pycom12g21890

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0020833
Physical Location & Seq
Forward (+)
22995732 .. 22999710
3979 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1869 bp
ATGGAGCAATATGAAGTTCTTGAGCAAATCGGTAAAGGGGCTTTCGGTTCTGCGCTTCTTGTCAGGCATAAGCATGAAAAGAAGAAGTACGTGTTGAAAAAGATTCGTCTTGCTCGCCAGACTGATAGATCGCGGATCGATCTGCACATCAGGAGGGAGCTCATTTCCAAGTTCAAAAATCCATTTATTGTGGAGTACAAAGATTCTTGGGTGGAAAAGGGTTGCTATGTGTGCATTATCATCGGTTATTGCGCAGGAGGGGACATGGCTGAAGCTATAAAAAAGGCCAATGGTCTTCTTTTCCCCGAAGAGTGTTCTAATATATTCTTGACAAAAGATCAAGACATACGCCTTGGTGACTTTGGACTTGCCAAAATGTTGACTTCAGATGATCTTGCCTCTTCGGTTGTTGGAACTCCTAGCTATATGTGTCCTGAGCTTCTTGCTGATATACCTTATGGTTCAAAATCAGATATCTGGTCTTTGGGATGCTGTATCTATGAAATGACTTCTCATAGGGCTGCATTTAAAGCGTTTGATATACAAGCACTGATTAACAAAATTAACAGGTCCATAGTGGCTCCCCTTCCCACAAAGTACACTGGTGCATTCCGAAGCCTTATTAAAAGCATGCTTCGGAAAAACCCAGAACTCAGGCCAAGTGCTGCTGAGCTGCTTGCTCACCCAGTCCTTCAGCCTCATGTTCACAAGGTTCATCTCAAACTCAATAACCCCAGACATGGAACTTTAGCGGTCAACTGGCCTGATACTGGTTACTTGAAGAAGACAAGGTTTTCAGAGCCAGAAGCAGTTCCTTTCTCCACTCGTCGTTACCAGGACAAGCGGCGTACATTCAGCAATGATAGGACTTTAAATCCTAGTATATCTGGAGGTGAGCAAGATTCTGTAAGCTCTACCCAAGAAATGCATGGCAGGCCAAAATATCTGAATCGAGAATTGGCAGGACATTCTGTTGGTGGCACTGGCGCCTATAGAAAAGCAGCCATACGCAAGCAGCCAATTGCTTCCAAAACTTCAAATATCATTAACAGTCGAGTTACTCCAGCAAAAGCTTCTGCCACTCCCAAGAGATTTATGGAGCCAAAGAATCGTGCATCGCTTTCTGTTTCACGCGCTCCGCAGAAGACAGTGAGTACAACTCGCAGAGCATCATTGCCATTAACAAGAGCTGCAACTCAACGGTCCCCTGTCTGGCCCAATGTTGGTGTTCTTCACTGTATTAAATCTCCCGACGTTTCTGTCAATTCCCCTCGAATTGACAGGATAGCTGAATTCCCATTAGCCTCGTATGAAGAACAATTATACGAAGAACAGTTTCTGCCTATCCATAGAAACTCATCGCCCTCAGTGCAAGGTTCGTCTGGTTCCCCCCACTCTGGTGACCGTTCAACGATGAAGGACAAATGCACCGTTCAGATAAGCGACAGAGCCTCTCGCAAACTTAGTTTCACAGGTGCATGGAAGGGAATTGGACCTGGCATGTTCAAAGCTGCACCAGACAATCAAAGCGATGAATGTTCGGATCAAAACGCAACTGCTGGAGCATCAAGCCGGACCTCATCAAACACGCGCCGCCAGCATTTTGACACCTCATCATTCCAGCAAAGGGCCGAAGCTTTAGAAGGGCTGCTCGAATTCAGTGCAAGATTGCTCCAGAACGAACGATATGACGAGCTTGGGGTGTTGCTGAAGCCTTTTGGGCCAGGGAAGGTCTCTCCTAGGGAAACTGCAATTTGGTTGACTAAAAGCATCAAGCAAAATAATGTCGACCAATGTTCGTACTCATCTGATCGATCAATTACAATGAGCGTTTGCAACATGTATTATATTTATTTCCAACTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

623

Amino Acids

69.51

Weight (kDa)

9.6

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 96 2.8e-11 Protein kinase domain
Pkinase PF00069 106 - 230 1.2e-28 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 106 - 226 4.2e-15 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020833)

Species Orthologous Gene IDs
malus_domestica MD04G1222000.v1.1 MD12G1238200.v1.1
prunus_persica Prupe.6G341100_v2.0.a1 Prupe.6G341100_v2.0.a1
pyrus_communis pycom12g21890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1259
Acc16I TGCGCA 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 986
AccI GTMKAC 1 cut(s) 1788
AccII CGCG 3 cut(s) 133, 1134, 1591
AciI CCGC 5 cut(s) 133, 752, 844, 1139, 1594
AclWI GGATC 2 cut(s) 143, 1551
AcsI RAATTY 2 cut(s) 1292, 1655
AcuI CTGAAG 4 cut(s) 291, 369, 677, 1730
AcyI GRCGYC 1 cut(s) 987
AfaI GTAC 6 cut(s) 89, 197, 599, 850, 1156, 1802
AfiI CCNNNNNNNGG 6 cut(s) 740, 770, 1212, 1223, 1397, 1627
AflIII ACRYGT 2 cut(s) 90, 1839
AgsI TTSAA 7 cut(s) 97, 175, 465, 781, 1038, 1410, 1507
AjnI CCWGG 3 cut(s) 834, 1495, 1723
AjuI GAANNNNNNNTTGG 2 cut(s) 941, 973
AleI CACNNNNGTG 1 cut(s) 1398
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 1738
AlwI GGATC 2 cut(s) 143, 1551
AlwNI CAGNNNCTG 1 cut(s) 1339
AoxI GGCC 7 cut(s) 285, 656, 761, 935, 1214, 1629, 1721
ApeKI GCWGC 8 cut(s) 521, 665, 673, 1001, 1015, 1190, 1511, 1648
ApoI RAATTY 2 cut(s) 1292, 1655
Asp700I GAANNNNTTC 2 cut(s) 810, 1335
AspA2I CCTAGG 1 cut(s) 1739
AspLEI GCGC 5 cut(s) 55, 254, 989, 1136, 1593
AspS9I GGNCC 7 cut(s) 570, 1203, 1215, 1493, 1575, 1629, 1721
AsuHPI GGTGA 4 cut(s) 368, 674, 905, 1412
AvaII GGWCC 4 cut(s) 570, 1203, 1493, 1575
AvrII CCTAGG 1 cut(s) 1739
BanI GGYRCC 1 cut(s) 986
BanII GRGCYC 1 cut(s) 162
BbsI GAAGAC 3 cut(s) 287, 791, 1151
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 8 cut(s) 508, 652, 660, 1013, 1027, 1177, 1498, 1635
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 1643, 1677
BciT130I CCWGG 3 cut(s) 836, 1497, 1725
BcoDI GTCTC 1 cut(s) 1738
BfaI CTAG 3 cut(s) 420, 879, 1740
BfmI CTRYAG 1 cut(s) 991
BfoI RGCGCY 1 cut(s) 990
BglI GCCNNNNNGGC 1 cut(s) 1720
BlnI CCTAGG 1 cut(s) 1739
BlpI GCTNAGC 1 cut(s) 669
Bme1390I CCNGG 3 cut(s) 836, 1497, 1725
Bme18I GGWCC 4 cut(s) 570, 1203, 1493, 1575
BmgT120I GGNCC 7 cut(s) 570, 1203, 1215, 1493, 1575, 1629, 1721
BmiI GGNNCC 5 cut(s) 582, 988, 1101, 1205, 1387
BmrFI CCNGG 3 cut(s) 836, 1497, 1725
BmrI ACTGGG 1 cut(s) 680
BmsI GCATC 5 cut(s) 479, 1124, 1178, 1574, 1779
BmuI ACTGGG 1 cut(s) 680
BpiI GAAGAC 3 cut(s) 287, 791, 1151
BplI GAGNNNNNCTC 2 cut(s) 1144, 1176
BpmI CTGGAG 4 cut(s) 909, 1047, 1581, 1658
Bpu10I CCTNAGC 1 cut(s) 435
Bpu1102I GCTNAGC 1 cut(s) 669
BpuEI CTTGAG 1 cut(s) 41
Bsa29I ATCGAT 2 cut(s) 138, 1813
BsaAI YACGTR 1 cut(s) 91
BsaHI GRCGYC 1 cut(s) 987
BsaI GGTCTC 1 cut(s) 1738
BsaJI CCNNGG 3 cut(s) 352, 1724, 1739
Bsc4I CCNNNNNNNGG 6 cut(s) 740, 770, 1212, 1223, 1397, 1627
Bse1I ACTGG 5 cut(s) 607, 686, 764, 775, 988
Bse3DI GCAATG 2 cut(s) 865, 1172
BseBI CCWGG 3 cut(s) 836, 1497, 1725
BseCI ATCGAT 2 cut(s) 138, 1813
BseDI CCNNGG 3 cut(s) 352, 1724, 1739
BseGI GGATG 1 cut(s) 494
BseLI CCNNNNNNNGG 6 cut(s) 740, 770, 1212, 1223, 1397, 1627
BseMI GCAATG 2 cut(s) 865, 1172
BseMII CTCAG 4 cut(s) 426, 660, 667, 1380
BseNI ACTGG 5 cut(s) 607, 686, 764, 775, 988
BseXI GCAGC 8 cut(s) 508, 652, 660, 1013, 1027, 1177, 1498, 1635
BsgI GTGCAG 2 cut(s) 128, 1497
Bsh1236I CGCG 3 cut(s) 133, 1134, 1591
BshFI GGCC 7 cut(s) 287, 658, 763, 937, 1216, 1631, 1723
BshNI GGYRCC 1 cut(s) 986
BshVI ATCGAT 2 cut(s) 138, 1813
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 1573
BslFI GGGAC 2 cut(s) 275, 1189
BslI CCNNNNNNNGG 6 cut(s) 740, 770, 1212, 1223, 1397, 1627
BsmAI GTCTC 1 cut(s) 1738
BsmFI GGGAC 2 cut(s) 275, 1189
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 7 cut(s) 287, 658, 763, 937, 1216, 1631, 1723
Bso31I GGTCTC 1 cut(s) 1738
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 8 cut(s) 128, 135, 139, 337, 391, 1543, 1810, 1814
Bsp1720I GCTNAGC 1 cut(s) 669
BspACI CCGC 5 cut(s) 133, 752, 844, 1139, 1594
BspANI GGCC 7 cut(s) 287, 658, 763, 937, 1216, 1631, 1723
BspCNI CTCAG 4 cut(s) 427, 661, 666, 1379
BspDI ATCGAT 2 cut(s) 138, 1813
BspFNI CGCG 3 cut(s) 133, 1134, 1591
BspLI GGNNCC 5 cut(s) 582, 988, 1101, 1205, 1387
BspPI GGATC 2 cut(s) 143, 1551
BspT107I GGYRCC 1 cut(s) 986
BspTNI GGTCTC 1 cut(s) 1738
BsrDI GCAATG 2 cut(s) 865, 1172
BsrI ACTGG 5 cut(s) 607, 686, 764, 775, 988
BssECI CCNNGG 3 cut(s) 352, 1724, 1739
BssMI GATC 8 cut(s) 128, 135, 139, 337, 391, 1543, 1810, 1814
BssNI GRCGYC 1 cut(s) 987
BssT1I CCWWGG 2 cut(s) 352, 1739
Bst2UI CCWGG 3 cut(s) 836, 1497, 1725
Bst4CI ACNGT 7 cut(s) 1052, 1150, 1203, 1238, 1335, 1406, 1432
Bst6I CTCTTC 2 cut(s) 303, 406
BstACI GRCGYC 1 cut(s) 987
BstBAI YACGTR 1 cut(s) 91
BstC8I GCNNGC 6 cut(s) 115, 632, 678, 935, 1013, 1598
BstDEI CTNAG 5 cut(s) 435, 653, 669, 1366, 1463
BstEII GGTNACC 1 cut(s) 1400
BstF5I GGATG 1 cut(s) 494
BstFNI CGCG 3 cut(s) 133, 1134, 1591
BstH2I RGCGCY 1 cut(s) 990
BstHHI GCGC 5 cut(s) 55, 254, 989, 1136, 1593
BstKTI GATC 8 cut(s) 131, 138, 142, 340, 394, 1546, 1813, 1817
BstMAI GTCTC 1 cut(s) 1738
BstMBI GATC 8 cut(s) 128, 135, 139, 337, 391, 1543, 1810, 1814
BstMWI GCNNNNNNNGC 6 cut(s) 231, 530, 934, 1369, 1597, 1720
BstNI CCWGG 3 cut(s) 836, 1497, 1725
BstNSI RCATGY 3 cut(s) 634, 1504, 1843
BstPI GGTNACC 1 cut(s) 1400
BstSCI CCNGG 3 cut(s) 834, 1495, 1723
BstSFI CTRYAG 1 cut(s) 991
BstUI CGCG 3 cut(s) 133, 1134, 1591
BstV1I GCAGC 8 cut(s) 508, 652, 660, 1013, 1027, 1177, 1498, 1635
BstV2I GAAGAC 3 cut(s) 287, 791, 1151
Bsu15I ATCGAT 2 cut(s) 138, 1813
BsuRI GGCC 7 cut(s) 287, 658, 763, 937, 1216, 1631, 1723
BsuTUI ATCGAT 2 cut(s) 138, 1813
BtgZI GCGATG 3 cut(s) 1101, 1344, 1545
BtsCI GGATG 1 cut(s) 494
BtsIMutI CAGTG 7 cut(s) 548, 600, 981, 1155, 1234, 1374, 1666
Cac8I GCNNGC 6 cut(s) 115, 632, 678, 935, 1013, 1598
CaiI CAGNNNCTG 1 cut(s) 1339
CfoI GCGC 5 cut(s) 55, 254, 989, 1136, 1593
Cfr13I GGNCC 7 cut(s) 570, 1203, 1215, 1493, 1575, 1629, 1721
ClaI ATCGAT 2 cut(s) 138, 1813
Csp6I GTAC 6 cut(s) 88, 196, 598, 849, 1155, 1801
CviAII CATG 9 cut(s) 74, 265, 631, 701, 740, 929, 1479, 1501, 1840
CviQI GTAC 6 cut(s) 88, 196, 598, 849, 1155, 1801
DdeI CTNAG 5 cut(s) 435, 653, 669, 1366, 1463
DinI GGCGCC 1 cut(s) 988
DpnI GATC 8 cut(s) 130, 137, 141, 339, 393, 1545, 1812, 1816
DpnII GATC 8 cut(s) 128, 135, 139, 337, 391, 1543, 1810, 1814
DraI TTTAAA 2 cut(s) 529, 873
DrdI GACNNNNNNGTC 1 cut(s) 1259
DseDI GACNNNNNNGTC 1 cut(s) 1259
Eam1104I CTCTTC 2 cut(s) 303, 406
EarI CTCTTC 2 cut(s) 303, 406
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 2 cut(s) 352, 1739
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 1738
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 4 cut(s) 570, 1203, 1493, 1575
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 4 cut(s) 291, 369, 677, 1730
Eco91I GGTNACC 1 cut(s) 1400
EcoICRI GAGCTC 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 1400
EcoRI GAATTC 2 cut(s) 1292, 1655
EcoRII CCWGG 3 cut(s) 834, 1495, 1723
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 2 cut(s) 352, 1739
EcoT22I ATGCAT 1 cut(s) 930
EcoT38I GRGCYC 1 cut(s) 162
EgeI GGCGCC 1 cut(s) 988
EheI GGCGCC 1 cut(s) 988
ErhI CCWWGG 2 cut(s) 352, 1739
FaeI CATG 9 cut(s) 77, 268, 634, 704, 743, 932, 1482, 1504, 1843
FaqI GGGAC 2 cut(s) 275, 1189
FatI CATG 9 cut(s) 73, 264, 630, 700, 739, 928, 1478, 1500, 1839
FblI GTMKAC 1 cut(s) 1788
FokI GGATG 1 cut(s) 501
FriOI GRGCYC 1 cut(s) 162
FspBI CTAG 3 cut(s) 420, 879, 1740
FspI TGCGCA 1 cut(s) 253
GlaI GCGC 5 cut(s) 54, 253, 988, 1135, 1592
GsuI CTGGAG 4 cut(s) 909, 1047, 1581, 1658
HaeII RGCGCY 1 cut(s) 990
HaeIII GGCC 7 cut(s) 287, 658, 763, 937, 1216, 1631, 1723
HapII CCGG 1 cut(s) 1573
HhaI GCGC 5 cut(s) 55, 254, 989, 1136, 1593
Hin1I GRCGYC 1 cut(s) 987
Hin1II CATG 9 cut(s) 77, 268, 634, 704, 743, 932, 1482, 1504, 1843
Hin6I GCGC 5 cut(s) 53, 252, 987, 1134, 1591
HinP1I GCGC 5 cut(s) 53, 252, 987, 1134, 1591
HincII GTYRAC 4 cut(s) 381, 757, 1761, 1789
HindII GTYRAC 4 cut(s) 381, 757, 1761, 1789
HindIII AAGCTT 2 cut(s) 1071, 1635
HinfI GANTC 5 cut(s) 103, 203, 902, 949, 1108
HpaII CCGG 1 cut(s) 1573
HphI GGTGA 4 cut(s) 368, 674, 905, 1412
Hpy166II GTNNAC 6 cut(s) 381, 600, 706, 757, 1761, 1789
Hpy188I TCNGA 9 cut(s) 388, 472, 614, 639, 799, 948, 1437, 1543, 1810
Hpy188III TCNNGA 9 cut(s) 20, 151, 328, 341, 434, 888, 953, 1250, 1675
Hpy8I GTNNAC 6 cut(s) 381, 600, 706, 757, 1761, 1789
Hpy99I CGWCG 2 cut(s) 831, 1256
HpyAV CCTTC 6 cut(s) 596, 701, 1411, 1477, 1637, 1723
HpyCH4III ACNGT 7 cut(s) 1052, 1150, 1203, 1238, 1335, 1406, 1432
HpyCH4IV ACGT 2 cut(s) 90, 1254
HpyF10VI GCNNNNNNNGC 6 cut(s) 231, 530, 934, 1369, 1597, 1720
HpyF3I CTNAG 5 cut(s) 435, 653, 669, 1366, 1463
HpySE526I ACGT 2 cut(s) 90, 1254
Hsp92I GRCGYC 1 cut(s) 987
Hsp92II CATG 9 cut(s) 77, 268, 634, 704, 743, 932, 1482, 1504, 1843
HspAI GCGC 5 cut(s) 53, 252, 987, 1134, 1591
KasI GGCGCC 1 cut(s) 986
Kzo9I GATC 8 cut(s) 128, 135, 139, 337, 391, 1543, 1810, 1814
LmnI GCTCC 7 cut(s) 4, 157, 586, 1099, 1141, 1562, 1677
Lsp1109I GCAGC 8 cut(s) 508, 652, 660, 1013, 1027, 1177, 1498, 1635
LweI GCATC 5 cut(s) 479, 1124, 1178, 1574, 1779
MaeI CTAG 3 cut(s) 420, 879, 1740
MaeII ACGT 2 cut(s) 90, 1254
MaeIII GTNAC 5 cut(s) 356, 773, 830, 1057, 1400
MalI GATC 8 cut(s) 130, 137, 141, 339, 393, 1545, 1812, 1816
MboI GATC 8 cut(s) 128, 135, 139, 337, 391, 1543, 1810, 1814
MfeI CAATTG 1 cut(s) 1020
MhlI GDGCHC 1 cut(s) 162
Mly113I GGCGCC 1 cut(s) 987
MmeI TCCRAC 1 cut(s) 391
Mph1103I ATGCAT 1 cut(s) 930
MroXI GAANNNNTTC 2 cut(s) 810, 1335
MseI TTAA 8 cut(s) 528, 555, 564, 624, 872, 1047, 1181, 1242
MslI CAYNNNNRTG 2 cut(s) 72, 1398
MspI CCGG 1 cut(s) 1573
MspR9I CCNGG 3 cut(s) 836, 1497, 1725
MunI CAATTG 1 cut(s) 1020
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 3 cut(s) 836, 1497, 1725
MvnI CGCG 3 cut(s) 133, 1134, 1591
MwoI GCNNNNNNNGC 6 cut(s) 231, 530, 934, 1369, 1597, 1720
NarI GGCGCC 1 cut(s) 987
NdeII GATC 8 cut(s) 128, 135, 139, 337, 391, 1543, 1810, 1814
NlaIII CATG 9 cut(s) 77, 268, 634, 704, 743, 932, 1482, 1504, 1843
NlaIV GGNNCC 5 cut(s) 582, 988, 1101, 1205, 1387
NmuCI GTSAC 2 cut(s) 356, 1400
NsbI TGCGCA 1 cut(s) 253
NsiI ATGCAT 1 cut(s) 930
NspI RCATGY 3 cut(s) 634, 1504, 1843
OliI CACNNNNGTG 1 cut(s) 1398
PaeI GCATGC 1 cut(s) 634
PciI ACATGT 1 cut(s) 1839
PctI GAATGC 1 cut(s) 608
PdmI GAANNNNTTC 2 cut(s) 810, 1335
PfeI GAWTC 5 cut(s) 103, 203, 902, 949, 1108
PluTI GGCGCC 1 cut(s) 990
Ppu21I YACGTR 1 cut(s) 91
PscI ACATGT 1 cut(s) 1839
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 3 cut(s) 834, 1495, 1723
PspEI GGTNACC 1 cut(s) 1400
PspGI CCWGG 3 cut(s) 834, 1495, 1723
PspN4I GGNNCC 5 cut(s) 582, 988, 1101, 1205, 1387
PspPI GGNCC 7 cut(s) 570, 1203, 1215, 1493, 1575, 1629, 1721
PstNI CAGNNNCTG 1 cut(s) 1339
RsaI GTAC 6 cut(s) 89, 197, 599, 850, 1156, 1802
RsaNI GTAC 6 cut(s) 88, 196, 598, 849, 1155, 1801
RseI CAYNNNNRTG 2 cut(s) 72, 1398
SacI GAGCTC 1 cut(s) 162
SalI GTCGAC 1 cut(s) 1787
SaqAI TTAA 8 cut(s) 528, 555, 564, 624, 872, 1047, 1181, 1242
Sau3AI GATC 8 cut(s) 128, 135, 139, 337, 391, 1543, 1810, 1814
Sau96I GGNCC 7 cut(s) 570, 1203, 1215, 1493, 1575, 1629, 1721
ScrFI CCNGG 3 cut(s) 836, 1497, 1725
SduI GDGCHC 1 cut(s) 162
SfaNI GCATC 5 cut(s) 479, 1124, 1178, 1574, 1779
SfcI CTRYAG 1 cut(s) 991
SfoI GGCGCC 1 cut(s) 988
SinI GGWCC 4 cut(s) 570, 1203, 1493, 1575
SmiMI CAYNNNNRTG 2 cut(s) 72, 1398
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SphI GCATGC 1 cut(s) 634
SsiI CCGC 5 cut(s) 133, 752, 844, 1139, 1594
SspDI GGCGCC 1 cut(s) 986
SspMI CTAG 3 cut(s) 420, 879, 1740
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 834, 1495, 1723
StyI CCWWGG 2 cut(s) 352, 1739
TaaI ACNGT 7 cut(s) 1052, 1150, 1203, 1238, 1335, 1406, 1432
TaiI ACGT 2 cut(s) 93, 1257
TaqI TCGA 8 cut(s) 138, 952, 1054, 1273, 1653, 1788, 1813, 1863
TatI WGTACW 3 cut(s) 195, 597, 1154
TauI GCSGC 2 cut(s) 847, 1596
TfiI GAWTC 5 cut(s) 103, 203, 902, 949, 1108
Tru1I TTAA 8 cut(s) 528, 555, 564, 624, 872, 1047, 1181, 1242
Tru9I TTAA 8 cut(s) 528, 555, 564, 624, 872, 1047, 1181, 1242
TscAI CASTG 7 cut(s) 555, 607, 988, 1155, 1241, 1374, 1666
TseFI GTSAC 2 cut(s) 356, 1400
TseI GCWGC 8 cut(s) 521, 665, 673, 1001, 1015, 1190, 1511, 1648
Tsp45I GTSAC 2 cut(s) 356, 1400
TspDTI ATGAA 7 cut(s) 27, 90, 516, 704, 1326, 1430, 1548
TspRI CASTG 7 cut(s) 555, 607, 988, 1155, 1241, 1374, 1666
VpaK11BI GGWCC 4 cut(s) 570, 1203, 1493, 1575
XapI RAATTY 2 cut(s) 1292, 1655
XceI RCATGY 3 cut(s) 634, 1504, 1843
XcmI CCANNNNNNNNNTGG 2 cut(s) 926, 1093
XmaJI CCTAGG 1 cut(s) 1739
XmiI GTMKAC 1 cut(s) 1788
XmnI GAANNNNTTC 2 cut(s) 810, 1335
XspI CTAG 3 cut(s) 420, 879, 1740
Zsp2I ATGCAT 1 cut(s) 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.