pycom12g20330

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0024428
Physical Location & Seq
Reverse (-)
21889065 .. 21891210
2146 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1380 bp
ATGGAGACTTGTAGGGCAAGTAAGAGTAGTGAAACAGTTCCGAGAGTTTGTCTAGAGTTTAGGGTGGATAGGCTGAGTAGTCTTCCAGACGAAATTACTCATGGAATCCTCTCGTTGCTTCCATTTGGAGACCTCTCTCGCTTTGGGAGTTTATCCAAACGCTGCAGGGAGCTCCTTTGGTCAACTCCCAGTTTCAGATTAGATTTGTCTAAAGTTGATATATCAACTTGTGATAAGTGGTCCAGGTTCGCAAATTCTGTGGATAGGTTCTTAGACCATCGTGGGGAGAACAGAATCCTTCACTTAGGTATTAGATTCGACGTCCGCGAACATATTTTTGGAAATGAAACCCCATGCATTTGTGAGGGGGTAGGACGTCGGTTTGTTTCATGGATCGAAAATGCTTTAAGGTGCAAGGTTGAAATACTTGACCTTGATATCAGTAGGATCCATCTAGCGAATGGGGTAGGTGATATCACCATTGAGAGCTCATCACTAAAATCACTCAGTATTTCGAGCAACAGTTGGACAGTTCGTCGACTGAAAATCATCGGTCATAAGCTTGAAGATGTTCGCATAGATTGGCGTTCGGAGTGGTGTACAACAGTAAGCATATTTGCTTCAGATGTTGAGAATTTGGAGTGGGATGGGATTACTGTCAACCACCAGCTCGGGACCTTAGAAAGGGCCGAGCTTTCTTTGGCCGATGTCATACGTAGAGATCTGTCACCTGATGTGAAGGCAGTTGACTTCCTTACCAGCGTGCACCGGGTTAAAGCTTTAACTCTAAATCACAACACCATCAAGGTAAAGACACTACTCTTCTGGCAAAGTCTTACTATGGAATACTGGAGGTCAAGCAAGCTTCCTTTCCTCTCTCAGCTTAAGGAGGTAACCGTAGCCCTTAATCGACACTTCTACGGGACCAACCAAATCGAGTTCATAAGTCTAGTGCAGATTCACAAGCCCCACCTGCTCAGCCTCCAAGTTAAGAGGGTCTTGAGGGCTTTTGGGGATATGGGTTTTGACAAAGGCGAAGTGGGGTTGGGGGAGATTCGGGATTTCCAAGTTGAAGATCCAAGAAAAGAGCAAACCGCAGATTCGGGTGTTCTTCTTTTCCTTGAGTGTCTCTCTGTTTGCAGGATTCCTGCACTCGGTCCTCGCATGCCGGATAGGAGGACACTGAAATGCTGCAGGCTTGCACTGAGAATTCCCCATATGCAGGACCATTCCGAAATAATGCCCCATCATTGTGCCATGCCTTTTGAACCCAAAGGAACTTTAAGCTCTATATCTGCAATTCTCTCCATGATTATGGGAGTGCATTTCGGACATGTGCTTATACATATGAAGGGTCATTCAGCCAGACTGAACCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

460

Amino Acids

52.36

Weight (kDa)

8.53

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024428)

Species Orthologous Gene IDs
malus_domestica MD12G1217700.v1.1 MD12G1217800.v1.1
pyrus_communis pycom12g20330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 981
AatII GACGTC 2 cut(s) 324, 379
Acc36I ACCTGC 1 cut(s) 981
AccI GTMKAC 1 cut(s) 538
AccII CGCG 1 cut(s) 327
AciI CCGC 2 cut(s) 325, 1095
AclWI GGATC 4 cut(s) 401, 442, 455, 1070
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 3 cut(s) 253, 634, 1209
AcuI CTGAAG 1 cut(s) 606
AcyI GRCGYC 2 cut(s) 321, 376
AfaI GTAC 1 cut(s) 601
AfiI CCNNNNNNNGG 4 cut(s) 283, 684, 1154, 1222
AflII CTTAAG 1 cut(s) 884
AflIII ACRYGT 1 cut(s) 1333
AgsI TTSAA 4 cut(s) 422, 566, 1073, 1268
AhdI GACNNNNNGTC 1 cut(s) 534
AjnI CCWGG 1 cut(s) 242
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 9 cut(s) 172, 489, 562, 670, 694, 779, 865, 883, 1287
AluI AGCT 9 cut(s) 172, 489, 562, 670, 694, 779, 865, 883, 1287
Alw21I GWGCWC 3 cut(s) 174, 491, 768
Alw26I GTCTC 2 cut(s) 123, 1133
Alw44I GTGCAC 1 cut(s) 764
AlwI GGATC 4 cut(s) 401, 442, 455, 1070
Ama87I CYCGRG 1 cut(s) 671
AoxI GGCC 2 cut(s) 687, 702
ApaLI GTGCAC 1 cut(s) 764
ApeKI GCWGC 2 cut(s) 162, 1191
ApoI RAATTY 3 cut(s) 253, 634, 1209
Asp700I GAANNNNTTC 2 cut(s) 36, 570
AspS9I GGNCC 6 cut(s) 240, 675, 687, 924, 1157, 1225
AsuC2I CCSGG 1 cut(s) 770
AsuHPI GGTGA 3 cut(s) 469, 482, 720
AvaI CYCGRG 1 cut(s) 671
AvaII GGWCC 5 cut(s) 240, 675, 924, 1157, 1225
BaeGI GKGCMC 1 cut(s) 768
BamHI GGATCC 1 cut(s) 447
BanII GRGCYC 2 cut(s) 174, 491
BbsI GAAGAC 1 cut(s) 74
Bbv12I GWGCWC 3 cut(s) 174, 491, 768
BbvI GCAGC 2 cut(s) 149, 1178
BccI CCATC 5 cut(s) 285, 459, 641, 809, 1254
BciT130I CCWGG 1 cut(s) 244
BcnI CCSGG 1 cut(s) 770
BcoDI GTCTC 2 cut(s) 123, 1133
BfaI CTAG 3 cut(s) 53, 455, 950
BfmI CTRYAG 2 cut(s) 163, 1192
BfrI CTTAAG 1 cut(s) 884
BfuAI ACCTGC 1 cut(s) 981
BglII AGATCT 1 cut(s) 721
BisI GCNGC 2 cut(s) 163, 1192
BlpI GCTNAGC 1 cut(s) 977
BlsI GCNGC 2 cut(s) 164, 1193
Bme1390I CCNGG 2 cut(s) 244, 770
Bme18I GGWCC 5 cut(s) 240, 675, 924, 1157, 1225
BmeRI GACNNNNNGTC 1 cut(s) 534
BmeT110I CYCGRG 1 cut(s) 671
BmgT120I GGNCC 6 cut(s) 240, 675, 687, 924, 1157, 1225
BmiI GGNNCC 3 cut(s) 449, 676, 925
BmrFI CCNGG 2 cut(s) 244, 770
BmrI ACTGGG 1 cut(s) 183
BmuI ACTGGG 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 74
BplI GAGNNNNNCTC 2 cut(s) 1115, 1147
BpmI CTGGAG 1 cut(s) 871
Bpu1102I GCTNAGC 1 cut(s) 977
BpuEI CTTGAG 2 cut(s) 1021, 1142
BpuMI CCSGG 1 cut(s) 770
BsaAI YACGTR 1 cut(s) 716
BsaHI GRCGYC 2 cut(s) 321, 376
BsaI GGTCTC 1 cut(s) 123
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 684, 1154, 1222
Bse1I ACTGG 2 cut(s) 189, 854
BseBI CCWGG 1 cut(s) 244
BseGI GGATG 1 cut(s) 652
BseLI CCNNNNNNNGG 4 cut(s) 283, 684, 1154, 1222
BseMII CTCAG 5 cut(s) 65, 520, 893, 991, 1196
BseNI ACTGG 2 cut(s) 189, 854
BseSI GKGCMC 1 cut(s) 768
BseXI GCAGC 2 cut(s) 149, 1178
BsgI GTGCAG 2 cut(s) 974, 1134
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 2 cut(s) 689, 704
BsiHKAI GWGCWC 3 cut(s) 174, 491, 768
BsiHKCI CYCGRG 1 cut(s) 671
BsiSI CCGG 2 cut(s) 769, 1169
BslFI GGGAC 2 cut(s) 688, 937
BslI CCNNNNNNNGG 4 cut(s) 283, 684, 1154, 1222
BsmAI GTCTC 2 cut(s) 123, 1133
BsmFI GGGAC 2 cut(s) 688, 937
BsnI GGCC 2 cut(s) 689, 704
Bso31I GGTCTC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 671
Bsp1286I GDGCHC 3 cut(s) 174, 491, 768
Bsp1407I TGTACA 1 cut(s) 599
Bsp143I GATC 4 cut(s) 393, 447, 721, 1075
Bsp1720I GCTNAGC 1 cut(s) 977
BspACI CCGC 2 cut(s) 325, 1095
BspANI GGCC 2 cut(s) 689, 704
BspCNI CTCAG 5 cut(s) 66, 519, 892, 990, 1197
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 3 cut(s) 449, 676, 925
BspMAI CTGCAG 2 cut(s) 167, 1196
BspMI ACCTGC 1 cut(s) 981
BspPI GGATC 4 cut(s) 401, 442, 455, 1070
BspTI CTTAAG 1 cut(s) 884
BspTNI GGTCTC 1 cut(s) 123
BsrGI TGTACA 1 cut(s) 599
BsrI ACTGG 2 cut(s) 189, 854
BssMI GATC 4 cut(s) 393, 447, 721, 1075
BssNI GRCGYC 2 cut(s) 321, 376
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 6 cut(s) 37, 524, 532, 607, 658, 898
Bst6I CTCTTC 1 cut(s) 827
BstACI GRCGYC 2 cut(s) 321, 376
BstAFI CTTAAG 1 cut(s) 884
BstAUI TGTACA 1 cut(s) 599
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 5 cut(s) 764, 863, 1166, 1196, 1200
BstDEI CTNAG 8 cut(s) 74, 271, 304, 506, 679, 879, 977, 1205
BstEII GGTNACC 1 cut(s) 892
BstENI CCTNNNNNAGG 1 cut(s) 682
BstF5I GGATG 1 cut(s) 652
BstFNI CGCG 1 cut(s) 327
BstKTI GATC 4 cut(s) 396, 450, 724, 1078
BstMAI GTCTC 2 cut(s) 123, 1133
BstMBI GATC 4 cut(s) 393, 447, 721, 1075
BstMWI GCNNNNNNNGC 1 cut(s) 973
BstNI CCWGG 1 cut(s) 244
BstNSI RCATGY 2 cut(s) 1168, 1337
BstPI GGTNACC 1 cut(s) 892
BstSCI CCNGG 2 cut(s) 242, 768
BstSFI CTRYAG 2 cut(s) 163, 1192
BstSLI GKGCMC 1 cut(s) 768
BstSNI TACGTA 1 cut(s) 716
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 2 cut(s) 149, 1178
BstV2I GAAGAC 1 cut(s) 74
BstX2I RGATCY 3 cut(s) 447, 721, 1075
BstXI CCANNNNNNTGG 1 cut(s) 1315
BstYI RGATCY 3 cut(s) 447, 721, 1075
BsuRI GGCC 2 cut(s) 689, 704
BtsCI GGATG 1 cut(s) 652
BtsIMutI CAGTG 2 cut(s) 1181, 1202
BveI ACCTGC 1 cut(s) 981
Cac8I GCNNGC 5 cut(s) 764, 863, 1166, 1196, 1200
Cfr13I GGNCC 6 cut(s) 240, 675, 687, 924, 1157, 1225
Csp6I GTAC 1 cut(s) 600
CspCI CAANNNNNGTGG 2 cut(s) 240, 275
CviAII CATG 7 cut(s) 101, 354, 390, 1165, 1258, 1309, 1334
CviQI GTAC 1 cut(s) 600
DdeI CTNAG 8 cut(s) 74, 271, 304, 506, 679, 879, 977, 1205
DpnI GATC 4 cut(s) 395, 449, 723, 1077
DpnII GATC 4 cut(s) 393, 447, 721, 1075
DriI GACNNNNNGTC 1 cut(s) 534
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 1 cut(s) 827
Eam1105I GACNNNNNGTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 827
Ecl136II GAGCTC 2 cut(s) 172, 489
Eco105I TACGTA 1 cut(s) 716
Eco24I GRGCYC 2 cut(s) 174, 491
Eco31I GGTCTC 1 cut(s) 123
Eco32I GATATC 2 cut(s) 439, 475
Eco47I GGWCC 5 cut(s) 240, 675, 924, 1157, 1225
Eco53kI GAGCTC 2 cut(s) 172, 489
Eco57I CTGAAG 1 cut(s) 606
Eco88I CYCGRG 1 cut(s) 671
Eco91I GGTNACC 1 cut(s) 892
EcoICRI GAGCTC 2 cut(s) 172, 489
EcoNI CCTNNNNNAGG 1 cut(s) 682
EcoO109I RGGNCCY 1 cut(s) 675
EcoO65I GGTNACC 1 cut(s) 892
EcoRI GAATTC 1 cut(s) 1209
EcoRII CCWGG 1 cut(s) 242
EcoRV GATATC 2 cut(s) 439, 475
EcoT22I ATGCAT 1 cut(s) 359
EcoT38I GRGCYC 2 cut(s) 174, 491
FaeI CATG 7 cut(s) 104, 357, 393, 1168, 1261, 1312, 1337
FalI AAGNNNNNCTT 2 cut(s) 983, 1015
FaqI GGGAC 2 cut(s) 688, 937
FatI CATG 7 cut(s) 100, 353, 389, 1164, 1257, 1308, 1333
FauNDI CATATG 2 cut(s) 1218, 1347
FblI GTMKAC 1 cut(s) 538
Fnu4HI GCNGC 2 cut(s) 163, 1192
FokI GGATG 1 cut(s) 659
FriOI GRGCYC 2 cut(s) 174, 491
Fsp4HI GCNGC 2 cut(s) 163, 1192
FspBI CTAG 3 cut(s) 53, 455, 950
GluI GCNGC 2 cut(s) 163, 1192
GsuI CTGGAG 1 cut(s) 871
HaeIII GGCC 2 cut(s) 689, 704
HapII CCGG 2 cut(s) 769, 1169
Hin1I GRCGYC 2 cut(s) 321, 376
Hin1II CATG 7 cut(s) 104, 357, 393, 1168, 1261, 1312, 1337
HincII GTYRAC 4 cut(s) 183, 539, 661, 748
HindII GTYRAC 4 cut(s) 183, 539, 661, 748
HindIII AAGCTT 3 cut(s) 560, 777, 863
HinfI GANTC 7 cut(s) 105, 294, 315, 958, 1054, 1100, 1144
HpaII CCGG 2 cut(s) 769, 1169
HphI GGTGA 3 cut(s) 469, 482, 720
Hpy166II GTNNAC 6 cut(s) 183, 539, 600, 661, 748, 766
Hpy188I TCNGA 6 cut(s) 42, 197, 592, 625, 1234, 1331
Hpy188III TCNNGA 5 cut(s) 53, 86, 673, 1000, 1058
Hpy8I GTNNAC 6 cut(s) 183, 539, 600, 661, 748, 766
Hpy99I CGWCG 3 cut(s) 323, 381, 540
HpyAV CCTTC 3 cut(s) 308, 733, 1345
HpyCH4III ACNGT 6 cut(s) 37, 524, 532, 607, 658, 898
HpyCH4IV ACGT 3 cut(s) 321, 376, 715
HpyF10VI GCNNNNNNNGC 1 cut(s) 973
HpyF3I CTNAG 8 cut(s) 74, 271, 304, 506, 679, 879, 977, 1205
HpySE526I ACGT 3 cut(s) 321, 376, 715
Hsp92I GRCGYC 2 cut(s) 321, 376
Hsp92II CATG 7 cut(s) 104, 357, 393, 1168, 1261, 1312, 1337
Kzo9I GATC 4 cut(s) 393, 447, 721, 1075
LmnI GCTCC 2 cut(s) 169, 177
Lsp1109I GCAGC 2 cut(s) 149, 1178
MaeI CTAG 3 cut(s) 53, 455, 950
MaeII ACGT 3 cut(s) 321, 376, 715
MaeIII GTNAC 2 cut(s) 726, 892
MalI GATC 4 cut(s) 395, 449, 723, 1077
MboI GATC 4 cut(s) 393, 447, 721, 1075
MboII GAAGA 5 cut(s) 74, 578, 814, 1085, 1103
MflI RGATCY 3 cut(s) 447, 721, 1075
MhlI GDGCHC 3 cut(s) 174, 491, 768
MluCI AATT 5 cut(s) 93, 253, 634, 1209, 1299
MmeI TCCRAC 1 cut(s) 506
Mph1103I ATGCAT 1 cut(s) 359
MroXI GAANNNNTTC 2 cut(s) 36, 570
MseI TTAA 7 cut(s) 407, 774, 782, 885, 906, 990, 1283
MslI CAYNNNNRTG 3 cut(s) 1186, 1251, 1313
MspCI CTTAAG 1 cut(s) 884
MspI CCGG 2 cut(s) 769, 1169
MspR9I CCNGG 2 cut(s) 244, 770
MvaI CCWGG 1 cut(s) 244
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 1 cut(s) 973
NciI CCSGG 1 cut(s) 770
NdeI CATATG 2 cut(s) 1218, 1347
NdeII GATC 4 cut(s) 393, 447, 721, 1075
NlaIII CATG 7 cut(s) 104, 357, 393, 1168, 1261, 1312, 1337
NlaIV GGNNCC 3 cut(s) 449, 676, 925
NmeAIII GCCGAG 1 cut(s) 715
NmuCI GTSAC 1 cut(s) 726
NsiI ATGCAT 1 cut(s) 359
NspI RCATGY 2 cut(s) 1168, 1337
PaeI GCATGC 1 cut(s) 1168
PaqCI CACCTGC 1 cut(s) 981
PciI ACATGT 1 cut(s) 1333
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PdmI GAANNNNTTC 2 cut(s) 36, 570
PfeI GAWTC 7 cut(s) 105, 294, 315, 958, 1054, 1100, 1144
PkrI GCNGC 2 cut(s) 164, 1193
Ppu21I YACGTR 1 cut(s) 716
PpuMI RGGWCCY 1 cut(s) 675
PscI ACATGT 1 cut(s) 1333
Psp124BI GAGCTC 2 cut(s) 174, 491
Psp5II RGGWCCY 1 cut(s) 675
Psp6I CCWGG 1 cut(s) 242
PspEI GGTNACC 1 cut(s) 892
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 3 cut(s) 449, 676, 925
PspPI GGNCC 6 cut(s) 240, 675, 687, 924, 1157, 1225
PspPPI RGGWCCY 1 cut(s) 675
PstI CTGCAG 2 cut(s) 167, 1196
PsuI RGATCY 3 cut(s) 447, 721, 1075
RsaI GTAC 1 cut(s) 601
RsaNI GTAC 1 cut(s) 600
RseI CAYNNNNRTG 3 cut(s) 1186, 1251, 1313
SacI GAGCTC 2 cut(s) 174, 491
SalI GTCGAC 1 cut(s) 537
SaqAI TTAA 7 cut(s) 407, 774, 782, 885, 906, 990, 1283
SatI GCNGC 2 cut(s) 163, 1192
Sau3AI GATC 4 cut(s) 393, 447, 721, 1075
Sau96I GGNCC 6 cut(s) 240, 675, 687, 924, 1157, 1225
ScrFI CCNGG 2 cut(s) 244, 770
SduI GDGCHC 3 cut(s) 174, 491, 768
SfcI CTRYAG 2 cut(s) 163, 1192
SinI GGWCC 5 cut(s) 240, 675, 924, 1157, 1225
SmiMI CAYNNNNRTG 3 cut(s) 1186, 1251, 1313
SmlI CTYRAG 3 cut(s) 884, 1000, 1121
SmoI CTYRAG 3 cut(s) 884, 1000, 1121
SnaBI TACGTA 1 cut(s) 716
SphI GCATGC 1 cut(s) 1168
Sse9I AATT 5 cut(s) 93, 253, 634, 1209, 1299
SsiI CCGC 2 cut(s) 325, 1095
SspMI CTAG 3 cut(s) 53, 455, 950
SstI GAGCTC 2 cut(s) 174, 491
StyD4I CCNGG 2 cut(s) 242, 768
TaaI ACNGT 6 cut(s) 37, 524, 532, 607, 658, 898
TaiI ACGT 3 cut(s) 324, 379, 718
TaqI TCGA 6 cut(s) 318, 396, 515, 538, 910, 936
TaqII GACCGA 2 cut(s) 542, 1145
TasI AATT 5 cut(s) 93, 253, 634, 1209, 1299
TatI WGTACW 1 cut(s) 599
TfiI GAWTC 7 cut(s) 105, 294, 315, 958, 1054, 1100, 1144
Tru1I TTAA 7 cut(s) 407, 774, 782, 885, 906, 990, 1283
Tru9I TTAA 7 cut(s) 407, 774, 782, 885, 906, 990, 1283
TscAI CASTG 2 cut(s) 1188, 1209
TseFI GTSAC 1 cut(s) 726
TseI GCWGC 2 cut(s) 162, 1191
Tsp45I GTSAC 1 cut(s) 726
TspDTI ATGAA 4 cut(s) 360, 378, 931, 1364
TspRI CASTG 2 cut(s) 1188, 1209
Vha464I CTTAAG 1 cut(s) 884
VneI GTGCAC 1 cut(s) 764
VpaK11BI GGWCC 5 cut(s) 240, 675, 924, 1157, 1225
XagI CCTNNNNNAGG 1 cut(s) 682
XapI RAATTY 3 cut(s) 253, 634, 1209
XbaI TCTAGA 1 cut(s) 52
XceI RCATGY 2 cut(s) 1168, 1337
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 538
XmnI GAANNNNTTC 2 cut(s) 36, 570
XspI CTAG 3 cut(s) 53, 455, 950
ZraI GACGTC 2 cut(s) 322, 377
Zsp2I ATGCAT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.