Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| malus_domestica | MD13G1240700.v1.1 MD16G1133500.v1.1 |
| pyrus_communis | pycom13g27230 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 215 |
| AccBSI | CCGCTC | 1 cut(s) | 1476 |
| AccI | GTMKAC | 2 cut(s) | 1020, 2642 |
| AccII | CGCG | 2 cut(s) | 3074, 3080 |
| AciI | CCGC | 5 cut(s) | 122, 1476, 2349, 3080, 3318 |
| AclWI | GGATC | 1 cut(s) | 3432 |
| AcoI | YGGCCR | 4 cut(s) | 945, 3189, 3315, 3501 |
| AcuI | CTGAAG | 2 cut(s) | 557, 3395 |
| AcvI | CACGTG | 1 cut(s) | 2744 |
| AfaI | GTAC | 7 cut(s) | 419, 2149, 2309, 2463, 2478, 2676, 3077 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 2348, 3324 |
| AflII | CTTAAG | 1 cut(s) | 452 |
| AflIII | ACRYGT | 1 cut(s) | 2743 |
| AgeI | ACCGGT | 1 cut(s) | 3272 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 864 |
| AjnI | CCWGG | 2 cut(s) | 1109, 1509 |
| Alw21I | GWGCWC | 2 cut(s) | 2743, 3205 |
| Alw26I | GTCTC | 5 cut(s) | 1063, 1409, 1704, 2130, 3308 |
| Alw44I | GTGCAC | 1 cut(s) | 3201 |
| AlwI | GGATC | 1 cut(s) | 3432 |
| Ama87I | CYCGRG | 1 cut(s) | 752 |
| AoxI | GGCC | 6 cut(s) | 945, 2896, 3189, 3315, 3414, 3501 |
| ApaLI | GTGCAC | 1 cut(s) | 3201 |
| ApeKI | GCWGC | 2 cut(s) | 674, 1532 |
| AseI | ATTAAT | 1 cut(s) | 2027 |
| AsiGI | ACCGGT | 1 cut(s) | 3272 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 8, 537 |
| AspLEI | GCGC | 1 cut(s) | 3074 |
| AsuHPI | GGTGA | 4 cut(s) | 708, 2045, 2510, 2771 |
| AsuII | TTCGAA | 2 cut(s) | 503, 2926 |
| AsuNHI | GCTAGC | 2 cut(s) | 3208, 3230 |
| AvaI | CYCGRG | 1 cut(s) | 752 |
| AxyI | CCTNAGG | 1 cut(s) | 2706 |
| BaeGI | GKGCMC | 2 cut(s) | 1890, 3205 |
| BanI | GGYRCC | 1 cut(s) | 215 |
| BanII | GRGCYC | 1 cut(s) | 2107 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 673, 705 |
| BbrPI | CACGTG | 1 cut(s) | 2744 |
| BbsI | GAAGAC | 2 cut(s) | 1431, 3380 |
| Bbv12I | GWGCWC | 2 cut(s) | 2743, 3205 |
| BbvI | GCAGC | 2 cut(s) | 686, 1544 |
| BccI | CCATC | 5 cut(s) | 277, 370, 1473, 1601, 3288 |
| BceAI | ACGGC | 4 cut(s) | 915, 960, 3204, 3488 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1524, 1558, 3182, 3216 |
| BciT130I | CCWGG | 2 cut(s) | 1111, 1511 |
| BclI | TGATCA | 1 cut(s) | 777 |
| BcoDI | GTCTC | 5 cut(s) | 1063, 1409, 1704, 2130, 3308 |
| BfaI | CTAG | 8 cut(s) | 366, 2015, 2327, 2426, 2430, 3209, 3222, 3231 |
| BfmI | CTRYAG | 1 cut(s) | 679 |
| BfrI | CTTAAG | 1 cut(s) | 452 |
| BisI | GCNGC | 3 cut(s) | 675, 1533, 3318 |
| BlsI | GCNGC | 3 cut(s) | 676, 1534, 3319 |
| Bme1390I | CCNGG | 2 cut(s) | 1111, 1511 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 864 |
| BmeT110I | CYCGRG | 1 cut(s) | 752 |
| BmiI | GGNNCC | 1 cut(s) | 217 |
| BmrFI | CCNGG | 2 cut(s) | 1111, 1511 |
| BmrI | ACTGGG | 1 cut(s) | 439 |
| BmsI | GCATC | 5 cut(s) | 123, 1110, 1377, 2610, 3181 |
| BmtI | GCTAGC | 2 cut(s) | 3212, 3234 |
| BmuI | ACTGGG | 1 cut(s) | 439 |
| BpiI | GAAGAC | 2 cut(s) | 1431, 3380 |
| BpmI | CTGGAG | 2 cut(s) | 1391, 1532 |
| Bpu14I | TTCGAA | 2 cut(s) | 503, 2926 |
| BpuEI | CTTGAG | 1 cut(s) | 2140 |
| Bsa29I | ATCGAT | 2 cut(s) | 763, 958 |
| BsaAI | YACGTR | 1 cut(s) | 2744 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1796 |
| BsaI | GGTCTC | 1 cut(s) | 1409 |
| BsaJI | CCNNGG | 3 cut(s) | 1110, 3240, 3480 |
| BsaWI | WCCGGW | 1 cut(s) | 3272 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 256, 286 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 2348, 3324 |
| Bse118I | RCCGGY | 2 cut(s) | 212, 3272 |
| Bse1I | ACTGG | 5 cut(s) | 106, 445, 642, 1408, 2833 |
| Bse21I | CCTNAGG | 1 cut(s) | 2706 |
| Bse3DI | GCAATG | 2 cut(s) | 3007, 3456 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1796 |
| BseBI | CCWGG | 2 cut(s) | 1111, 1511 |
| BseCI | ATCGAT | 2 cut(s) | 763, 958 |
| BseDI | CCNNGG | 3 cut(s) | 1110, 3240, 3480 |
| BseGI | GGATG | 4 cut(s) | 244, 887, 1484, 2896 |
| BseJI | GATNNNNATC | 1 cut(s) | 1796 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 2348, 3324 |
| BseMI | GCAATG | 2 cut(s) | 3007, 3456 |
| BseMII | CTCAG | 4 cut(s) | 1074, 1405, 3360, 3372 |
| BseNI | ACTGG | 5 cut(s) | 106, 445, 642, 1408, 2833 |
| BseRI | GAGGAG | 1 cut(s) | 720 |
| BseSI | GKGCMC | 2 cut(s) | 1890, 3205 |
| BseX3I | CGGCCG | 1 cut(s) | 3189 |
| BseXI | GCAGC | 2 cut(s) | 686, 1544 |
| BsgI | GTGCAG | 1 cut(s) | 346 |
| Bsh1236I | CGCG | 2 cut(s) | 3074, 3080 |
| Bsh1285I | CGRYCG | 2 cut(s) | 767, 3192 |
| BshFI | GGCC | 6 cut(s) | 947, 2898, 3191, 3317, 3416, 3503 |
| BshNI | GGYRCC | 1 cut(s) | 215 |
| BshTI | ACCGGT | 1 cut(s) | 3272 |
| BshVI | ATCGAT | 2 cut(s) | 763, 958 |
| BsiEI | CGRYCG | 2 cut(s) | 767, 3192 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2743, 3205 |
| BsiHKCI | CYCGRG | 1 cut(s) | 752 |
| BsiSI | CCGG | 2 cut(s) | 213, 3273 |
| BsiWI | CGTACG | 1 cut(s) | 3075 |
| BslFI | GGGAC | 1 cut(s) | 14 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 2348, 3324 |
| BsmAI | GTCTC | 5 cut(s) | 1063, 1409, 1704, 2130, 3308 |
| BsmFI | GGGAC | 1 cut(s) | 14 |
| BsmI | GAATGC | 1 cut(s) | 932 |
| BsnI | GGCC | 6 cut(s) | 947, 2898, 3191, 3317, 3416, 3503 |
| Bso31I | GGTCTC | 1 cut(s) | 1409 |
| BsoBI | CYCGRG | 1 cut(s) | 752 |
| Bsp119I | TTCGAA | 2 cut(s) | 503, 2926 |
| Bsp1286I | GDGCHC | 4 cut(s) | 1890, 2107, 2743, 3205 |
| Bsp1407I | TGTACA | 1 cut(s) | 2307 |
| Bsp143I | GATC | 7 cut(s) | 204, 764, 777, 874, 916, 3424, 3472 |
| BspACI | CCGC | 5 cut(s) | 122, 1476, 2349, 3080, 3318 |
| BspANI | GGCC | 6 cut(s) | 947, 2898, 3191, 3317, 3416, 3503 |
| BspCNI | CTCAG | 4 cut(s) | 1073, 1404, 3361, 3371 |
| BspDI | ATCGAT | 2 cut(s) | 763, 958 |
| BspFNI | CGCG | 2 cut(s) | 3074, 3080 |
| BspHI | TCATGA | 1 cut(s) | 2604 |
| BspLI | GGNNCC | 1 cut(s) | 217 |
| BspOI | GCTAGC | 2 cut(s) | 3212, 3234 |
| BspPI | GGATC | 1 cut(s) | 3432 |
| BspT104I | TTCGAA | 2 cut(s) | 503, 2926 |
| BspT107I | GGYRCC | 1 cut(s) | 215 |
| BspTI | CTTAAG | 1 cut(s) | 452 |
| BspTNI | GGTCTC | 1 cut(s) | 1409 |
| BsrBI | CCGCTC | 1 cut(s) | 1476 |
| BsrDI | GCAATG | 2 cut(s) | 3007, 3456 |
| BsrFI | RCCGGY | 2 cut(s) | 212, 3272 |
| BsrGI | TGTACA | 1 cut(s) | 2307 |
| BsrI | ACTGG | 5 cut(s) | 106, 445, 642, 1408, 2833 |
| BssAI | RCCGGY | 2 cut(s) | 212, 3272 |
| BssECI | CCNNGG | 3 cut(s) | 1110, 3240, 3480 |
| BssMI | GATC | 7 cut(s) | 204, 764, 777, 874, 916, 3424, 3472 |
| BssNAI | GTATAC | 1 cut(s) | 2643 |
| BssT1I | CCWWGG | 2 cut(s) | 3240, 3480 |
| Bst1107I | GTATAC | 1 cut(s) | 2643 |
| Bst2UI | CCWGG | 2 cut(s) | 1111, 1511 |
| Bst4CI | ACNGT | 4 cut(s) | 318, 440, 1636, 3292 |
| Bst6I | CTCTTC | 6 cut(s) | 100, 120, 1148, 1353, 1512, 3168 |
| BstAFI | CTTAAG | 1 cut(s) | 452 |
| BstAUI | TGTACA | 1 cut(s) | 2307 |
| BstBAI | YACGTR | 1 cut(s) | 2744 |
| BstBI | TTCGAA | 2 cut(s) | 503, 2926 |
| BstC8I | GCNNGC | 7 cut(s) | 214, 329, 353, 2991, 3210, 3232, 3435 |
| BstDEI | CTNAG | 9 cut(s) | 482, 737, 1060, 1391, 1990, 2694, 2706, 3358, 3369 |
| BstEII | GGTNACC | 1 cut(s) | 3275 |
| BstF5I | GGATG | 4 cut(s) | 244, 887, 1484, 2896 |
| BstFNI | CGCG | 2 cut(s) | 3074, 3080 |
| BstHHI | GCGC | 1 cut(s) | 3074 |
| BstKTI | GATC | 7 cut(s) | 207, 767, 780, 877, 919, 3427, 3475 |
| BstMAI | GTCTC | 5 cut(s) | 1063, 1409, 1704, 2130, 3308 |
| BstMBI | GATC | 7 cut(s) | 204, 764, 777, 874, 916, 3424, 3472 |
| BstMCI | CGRYCG | 2 cut(s) | 767, 3192 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 348, 3209, 3413 |
| BstNI | CCWGG | 2 cut(s) | 1111, 1511 |
| BstNSI | RCATGY | 2 cut(s) | 2013, 2349 |
| BstPI | GGTNACC | 1 cut(s) | 3275 |
| BstSCI | CCNGG | 2 cut(s) | 1109, 1509 |
| BstSFI | CTRYAG | 1 cut(s) | 679 |
| BstSLI | GKGCMC | 2 cut(s) | 1890, 3205 |
| BstUI | CGCG | 2 cut(s) | 3074, 3080 |
| BstV1I | GCAGC | 2 cut(s) | 686, 1544 |
| BstV2I | GAAGAC | 2 cut(s) | 1431, 3380 |
| BstZ17I | GTATAC | 1 cut(s) | 2643 |
| BstZI | CGGCCG | 1 cut(s) | 3189 |
| Bsu15I | ATCGAT | 2 cut(s) | 763, 958 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2706 |
| BsuRI | GGCC | 6 cut(s) | 947, 2898, 3191, 3317, 3416, 3503 |
| BsuTUI | ATCGAT | 2 cut(s) | 763, 958 |
| BtsCI | GGATG | 4 cut(s) | 244, 887, 1484, 2896 |
| BtsI | GCAGTG | 1 cut(s) | 3203 |
| BtsIMutI | CAGTG | 4 cut(s) | 2367, 3203, 3249, 3264 |
| Cac8I | GCNNGC | 7 cut(s) | 214, 329, 353, 2991, 3210, 3232, 3435 |
| CciI | TCATGA | 1 cut(s) | 2604 |
| CfoI | GCGC | 1 cut(s) | 3074 |
| Cfr10I | RCCGGY | 2 cut(s) | 212, 3272 |
| ClaI | ATCGAT | 2 cut(s) | 763, 958 |
| Csp6I | GTAC | 7 cut(s) | 418, 2148, 2308, 2462, 2477, 2675, 3076 |
| CspAI | ACCGGT | 1 cut(s) | 3272 |
| CviQI | GTAC | 7 cut(s) | 418, 2148, 2308, 2462, 2477, 2675, 3076 |
| DdeI | CTNAG | 9 cut(s) | 482, 737, 1060, 1391, 1990, 2694, 2706, 3358, 3369 |
| DpnI | GATC | 7 cut(s) | 206, 766, 779, 876, 918, 3426, 3474 |
| DpnII | GATC | 7 cut(s) | 204, 764, 777, 874, 916, 3424, 3472 |
| DraI | TTTAAA | 1 cut(s) | 2214 |
| DriI | GACNNNNNGTC | 1 cut(s) | 864 |
| EaeI | YGGCCR | 4 cut(s) | 945, 3189, 3315, 3501 |
| EagI | CGGCCG | 1 cut(s) | 3189 |
| Eam1104I | CTCTTC | 6 cut(s) | 100, 120, 1148, 1353, 1512, 3168 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 864 |
| EarI | CTCTTC | 6 cut(s) | 100, 120, 1148, 1353, 1512, 3168 |
| EclXI | CGGCCG | 1 cut(s) | 3189 |
| Eco130I | CCWWGG | 2 cut(s) | 3240, 3480 |
| Eco24I | GRGCYC | 1 cut(s) | 2107 |
| Eco31I | GGTCTC | 1 cut(s) | 1409 |
| Eco32I | GATATC | 1 cut(s) | 1996 |
| Eco52I | CGGCCG | 1 cut(s) | 3189 |
| Eco57I | CTGAAG | 2 cut(s) | 557, 3395 |
| Eco72I | CACGTG | 1 cut(s) | 2744 |
| Eco81I | CCTNAGG | 1 cut(s) | 2706 |
| Eco88I | CYCGRG | 1 cut(s) | 752 |
| Eco91I | GGTNACC | 1 cut(s) | 3275 |
| EcoO65I | GGTNACC | 1 cut(s) | 3275 |
| EcoRI | GAATTC | 3 cut(s) | 4, 505, 1588 |
| EcoRII | CCWGG | 2 cut(s) | 1109, 1509 |
| EcoRV | GATATC | 1 cut(s) | 1996 |
| EcoT14I | CCWWGG | 2 cut(s) | 3240, 3480 |
| EcoT22I | ATGCAT | 2 cut(s) | 1251, 2603 |
| EcoT38I | GRGCYC | 1 cut(s) | 2107 |
| ErhI | CCWWGG | 2 cut(s) | 3240, 3480 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 525, 557, 1973, 2005 |
| FaqI | GGGAC | 1 cut(s) | 14 |
| FbaI | TGATCA | 1 cut(s) | 777 |
| FblI | GTMKAC | 2 cut(s) | 1020, 2642 |
| Fnu4HI | GCNGC | 3 cut(s) | 675, 1533, 3318 |
| FokI | GGATG | 4 cut(s) | 231, 894, 1491, 2903 |
| FriOI | GRGCYC | 1 cut(s) | 2107 |
| Fsp4HI | GCNGC | 3 cut(s) | 675, 1533, 3318 |
| FspBI | CTAG | 8 cut(s) | 366, 2015, 2327, 2426, 2430, 3209, 3222, 3231 |
| GlaI | GCGC | 1 cut(s) | 3073 |
| GluI | GCNGC | 3 cut(s) | 675, 1533, 3318 |
| GsuI | CTGGAG | 2 cut(s) | 1391, 1532 |
| HaeIII | GGCC | 6 cut(s) | 947, 2898, 3191, 3317, 3416, 3503 |
| HapII | CCGG | 2 cut(s) | 213, 3273 |
| HhaI | GCGC | 1 cut(s) | 3074 |
| Hin6I | GCGC | 1 cut(s) | 3072 |
| HinP1I | GCGC | 1 cut(s) | 3072 |
| HincII | GTYRAC | 1 cut(s) | 1204 |
| HindII | GTYRAC | 1 cut(s) | 1204 |
| HindIII | AAGCTT | 2 cut(s) | 534, 546 |
| HpaII | CCGG | 2 cut(s) | 213, 3273 |
| HphI | GGTGA | 4 cut(s) | 708, 2045, 2510, 2771 |
| Hpy188I | TCNGA | 5 cut(s) | 385, 591, 894, 1450, 3477 |
| Hpy188III | TCNNGA | 7 cut(s) | 702, 752, 2539, 2605, 3222, 3268, 3428 |
| HpyAV | CCTTC | 8 cut(s) | 68, 258, 382, 1437, 2869, 3189, 3317, 3415 |
| HpyCH4III | ACNGT | 4 cut(s) | 318, 440, 1636, 3292 |
| HpyCH4IV | ACGT | 5 cut(s) | 160, 183, 1935, 2239, 2743 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 348, 3209, 3413 |
| HpyF3I | CTNAG | 9 cut(s) | 482, 737, 1060, 1391, 1990, 2694, 2706, 3358, 3369 |
| HpySE526I | ACGT | 5 cut(s) | 160, 183, 1935, 2239, 2743 |
| HspAI | GCGC | 1 cut(s) | 3072 |
| KroI | GCCGGC | 1 cut(s) | 212 |
| KroNI | GCCGGC | 1 cut(s) | 214 |
| Ksp22I | TGATCA | 1 cut(s) | 777 |
| Kzo9I | GATC | 7 cut(s) | 204, 764, 777, 874, 916, 3424, 3472 |
| LmnI | GCTCC | 1 cut(s) | 1473 |
| Lsp1109I | GCAGC | 2 cut(s) | 686, 1544 |
| LweI | GCATC | 5 cut(s) | 123, 1110, 1377, 2610, 3181 |
| MaeI | CTAG | 8 cut(s) | 366, 2015, 2327, 2426, 2430, 3209, 3222, 3231 |
| MaeII | ACGT | 5 cut(s) | 160, 183, 1935, 2239, 2743 |
| MaeIII | GTNAC | 4 cut(s) | 1636, 2421, 2777, 3275 |
| MalI | GATC | 7 cut(s) | 206, 766, 779, 876, 918, 3426, 3474 |
| MbiI | CCGCTC | 1 cut(s) | 1476 |
| MboI | GATC | 7 cut(s) | 204, 764, 777, 874, 916, 3424, 3472 |
| MhlI | GDGCHC | 4 cut(s) | 1890, 2107, 2743, 3205 |
| Mph1103I | ATGCAT | 2 cut(s) | 1251, 2603 |
| MroNI | GCCGGC | 1 cut(s) | 212 |
| MroXI | GAANNNNTTC | 2 cut(s) | 8, 537 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1014, 2852 |
| MspCI | CTTAAG | 1 cut(s) | 452 |
| MspI | CCGG | 2 cut(s) | 213, 3273 |
| MspR9I | CCNGG | 2 cut(s) | 1111, 1511 |
| Mva1269I | GAATGC | 1 cut(s) | 932 |
| MvaI | CCWGG | 2 cut(s) | 1111, 1511 |
| MvnI | CGCG | 2 cut(s) | 3074, 3080 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 348, 3209, 3413 |
| NaeI | GCCGGC | 1 cut(s) | 214 |
| NdeII | GATC | 7 cut(s) | 204, 764, 777, 874, 916, 3424, 3472 |
| NgoMIV | GCCGGC | 1 cut(s) | 212 |
| NheI | GCTAGC | 2 cut(s) | 3208, 3230 |
| NlaIV | GGNNCC | 1 cut(s) | 217 |
| NmeAIII | GCCGAG | 2 cut(s) | 3217, 3442 |
| NmuCI | GTSAC | 1 cut(s) | 2777 |
| NsiI | ATGCAT | 2 cut(s) | 1251, 2603 |
| NspI | RCATGY | 2 cut(s) | 2013, 2349 |
| NspV | TTCGAA | 2 cut(s) | 503, 2926 |
| PagI | TCATGA | 1 cut(s) | 2604 |
| PctI | GAATGC | 1 cut(s) | 932 |
| PdiI | GCCGGC | 1 cut(s) | 214 |
| PdmI | GAANNNNTTC | 2 cut(s) | 8, 537 |
| Pfl23II | CGTACG | 1 cut(s) | 3075 |
| PflFI | GACNNNGTC | 1 cut(s) | 1025 |
| PinAI | ACCGGT | 1 cut(s) | 3272 |
| PkrI | GCNGC | 3 cut(s) | 676, 1534, 3319 |
| Ple19I | CGATCG | 1 cut(s) | 767 |
| PmaCI | CACGTG | 1 cut(s) | 2744 |
| PmlI | CACGTG | 1 cut(s) | 2744 |
| Ppu21I | YACGTR | 1 cut(s) | 2744 |
| PshBI | ATTAAT | 1 cut(s) | 2027 |
| Psp6I | CCWGG | 2 cut(s) | 1109, 1509 |
| PspCI | CACGTG | 1 cut(s) | 2744 |
| PspEI | GGTNACC | 1 cut(s) | 3275 |
| PspGI | CCWGG | 2 cut(s) | 1109, 1509 |
| PspLI | CGTACG | 1 cut(s) | 3075 |
| PspN4I | GGNNCC | 1 cut(s) | 217 |
| PsyI | GACNNNGTC | 1 cut(s) | 1025 |
| PvuI | CGATCG | 1 cut(s) | 767 |
| RsaI | GTAC | 7 cut(s) | 419, 2149, 2309, 2463, 2478, 2676, 3077 |
| RsaNI | GTAC | 7 cut(s) | 418, 2148, 2308, 2462, 2477, 2675, 3076 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1014, 2852 |
| SatI | GCNGC | 3 cut(s) | 675, 1533, 3318 |
| Sau3AI | GATC | 7 cut(s) | 204, 764, 777, 874, 916, 3424, 3472 |
| ScrFI | CCNGG | 2 cut(s) | 1111, 1511 |
| SduI | GDGCHC | 4 cut(s) | 1890, 2107, 2743, 3205 |
| SfaNI | GCATC | 5 cut(s) | 123, 1110, 1377, 2610, 3181 |
| SfcI | CTRYAG | 1 cut(s) | 679 |
| SfuI | TTCGAA | 2 cut(s) | 503, 2926 |
| SmiI | ATTTAAAT | 1 cut(s) | 2214 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1014, 2852 |
| SmlI | CTYRAG | 2 cut(s) | 452, 2155 |
| SmoI | CTYRAG | 2 cut(s) | 452, 2155 |
| SsiI | CCGC | 5 cut(s) | 122, 1476, 2349, 3080, 3318 |
| SspI | AATATT | 4 cut(s) | 1679, 2191, 2683, 2794 |
| SspMI | CTAG | 8 cut(s) | 366, 2015, 2327, 2426, 2430, 3209, 3222, 3231 |
| StyD4I | CCNGG | 2 cut(s) | 1109, 1509 |
| StyI | CCWWGG | 2 cut(s) | 3240, 3480 |
| SwaI | ATTTAAAT | 1 cut(s) | 2214 |
| TaaI | ACNGT | 4 cut(s) | 318, 440, 1636, 3292 |
| TaiI | ACGT | 5 cut(s) | 163, 186, 1938, 2242, 2746 |
| TaqI | TCGA | 6 cut(s) | 374, 503, 763, 958, 2926, 3294 |
| TaqII | GACCGA | 1 cut(s) | 1271 |
| TatI | WGTACW | 3 cut(s) | 417, 2307, 2674 |
| TauI | GCSGC | 1 cut(s) | 3320 |
| TscAI | CASTG | 4 cut(s) | 2367, 3210, 3256, 3264 |
| TseFI | GTSAC | 1 cut(s) | 2777 |
| TseI | GCWGC | 2 cut(s) | 674, 1532 |
| Tsp45I | GTSAC | 1 cut(s) | 2777 |
| TspGWI | ACGGA | 1 cut(s) | 2903 |
| TspRI | CASTG | 4 cut(s) | 2367, 3210, 3256, 3264 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1025 |
| Vha464I | CTTAAG | 1 cut(s) | 452 |
| VneI | GTGCAC | 1 cut(s) | 3201 |
| VspI | ATTAAT | 1 cut(s) | 2027 |
| XbaI | TCTAGA | 1 cut(s) | 3221 |
| XceI | RCATGY | 2 cut(s) | 2013, 2349 |
| XmiI | GTMKAC | 2 cut(s) | 1020, 2642 |
| XmnI | GAANNNNTTC | 2 cut(s) | 8, 537 |
| XspI | CTAG | 8 cut(s) | 366, 2015, 2327, 2426, 2430, 3209, 3222, 3231 |
| Zsp2I | ATGCAT | 2 cut(s) | 1251, 2603 |