pycom13g27470

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0024246
Physical Location & Seq
Forward (+)
23716067 .. 23718991
2925 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g27470.1

Sequence Viewer

Length: 1461 bp
ATGTTTCTTTTACAAATAAAGTATATTTACAGTTTTGGTCATTTTATACAGTCACAGCTGTTTTACGTAAAAGTTTACCAAACATTATCATCTTGCTTTTTTTTCCAAAAGCACTTTCACAAAAAAGTTTACCAAACACTCTGCTGCTTTTTTTCACAGTACAACAGAAGCAGTTTTTTTTCAGAACACAACAATACCAAACCAACCCTTACTCTCATGTTGATCCAGTCGGCTTTTCACCGTCTTTTTCTTAGGCTACCATGGAGAACCTCAGTTTATTTGGAAAAAGGAAAGTCACCCAATATATCGATCTTATCGGAAAATCTTTCCCAATGTCTGAAGATCTGTTTTAGCCCACGACGACGTTTTGTCATCGTAGTGAGCATGGTGCACCGATTGGACAATGACGTTTCTTTTCCTCATCAAATCAATTCTTTTACGGACCACATACAAATCAATTTTTTTGTGGGTGTGAATTTAATGTCTTTTTTTTTTTTTTTTGGTTTTGGAAAAACTAAAACATGTAAGAAATCAATCCATAATTTCGAGACTTTTCCATCTGTGTCTCTCCGGCAAAATAAGTTGGAGTTGGGTTCTGGGAGAAAGGAGTGGGAAGAGGAGAATAATGAGTTATCAAATCAATCAGAAGACAGAAAGTTAAATTTGAAAGAACCAAATTTACAGTTGTCAATTACGTATTGGTTAAGAACTCCATGTCAGCTTAAGTGCCGGTCACCTGGAAAAATTTCTCCACCATTTGGGCCCCCACTCCGGCTCGTGAACCTCGGCAAGTGGATCGTCCGCGTCGCCGATTCGACTCGGTCCCTCCATCTCGATCTCTCCGAGCGCGACTCGCTCCATTCGCAAGCTCTCTCCTCCGGCCGCGCTTCCTTGCACGGGCGTCGATGTTCGCGACACGTCTCAAATCATCACTGCTTGACAATTGAAGGTTCATCTGTAGTGTTCTGTTTGGATGGGGCTGATTTACCCAAAGATGATTTCTACCAGTGCTATATGATTGTTGTTCAAGGTGCAAAAAATGTCATCAGTGCTTGTCCGGTGTGCAAAGTTAGGCAGTTAATATACAATAGTTCTGCTGGTGTAGTTTTTGATGGTTCAAAAGATATATTAAATGGAGATGAATCTTTGGCATACCCTTGGAAATTTGATGATATGTTGAGTGATCTCAAGGCTCAAGCAGAATCACTTGTCCTGTTGTCTAATGACATTGATGGTCTTTTAACATGTGCACTTAGTCCTAGCAATGCATTTGGACCTGGAGACACGTATCTTGTGCCATTTTTAGTGAACTTGGCAAAATCTGAAGAAGCCTTAGAATTTCGGATGGTCTCTGCTGCTGGAAAGGGACTTACAATTTTATGTCTCCTTGGTGCACATCACTTTATTTTTCTGTTTCTTCTGGGTCCACAGTTAAGGAGGTTACTTGTGATCAGTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01130 map00100 map01100 map01130
Pfam Domains
Protein Families
3Beta_HSD Reticulon

Protein Analysis

487

Amino Acids

55.41

Weight (kDa)

9.07

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 259 - 445 3.8e-33 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024246)

Species Orthologous Gene IDs
malus_domestica MD13G1270400.v1.1
pyrus_communis pycom13g27470 pycom849g00020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 758
AccII CGCG 4 cut(s) 804, 849, 885, 913
AciI CCGC 2 cut(s) 802, 883
AclWI GGATC 2 cut(s) 217, 803
AcoI YGGCCR 1 cut(s) 880
AcsI RAATTY 6 cut(s) 475, 661, 676, 744, 1163, 1337
AcuI CTGAAG 2 cut(s) 359, 1344
AcyI GRCGYC 1 cut(s) 901
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 3 cut(s) 758, 771, 897
AflII CTTAAG 1 cut(s) 722
AflIII ACRYGT 4 cut(s) 521, 916, 1244, 1284
AgsI TTSAA 4 cut(s) 667, 947, 1028, 1119
AhdI GACNNNNNGTC 1 cut(s) 368
AjiI CACGTC 1 cut(s) 919
AjnI CCWGG 2 cut(s) 736, 1276
AjuI GAANNNNNNNTTGG 4 cut(s) 99, 131, 983, 1015
AluBI AGCT 3 cut(s) 58, 721, 869
AluI AGCT 3 cut(s) 58, 721, 869
Alw21I GWGCWC 3 cut(s) 393, 1252, 1396
Alw26I GTCTC 6 cut(s) 542, 570, 925, 1275, 1354, 1388
Alw44I GTGCAC 3 cut(s) 389, 1248, 1392
AlwI GGATC 2 cut(s) 217, 803
AoxI GGCC 2 cut(s) 761, 880
ApaI GGGCCC 1 cut(s) 765
ApaLI GTGCAC 3 cut(s) 389, 1248, 1392
ApeKI GCWGC 2 cut(s) 144, 1355
ApoI RAATTY 6 cut(s) 475, 661, 676, 744, 1163, 1337
Asp700I GAANNNNTTC 1 cut(s) 745
AspLEI GCGC 2 cut(s) 849, 887
AspS9I GGNCC 6 cut(s) 442, 761, 762, 822, 1274, 1424
AsuHPI GGTGA 3 cut(s) 230, 288, 726
AvaII GGWCC 4 cut(s) 442, 822, 1274, 1424
BaeGI GKGCMC 4 cut(s) 393, 765, 1252, 1396
BanII GRGCYC 1 cut(s) 765
BauI CACGAG 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 3 cut(s) 393, 1252, 1396
BbvI GCAGC 2 cut(s) 131, 1342
BccI CCATC 6 cut(s) 565, 837, 968, 1106, 1226, 1339
BcgI CGANNNNNNTGC 4 cut(s) 778, 812, 884, 918
BciT130I CCWGG 2 cut(s) 738, 1278
BclI TGATCA 1 cut(s) 1449
BcoDI GTCTC 6 cut(s) 542, 570, 925, 1275, 1354, 1388
BfaI CTAG 1 cut(s) 1260
BfmI CTRYAG 2 cut(s) 957, 1457
BfrI CTTAAG 1 cut(s) 722
BglII AGATCT 1 cut(s) 342
BisI GCNGC 3 cut(s) 145, 883, 1356
BlsI GCNGC 3 cut(s) 146, 884, 1357
Bme1390I CCNGG 2 cut(s) 738, 1278
Bme18I GGWCC 4 cut(s) 442, 822, 1274, 1424
BmeRI GACNNNNNGTC 1 cut(s) 368
BmgBI CACGTC 1 cut(s) 919
BmgT120I GGNCC 6 cut(s) 442, 761, 762, 822, 1274, 1424
BmiI GGNNCC 4 cut(s) 763, 764, 824, 1425
BmrFI CCNGG 2 cut(s) 738, 1278
BpiI GAAGAC 1 cut(s) 654
BplI GAGNNNNNCTC 2 cut(s) 836, 868
BpmI CTGGAG 1 cut(s) 1299
BpuEI CTTGAG 2 cut(s) 1172, 1179
Bsa29I ATCGAT 1 cut(s) 308
BsaAI YACGTR 3 cut(s) 67, 696, 1287
BsaHI GRCGYC 1 cut(s) 901
BsaI GGTCTC 1 cut(s) 1354
BsaJI CCNNGG 4 cut(s) 260, 784, 1157, 1387
BsaWI WCCGGW 1 cut(s) 1057
Bsc4I CCNNNNNNNGG 3 cut(s) 758, 771, 897
Bse118I RCCGGY 1 cut(s) 729
Bse1I ACTGG 2 cut(s) 226, 1006
Bse3DI GCAATG 1 cut(s) 1270
BseBI CCWGG 2 cut(s) 738, 1278
BseCI ATCGAT 1 cut(s) 308
BseDI CCNNGG 4 cut(s) 260, 784, 1157, 1387
BseGI GGATG 2 cut(s) 979, 1350
BseLI CCNNNNNNNGG 3 cut(s) 758, 771, 897
BseMI GCAATG 1 cut(s) 1270
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 2 cut(s) 226, 1006
BseRI GAGGAG 2 cut(s) 632, 865
BseSI GKGCMC 4 cut(s) 393, 765, 1252, 1396
BseX3I CGGCCG 1 cut(s) 880
BseXI GCAGC 2 cut(s) 131, 1342
Bsh1236I CGCG 4 cut(s) 804, 849, 885, 913
Bsh1285I CGRYCG 1 cut(s) 883
BshFI GGCC 2 cut(s) 763, 882
BshVI ATCGAT 1 cut(s) 308
BsiEI CGRYCG 1 cut(s) 883
BsiHKAI GWGCWC 3 cut(s) 393, 1252, 1396
BsiSI CCGG 5 cut(s) 571, 730, 772, 879, 1058
BslFI GGGAC 2 cut(s) 808, 1380
BslI CCNNNNNNNGG 3 cut(s) 758, 771, 897
BsmAI GTCTC 6 cut(s) 542, 570, 925, 1275, 1354, 1388
BsmBI CGTCTC 1 cut(s) 925
BsmFI GGGAC 2 cut(s) 808, 1380
BsnI GGCC 2 cut(s) 763, 882
Bso31I GGTCTC 1 cut(s) 1354
Bsp120I GGGCCC 1 cut(s) 761
Bsp1286I GDGCHC 4 cut(s) 393, 765, 1252, 1396
Bsp143I GATC 7 cut(s) 222, 309, 342, 795, 835, 1183, 1449
Bsp19I CCATGG 1 cut(s) 260
Bsp68I TCGCGA 1 cut(s) 913
BspACI CCGC 2 cut(s) 802, 883
BspANI GGCC 2 cut(s) 763, 882
BspCNI CTCAG 1 cut(s) 284
BspDI ATCGAT 1 cut(s) 308
BspFNI CGCG 4 cut(s) 804, 849, 885, 913
BspLI GGNNCC 4 cut(s) 763, 764, 824, 1425
BspPI GGATC 2 cut(s) 217, 803
BspTI CTTAAG 1 cut(s) 722
BspTNI GGTCTC 1 cut(s) 1354
BsrDI GCAATG 1 cut(s) 1270
BsrFI RCCGGY 1 cut(s) 729
BsrI ACTGG 2 cut(s) 226, 1006
BssAI RCCGGY 1 cut(s) 729
BssECI CCNNGG 4 cut(s) 260, 784, 1157, 1387
BssMI GATC 7 cut(s) 222, 309, 342, 795, 835, 1183, 1449
BssNI GRCGYC 1 cut(s) 901
BssSI CACGAG 1 cut(s) 776
BssT1I CCWWGG 3 cut(s) 260, 1157, 1387
Bst2BI CACGAG 1 cut(s) 776
Bst2UI CCWGG 2 cut(s) 738, 1278
Bst4CI ACNGT 6 cut(s) 32, 51, 159, 242, 684, 1431
Bst6I CTCTTC 1 cut(s) 609
BstACI GRCGYC 1 cut(s) 901
BstAFI CTTAAG 1 cut(s) 722
BstBAI YACGTR 3 cut(s) 67, 696, 1287
BstC8I GCNNGC 1 cut(s) 867
BstDEI CTNAG 4 cut(s) 251, 271, 1253, 1333
BstDSI CCRYGG 1 cut(s) 260
BstEII GGTNACC 1 cut(s) 732
BstF5I GGATG 2 cut(s) 979, 1350
BstFNI CGCG 4 cut(s) 804, 849, 885, 913
BstHHI GCGC 2 cut(s) 849, 887
BstKTI GATC 7 cut(s) 225, 312, 345, 798, 838, 1186, 1452
BstMAI GTCTC 6 cut(s) 542, 570, 925, 1275, 1354, 1388
BstMBI GATC 7 cut(s) 222, 309, 342, 795, 835, 1183, 1449
BstMCI CGRYCG 1 cut(s) 883
BstMWI GCNNNNNNNGC 2 cut(s) 853, 862
BstNI CCWGG 2 cut(s) 738, 1278
BstNSI RCATGY 2 cut(s) 525, 1248
BstPI GGTNACC 1 cut(s) 732
BstSCI CCNGG 2 cut(s) 736, 1276
BstSFI CTRYAG 2 cut(s) 957, 1457
BstSLI GKGCMC 4 cut(s) 393, 765, 1252, 1396
BstSNI TACGTA 2 cut(s) 67, 696
BstUI CGCG 4 cut(s) 804, 849, 885, 913
BstV1I GCAGC 2 cut(s) 131, 1342
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
BstZI CGGCCG 1 cut(s) 880
Bsu15I ATCGAT 1 cut(s) 308
BsuRI GGCC 2 cut(s) 763, 882
BsuTUI ATCGAT 1 cut(s) 308
BtgI CCRYGG 1 cut(s) 260
BtrI CACGTC 1 cut(s) 919
BtsCI GGATG 2 cut(s) 979, 1350
BtsI GCAGTG 1 cut(s) 931
BtsIMutI CAGTG 3 cut(s) 931, 1013, 1054
BtuMI TCGCGA 1 cut(s) 913
Cac8I GCNNGC 1 cut(s) 867
CfoI GCGC 2 cut(s) 849, 887
Cfr10I RCCGGY 1 cut(s) 729
Cfr13I GGNCC 6 cut(s) 442, 761, 762, 822, 1274, 1424
ClaI ATCGAT 1 cut(s) 308
CseI GACGC 2 cut(s) 793, 890
Csp6I GTAC 1 cut(s) 160
CviAII CATG 6 cut(s) 217, 261, 385, 522, 714, 1245
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 4 cut(s) 251, 271, 1253, 1333
DpnI GATC 7 cut(s) 224, 311, 344, 797, 837, 1185, 1451
DpnII GATC 7 cut(s) 222, 309, 342, 795, 835, 1183, 1449
DriI GACNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 880
EagI CGGCCG 1 cut(s) 880
Eam1104I CTCTTC 1 cut(s) 609
Eam1105I GACNNNNNGTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 609
EclXI CGGCCG 1 cut(s) 880
Eco105I TACGTA 2 cut(s) 67, 696
Eco130I CCWWGG 3 cut(s) 260, 1157, 1387
Eco24I GRGCYC 1 cut(s) 765
Eco31I GGTCTC 1 cut(s) 1354
Eco47I GGWCC 4 cut(s) 442, 822, 1274, 1424
Eco52I CGGCCG 1 cut(s) 880
Eco57I CTGAAG 2 cut(s) 359, 1344
Eco91I GGTNACC 1 cut(s) 732
EcoO109I RGGNCCY 1 cut(s) 762
EcoO65I GGTNACC 1 cut(s) 732
EcoRII CCWGG 2 cut(s) 736, 1276
EcoT14I CCWWGG 3 cut(s) 260, 1157, 1387
EcoT22I ATGCAT 1 cut(s) 1270
EcoT38I GRGCYC 1 cut(s) 765
ErhI CCWWGG 3 cut(s) 260, 1157, 1387
Esp3I CGTCTC 1 cut(s) 925
FaeI CATG 6 cut(s) 220, 264, 388, 525, 717, 1248
FaqI GGGAC 2 cut(s) 808, 1380
FatI CATG 6 cut(s) 216, 260, 384, 521, 713, 1244
FbaI TGATCA 1 cut(s) 1449
Fnu4HI GCNGC 3 cut(s) 145, 883, 1356
FokI GGATG 2 cut(s) 986, 1357
FriOI GRGCYC 1 cut(s) 765
Fsp4HI GCNGC 3 cut(s) 145, 883, 1356
FspBI CTAG 1 cut(s) 1260
GlaI GCGC 2 cut(s) 848, 886
GluI GCNGC 3 cut(s) 145, 883, 1356
GsuI CTGGAG 1 cut(s) 1299
HaeIII GGCC 2 cut(s) 763, 882
HapII CCGG 5 cut(s) 571, 730, 772, 879, 1058
HgaI GACGC 2 cut(s) 793, 890
HhaI GCGC 2 cut(s) 849, 887
Hin1I GRCGYC 1 cut(s) 901
Hin1II CATG 6 cut(s) 220, 264, 388, 525, 717, 1248
Hin6I GCGC 2 cut(s) 847, 885
HinP1I GCGC 2 cut(s) 847, 885
HinfI GANTC 5 cut(s) 812, 817, 851, 1142, 1202
HpaII CCGG 5 cut(s) 571, 730, 772, 879, 1058
HphI GGTGA 3 cut(s) 230, 288, 726
Hpy166II GTNNAC 8 cut(s) 76, 130, 391, 781, 1250, 1309, 1394, 1427
Hpy188I TCNGA 7 cut(s) 184, 319, 339, 646, 844, 1324, 1344
Hpy188III TCNNGA 4 cut(s) 547, 778, 833, 912
Hpy8I GTNNAC 8 cut(s) 76, 130, 391, 781, 1250, 1309, 1394, 1427
Hpy99I CGWCG 4 cut(s) 363, 366, 809, 906
HpyAV CCTTC 1 cut(s) 941
HpyCH4III ACNGT 6 cut(s) 32, 51, 159, 242, 684, 1431
HpyCH4IV ACGT 6 cut(s) 66, 364, 408, 695, 918, 1286
HpyCH4V TGCA 7 cut(s) 391, 895, 1034, 1065, 1250, 1268, 1394
HpyF10VI GCNNNNNNNGC 2 cut(s) 853, 862
HpyF3I CTNAG 4 cut(s) 251, 271, 1253, 1333
HpySE526I ACGT 6 cut(s) 66, 364, 408, 695, 918, 1286
Hsp92I GRCGYC 1 cut(s) 901
Hsp92II CATG 6 cut(s) 220, 264, 388, 525, 717, 1248
HspAI GCGC 2 cut(s) 847, 885
Ksp22I TGATCA 1 cut(s) 1449
Kzo9I GATC 7 cut(s) 222, 309, 342, 795, 835, 1183, 1449
LmnI GCTCC 1 cut(s) 861
Lsp1109I GCAGC 2 cut(s) 131, 1342
MaeI CTAG 1 cut(s) 1260
MaeII ACGT 6 cut(s) 66, 364, 408, 695, 918, 1286
MaeIII GTNAC 4 cut(s) 51, 294, 732, 1440
MalI GATC 7 cut(s) 224, 311, 344, 797, 837, 1185, 1451
MboI GATC 7 cut(s) 222, 309, 342, 795, 835, 1183, 1449
MboII GAAGA 5 cut(s) 352, 626, 659, 1337, 1409
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 1 cut(s) 342
MhlI GDGCHC 4 cut(s) 393, 765, 1252, 1396
MlyI GAGTC 2 cut(s) 811, 845
MmeI TCCRAC 1 cut(s) 564
MnlI CCTC 7 cut(s) 280, 429, 610, 794, 836, 886, 1431
Mph1103I ATGCAT 1 cut(s) 1270
MroXI GAANNNNTTC 1 cut(s) 745
MseI TTAA 8 cut(s) 479, 659, 704, 723, 1079, 1130, 1241, 1433
MslI CAYNNNNRTG 1 cut(s) 377
MspA1I CMGCKG 1 cut(s) 58
MspCI CTTAAG 1 cut(s) 722
MspI CCGG 5 cut(s) 571, 730, 772, 879, 1058
MspR9I CCNGG 2 cut(s) 738, 1278
MunI CAATTG 1 cut(s) 942
MvaI CCWGG 2 cut(s) 738, 1278
MvnI CGCG 4 cut(s) 804, 849, 885, 913
MwoI GCNNNNNNNGC 2 cut(s) 853, 862
NcoI CCATGG 1 cut(s) 260
NdeII GATC 7 cut(s) 222, 309, 342, 795, 835, 1183, 1449
NlaIII CATG 6 cut(s) 220, 264, 388, 525, 717, 1248
NlaIV GGNNCC 4 cut(s) 763, 764, 824, 1425
NmeAIII GCCGAG 1 cut(s) 765
NmuCI GTSAC 3 cut(s) 51, 294, 732
NruI TCGCGA 1 cut(s) 913
NsiI ATGCAT 1 cut(s) 1270
NspI RCATGY 2 cut(s) 525, 1248
PciI ACATGT 2 cut(s) 521, 1244
PcsI WCGNNNNNNNCGW 2 cut(s) 840, 910
PdmI GAANNNNTTC 1 cut(s) 745
PfeI GAWTC 3 cut(s) 812, 1142, 1202
PflFI GACNNNGTC 1 cut(s) 820
PflMI CCANNNNNTGG 1 cut(s) 758
PkrI GCNGC 3 cut(s) 146, 884, 1357
PleI GAGTC 2 cut(s) 811, 845
PpsI GAGTC 2 cut(s) 811, 845
Ppu21I YACGTR 3 cut(s) 67, 696, 1287
PscI ACATGT 2 cut(s) 521, 1244
Psp6I CCWGG 2 cut(s) 736, 1276
PspEI GGTNACC 1 cut(s) 732
PspGI CCWGG 2 cut(s) 736, 1276
PspN4I GGNNCC 4 cut(s) 763, 764, 824, 1425
PspOMI GGGCCC 1 cut(s) 761
PspPI GGNCC 6 cut(s) 442, 761, 762, 822, 1274, 1424
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
PsuI RGATCY 1 cut(s) 342
PsyI GACNNNGTC 1 cut(s) 820
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 913
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
RseI CAYNNNNRTG 1 cut(s) 377
SaqAI TTAA 8 cut(s) 479, 659, 704, 723, 1079, 1130, 1241, 1433
SatI GCNGC 3 cut(s) 145, 883, 1356
Sau3AI GATC 7 cut(s) 222, 309, 342, 795, 835, 1183, 1449
Sau96I GGNCC 6 cut(s) 442, 761, 762, 822, 1274, 1424
SchI GAGTC 2 cut(s) 811, 845
ScrFI CCNGG 2 cut(s) 738, 1278
SduI GDGCHC 4 cut(s) 393, 765, 1252, 1396
SfcI CTRYAG 2 cut(s) 957, 1457
SinI GGWCC 4 cut(s) 442, 822, 1274, 1424
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 3 cut(s) 722, 1187, 1194
SmoI CTYRAG 3 cut(s) 722, 1187, 1194
SnaBI TACGTA 2 cut(s) 67, 696
SsiI CCGC 2 cut(s) 802, 883
SspMI CTAG 1 cut(s) 1260
StyD4I CCNGG 2 cut(s) 736, 1276
StyI CCWWGG 3 cut(s) 260, 1157, 1387
TaaI ACNGT 6 cut(s) 32, 51, 159, 242, 684, 1431
TaiI ACGT 6 cut(s) 69, 367, 411, 698, 921, 1289
TaqI TCGA 5 cut(s) 308, 546, 815, 834, 904
TaqII GACCGA 1 cut(s) 810
TatI WGTACW 1 cut(s) 159
TauI GCSGC 1 cut(s) 885
TfiI GAWTC 3 cut(s) 812, 1142, 1202
Tru1I TTAA 8 cut(s) 479, 659, 704, 723, 1079, 1130, 1241, 1433
Tru9I TTAA 8 cut(s) 479, 659, 704, 723, 1079, 1130, 1241, 1433
TscAI CASTG 3 cut(s) 938, 1013, 1054
TseFI GTSAC 3 cut(s) 51, 294, 732
TseI GCWGC 2 cut(s) 144, 1355
Tsp45I GTSAC 3 cut(s) 51, 294, 732
TspDTI ATGAA 2 cut(s) 942, 1155
TspGWI ACGGA 1 cut(s) 455
TspRI CASTG 3 cut(s) 938, 1013, 1054
Tth111I GACNNNGTC 1 cut(s) 820
Van91I CCANNNNNTGG 1 cut(s) 758
Vha464I CTTAAG 1 cut(s) 722
VneI GTGCAC 3 cut(s) 389, 1248, 1392
VpaK11BI GGWCC 4 cut(s) 442, 822, 1274, 1424
XapI RAATTY 6 cut(s) 475, 661, 676, 744, 1163, 1337
XceI RCATGY 2 cut(s) 525, 1248
XmnI GAANNNNTTC 1 cut(s) 745
XspI CTAG 1 cut(s) 1260
Zsp2I ATGCAT 1 cut(s) 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.