGAAAGCCTGCAGTAACAGACTCTGCACAATGGCTTTTCTGCTGCTGGTGCTCACTAGCTCAGGAAGTTCGAACTGCGAATTTCTACAGCATAATGGAAGATAACTTGTGCAGGAAGCAAATGTCTGAGCACGAAAATCCCTCACTAGTCCCCTTGCGGCGTGAAAACGGAAAAACTCAATATAGATCTGGCTCAAATTATCTGCCTTCAAGCAGAGTTGAAAAAGATTACCCTGATGTAGACAGACAGCTCCCCATGGAACCCCCTGTTCCATCCAGAATACAGAGAGAAGACAAAATCGAACCGAAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

478

Amino Acids

54.93

Weight (kDa)

9.36

Isoelectric Point (pI)

55.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2985 PF11204 67 - 145 5.7e-28 Protein of unknown function (DUF2985)
PLAC8 PF04749 267 - 397 3.3e-19 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024414)

Species Orthologous Gene IDs
malus_domestica MD04G1168700.v1.1 MD12G1182300.v1.1
pyrus_communis pycom12g17140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 647
Acc36I ACCTGC 1 cut(s) 647
AccI GTMKAC 1 cut(s) 1359
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 2 cut(s) 105, 1276
AclI AACGTT 1 cut(s) 139
AclWI GGATC 6 cut(s) 212, 400, 413, 726, 955, 968
AcsI RAATTY 3 cut(s) 746, 785, 1198
AfaI GTAC 4 cut(s) 103, 420, 568, 665
AfiI CCNNNNNNNGG 3 cut(s) 608, 688, 957
AflII CTTAAG 1 cut(s) 427
AflIII ACRYGT 1 cut(s) 909
AgsI TTSAA 4 cut(s) 117, 1093, 1329, 1340
AhlI ACTAGT 1 cut(s) 1264
AjiI CACGTC 1 cut(s) 1431
AluBI AGCT 3 cut(s) 974, 1178, 1369
AluI AGCT 3 cut(s) 974, 1178, 1369
Alw21I GWGCWC 2 cut(s) 1172, 1251
Alw26I GTCTC 2 cut(s) 262, 324
AlwI GGATC 6 cut(s) 212, 400, 413, 726, 955, 968
AlwNI CAGNNNCTG 1 cut(s) 1142
Aor13HI TCCGGA 1 cut(s) 260
ApeKI GCWGC 3 cut(s) 641, 974, 1161
ApoI RAATTY 3 cut(s) 746, 785, 1198
Asp700I GAANNNNTTC 1 cut(s) 1088
AsuHPI GGTGA 1 cut(s) 386
AsuII TTCGAA 2 cut(s) 133, 1189
BamHI GGATCC 2 cut(s) 405, 960
BarI GAAGNNNNNNTAC 2 cut(s) 94, 126
BbsI GAAGAC 1 cut(s) 1416
Bbv12I GWGCWC 2 cut(s) 1172, 1251
BbvI GCAGC 3 cut(s) 628, 961, 1148
BccI CCATC 5 cut(s) 399, 426, 543, 873, 1399
BceAI ACGGC 1 cut(s) 73
BciVI GTATCC 1 cut(s) 575
BcoDI GTCTC 2 cut(s) 262, 324
BcuI ACTAGT 1 cut(s) 1264
BfaI CTAG 3 cut(s) 762, 1175, 1265
BfmI CTRYAG 2 cut(s) 1128, 1204
BfrI CTTAAG 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 647
BfuI GTATCC 1 cut(s) 575
BglII AGATCT 3 cut(s) 93, 590, 1304
BisI GCNGC 4 cut(s) 642, 975, 1162, 1277
BlsI GCNGC 4 cut(s) 643, 976, 1163, 1278
BmgBI CACGTC 1 cut(s) 1431
BmiI GGNNCC 3 cut(s) 407, 962, 1380
BmsI GCATC 1 cut(s) 767
BpiI GAAGAC 1 cut(s) 1416
BpmI CTGGAG 1 cut(s) 1087
Bpu10I CCTNAGC 1 cut(s) 1179
Bpu14I TTCGAA 2 cut(s) 133, 1189
BpuEI CTTGAG 1 cut(s) 82
Bsa29I ATCGAT 1 cut(s) 168
BsaJI CCNNGG 4 cut(s) 124, 145, 682, 1374
BsaWI WCCGGW 1 cut(s) 260
BsaXI ACNNNNNCTCC 2 cut(s) 793, 823
Bsc4I CCNNNNNNNGG 3 cut(s) 608, 688, 957
Bse118I RCCGGY 4 cut(s) 310, 601, 1022, 1099
Bse3DI GCAATG 1 cut(s) 921
BseAI TCCGGA 1 cut(s) 260
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 4 cut(s) 124, 145, 682, 1374
BseGI GGATG 3 cut(s) 832, 884, 1391
BseLI CCNNNNNNNGG 3 cut(s) 608, 688, 957
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 8 cut(s) 173, 335, 472, 566, 585, 979, 1193, 1236
BseRI GAGGAG 1 cut(s) 926
BseXI GCAGC 3 cut(s) 628, 961, 1148
BsgI GTGCAG 5 cut(s) 335, 844, 928, 1128, 1249
Bsh1285I CGRYCG 1 cut(s) 602
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 602
BsiHKAI GWGCWC 2 cut(s) 1172, 1251
BsiSI CCGG 5 cut(s) 261, 311, 602, 1023, 1100
BslFI GGGAC 2 cut(s) 68, 1253
BslI CCNNNNNNNGG 3 cut(s) 608, 688, 957
BsmAI GTCTC 2 cut(s) 262, 324
BsmFI GGGAC 2 cut(s) 68, 1253
BsmI GAATGC 2 cut(s) 510, 527
Bsp119I TTCGAA 2 cut(s) 133, 1189
Bsp1286I GDGCHC 2 cut(s) 1172, 1251
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 8 cut(s) 93, 165, 204, 405, 590, 718, 960, 1304
Bsp19I CCATGG 2 cut(s) 145, 1374
BspACI CCGC 2 cut(s) 105, 1276
BspCNI CTCAG 8 cut(s) 172, 334, 471, 565, 586, 978, 1192, 1237
BspDI ATCGAT 1 cut(s) 168
BspEI TCCGGA 1 cut(s) 260
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 3 cut(s) 407, 962, 1380
BspMAI CTGCAG 1 cut(s) 1132
BspMI ACCTGC 1 cut(s) 647
BspPI GGATC 6 cut(s) 212, 400, 413, 726, 955, 968
BspT104I TTCGAA 2 cut(s) 133, 1189
BspTI CTTAAG 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 921
BsrFI RCCGGY 4 cut(s) 310, 601, 1022, 1099
BssAI RCCGGY 4 cut(s) 310, 601, 1022, 1099
BssECI CCNNGG 4 cut(s) 124, 145, 682, 1374
BssMI GATC 8 cut(s) 93, 165, 204, 405, 590, 718, 960, 1304
BssT1I CCWWGG 4 cut(s) 124, 145, 682, 1374
Bst4CI ACNGT 2 cut(s) 473, 1058
Bst6I CTCTTC 4 cut(s) 79, 374, 944, 1079
BstAFI CTTAAG 1 cut(s) 427
BstAPI GCANNNNNTGC 1 cut(s) 641
BstBI TTCGAA 2 cut(s) 133, 1189
BstC8I GCNNGC 3 cut(s) 1049, 1101, 1128
BstDEI CTNAG 9 cut(s) 65, 159, 321, 458, 552, 594, 965, 1179, 1245
BstDSI CCRYGG 2 cut(s) 145, 1374
BstENI CCTNNNNNAGG 1 cut(s) 686
BstF5I GGATG 3 cut(s) 832, 884, 1391
BstKTI GATC 8 cut(s) 96, 168, 207, 408, 593, 721, 963, 1307
BstMAI GTCTC 2 cut(s) 262, 324
BstMBI GATC 8 cut(s) 93, 165, 204, 405, 590, 718, 960, 1304
BstMCI CGRYCG 1 cut(s) 602
BstMWI GCNNNNNNNGC 7 cut(s) 557, 641, 647, 675, 788, 1007, 1167
BstNSI RCATGY 1 cut(s) 913
BstSFI CTRYAG 2 cut(s) 1128, 1204
BstV1I GCAGC 3 cut(s) 628, 961, 1148
BstV2I GAAGAC 1 cut(s) 1416
BstX2I RGATCY 5 cut(s) 93, 405, 590, 960, 1304
BstYI RGATCY 5 cut(s) 93, 405, 590, 960, 1304
Bsu15I ATCGAT 1 cut(s) 168
BsuI GTATCC 1 cut(s) 575
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 2 cut(s) 145, 1374
BtrI CACGTC 1 cut(s) 1431
BtsCI GGATG 3 cut(s) 832, 884, 1391
BveI ACCTGC 1 cut(s) 647
Cac8I GCNNGC 3 cut(s) 1049, 1101, 1128
CaiI CAGNNNCTG 1 cut(s) 1142
CciI TCATGA 1 cut(s) 378
Cfr10I RCCGGY 4 cut(s) 310, 601, 1022, 1099
ClaI ATCGAT 1 cut(s) 168
Csp6I GTAC 4 cut(s) 102, 419, 567, 664
CviAII CATG 8 cut(s) 146, 181, 302, 379, 497, 910, 1012, 1375
CviQI GTAC 4 cut(s) 102, 419, 567, 664
DdeI CTNAG 9 cut(s) 65, 159, 321, 458, 552, 594, 965, 1179, 1245
DpnI GATC 8 cut(s) 95, 167, 206, 407, 592, 720, 962, 1306
DpnII GATC 8 cut(s) 93, 165, 204, 405, 590, 718, 960, 1304
Eam1104I CTCTTC 4 cut(s) 79, 374, 944, 1079
EarI CTCTTC 4 cut(s) 79, 374, 944, 1079
Eco130I CCWWGG 4 cut(s) 124, 145, 682, 1374
EcoNI CCTNNNNNAGG 1 cut(s) 686
EcoT14I CCWWGG 4 cut(s) 124, 145, 682, 1374
ErhI CCWWGG 4 cut(s) 124, 145, 682, 1374
FaeI CATG 8 cut(s) 149, 184, 305, 382, 500, 913, 1015, 1378
FaqI GGGAC 2 cut(s) 68, 1253
FatI CATG 8 cut(s) 145, 180, 301, 378, 496, 909, 1011, 1374
FblI GTMKAC 1 cut(s) 1359
Fnu4HI GCNGC 4 cut(s) 642, 975, 1162, 1277
FokI GGATG 3 cut(s) 839, 891, 1378
Fsp4HI GCNGC 4 cut(s) 642, 975, 1162, 1277
FspBI CTAG 3 cut(s) 762, 1175, 1265
GluI GCNGC 4 cut(s) 642, 975, 1162, 1277
GsuI CTGGAG 1 cut(s) 1087
HapII CCGG 5 cut(s) 261, 311, 602, 1023, 1100
Hin1II CATG 8 cut(s) 149, 184, 305, 382, 500, 913, 1015, 1378
HinfI GANTC 6 cut(s) 151, 176, 382, 710, 1071, 1139
HpaII CCGG 5 cut(s) 261, 311, 602, 1023, 1100
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 4 cut(s) 567, 664, 919, 1360
Hpy188I TCNGA 7 cut(s) 162, 219, 324, 461, 540, 595, 1246
Hpy188III TCNNGA 7 cut(s) 208, 261, 379, 824, 1066, 1181, 1395
Hpy8I GTNNAC 4 cut(s) 567, 664, 919, 1360
Hpy99I CGWCG 1 cut(s) 998
HpyAV CCTTC 3 cut(s) 120, 988, 1335
HpyCH4III ACNGT 2 cut(s) 473, 1058
HpyCH4IV ACGT 3 cut(s) 139, 996, 1430
HpyF10VI GCNNNNNNNGC 7 cut(s) 557, 641, 647, 675, 788, 1007, 1167
HpyF3I CTNAG 9 cut(s) 65, 159, 321, 458, 552, 594, 965, 1179, 1245
HpySE526I ACGT 3 cut(s) 139, 996, 1430
Hsp92II CATG 8 cut(s) 149, 184, 305, 382, 500, 913, 1015, 1378
Kpn2I TCCGGA 1 cut(s) 260
KroI GCCGGC 1 cut(s) 1099
KroNI GCCGGC 1 cut(s) 1101
Kzo9I GATC 8 cut(s) 93, 165, 204, 405, 590, 718, 960, 1304
LmnI GCTCC 3 cut(s) 263, 649, 1374
Lsp1109I GCAGC 3 cut(s) 628, 961, 1148
LweI GCATC 1 cut(s) 767
MaeI CTAG 3 cut(s) 762, 1175, 1265
MaeII ACGT 3 cut(s) 139, 996, 1430
MaeIII GTNAC 3 cut(s) 68, 448, 1132
MalI GATC 8 cut(s) 95, 167, 206, 407, 592, 720, 962, 1306
MboI GATC 8 cut(s) 93, 165, 204, 405, 590, 718, 960, 1304
MboII GAAGA 8 cut(s) 96, 105, 175, 361, 931, 1096, 1229, 1421
MfeI CAATTG 1 cut(s) 636
MflI RGATCY 5 cut(s) 93, 405, 590, 960, 1304
MhlI GDGCHC 2 cut(s) 1172, 1251
MluCI AATT 6 cut(s) 541, 636, 746, 785, 1198, 1315
MlyI GAGTC 3 cut(s) 185, 391, 1133
MroI TCCGGA 1 cut(s) 260
MroNI GCCGGC 1 cut(s) 1099
MroXI GAANNNNTTC 1 cut(s) 1088
MseI TTAA 5 cut(s) 296, 345, 428, 573, 1019
MslI CAYNNNNRTG 2 cut(s) 377, 796
MspCI CTTAAG 1 cut(s) 427
MspI CCGG 5 cut(s) 261, 311, 602, 1023, 1100
MunI CAATTG 1 cut(s) 636
Mva1269I GAATGC 2 cut(s) 510, 527
MwoI GCNNNNNNNGC 7 cut(s) 557, 641, 647, 675, 788, 1007, 1167
NaeI GCCGGC 1 cut(s) 1101
NcoI CCATGG 2 cut(s) 145, 1374
NdeII GATC 8 cut(s) 93, 165, 204, 405, 590, 718, 960, 1304
NgoMIV GCCGGC 1 cut(s) 1099
NlaIII CATG 8 cut(s) 149, 184, 305, 382, 500, 913, 1015, 1378
NlaIV GGNNCC 3 cut(s) 407, 962, 1380
NmuCI GTSAC 2 cut(s) 68, 448
NspI RCATGY 1 cut(s) 913
NspV TTCGAA 2 cut(s) 133, 1189
PagI TCATGA 1 cut(s) 378
PaqCI CACCTGC 1 cut(s) 647
PciI ACATGT 1 cut(s) 909
PctI GAATGC 2 cut(s) 510, 527
PdiI GCCGGC 1 cut(s) 1101
PdmI GAANNNNTTC 1 cut(s) 1088
PfeI GAWTC 3 cut(s) 151, 710, 1071
PflFI GACNNNGTC 1 cut(s) 698
PkrI GCNGC 4 cut(s) 643, 976, 1163, 1278
PleI GAGTC 3 cut(s) 184, 390, 1133
PpsI GAGTC 3 cut(s) 184, 390, 1133
PscI ACATGT 1 cut(s) 909
Psp1406I AACGTT 1 cut(s) 139
PspN4I GGNNCC 3 cut(s) 407, 962, 1380
PsrI GAACNNNNNNTAC 2 cut(s) 470, 502
PstI CTGCAG 1 cut(s) 1132
PstNI CAGNNNCTG 1 cut(s) 1142
PsuI RGATCY 5 cut(s) 93, 405, 590, 960, 1304
PsyI GACNNNGTC 1 cut(s) 698
RsaI GTAC 4 cut(s) 103, 420, 568, 665
RsaNI GTAC 4 cut(s) 102, 419, 567, 664
RseI CAYNNNNRTG 2 cut(s) 377, 796
SaqAI TTAA 5 cut(s) 296, 345, 428, 573, 1019
SatI GCNGC 4 cut(s) 642, 975, 1162, 1277
Sau3AI GATC 8 cut(s) 93, 165, 204, 405, 590, 718, 960, 1304
SchI GAGTC 3 cut(s) 185, 391, 1133
SduI GDGCHC 2 cut(s) 1172, 1251
SfaNI GCATC 1 cut(s) 767
SfcI CTRYAG 2 cut(s) 1128, 1204
SfuI TTCGAA 2 cut(s) 133, 1189
SmiMI CAYNNNNRTG 2 cut(s) 377, 796
SmlI CTYRAG 2 cut(s) 97, 427
SmoI CTYRAG 2 cut(s) 97, 427
SpeI ACTAGT 1 cut(s) 1264
Sse9I AATT 6 cut(s) 541, 636, 746, 785, 1198, 1315
SsiI CCGC 2 cut(s) 105, 1276
SspMI CTAG 3 cut(s) 762, 1175, 1265
StyI CCWWGG 4 cut(s) 124, 145, 682, 1374
TaaI ACNGT 2 cut(s) 473, 1058
TaiI ACGT 3 cut(s) 142, 999, 1433
TaqI TCGA 6 cut(s) 40, 133, 168, 702, 1189, 1419
TasI AATT 6 cut(s) 541, 636, 746, 785, 1198, 1315
TatI WGTACW 2 cut(s) 418, 663
TauI GCSGC 1 cut(s) 1279
TfiI GAWTC 3 cut(s) 151, 710, 1071
Tru1I TTAA 5 cut(s) 296, 345, 428, 573, 1019
Tru9I TTAA 5 cut(s) 296, 345, 428, 573, 1019
TseFI GTSAC 2 cut(s) 68, 448
TseI GCWGC 3 cut(s) 641, 974, 1161
Tsp45I GTSAC 2 cut(s) 68, 448
TspDTI ATGAA 2 cut(s) 513, 741
TspGWI ACGGA 2 cut(s) 495, 1302
Tth111I GACNNNGTC 1 cut(s) 698
Vha464I CTTAAG 1 cut(s) 427
XagI CCTNNNNNAGG 1 cut(s) 686
XapI RAATTY 3 cut(s) 746, 785, 1198
XceI RCATGY 1 cut(s) 913
XmiI GTMKAC 1 cut(s) 1359
XmnI GAANNNNTTC 1 cut(s) 1088
XspI CTAG 3 cut(s) 762, 1175, 1265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.