pycom14g06660

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0029456
Physical Location & Seq
Forward (+)
6667867 .. 6668936
1070 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06660.2

Sequence Viewer

Length: 624 bp
ATGGCGGAGTACGACTTAACTCCACGAATGGCGCCCAACCTAGACCGGCATCTGGTTTTTCCCCTCCTCGAATTTCTGCAGGAGCGGCAGATGTACCCAGATGAGCAGATCCTCAAGTCCAAGATCGAGCTCCTCAACAAGACCAACATGGTCGATTACGCCATGGACATCCACAAGTCCCTCTATCACACCGAAGATGTGCCCCAAGATATGGTGGATAGGAGGGTAGAGGTGGTGGCTCGCCTCAAGGCTCTGGAGGAGTCGGCAGCGCCCCTTGTCAATTTCTTGCAGAACGCCGCGGCGTTTCAGGAGCTGAAGGCTGACAAGCAGTACAATCTACAGATGCTCAATGACCGATACCAGATTGGTCCAGAGCAGATCGAGGCGTTGTATCAGTATGCAAAATTTCAGTTTGAATGTGGTAACTACTCTGGTGCTGCTGATTATCTCTATCAGTACAGGGCTTTGTGCACAAACAGTGACAGGAATCTGAGTGCATTGTGGGGAAAGCTGGCGGCTGAGATATTGATGCAGAACTGGGATATTGCCCTTGAAGAGCTTAACCGCTTGAAAGAAATTATTGATTCAAAGTTTCAGTTCACCTATGAATCAAGTGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005852 GO:0005886 GO:0006139 GO:0006351 GO:0006352 GO:0006412 GO:0006413 GO:0006417 GO:0006518 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009651 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016070 GO:0017148 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0022414 GO:0030371 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031597 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034645 GO:0034654 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0045182 GO:0046483 GO:0048367 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0080090 GO:0090304 GO:0090567 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1905368 GO:1905369 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko03013 ko05160 map03013 map05160
Pfam Domains
Protein Families
PCI eIF3_N

Protein Analysis

208

Amino Acids

24.24

Weight (kDa)

4.84

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029456)

Species Orthologous Gene IDs
pyrus_communis pycom14g06660
rosa_multiflora Rmu_sc0020222.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 211
AccBSI CCGCTC 1 cut(s) 85
AccII CGCG 1 cut(s) 299
AciI CCGC 6 cut(s) 5, 85, 297, 299, 515, 565
AclWI GGATC 1 cut(s) 103
AcsI RAATTY 2 cut(s) 71, 404
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 4 cut(s) 11, 95, 332, 458
AfiI CCNNNNNNNGG 2 cut(s) 52, 211
AgsI TTSAA 4 cut(s) 416, 554, 571, 588
AluBI AGCT 4 cut(s) 130, 313, 511, 559
AluI AGCT 4 cut(s) 130, 313, 511, 559
Alw21I GWGCWC 2 cut(s) 132, 473
Alw44I GTGCAC 1 cut(s) 469
AlwI GGATC 1 cut(s) 103
AlwNI CAGNNNCTG 1 cut(s) 313
ApaLI GTGCAC 1 cut(s) 469
ApeKI GCWGC 2 cut(s) 266, 437
ApoI RAATTY 2 cut(s) 71, 404
AspLEI GCGC 2 cut(s) 34, 271
AspS9I GGNCC 1 cut(s) 368
AsuHPI GGTGA 1 cut(s) 592
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 2 cut(s) 204, 473
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 132
Bbv12I GWGCWC 2 cut(s) 132, 473
BbvI GCAGC 2 cut(s) 278, 424
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 2 cut(s) 77, 338
BfoI RGCGCY 2 cut(s) 35, 272
BisI GCNGC 6 cut(s) 86, 267, 297, 300, 438, 516
BlsI GCNGC 6 cut(s) 87, 268, 298, 301, 439, 517
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 1 cut(s) 33
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 3 cut(s) 58, 333, 519
BmuI ACTGGG 1 cut(s) 547
BpmI CTGGAG 1 cut(s) 275
BpuEI CTTGAG 2 cut(s) 98, 230
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 162, 297
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 211
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 1 cut(s) 542
BseDI CCNNGG 2 cut(s) 162, 297
BseGI GGATG 1 cut(s) 168
BseLI CCNNNNNNNGG 2 cut(s) 52, 211
BseMII CTCAG 2 cut(s) 482, 510
BseNI ACTGG 1 cut(s) 542
BseRI GAGGAG 3 cut(s) 56, 122, 272
BseSI GKGCMC 2 cut(s) 204, 473
BseXI GCAGC 2 cut(s) 278, 424
Bsh1236I CGCG 1 cut(s) 299
BshNI GGYRCC 1 cut(s) 31
BsiHKAI GWGCWC 2 cut(s) 132, 473
BsiSI CCGG 1 cut(s) 46
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 2 cut(s) 52, 211
BsmFI GGGAC 1 cut(s) 163
Bsp1286I GDGCHC 3 cut(s) 132, 204, 473
Bsp143I GATC 3 cut(s) 108, 123, 378
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 6 cut(s) 5, 85, 297, 299, 515, 565
BspCNI CTCAG 2 cut(s) 483, 511
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 1 cut(s) 33
BspMAI CTGCAG 1 cut(s) 81
BspPI GGATC 1 cut(s) 103
BspQI GCTCTTC 1 cut(s) 549
BspT107I GGYRCC 1 cut(s) 31
BsrBI CCGCTC 1 cut(s) 85
BsrFI RCCGGY 1 cut(s) 45
BsrI ACTGG 1 cut(s) 542
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 162, 297
BssMI GATC 3 cut(s) 108, 123, 378
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 162
Bst4CI ACNGT 1 cut(s) 479
Bst6I CTCTTC 1 cut(s) 549
BstACI GRCGYC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 241, 513
BstDEI CTNAG 2 cut(s) 491, 519
BstDSI CCRYGG 2 cut(s) 162, 297
BstF5I GGATG 1 cut(s) 168
BstFNI CGCG 1 cut(s) 299
BstH2I RGCGCY 2 cut(s) 35, 272
BstHHI GCGC 2 cut(s) 34, 271
BstKTI GATC 3 cut(s) 111, 126, 381
BstMBI GATC 3 cut(s) 108, 123, 378
BstMWI GCNNNNNNNGC 1 cut(s) 85
BstSFI CTRYAG 2 cut(s) 77, 338
BstSLI GKGCMC 2 cut(s) 204, 473
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 2 cut(s) 278, 424
BstX2I RGATCY 1 cut(s) 108
BstYI RGATCY 1 cut(s) 108
BtgI CCRYGG 2 cut(s) 162, 297
BtsCI GGATG 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 484
Cac8I GCNNGC 2 cut(s) 241, 513
CaiI CAGNNNCTG 1 cut(s) 313
CfoI GCGC 2 cut(s) 34, 271
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 368
Cfr42I CCGCGG 1 cut(s) 300
Csp6I GTAC 4 cut(s) 10, 94, 331, 457
CviAII CATG 2 cut(s) 148, 163
CviJI RGCY 9 cut(s) 130, 239, 251, 313, 320, 464, 511, 518, 559
CviKI_1 RGCY 9 cut(s) 130, 239, 251, 313, 320, 464, 511, 518, 559
CviQI GTAC 4 cut(s) 10, 94, 331, 457
DdeI CTNAG 2 cut(s) 491, 519
DinI GGCGCC 1 cut(s) 33
DpnI GATC 3 cut(s) 110, 125, 380
DpnII GATC 3 cut(s) 108, 123, 378
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 368
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 132
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 2 cut(s) 151, 166
FaiI YATR 5 cut(s) 149, 164, 212, 399, 606
FaqI GGGAC 1 cut(s) 163
FatI CATG 2 cut(s) 147, 162
Fnu4HI GCNGC 6 cut(s) 86, 267, 297, 300, 438, 516
FokI GGATG 1 cut(s) 155
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 6 cut(s) 86, 267, 297, 300, 438, 516
FspBI CTAG 1 cut(s) 41
GlaI GCGC 2 cut(s) 33, 270
GluI GCNGC 6 cut(s) 86, 267, 297, 300, 438, 516
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 2 cut(s) 35, 272
HapII CCGG 1 cut(s) 46
HhaI GCGC 2 cut(s) 34, 271
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 2 cut(s) 151, 166
Hin6I GCGC 2 cut(s) 32, 269
HinP1I GCGC 2 cut(s) 32, 269
HinfI GANTC 4 cut(s) 260, 487, 584, 608
HpaII CCGG 1 cut(s) 46
HphI GGTGA 1 cut(s) 592
Hpy166II GTNNAC 2 cut(s) 471, 600
Hpy188I TCNGA 1 cut(s) 492
Hpy188III TCNNGA 3 cut(s) 254, 308, 371
Hpy8I GTNNAC 2 cut(s) 471, 600
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 1 cut(s) 479
HpyCH4V TGCA 7 cut(s) 79, 289, 401, 471, 497, 532, 617
HpyF10VI GCNNNNNNNGC 1 cut(s) 85
HpyF3I CTNAG 2 cut(s) 491, 519
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 2 cut(s) 151, 166
HspAI GCGC 2 cut(s) 32, 269
KasI GGCGCC 1 cut(s) 31
KspI CCGCGG 1 cut(s) 300
Kzo9I GATC 3 cut(s) 108, 123, 378
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 3 cut(s) 82, 135, 310
Lsp1109I GCAGC 2 cut(s) 278, 424
LweI GCATC 3 cut(s) 58, 333, 519
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 2 cut(s) 422, 479
MalI GATC 3 cut(s) 110, 125, 380
MbiI CCGCTC 1 cut(s) 85
MboI GATC 3 cut(s) 108, 123, 378
MboII GAAGA 2 cut(s) 206, 566
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 3 cut(s) 132, 204, 473
MluCI AATT 4 cut(s) 71, 280, 404, 576
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 269
MseI TTAA 2 cut(s) 17, 561
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 1 cut(s) 46
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 1 cut(s) 85
NarI GGCGCC 1 cut(s) 32
NcoI CCATGG 1 cut(s) 162
NdeII GATC 3 cut(s) 108, 123, 378
NlaIII CATG 2 cut(s) 151, 166
NlaIV GGNNCC 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 479
PciSI GCTCTTC 1 cut(s) 549
PfeI GAWTC 3 cut(s) 487, 584, 608
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 6 cut(s) 87, 268, 298, 301, 439, 517
PleI GAGTC 1 cut(s) 268
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 268
Psp124BI GAGCTC 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 368
PstI CTGCAG 1 cut(s) 81
PstNI CAGNNNCTG 1 cut(s) 313
PsuI RGATCY 1 cut(s) 108
RsaI GTAC 4 cut(s) 11, 95, 332, 458
RsaNI GTAC 4 cut(s) 10, 94, 331, 457
SacI GAGCTC 1 cut(s) 132
SacII CCGCGG 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 2 cut(s) 17, 561
SatI GCNGC 6 cut(s) 86, 267, 297, 300, 438, 516
Sau3AI GATC 3 cut(s) 108, 123, 378
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 1 cut(s) 269
SduI GDGCHC 3 cut(s) 132, 204, 473
SetI ASST 7 cut(s) 42, 132, 234, 315, 513, 561, 605
SfaNI GCATC 3 cut(s) 58, 333, 519
SfcI CTRYAG 2 cut(s) 77, 338
SfoI GGCGCC 1 cut(s) 33
Sfr303I CCGCGG 1 cut(s) 300
SgrBI CCGCGG 1 cut(s) 300
SinI GGWCC 1 cut(s) 368
SmlI CTYRAG 2 cut(s) 113, 245
SmoI CTYRAG 2 cut(s) 113, 245
Sse9I AATT 4 cut(s) 71, 280, 404, 576
SsiI CCGC 6 cut(s) 5, 85, 297, 299, 515, 565
SspDI GGCGCC 1 cut(s) 31
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 132
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 1 cut(s) 479
TaqI TCGA 4 cut(s) 69, 126, 153, 381
TaqII GACCGA 1 cut(s) 369
TasI AATT 4 cut(s) 71, 280, 404, 576
TatI WGTACW 2 cut(s) 330, 456
TauI GCSGC 4 cut(s) 88, 299, 302, 518
TfiI GAWTC 3 cut(s) 487, 584, 608
Tru1I TTAA 2 cut(s) 17, 561
Tru9I TTAA 2 cut(s) 17, 561
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 2 cut(s) 266, 437
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 1 cut(s) 621
TspRI CASTG 1 cut(s) 484
Van91I CCANNNNNTGG 1 cut(s) 211
VneI GTGCAC 1 cut(s) 469
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 2 cut(s) 71, 404
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.