pycom12g17270

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0028703
Physical Location & Seq
Reverse (-)
19617428 .. 19619546
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1593 bp
ATGGCGTGCACTTCATTTACTTTTCCGGCCTCTCCAACCTCCTCAACTCTAAGGCCGAAAGACATCTTCAAAGAGCATATTCGTGTCCGAAGATTTTGCTCAGTTAACCATCCTTGTCATTGCATCAATCATATCCAGGTCTCTCAATCAAAACTACAAAACAACAACCGTTCCATTGGAATTTCCCCGTTTCAAAGTTTGAGTTCCATGGTCCCAAATTTGCTTAGATCATTTTTGTTTGGCCCGGCCACGAGTAAGCATATGAAAATGCAGAATAGCATGCATATAATGGAGAAATTTGGAGAGAGTACTAGTGATCAAGGGGAGGAAATAAAGGGAGCCAATTGGATGGAGGGGCTTGTGGCACTCGGTGACCTGTGGAGCAATATTTGGGAGCAAAAAGAGGTTGCGGACGAAGACAACACTCATGATGAGGGTGAAAACGGTGGTTGTGGAAGTGGTGGAGAGGAAGGTGGTTGTGCGGAGAGTTGTGGTTTAGAAGATAATGATGGGGAAATGAGATACAGATCATACGACCAGGAGTCTTTCTCGAGGTTTTTAGTTAGAGTACCATGGTCAGATACCAAGCTATTTTCTAAGCTAGCCTTCTTGTGCAACATGGCTTGTGTGATACCAGAGATCAAGACTAACGCGCATGCAGGCCGAGGATTTGAAGATATATGGCCTCCAATTTGTAACATCTTCATTACAAAAGAAAGCAGAAGTAGCTGTTTCTTTGGAAAGTTCAACGATGACAGAAGTACTTCAAGCACTCCTCCAATGGTATTTGTTACATCTATGTGGGTCAAATTTTCGAAACAACGAGATGATGTGCCACAGATTGGAAATGCACAGCTGAAAATCGTCCTTCATTTGCAGGGCATGGACTCCCTTGCTTCCCGACAGGCATACCTCTTTTTCGAACCCACCAAATTTGAGGAGACGAATGTACATGTTCACAGAGGAACCTATGAAGCGGCACAGCAAATGTACCAGCAATTCATGCCAGATATATTGGAGCACTTACATAGAAACGGTGAACTCGCAAAGCTTCAATTTACTGGCCATTGTCTTGGAGGAAGCATTGCTCTTTTAGTCCACATGATGCTCCTCACTAGGAAGCTTGTGAAACCCTCTACTCTTCTGCCGGTTGTCACATTTGGATCACCATTTGTGTTCTGCGGAGGCCACAAGTTACTCGATCAACTAGGGTTGGATGTGAATAATCACATCCATTGTGTGATGATGCATAGAGATGTTGTCCCTAGAGCTTTCTCCTGCAACTACCCCAACCACGTGGCTACACTCCTCAAGCGTTTAAATGGTTCATTTCAGTCACTCCCTTGCCTGATAAAAAATGTAAGTACCAAATATTGTTTAACTCATAGAATCCTCCACATTAATAAATCGCTTTTTCTGATGTATATGTTTTTATGCAGAAGACGTTGTACTCTCCAATCGGCAAACTATTCATTCTTCAACCTGATGAAAGGTTATCTCCTCCACACCCTCTACTCCCTATGGGGAGCGCCCTCTCTGTCCTGGACAACACGCAATCTGGTTTCTCTTCAGATATCTTTAGGGCCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

531

Amino Acids

59.99

Weight (kDa)

8.44

Isoelectric Point (pI)

58.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 292 - 428 5.2e-23 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028703)

Species Orthologous Gene IDs
malus_domestica MD04G1170000.v1.1
pyrus_communis pycom12g17270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 842
AccII CGCG 1 cut(s) 653
AciI CCGC 4 cut(s) 410, 482, 977, 1182
AclWI GGATC 1 cut(s) 1171
AcoI YGGCCR 2 cut(s) 246, 1063
AcsI RAATTY 5 cut(s) 180, 217, 296, 809, 932
AcuI CTGAAG 1 cut(s) 1553
AcvI CACGTG 1 cut(s) 1297
AdeI CACNNNGTG 1 cut(s) 371
AfaI GTAC 7 cut(s) 310, 570, 763, 951, 992, 1366, 1450
AfiI CCNNNNNNNGG 1 cut(s) 842
AflIII ACRYGT 1 cut(s) 952
AgsI TTSAA 7 cut(s) 70, 194, 674, 748, 768, 1055, 1480
AhdI GACNNNNNGTC 1 cut(s) 541
AhlI ACTAGT 1 cut(s) 311
AjnI CCWGG 3 cut(s) 135, 537, 1541
AluBI AGCT 7 cut(s) 589, 601, 729, 856, 1051, 1123, 1271
AluI AGCT 7 cut(s) 589, 601, 729, 856, 1051, 1123, 1271
Alw21I GWGCWC 2 cut(s) 11, 1023
Alw26I GTCTC 2 cut(s) 145, 935
Alw44I GTGCAC 1 cut(s) 7
AlwI GGATC 1 cut(s) 1171
Ama87I CYCGRG 1 cut(s) 550
AoxI GGCC 9 cut(s) 27, 53, 241, 246, 661, 683, 1063, 1186, 1583
ApaLI GTGCAC 1 cut(s) 7
ApoI RAATTY 5 cut(s) 180, 217, 296, 809, 932
AseI ATTAAT 1 cut(s) 1401
Asp700I GAANNNNTTC 1 cut(s) 763
AspLEI GCGC 2 cut(s) 655, 1531
AspS9I GGNCC 3 cut(s) 211, 242, 1583
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 4 cut(s) 383, 449, 1049, 1158
AsuII TTCGAA 2 cut(s) 815, 921
AsuNHI GCTAGC 1 cut(s) 601
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 1 cut(s) 211
BaeGI GKGCMC 1 cut(s) 11
BalI TGGCCA 1 cut(s) 1065
BarI GAAGNNNNNNTAC 2 cut(s) 1432, 1464
BauI CACGAG 1 cut(s) 250
BbrPI CACGTG 1 cut(s) 1297
BbsI GAAGAC 2 cut(s) 423, 1447
Bbv12I GWGCWC 2 cut(s) 11, 1023
BccI CCATC 3 cut(s) 117, 343, 503
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciT130I CCWGG 3 cut(s) 137, 539, 1543
BclI TGATCA 1 cut(s) 316
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 2 cut(s) 145, 935
BcuI ACTAGT 1 cut(s) 311
BfaI CTAG 5 cut(s) 312, 602, 1116, 1208, 1266
BfoI RGCGCY 1 cut(s) 1532
BisI GCNGC 1 cut(s) 978
BlsI GCNGC 1 cut(s) 979
BmcAI AGTACT 2 cut(s) 310, 763
Bme1390I CCNGG 4 cut(s) 137, 245, 539, 1543
Bme18I GGWCC 1 cut(s) 211
BmeRI GACNNNNNGTC 1 cut(s) 541
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 3 cut(s) 211, 242, 1583
BmiI GGNNCC 3 cut(s) 213, 340, 967
BmrFI CCNGG 4 cut(s) 137, 245, 539, 1543
BmsI GCATC 3 cut(s) 132, 1095, 1236
BmtI GCTAGC 1 cut(s) 605
BpiI GAAGAC 2 cut(s) 423, 1447
BplI GAGNNNNNCTC 2 cut(s) 533, 565
Bpu14I TTCGAA 2 cut(s) 815, 921
BpuEI CTTGAG 1 cut(s) 1295
BpuMI CCSGG 1 cut(s) 245
BsaAI YACGTR 1 cut(s) 1297
BsaBI GATNNNNATC 1 cut(s) 526
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 3 cut(s) 207, 572, 664
Bsc4I CCNNNNNNNGG 1 cut(s) 842
Bse118I RCCGGY 1 cut(s) 1147
Bse1I ACTGG 1 cut(s) 1066
Bse3DI GCAATG 2 cut(s) 118, 1083
Bse8I GATNNNNATC 1 cut(s) 526
BseBI CCWGG 3 cut(s) 137, 539, 1543
BseDI CCNNGG 3 cut(s) 207, 572, 664
BseGI GGATG 4 cut(s) 109, 354, 1222, 1230
BseJI GATNNNNATC 1 cut(s) 526
BseLI CCNNNNNNNGG 1 cut(s) 842
BseMI GCAATG 2 cut(s) 118, 1083
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 1066
BseRI GAGGAG 6 cut(s) 31, 765, 953, 1100, 1298, 1490
BseSI GKGCMC 1 cut(s) 11
Bsh1236I CGCG 1 cut(s) 653
BshFI GGCC 9 cut(s) 29, 55, 243, 248, 663, 685, 1065, 1188, 1585
BsiHKAI GWGCWC 2 cut(s) 11, 1023
BsiHKCI CYCGRG 1 cut(s) 550
BsiSI CCGG 3 cut(s) 26, 245, 1148
BslFI GGGAC 2 cut(s) 197, 1247
BslI CCNNNNNNNGG 1 cut(s) 842
BsmAI GTCTC 2 cut(s) 145, 935
BsmBI CGTCTC 1 cut(s) 935
BsmFI GGGAC 2 cut(s) 197, 1247
BsnI GGCC 9 cut(s) 29, 55, 243, 248, 663, 685, 1065, 1188, 1585
Bso31I GGTCTC 1 cut(s) 145
BsoBI CYCGRG 1 cut(s) 550
Bsp119I TTCGAA 2 cut(s) 815, 921
Bsp1286I GDGCHC 2 cut(s) 11, 1023
Bsp1407I TGTACA 1 cut(s) 949
Bsp143I GATC 6 cut(s) 227, 316, 527, 639, 1163, 1201
Bsp19I CCATGG 2 cut(s) 207, 572
BspACI CCGC 4 cut(s) 410, 482, 977, 1182
BspANI GGCC 9 cut(s) 29, 55, 243, 248, 663, 685, 1065, 1188, 1585
BspCNI CTCAG 1 cut(s) 113
BspFNI CGCG 1 cut(s) 653
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 3 cut(s) 213, 340, 967
BspOI GCTAGC 1 cut(s) 605
BspPI GGATC 1 cut(s) 1171
BspT104I TTCGAA 2 cut(s) 815, 921
BspTNI GGTCTC 1 cut(s) 145
BsrDI GCAATG 2 cut(s) 118, 1083
BsrFI RCCGGY 1 cut(s) 1147
BsrGI TGTACA 1 cut(s) 949
BsrI ACTGG 1 cut(s) 1066
BssAI RCCGGY 1 cut(s) 1147
BssECI CCNNGG 3 cut(s) 207, 572, 664
BssMI GATC 6 cut(s) 227, 316, 527, 639, 1163, 1201
BssSI CACGAG 1 cut(s) 250
BssT1I CCWWGG 2 cut(s) 207, 572
Bst2BI CACGAG 1 cut(s) 250
Bst2UI CCWGG 3 cut(s) 137, 539, 1543
Bst4CI ACNGT 3 cut(s) 170, 446, 1037
Bst6I CTCTTC 2 cut(s) 1146, 1572
BstAPI GCANNNNNTGC 1 cut(s) 1003
BstAUI TGTACA 1 cut(s) 949
BstBAI YACGTR 1 cut(s) 1297
BstBI TTCGAA 2 cut(s) 815, 921
BstC8I GCNNGC 5 cut(s) 7, 281, 603, 657, 661
BstDEI CTNAG 4 cut(s) 50, 100, 224, 597
BstDSI CCRYGG 2 cut(s) 207, 572
BstEII GGTNACC 1 cut(s) 371
BstF5I GGATG 4 cut(s) 109, 354, 1222, 1230
BstFNI CGCG 1 cut(s) 653
BstH2I RGCGCY 1 cut(s) 1532
BstHHI GCGC 2 cut(s) 655, 1531
BstKTI GATC 6 cut(s) 230, 319, 530, 642, 1166, 1204
BstMAI GTCTC 2 cut(s) 145, 935
BstMBI GATC 6 cut(s) 227, 316, 527, 639, 1163, 1201
BstMWI GCNNNNNNNGC 2 cut(s) 726, 1003
BstNI CCWGG 3 cut(s) 137, 539, 1543
BstNSI RCATGY 3 cut(s) 283, 659, 956
BstPI GGTNACC 1 cut(s) 371
BstSCI CCNGG 4 cut(s) 135, 243, 537, 1541
BstSLI GKGCMC 1 cut(s) 11
BstUI CGCG 1 cut(s) 653
BstV2I GAAGAC 2 cut(s) 423, 1447
BstXI CCANNNNNNTGG 3 cut(s) 349, 1073, 1297
BsuRI GGCC 9 cut(s) 29, 55, 243, 248, 663, 685, 1065, 1188, 1585
BtgI CCRYGG 2 cut(s) 207, 572
BtsCI GGATG 4 cut(s) 109, 354, 1222, 1230
Cac8I GCNNGC 5 cut(s) 7, 281, 603, 657, 661
CciI TCATGA 1 cut(s) 427
CfoI GCGC 2 cut(s) 655, 1531
Cfr10I RCCGGY 1 cut(s) 1147
Cfr13I GGNCC 3 cut(s) 211, 242, 1583
Csp6I GTAC 7 cut(s) 309, 569, 762, 950, 991, 1365, 1449
CspCI CAANNNNNGTGG 2 cut(s) 916, 951
CviQI GTAC 7 cut(s) 309, 569, 762, 950, 991, 1365, 1449
DdeI CTNAG 4 cut(s) 50, 100, 224, 597
DpnI GATC 6 cut(s) 229, 318, 529, 641, 1165, 1203
DpnII GATC 6 cut(s) 227, 316, 527, 639, 1163, 1201
DraI TTTAAA 1 cut(s) 1320
DraIII CACNNNGTG 1 cut(s) 371
DriI GACNNNNNGTC 1 cut(s) 541
EaeI YGGCCR 2 cut(s) 246, 1063
Eam1104I CTCTTC 2 cut(s) 1146, 1572
Eam1105I GACNNNNNGTC 1 cut(s) 541
EarI CTCTTC 2 cut(s) 1146, 1572
Eco130I CCWWGG 2 cut(s) 207, 572
Eco31I GGTCTC 1 cut(s) 145
Eco32I GATATC 1 cut(s) 1575
Eco47I GGWCC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 1553
Eco72I CACGTG 1 cut(s) 1297
Eco88I CYCGRG 1 cut(s) 550
Eco91I GGTNACC 1 cut(s) 371
EcoO109I RGGNCCY 1 cut(s) 1583
EcoO65I GGTNACC 1 cut(s) 371
EcoRII CCWGG 3 cut(s) 135, 537, 1541
EcoRV GATATC 1 cut(s) 1575
EcoT14I CCWWGG 2 cut(s) 207, 572
EcoT22I ATGCAT 2 cut(s) 285, 1251
ErhI CCWWGG 2 cut(s) 207, 572
Esp3I CGTCTC 1 cut(s) 935
FalI AAGNNNNNCTT 2 cut(s) 590, 622
FaqI GGGAC 2 cut(s) 197, 1247
FauNDI CATATG 1 cut(s) 261
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 1 cut(s) 978
FokI GGATG 4 cut(s) 96, 361, 1217, 1229
Fsp4HI GCNGC 1 cut(s) 978
FspBI CTAG 5 cut(s) 312, 602, 1116, 1208, 1266
GlaI GCGC 2 cut(s) 654, 1530
GluI GCNGC 1 cut(s) 978
HaeII RGCGCY 1 cut(s) 1532
HaeIII GGCC 9 cut(s) 29, 55, 243, 248, 663, 685, 1065, 1188, 1585
HapII CCGG 3 cut(s) 26, 245, 1148
HhaI GCGC 2 cut(s) 655, 1531
Hin6I GCGC 2 cut(s) 653, 1529
HinP1I GCGC 2 cut(s) 653, 1529
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 2 cut(s) 1049, 1121
HinfI GANTC 3 cut(s) 542, 887, 1389
HpaI GTTAAC 1 cut(s) 106
HpaII CCGG 3 cut(s) 26, 245, 1148
HphI GGTGA 4 cut(s) 383, 449, 1049, 1158
Hpy166II GTNNAC 5 cut(s) 9, 106, 958, 1040, 1099
Hpy188I TCNGA 4 cut(s) 89, 580, 1419, 1572
Hpy188III TCNNGA 5 cut(s) 428, 550, 643, 900, 1590
Hpy8I GTNNAC 5 cut(s) 9, 106, 958, 1040, 1099
HpyAV CCTTC 3 cut(s) 464, 616, 878
HpyCH4III ACNGT 3 cut(s) 170, 446, 1037
HpyCH4IV ACGT 2 cut(s) 1296, 1444
HpyF10VI GCNNNNNNNGC 2 cut(s) 726, 1003
HpyF3I CTNAG 4 cut(s) 50, 100, 224, 597
HpySE526I ACGT 2 cut(s) 1296, 1444
HspAI GCGC 2 cut(s) 653, 1529
Ksp22I TGATCA 1 cut(s) 316
KspAI GTTAAC 1 cut(s) 106
Kzo9I GATC 6 cut(s) 227, 316, 527, 639, 1163, 1201
LmnI GCTCC 6 cut(s) 338, 381, 394, 1018, 1113, 1526
LweI GCATC 3 cut(s) 132, 1095, 1236
MaeI CTAG 5 cut(s) 312, 602, 1116, 1208, 1266
MaeII ACGT 2 cut(s) 1296, 1444
MaeIII GTNAC 6 cut(s) 371, 695, 790, 1153, 1194, 1335
MalI GATC 6 cut(s) 229, 318, 529, 641, 1165, 1203
MboI GATC 6 cut(s) 227, 316, 527, 639, 1163, 1201
MfeI CAATTG 1 cut(s) 343
MhlI GDGCHC 2 cut(s) 11, 1023
MlsI TGGCCA 1 cut(s) 1065
MluCI AATT 9 cut(s) 180, 217, 296, 343, 690, 809, 932, 998, 1055
MluNI TGGCCA 1 cut(s) 1065
MlyI GAGTC 2 cut(s) 551, 881
MmeI TCCRAC 2 cut(s) 59, 1194
Mox20I TGGCCA 1 cut(s) 1065
Mph1103I ATGCAT 2 cut(s) 285, 1251
MroXI GAANNNNTTC 1 cut(s) 763
MscI TGGCCA 1 cut(s) 1065
MseI TTAA 4 cut(s) 105, 1319, 1379, 1401
MslI CAYNNNNRTG 3 cut(s) 81, 799, 1254
Msp20I TGGCCA 1 cut(s) 1065
MspA1I CMGCKG 1 cut(s) 856
MspI CCGG 3 cut(s) 26, 245, 1148
MspR9I CCNGG 4 cut(s) 137, 245, 539, 1543
MunI CAATTG 1 cut(s) 343
MvaI CCWGG 3 cut(s) 137, 539, 1543
MvnI CGCG 1 cut(s) 653
MwoI GCNNNNNNNGC 2 cut(s) 726, 1003
NciI CCSGG 1 cut(s) 245
NcoI CCATGG 2 cut(s) 207, 572
NdeI CATATG 1 cut(s) 261
NdeII GATC 6 cut(s) 227, 316, 527, 639, 1163, 1201
NheI GCTAGC 1 cut(s) 601
NlaIV GGNNCC 3 cut(s) 213, 340, 967
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 3 cut(s) 371, 1153, 1335
NsiI ATGCAT 2 cut(s) 285, 1251
NspI RCATGY 3 cut(s) 283, 659, 956
NspV TTCGAA 2 cut(s) 815, 921
PaeI GCATGC 2 cut(s) 283, 659
PaeR7I CTCGAG 1 cut(s) 550
PagI TCATGA 1 cut(s) 427
PciI ACATGT 1 cut(s) 952
PdmI GAANNNNTTC 1 cut(s) 763
PfeI GAWTC 1 cut(s) 1389
PflMI CCANNNNNTGG 1 cut(s) 842
PfoI TCCNGGA 1 cut(s) 1541
PkrI GCNGC 1 cut(s) 979
PleI GAGTC 2 cut(s) 550, 881
PmaCI CACGTG 1 cut(s) 1297
PmlI CACGTG 1 cut(s) 1297
PpsI GAGTC 2 cut(s) 550, 881
Ppu21I YACGTR 1 cut(s) 1297
PscI ACATGT 1 cut(s) 952
PshBI ATTAAT 1 cut(s) 1401
Psp6I CCWGG 3 cut(s) 135, 537, 1541
PspCI CACGTG 1 cut(s) 1297
PspEI GGTNACC 1 cut(s) 371
PspGI CCWGG 3 cut(s) 135, 537, 1541
PspN4I GGNNCC 3 cut(s) 213, 340, 967
PspPI GGNCC 3 cut(s) 211, 242, 1583
PvuII CAGCTG 1 cut(s) 856
RsaI GTAC 7 cut(s) 310, 570, 763, 951, 992, 1366, 1450
RsaNI GTAC 7 cut(s) 309, 569, 762, 950, 991, 1365, 1449
RseI CAYNNNNRTG 3 cut(s) 81, 799, 1254
SaqAI TTAA 4 cut(s) 105, 1319, 1379, 1401
SatI GCNGC 1 cut(s) 978
Sau3AI GATC 6 cut(s) 227, 316, 527, 639, 1163, 1201
Sau96I GGNCC 3 cut(s) 211, 242, 1583
ScaI AGTACT 2 cut(s) 310, 763
SchI GAGTC 2 cut(s) 551, 881
ScrFI CCNGG 4 cut(s) 137, 245, 539, 1543
SduI GDGCHC 2 cut(s) 11, 1023
SfaNI GCATC 3 cut(s) 132, 1095, 1236
Sfr274I CTCGAG 1 cut(s) 550
SfuI TTCGAA 2 cut(s) 815, 921
SinI GGWCC 1 cut(s) 211
SlaI CTCGAG 1 cut(s) 550
SmiMI CAYNNNNRTG 3 cut(s) 81, 799, 1254
SmlI CTYRAG 2 cut(s) 550, 1310
SmoI CTYRAG 2 cut(s) 550, 1310
SpeI ACTAGT 1 cut(s) 311
SphI GCATGC 2 cut(s) 283, 659
Sse9I AATT 9 cut(s) 180, 217, 296, 343, 690, 809, 932, 998, 1055
SsiI CCGC 4 cut(s) 410, 482, 977, 1182
SspI AATATT 2 cut(s) 388, 1373
SspMI CTAG 5 cut(s) 312, 602, 1116, 1208, 1266
StyD4I CCNGG 4 cut(s) 135, 243, 537, 1541
StyI CCWWGG 2 cut(s) 207, 572
TaaI ACNGT 3 cut(s) 170, 446, 1037
TaiI ACGT 2 cut(s) 1299, 1447
TaqI TCGA 4 cut(s) 551, 815, 921, 1200
TasI AATT 9 cut(s) 180, 217, 296, 343, 690, 809, 932, 998, 1055
TatI WGTACW 4 cut(s) 308, 761, 949, 1448
TauI GCSGC 1 cut(s) 980
TfiI GAWTC 1 cut(s) 1389
Tru1I TTAA 4 cut(s) 105, 1319, 1379, 1401
Tru9I TTAA 4 cut(s) 105, 1319, 1379, 1401
TseFI GTSAC 3 cut(s) 371, 1153, 1335
Tsp45I GTSAC 3 cut(s) 371, 1153, 1335
TspDTI ATGAA 8 cut(s) 278, 694, 860, 987, 991, 1317, 1461, 1502
Van91I CCANNNNNTGG 1 cut(s) 842
VneI GTGCAC 1 cut(s) 7
VpaK11BI GGWCC 1 cut(s) 211
VspI ATTAAT 1 cut(s) 1401
XapI RAATTY 5 cut(s) 180, 217, 296, 809, 932
XceI RCATGY 3 cut(s) 283, 659, 956
XhoI CTCGAG 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 763
XspI CTAG 5 cut(s) 312, 602, 1116, 1208, 1266
ZrmI AGTACT 2 cut(s) 310, 763
Zsp2I ATGCAT 2 cut(s) 285, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.