pycom13g20820

Retroviral aspartyl protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0029400
Physical Location & Seq
Reverse (-)
16981885 .. 16982466
582 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g20820.1

Sequence Viewer

Length: 582 bp
ATGCCCCGTCACGCTCCGACGACTCGTTTAACAACCATGGATGCACGTCTCATTGCCGTTGAAGCTACCTTGGCGGACCTCCCCAACCTCCTTCGAAACTCCATCGCCGCCGCGAATGCCGACCTCTCAGCCGTGATATCTACTGACTTCGATGCTAAGTTAGCTTTCTCCTTCGAGCAATTCCGGCGTGAACAATCCTTTGTCGGTGGTGTCGGATCTTCCAATCTAGCTTCCTTTGCCGCTTATCTTATCTCACCTCCAGTTCCAGTCACGGATGATATTGATTTTCGTCTTTTGGAGACTCCTCGACACCCTCGTCGTGATCAATATGAGCCAGGGGCGGGCTGCCTAAACTTCCGTGTCAGCAACGGGTGGATTTTCCCCGATTTTCCGATGGTGATGATCCTATCACTTGGATCTACAATGCTAAGCAATATTTTTCTTGTTACAACACTCCAGATCCACAAAAGGTCTTTACAGCCTCCTTTTACTTGGAAAATGAGGCGATGCAGTGGTTCAAATGGCGCAATTGCTTACTCACTACTCCTACTTGGGAGGAATTTACCAGTGCACTTTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag

Protein Analysis

194

Amino Acids

21.38

Weight (kDa)

9.18

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029400)

Species Orthologous Gene IDs
pyrus_communis pycom11g08520 pycom13g20820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 315
AccII CGCG 1 cut(s) 113
AciI CCGC 5 cut(s) 74, 108, 111, 240, 341
AclWI GGATC 4 cut(s) 223, 397, 424, 454
AcsI RAATTY 1 cut(s) 559
AfiI CCNNNNNNNGG 1 cut(s) 341
AgsI TTSAA 2 cut(s) 62, 519
AjiI CACGTC 1 cut(s) 47
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 3 cut(s) 65, 164, 230
AluI AGCT 3 cut(s) 65, 164, 230
Alw21I GWGCWC 1 cut(s) 573
Alw26I GTCTC 2 cut(s) 53, 293
Alw44I GTGCAC 1 cut(s) 569
AlwI GGATC 4 cut(s) 223, 397, 424, 454
ApaLI GTGCAC 1 cut(s) 569
ApeKI GCWGC 1 cut(s) 345
ApoI RAATTY 1 cut(s) 559
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 246, 409
AsuII TTCGAA 1 cut(s) 94
AvaII GGWCC 1 cut(s) 76
BaeGI GKGCMC 1 cut(s) 573
Bbv12I GWGCWC 1 cut(s) 573
BbvI GCAGC 1 cut(s) 332
BccI CCATC 2 cut(s) 110, 388
BceAI ACGGC 2 cut(s) 41, 116
BciT130I CCWGG 1 cut(s) 336
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 53, 293
BfaI CTAG 1 cut(s) 227
BisI GCNGC 4 cut(s) 108, 111, 240, 346
BlpI GCTNAGC 1 cut(s) 428
BlsI GCNGC 4 cut(s) 109, 112, 241, 347
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 76
BmgBI CACGTC 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 76
BmrFI CCNGG 1 cut(s) 336
BmsI GCATC 3 cut(s) 31, 142, 497
BpmI CTGGAG 2 cut(s) 243, 440
Bpu1102I GCTNAGC 1 cut(s) 428
Bpu14I TTCGAA 1 cut(s) 94
BsaJI CCNNGG 3 cut(s) 36, 69, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse1I ACTGG 3 cut(s) 260, 266, 566
Bse3DI GCAATG 1 cut(s) 51
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 3 cut(s) 36, 69, 335
BseGI GGATG 2 cut(s) 46, 280
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 3 cut(s) 260, 266, 566
BseRI GAGGAG 1 cut(s) 294
BseSI GKGCMC 1 cut(s) 573
BseXI GCAGC 1 cut(s) 332
Bsh1236I CGCG 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 573
BsiSI CCGG 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 341
BsmAI GTCTC 2 cut(s) 53, 293
BsmBI CGTCTC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 121
Bsp119I TTCGAA 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 573
Bsp143I GATC 5 cut(s) 215, 322, 402, 416, 459
Bsp1720I GCTNAGC 1 cut(s) 428
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 5 cut(s) 74, 108, 111, 240, 341
BspCNI CTCAG 1 cut(s) 140
BspFNI CGCG 1 cut(s) 113
BspPI GGATC 4 cut(s) 223, 397, 424, 454
BspT104I TTCGAA 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 3 cut(s) 260, 266, 566
BssECI CCNNGG 3 cut(s) 36, 69, 335
BssMI GATC 5 cut(s) 215, 322, 402, 416, 459
BssT1I CCWWGG 2 cut(s) 36, 69
Bst2UI CCWGG 1 cut(s) 336
BstBI TTCGAA 1 cut(s) 94
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 3 cut(s) 127, 156, 428
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 2 cut(s) 46, 280
BstFNI CGCG 1 cut(s) 113
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 5 cut(s) 218, 325, 405, 419, 462
BstMAI GTCTC 2 cut(s) 53, 293
BstMBI GATC 5 cut(s) 215, 322, 402, 416, 459
BstMWI GCNNNNNNNGC 6 cut(s) 62, 71, 116, 161, 184, 236
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 334
BstSLI GKGCMC 1 cut(s) 573
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 332
BstX2I RGATCY 3 cut(s) 215, 416, 459
BstYI RGATCY 3 cut(s) 215, 416, 459
BtgI CCRYGG 1 cut(s) 36
BtgZI GCGATG 2 cut(s) 88, 520
BtrI CACGTC 1 cut(s) 47
BtsCI GGATG 2 cut(s) 46, 280
BtsI GCAGTG 1 cut(s) 517
BtsIMutI CAGTG 2 cut(s) 517, 573
Cac8I GCNNGC 1 cut(s) 343
CfoI GCGC 1 cut(s) 527
Cfr13I GGNCC 1 cut(s) 76
CviAII CATG 1 cut(s) 37
CviJI RGCY 7 cut(s) 65, 131, 164, 230, 334, 345, 481
CviKI_1 RGCY 7 cut(s) 65, 131, 164, 230, 334, 345, 481
DdeI CTNAG 3 cut(s) 127, 156, 428
DpnI GATC 5 cut(s) 217, 324, 404, 418, 461
DpnII GATC 5 cut(s) 215, 322, 402, 416, 459
DrdI GACNNNNNNGTC 1 cut(s) 315
DseDI GACNNNNNNGTC 1 cut(s) 315
EciI GGCGGA 1 cut(s) 89
Eco130I CCWWGG 2 cut(s) 36, 69
Eco32I GATATC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 334
EcoRV GATATC 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 36, 69
ErhI CCWWGG 2 cut(s) 36, 69
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 1 cut(s) 40
FaiI YATR 3 cut(s) 38, 330, 580
FatI CATG 1 cut(s) 36
FauI CCCGC 1 cut(s) 334
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 4 cut(s) 108, 111, 240, 346
FokI GGATG 2 cut(s) 53, 287
Fsp4HI GCNGC 4 cut(s) 108, 111, 240, 346
FspBI CTAG 1 cut(s) 227
GlaI GCGC 1 cut(s) 526
GluI GCNGC 4 cut(s) 108, 111, 240, 346
GsuI CTGGAG 2 cut(s) 243, 440
HapII CCGG 1 cut(s) 184
HhaI GCGC 1 cut(s) 527
Hin1II CATG 1 cut(s) 40
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HinfI GANTC 2 cut(s) 22, 301
HpaII CCGG 1 cut(s) 184
HphI GGTGA 2 cut(s) 246, 409
Hpy166II GTNNAC 2 cut(s) 191, 571
Hpy188I TCNGA 3 cut(s) 18, 215, 393
Hpy188III TCNNGA 2 cut(s) 320, 457
Hpy8I GTNNAC 2 cut(s) 191, 571
Hpy99I CGWCG 2 cut(s) 22, 321
HpyAV CCTTC 2 cut(s) 101, 181
HpyCH4IV ACGT 1 cut(s) 46
HpyCH4V TGCA 3 cut(s) 44, 510, 571
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 71, 116, 161, 184, 236
HpyF3I CTNAG 3 cut(s) 127, 156, 428
HpySE526I ACGT 1 cut(s) 46
Hsp92II CATG 1 cut(s) 40
HspAI GCGC 1 cut(s) 525
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 5 cut(s) 215, 322, 402, 416, 459
LmnI GCTCC 1 cut(s) 19
LpnPI CCDG 6 cut(s) 197, 273, 279, 321, 348, 470
Lsp1109I GCAGC 1 cut(s) 332
LweI GCATC 3 cut(s) 31, 142, 497
MaeI CTAG 1 cut(s) 227
MaeII ACGT 1 cut(s) 46
MaeIII GTNAC 3 cut(s) 8, 268, 445
MalI GATC 5 cut(s) 217, 324, 404, 418, 461
MboI GATC 5 cut(s) 215, 322, 402, 416, 459
MboII GAAGA 1 cut(s) 210
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 3 cut(s) 215, 416, 459
MhlI GDGCHC 1 cut(s) 573
MluCI AATT 3 cut(s) 179, 528, 559
MlyI GAGTC 2 cut(s) 16, 295
MmeI TCCRAC 2 cut(s) 41, 193
MnlI CCTC 9 cut(s) 89, 98, 134, 267, 315, 324, 492, 495, 549
MseI TTAA 1 cut(s) 29
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 1 cut(s) 336
MunI CAATTG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 121
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 6 cut(s) 62, 71, 116, 161, 184, 236
NcoI CCATGG 1 cut(s) 36
NdeII GATC 5 cut(s) 215, 322, 402, 416, 459
NlaIII CATG 1 cut(s) 40
NmuCI GTSAC 2 cut(s) 8, 268
NspV TTCGAA 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 313
PctI GAATGC 1 cut(s) 121
PkrI GCNGC 4 cut(s) 109, 112, 241, 347
PleI GAGTC 2 cut(s) 16, 295
PpsI GAGTC 2 cut(s) 16, 295
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspPI GGNCC 1 cut(s) 76
PsuI RGATCY 3 cut(s) 215, 416, 459
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 4 cut(s) 108, 111, 240, 346
Sau3AI GATC 5 cut(s) 215, 322, 402, 416, 459
Sau96I GGNCC 1 cut(s) 76
SchI GAGTC 2 cut(s) 16, 295
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 1 cut(s) 573
SfaNI GCATC 3 cut(s) 31, 142, 497
SfuI TTCGAA 1 cut(s) 94
SinI GGWCC 1 cut(s) 76
Sse9I AATT 3 cut(s) 179, 528, 559
SsiI CCGC 5 cut(s) 74, 108, 111, 240, 341
SspI AATATT 1 cut(s) 436
SspMI CTAG 1 cut(s) 227
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 2 cut(s) 36, 69
TaiI ACGT 1 cut(s) 49
TaqI TCGA 4 cut(s) 94, 150, 174, 307
TasI AATT 3 cut(s) 179, 528, 559
TauI GCSGC 3 cut(s) 110, 113, 242
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 2 cut(s) 517, 573
TseFI GTSAC 2 cut(s) 8, 268
TseI GCWGC 1 cut(s) 345
Tsp45I GTSAC 2 cut(s) 8, 268
TspGWI ACGGA 2 cut(s) 287, 347
TspRI CASTG 2 cut(s) 517, 573
VneI GTGCAC 1 cut(s) 569
VpaK11BI GGWCC 1 cut(s) 76
XapI RAATTY 1 cut(s) 559
XspI CTAG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.