pycom13g03620

RuBisCO catalyzes two reactions the carboxylation of D- ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0024250
Physical Location & Seq
Reverse (-)
2398770 .. 2399776
1007 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 333 bp
ATGGCTTCCCCTAAGGTTTCTTCAGCCGCTGTTACCTCCGTCCACCGCACCAGGTCCCCAGCTCAGGCAAGCATGGTGGCACCCTTCAACGGGCTCAAGTCCTCCTCAGCCTTCCCTGTGACCCGCAAGGCCAACGATCTTACCTCCATTGCCAGCAACGGTGGAAGAGTCCAGTCTTCCCTCTTCGGATGCACTGATTCTTCCCAAGTTCTAAAGGAGTTCGAGGAGGCCAAGACCACCTACCCCGAATCCTTCGTCCGGATCATCGGATTCGACAAGATCCGCCAAACACAGTGCGTCAGTTTCATCGCTTACAAGCCCCCAGGCTCCTAA

Protein Analysis

111

Amino Acids

11.75

Weight (kDa)

9.76

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RbcS PF12338 2 - 45 7.2e-20 Ribulose-1,5-bisphosphate carboxylase small subunit
RuBisCO_small PF00101 60 - 107 3.8e-14 Ribulose bisphosphate carboxylase, small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0024250)

Species Orthologous Gene IDs
malus_domestica MD13G1041800.v1.1
pyrus_communis pycom13g03620
rosa_multiflora Rmu_co8397811.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 79
AccIII TCCGGA 1 cut(s) 258
AciI CCGC 4 cut(s) 27, 46, 124, 283
AclWI GGATC 2 cut(s) 269, 274
AcuI CTGAAG 1 cut(s) 6
AfiI CCNNNNNNNGG 4 cut(s) 64, 89, 90, 258
AgsI TTSAA 1 cut(s) 88
AjnI CCWGG 2 cut(s) 50, 322
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
AlwI GGATC 2 cut(s) 269, 274
AlwNI CAGNNNCTG 1 cut(s) 29
Aor13HI TCCGGA 1 cut(s) 258
AoxI GGCC 2 cut(s) 129, 228
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AspS9I GGNCC 1 cut(s) 54
AvaII GGWCC 1 cut(s) 54
AxyI CCTNAGG 1 cut(s) 12
BanI GGYRCC 1 cut(s) 79
BanII GRGCYC 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 168
BbvCI CCTCAGC 1 cut(s) 106
BciT130I CCWGG 2 cut(s) 52, 324
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 2 cut(s) 52, 324
Bme18I GGWCC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 3 cut(s) 56, 81, 328
BmrFI CCNGG 2 cut(s) 52, 324
BmsI GCATC 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 168
Bpu10I CCTNAGC 2 cut(s) 63, 106
BpuEI CTTGAG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 322
BsaWI WCCGGW 1 cut(s) 258
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 89, 90, 258
Bse1I ACTGG 1 cut(s) 172
Bse21I CCTNAGG 1 cut(s) 12
Bse3DI GCAATG 1 cut(s) 147
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 2 cut(s) 52, 324
BseDI CCNNGG 1 cut(s) 322
BseGI GGATG 1 cut(s) 194
BseLI CCNNNNNNNGG 4 cut(s) 64, 89, 90, 258
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 2 cut(s) 77, 120
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 2 cut(s) 94, 239
BseYI CCCAGC 1 cut(s) 58
BshFI GGCC 2 cut(s) 131, 230
BshNI GGYRCC 1 cut(s) 79
BsiSI CCGG 1 cut(s) 259
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 4 cut(s) 64, 89, 90, 258
BsmFI GGGAC 1 cut(s) 40
BsnI GGCC 2 cut(s) 131, 230
Bsp1286I GDGCHC 1 cut(s) 96
Bsp13I TCCGGA 1 cut(s) 258
Bsp143I GATC 3 cut(s) 136, 261, 279
BspACI CCGC 4 cut(s) 27, 46, 124, 283
BspANI GGCC 2 cut(s) 131, 230
BspCNI CTCAG 2 cut(s) 76, 119
BspEI TCCGGA 1 cut(s) 258
BspLI GGNNCC 3 cut(s) 56, 81, 328
BspPI GGATC 2 cut(s) 269, 274
BspT107I GGYRCC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 147
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 1 cut(s) 322
BssMI GATC 3 cut(s) 136, 261, 279
Bst2UI CCWGG 2 cut(s) 52, 324
Bst4CI ACNGT 2 cut(s) 161, 294
Bst6I CTCTTC 2 cut(s) 160, 188
BstC8I GCNNGC 2 cut(s) 70, 154
BstDEI CTNAG 3 cut(s) 12, 63, 106
BstF5I GGATG 1 cut(s) 194
BstKTI GATC 3 cut(s) 139, 264, 282
BstMBI GATC 3 cut(s) 136, 261, 279
BstNI CCWGG 2 cut(s) 52, 324
BstSCI CCNGG 2 cut(s) 50, 322
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
Bsu36I CCTNAGG 1 cut(s) 12
BsuRI GGCC 2 cut(s) 131, 230
BtgZI GCGATG 1 cut(s) 292
BtsCI GGATG 1 cut(s) 194
BtsIMutI CAGTG 2 cut(s) 192, 299
Cac8I GCNNGC 2 cut(s) 70, 154
CaiI CAGNNNCTG 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 54
CseI GACGC 1 cut(s) 286
CsiI ACCWGGT 1 cut(s) 50
CspCI CAANNNNNGTGG 2 cut(s) 57, 92
CviAII CATG 1 cut(s) 73
CviJI RGCY 9 cut(s) 5, 26, 62, 94, 110, 131, 230, 319, 327
CviKI_1 RGCY 9 cut(s) 5, 26, 62, 94, 110, 131, 230, 319, 327
DdeI CTNAG 3 cut(s) 12, 63, 106
DpnI GATC 3 cut(s) 138, 263, 281
DpnII GATC 3 cut(s) 136, 261, 279
Eam1104I CTCTTC 2 cut(s) 160, 188
EarI CTCTTC 2 cut(s) 160, 188
EciI GGCGGA 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 6
Eco81I CCTNAGG 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 54
EcoRII CCWGG 2 cut(s) 50, 322
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 1 cut(s) 76
FaiI YATR 1 cut(s) 74
FaqI GGGAC 1 cut(s) 40
FatI CATG 1 cut(s) 72
FauI CCCGC 1 cut(s) 131
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 1 cut(s) 201
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 1 cut(s) 27
GluI GCNGC 1 cut(s) 27
GsaI CCCAGC 1 cut(s) 62
HaeIII GGCC 2 cut(s) 131, 230
HapII CCGG 1 cut(s) 259
HgaI GACGC 1 cut(s) 286
Hin1II CATG 1 cut(s) 76
HinfI GANTC 4 cut(s) 168, 197, 248, 270
HpaII CCGG 1 cut(s) 259
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 2 cut(s) 188, 269
Hpy188III TCNNGA 1 cut(s) 259
Hpy8I GTNNAC 1 cut(s) 43
HpyAV CCTTC 3 cut(s) 94, 121, 262
HpyCH4III ACNGT 2 cut(s) 161, 294
HpyCH4V TGCA 1 cut(s) 192
HpyF3I CTNAG 3 cut(s) 12, 63, 106
Hsp92II CATG 1 cut(s) 76
Kpn2I TCCGGA 1 cut(s) 258
Kzo9I GATC 3 cut(s) 136, 261, 279
LmnI GCTCC 1 cut(s) 332
LpnPI CCDG 9 cut(s) 37, 50, 64, 72, 129, 166, 185, 272, 309
LweI GCATC 1 cut(s) 179
MabI ACCWGGT 1 cut(s) 50
MaeIII GTNAC 2 cut(s) 31, 118
MalI GATC 3 cut(s) 138, 263, 281
MboI GATC 3 cut(s) 136, 261, 279
MboII GAAGA 5 cut(s) 12, 168, 175, 177, 192
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 96
MlyI GAGTC 1 cut(s) 177
MnlI CCTC 7 cut(s) 46, 112, 115, 154, 191, 217, 220
MroI TCCGGA 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 1 cut(s) 259
MspR9I CCNGG 2 cut(s) 52, 324
MvaI CCWGG 2 cut(s) 52, 324
NdeII GATC 3 cut(s) 136, 261, 279
NlaIII CATG 1 cut(s) 76
NlaIV GGNNCC 3 cut(s) 56, 81, 328
NmuCI GTSAC 1 cut(s) 118
PfeI GAWTC 3 cut(s) 197, 248, 270
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
PpuMI RGGWCCY 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 2 cut(s) 50, 322
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 2 cut(s) 50, 322
PspN4I GGNNCC 3 cut(s) 56, 81, 328
PspPI GGNCC 1 cut(s) 54
PspPPI RGGWCCY 1 cut(s) 54
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 279
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 3 cut(s) 136, 261, 279
Sau96I GGNCC 1 cut(s) 54
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 2 cut(s) 52, 324
SduI GDGCHC 1 cut(s) 96
SetI ASST 6 cut(s) 18, 38, 56, 64, 146, 242
SexAI ACCWGGT 1 cut(s) 50
SfaNI GCATC 1 cut(s) 179
SinI GGWCC 1 cut(s) 54
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SsiI CCGC 4 cut(s) 27, 46, 124, 283
StyD4I CCNGG 2 cut(s) 50, 322
TaaI ACNGT 2 cut(s) 161, 294
TaqI TCGA 2 cut(s) 222, 273
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 3 cut(s) 197, 248, 270
TscAI CASTG 2 cut(s) 199, 299
TseFI GTSAC 1 cut(s) 118
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 1 cut(s) 295
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 2 cut(s) 199, 299
VpaK11BI GGWCC 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.