pycom13g24390

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0020428
Physical Location & Seq
Forward (+)
21551416 .. 21554066
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g24390.7

Sequence Viewer

Length: 1857 bp
ATGGGCATAATTCGTTATTCACATAACCCTGACTTGTCAAATATTCACTCAAATGGTTATACCACATCTGCCCGAATTGTTTCAATCCATAGAAACTATTTGAACCACTCAATGGAAGTCCTTCTGGAACTTTCATATAGATTTCCGTATCTAGATCTCCATAGAGATACGCAGTTACCACCAAAACGATATAATGTACATTACGGGGGAATATGTCTCCTCGCGTGCTTTGTATCGCACTATTTCATTCTTCTCATTACGCTTCCTCATGAAACCCCACTTGTAGCCAATAGGCTTCACATATGGTGGTATAGAAACTATAGGTCCAAACACTTTACGTTTCGCAACCATTCTACGTCGACATTCATCAATGAAACATGGTTCAATGTCATCACTAAGCATGATGTCAGTAGCTACTGTGTACGCAAATGCATCATCGACAATCATCTTATTCCGATTCCAAACCTCATCCAATATTGCGTAGTGGACCGAAATCTCGTGATTCTCGAGAGGTCAGGTTGCCTTGTCGAAGACATTACCTTGGTGCCGATTTCCTCTTCCGGGGTTGTGAATCCTTTGAACCAAAGGGTTTACCACTCTTCAGGGTCGGAGCAGATGATTGGCTACCTGCCGATGTATCGGGCTATGTGGAAAGTACAGCCTCCCCACTTTCAAGGCCTGTCTAGTCTTCCCAAGCAGGATTACAACGTACATGAGGTACGTCTATCCTTGCATGTGCGTTTTAAAAGCATTTGGCATACTTTGAGCAATGCTCTGAAGATCTATAATTCGTCACACTTCAGTTTTAGATTGGACAATCGTTCTCTAGGAACATTAGCATGCTTATCTCCCTCTAGCGACATGAAGACTCTCCTATCAAGGGTTCTAAATAGTGAACAATTGATGGCGAATCATAACCGACATAGATTCCTATTTTTCTCTAAGGACCCATTTTGGTACGTAGTGGCGGCACTATTGGCACATAAACTGCACACCCAAACACGTGTCAATGCGAGACTTCAGGTGAACCAGCATAGTTGCATGCAATATCGCATAGCCCTAGGCATATACTATTTGGGTGTGAACGTGGGGTACAGGATGTTCCACTTCAATCCCTACAGACATGTAATAATCATAAAAATCATTAGCTTAATAATTTGTGCTAGGAGTTTCGCAAATGCAGCATTGCATGTGGACAACAGGCAAACATGTGACCATCGTGTCGATGCATCAACCAACACCATAAAGTATCAAAATGGTCCACATGTTGGTTGGATAGGGGTTGTGAATAATCTTTGTAATCAAGGGTTGAGTATTCAACTTCCCCAAAGAACACACTTTGCATGGTGGGAATCCAACGGCGTATCATATCACATCCGAGATGTCCCATACGGTAATGCCAAAGTAGCAAAGTACTCTGGAACTCAGGCACAGGGCTGGCCACATGAGAAGTTCCAACTTCTCGTGAATACGCTTCTGGCCATATTCGTACGAAGTGGTACAAAGAAATTTAGAACCATTATCTTCAGTGGTTTCAACATGATATTGATTATCTCAAATATCTCTAAAACTCAGATTAAGACAATACCATTGGACAACATGATACAGGTCTTTCCGTATCCTTCAATCAGGTTTGATGGGCCTGAGAGATGTCACTCATGCAATATGGTGTGTGTGGTAGCACTATCAGCCAGACAACTAACTTCCCCACTACCTAGAAATAAATTGATTGAATTGGTCACATGCATAAATATAATTCCATTCACTCAATTAATCAATTCGAGAAATAATATCCATAAAATAACAATCCATAATTCAAAACAAACCAAAACTAAACAAATTATTCCAAATACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

619

Amino Acids

71.43

Weight (kDa)

9.78

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020428)

Species Orthologous Gene IDs
pyrus_communis pycom01g12280 pycom10g03800 pycom13g24390 pycom14g14160
rosa_chinensis RchiOBHm_Chr5g0014091
rosa_samantha Rh5CG118100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1220
Acc36I ACCTGC 1 cut(s) 636
AccB1I GGYRCC 1 cut(s) 544
AccB7I CCANNNNNTGG 2 cut(s) 112, 1268
AccI GTMKAC 1 cut(s) 359
AccII CGCG 1 cut(s) 224
AciI CCGC 1 cut(s) 968
AcoI YGGCCR 2 cut(s) 1439, 1479
AcsI RAATTY 1 cut(s) 1508
AcuI CTGAAG 5 cut(s) 585, 784, 797, 1004, 1510
AcvI CACGTG 1 cut(s) 1004
AfiI CCNNNNNNNGG 4 cut(s) 112, 561, 880, 1268
AflIII ACRYGT 5 cut(s) 1001, 1003, 1123, 1208, 1264
AluBI AGCT 2 cut(s) 414, 1149
AluI AGCT 2 cut(s) 414, 1149
Alw26I GTCTC 2 cut(s) 221, 1009
Ama87I CYCGRG 1 cut(s) 506
AoxI GGCC 4 cut(s) 676, 1439, 1479, 1640
ApeKI GCWGC 1 cut(s) 1181
ApoI RAATTY 1 cut(s) 1508
AseI ATTAAT 1 cut(s) 1772
Asp700I GAANNNNTTC 2 cut(s) 79, 120
AspA2I CCTAGG 1 cut(s) 1060
AspS9I GGNCC 5 cut(s) 324, 487, 946, 1259, 1640
AsuC2I CCSGG 1 cut(s) 562
AsuHPI GGTGA 1 cut(s) 1036
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 4 cut(s) 324, 487, 946, 1259
AvrII CCTAGG 1 cut(s) 1060
BalI TGGCCA 2 cut(s) 1441, 1481
BanI GGYRCC 1 cut(s) 544
BauI CACGAG 2 cut(s) 497, 1463
BbrPI CACGTG 1 cut(s) 1004
BbsI GAAGAC 3 cut(s) 537, 680, 872
BbvI GCAGC 1 cut(s) 1193
BccI CCATC 3 cut(s) 898, 1224, 1631
BceAI ACGGC 1 cut(s) 1375
BciVI GTATCC 1 cut(s) 1629
BcnI CCSGG 1 cut(s) 562
BcoDI GTCTC 2 cut(s) 221, 1009
BfaI CTAG 7 cut(s) 152, 684, 827, 855, 1061, 1164, 1716
BfmI CTRYAG 2 cut(s) 319, 1117
BfuAI ACCTGC 1 cut(s) 636
BfuI GTATCC 1 cut(s) 1629
BglII AGATCT 2 cut(s) 154, 780
BisI GCNGC 2 cut(s) 969, 1182
BlnI CCTAGG 1 cut(s) 1060
BlsI GCNGC 2 cut(s) 970, 1183
BmcAI AGTACT 1 cut(s) 1415
Bme1390I CCNGG 1 cut(s) 562
Bme18I GGWCC 4 cut(s) 324, 487, 946, 1259
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 5 cut(s) 324, 487, 946, 1259, 1640
BmiI GGNNCC 2 cut(s) 546, 948
BmrFI CCNGG 1 cut(s) 562
BmsI GCATC 3 cut(s) 441, 1216, 1238
BpiI GAAGAC 3 cut(s) 537, 680, 872
BplI GAGNNNNNCTC 2 cut(s) 757, 789
BpuMI CCSGG 1 cut(s) 562
BsaAI YACGTR 2 cut(s) 961, 1004
BsaJI CCNNGG 3 cut(s) 540, 561, 1060
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 561, 880, 1268
Bse3DI GCAATG 2 cut(s) 775, 1184
BseDI CCNNGG 3 cut(s) 540, 561, 1060
BseGI GGATG 3 cut(s) 468, 1104, 1374
BseLI CCNNNNNNNGG 4 cut(s) 112, 561, 880, 1268
BseMI GCAATG 2 cut(s) 775, 1184
BseMII CTCAG 3 cut(s) 1439, 1586, 1635
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 1 cut(s) 1193
BsgI GTGCAG 1 cut(s) 974
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 4 cut(s) 678, 1441, 1481, 1642
BshNI GGYRCC 1 cut(s) 544
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 1 cut(s) 561
BsiWI CGTACG 1 cut(s) 1489
BslFI GGGAC 1 cut(s) 1370
BslI CCNNNNNNNGG 4 cut(s) 112, 561, 880, 1268
BsmAI GTCTC 2 cut(s) 221, 1009
BsmFI GGGAC 1 cut(s) 1370
BsnI GGCC 4 cut(s) 678, 1441, 1481, 1642
BsoBI CYCGRG 1 cut(s) 506
Bsp1407I TGTACA 1 cut(s) 196
Bsp143I GATC 2 cut(s) 154, 780
BspACI CCGC 1 cut(s) 968
BspANI GGCC 4 cut(s) 678, 1441, 1481, 1642
BspCNI CTCAG 3 cut(s) 1438, 1585, 1636
BspFNI CGCG 1 cut(s) 224
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 2 cut(s) 546, 948
BspMI ACCTGC 1 cut(s) 636
BspT107I GGYRCC 1 cut(s) 544
BsrDI GCAATG 2 cut(s) 775, 1184
BsrGI TGTACA 1 cut(s) 196
BssECI CCNNGG 3 cut(s) 540, 561, 1060
BssMI GATC 2 cut(s) 154, 780
BssSI CACGAG 2 cut(s) 497, 1463
BssT1I CCWWGG 2 cut(s) 540, 1060
Bst2BI CACGAG 2 cut(s) 497, 1463
Bst4CI ACNGT 2 cut(s) 419, 1394
Bst6I CTCTTC 2 cut(s) 562, 604
BstAUI TGTACA 1 cut(s) 196
BstBAI YACGTR 2 cut(s) 961, 1004
BstC8I GCNNGC 4 cut(s) 226, 841, 1043, 1439
BstDEI CTNAG 6 cut(s) 396, 942, 1425, 1572, 1644, 1854
BstF5I GGATG 3 cut(s) 468, 1104, 1374
BstFNI CGCG 1 cut(s) 224
BstKTI GATC 2 cut(s) 157, 783
BstMAI GTCTC 2 cut(s) 221, 1009
BstMBI GATC 2 cut(s) 154, 780
BstMWI GCNNNNNNNGC 4 cut(s) 977, 1181, 1406, 1688
BstNSI RCATGY 8 cut(s) 737, 843, 1045, 1127, 1193, 1212, 1268, 1746
BstSCI CCNGG 1 cut(s) 560
BstSFI CTRYAG 2 cut(s) 319, 1117
BstSNI TACGTA 1 cut(s) 961
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 1 cut(s) 1193
BstV2I GAAGAC 3 cut(s) 537, 680, 872
BstX2I RGATCY 2 cut(s) 154, 780
BstYI RGATCY 2 cut(s) 154, 780
BsuI GTATCC 1 cut(s) 1629
BsuRI GGCC 4 cut(s) 678, 1441, 1481, 1642
BtsCI GGATG 3 cut(s) 468, 1104, 1374
BtsIMutI CAGTG 1 cut(s) 1534
BveI ACCTGC 1 cut(s) 636
Cac8I GCNNGC 4 cut(s) 226, 841, 1043, 1439
CciI TCATGA 1 cut(s) 268
Cfr13I GGNCC 5 cut(s) 324, 487, 946, 1259, 1640
DdeI CTNAG 6 cut(s) 396, 942, 1425, 1572, 1644, 1854
DpnI GATC 2 cut(s) 156, 782
DpnII GATC 2 cut(s) 154, 780
DraI TTTAAA 1 cut(s) 745
DrdI GACNNNNNNGTC 1 cut(s) 1220
DseDI GACNNNNNNGTC 1 cut(s) 1220
EaeI YGGCCR 2 cut(s) 1439, 1479
Eam1104I CTCTTC 2 cut(s) 562, 604
EarI CTCTTC 2 cut(s) 562, 604
Eco105I TACGTA 1 cut(s) 961
Eco130I CCWWGG 2 cut(s) 540, 1060
Eco147I AGGCCT 1 cut(s) 678
Eco47I GGWCC 4 cut(s) 324, 487, 946, 1259
Eco57I CTGAAG 5 cut(s) 585, 784, 797, 1004, 1510
Eco72I CACGTG 1 cut(s) 1004
Eco88I CYCGRG 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 946
EcoT14I CCWWGG 2 cut(s) 540, 1060
EcoT22I ATGCAT 3 cut(s) 434, 1231, 1748
ErhI CCWWGG 2 cut(s) 540, 1060
FaqI GGGAC 1 cut(s) 1370
FauNDI CATATG 1 cut(s) 302
FblI GTMKAC 1 cut(s) 359
Fnu4HI GCNGC 2 cut(s) 969, 1182
FokI GGATG 3 cut(s) 455, 1111, 1361
Fsp4HI GCNGC 2 cut(s) 969, 1182
FspBI CTAG 7 cut(s) 152, 684, 827, 855, 1061, 1164, 1716
GluI GCNGC 2 cut(s) 969, 1182
HaeIII GGCC 4 cut(s) 678, 1441, 1481, 1642
HapII CCGG 1 cut(s) 561
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HinfI GANTC 7 cut(s) 457, 502, 571, 868, 910, 927, 1352
HpaII CCGG 1 cut(s) 561
HphI GGTGA 1 cut(s) 1036
Hpy166II GTNNAC 9 cut(s) 360, 422, 487, 592, 896, 1027, 1084, 1195, 1262
Hpy188I TCNGA 5 cut(s) 456, 610, 777, 1379, 1575
Hpy188III TCNNGA 9 cut(s) 125, 152, 269, 499, 506, 508, 1419, 1465, 1782
Hpy8I GTNNAC 9 cut(s) 360, 422, 487, 592, 896, 1027, 1084, 1195, 1262
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 2 cut(s) 131, 1632
HpyCH4III ACNGT 2 cut(s) 419, 1394
HpyCH4IV ACGT 7 cut(s) 338, 356, 708, 721, 960, 1003, 1086
HpyF10VI GCNNNNNNNGC 4 cut(s) 977, 1181, 1406, 1688
HpyF3I CTNAG 6 cut(s) 396, 942, 1425, 1572, 1644, 1854
HpySE526I ACGT 7 cut(s) 338, 356, 708, 721, 960, 1003, 1086
Kzo9I GATC 2 cut(s) 154, 780
LmnI GCTCC 1 cut(s) 610
Lsp1109I GCAGC 1 cut(s) 1193
LweI GCATC 3 cut(s) 441, 1216, 1238
MaeI CTAG 7 cut(s) 152, 684, 827, 855, 1061, 1164, 1716
MaeII ACGT 7 cut(s) 338, 356, 708, 721, 960, 1003, 1086
MaeIII GTNAC 5 cut(s) 174, 792, 1211, 1652, 1738
MalI GATC 2 cut(s) 156, 782
MboI GATC 2 cut(s) 154, 780
MboII GAAGA 8 cut(s) 242, 542, 549, 591, 680, 790, 877, 1516
MfeI CAATTG 1 cut(s) 899
MflI RGATCY 2 cut(s) 154, 780
MlsI TGGCCA 2 cut(s) 1441, 1481
MluNI TGGCCA 2 cut(s) 1441, 1481
MlyI GAGTC 1 cut(s) 862
MmeI TCCRAC 4 cut(s) 588, 1253, 1380, 1480
MnlI CCTC 8 cut(s) 230, 276, 476, 504, 565, 672, 709, 862
Mox20I TGGCCA 2 cut(s) 1441, 1481
Mph1103I ATGCAT 3 cut(s) 434, 1231, 1748
MroXI GAANNNNTTC 2 cut(s) 79, 120
MscI TGGCCA 2 cut(s) 1441, 1481
MseI TTAA 4 cut(s) 744, 1151, 1578, 1772
MslI CAYNNNNRTG 2 cut(s) 51, 838
Msp20I TGGCCA 2 cut(s) 1441, 1481
MspI CCGG 1 cut(s) 561
MspR9I CCNGG 1 cut(s) 562
MunI CAATTG 1 cut(s) 899
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 4 cut(s) 977, 1181, 1406, 1688
NciI CCSGG 1 cut(s) 562
NdeI CATATG 1 cut(s) 302
NdeII GATC 2 cut(s) 154, 780
NlaIV GGNNCC 2 cut(s) 546, 948
NmuCI GTSAC 4 cut(s) 792, 1211, 1652, 1738
NsiI ATGCAT 3 cut(s) 434, 1231, 1748
NspI RCATGY 8 cut(s) 737, 843, 1045, 1127, 1193, 1212, 1268, 1746
PaeI GCATGC 2 cut(s) 843, 1045
PaeR7I CTCGAG 1 cut(s) 506
PagI TCATGA 1 cut(s) 268
PceI AGGCCT 1 cut(s) 678
PciI ACATGT 3 cut(s) 1123, 1208, 1264
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PdmI GAANNNNTTC 2 cut(s) 79, 120
PfeI GAWTC 6 cut(s) 457, 502, 571, 910, 927, 1352
Pfl23II CGTACG 1 cut(s) 1489
PflMI CCANNNNNTGG 2 cut(s) 112, 1268
PkrI GCNGC 2 cut(s) 970, 1183
PleI GAGTC 1 cut(s) 862
PmaCI CACGTG 1 cut(s) 1004
PmlI CACGTG 1 cut(s) 1004
PpsI GAGTC 1 cut(s) 862
Ppu21I YACGTR 2 cut(s) 961, 1004
PpuMI RGGWCCY 1 cut(s) 946
PscI ACATGT 3 cut(s) 1123, 1208, 1264
PshBI ATTAAT 1 cut(s) 1772
Psp5II RGGWCCY 1 cut(s) 946
PspCI CACGTG 1 cut(s) 1004
PspLI CGTACG 1 cut(s) 1489
PspN4I GGNNCC 2 cut(s) 546, 948
PspPI GGNCC 5 cut(s) 324, 487, 946, 1259, 1640
PspPPI RGGWCCY 1 cut(s) 946
PsrI GAACNNNNNNTAC 4 cut(s) 1076, 1085, 1108, 1117
PsuI RGATCY 2 cut(s) 154, 780
RseI CAYNNNNRTG 2 cut(s) 51, 838
SalI GTCGAC 1 cut(s) 358
SaqAI TTAA 4 cut(s) 744, 1151, 1578, 1772
SatI GCNGC 2 cut(s) 969, 1182
Sau3AI GATC 2 cut(s) 154, 780
Sau96I GGNCC 5 cut(s) 324, 487, 946, 1259, 1640
ScaI AGTACT 1 cut(s) 1415
SchI GAGTC 1 cut(s) 862
ScrFI CCNGG 1 cut(s) 562
SfaNI GCATC 3 cut(s) 441, 1216, 1238
SfcI CTRYAG 2 cut(s) 319, 1117
Sfr274I CTCGAG 1 cut(s) 506
SinI GGWCC 4 cut(s) 324, 487, 946, 1259
SlaI CTCGAG 1 cut(s) 506
SmiMI CAYNNNNRTG 2 cut(s) 51, 838
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SnaBI TACGTA 1 cut(s) 961
SphI GCATGC 2 cut(s) 843, 1045
SseBI AGGCCT 1 cut(s) 678
SsiI CCGC 1 cut(s) 968
SspI AATATT 2 cut(s) 43, 476
SspMI CTAG 7 cut(s) 152, 684, 827, 855, 1061, 1164, 1716
StuI AGGCCT 1 cut(s) 678
StyD4I CCNGG 1 cut(s) 560
StyI CCWWGG 2 cut(s) 540, 1060
TaaI ACNGT 2 cut(s) 419, 1394
TaiI ACGT 7 cut(s) 341, 359, 711, 724, 963, 1006, 1089
TaqI TCGA 6 cut(s) 359, 438, 507, 528, 1224, 1781
TaqII GACCGA 1 cut(s) 504
TatI WGTACW 3 cut(s) 196, 655, 1413
TauI GCSGC 1 cut(s) 971
TfiI GAWTC 6 cut(s) 457, 502, 571, 910, 927, 1352
Tru1I TTAA 4 cut(s) 744, 1151, 1578, 1772
Tru9I TTAA 4 cut(s) 744, 1151, 1578, 1772
TscAI CASTG 1 cut(s) 1534
TseFI GTSAC 4 cut(s) 792, 1211, 1652, 1738
TseI GCWGC 1 cut(s) 1181
Tsp45I GTSAC 4 cut(s) 792, 1211, 1652, 1738
TspDTI ATGAA 6 cut(s) 123, 235, 285, 355, 387, 878
TspGWI ACGGA 2 cut(s) 135, 1605
TspRI CASTG 1 cut(s) 1534
Van91I CCANNNNNTGG 2 cut(s) 112, 1268
VpaK11BI GGWCC 4 cut(s) 324, 487, 946, 1259
VspI ATTAAT 1 cut(s) 1772
XapI RAATTY 1 cut(s) 1508
XbaI TCTAGA 1 cut(s) 151
XceI RCATGY 8 cut(s) 737, 843, 1045, 1127, 1193, 1212, 1268, 1746
XcmI CCANNNNNNNNNTGG 1 cut(s) 1269
XhoI CTCGAG 1 cut(s) 506
XmaJI CCTAGG 1 cut(s) 1060
XmiI GTMKAC 1 cut(s) 359
XmnI GAANNNNTTC 2 cut(s) 79, 120
XspI CTAG 7 cut(s) 152, 684, 827, 855, 1061, 1164, 1716
ZrmI AGTACT 1 cut(s) 1415
Zsp2I ATGCAT 3 cut(s) 434, 1231, 1748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.