Rmu_sc0020222.1_g000001

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020222.1
OG0029456
Physical Location & Seq
Forward (+)
1 .. 873
873 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020222.1_g000001.1.cds

Sequence Viewer

Length: 510 bp
tggtcgactacgccatggacatccacaagtccctctatcacaccgaagatgtccccccggagatggtcgagatgagggccgaggtggtcgctcgcctcaaggctctcgaggagtccgcggcgccccttgtcaacttcttgcagaacgccgccgccgttcaggagctcaggtctgacaagcagtacaatctccagatgctcaatgaccgttaccagattggtccgcagcagatagaggcgctgtatcagtatgcaaagtttcagtttgaatgtggcaactactctggcgctgctgattatctgtatcagtaccgggcgttatgcaccaacagcgagaggagtctcagtgcattgtggggaaagctggccgctgagattttgatgcagaactgggatattgctcttgaagagcttaaccgcctgaaagaaattattgattcaaagaatttcagttcacctatgaatcaggtgcaaaacagaatatggttgatgcactggagtctcttcatat
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005852 GO:0005886 GO:0006139 GO:0006351 GO:0006352 GO:0006412 GO:0006413 GO:0006417 GO:0006518 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009651 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016070 GO:0017148 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0022414 GO:0030371 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031597 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034645 GO:0034654 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0045182 GO:0046483 GO:0048367 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0080090 GO:0090304 GO:0090567 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1905368 GO:1905369 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko03013 ko05160 map03013 map05160
Pfam Domains
Protein Families
PCI eIF3_N

Protein Analysis

169

Amino Acids

19.69

Weight (kDa)

4.95

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029456)

Species Orthologous Gene IDs
pyrus_communis pycom14g06660
rosa_multiflora Rmu_sc0020222.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 120
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 118
AciI CCGC 7 cut(s) 116, 118, 149, 152, 223, 368, 417
AcoI YGGCCR 1 cut(s) 365
AcsI RAATTY 1 cut(s) 444
AcyI GRCGYC 1 cut(s) 121
AfaI GTAC 2 cut(s) 184, 310
AfiI CCNNNNNNNGG 1 cut(s) 63
AgsI TTSAA 3 cut(s) 268, 406, 440
AluBI AGCT 3 cut(s) 165, 363, 411
AluI AGCT 3 cut(s) 165, 363, 411
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 2 cut(s) 346, 505
Ama87I CYCGRG 1 cut(s) 106
AoxI GGCC 2 cut(s) 77, 365
ApeKI GCWGC 2 cut(s) 225, 289
ApoI RAATTY 1 cut(s) 444
AspLEI GCGC 3 cut(s) 123, 240, 289
AspS9I GGNCC 2 cut(s) 77, 220
AsuC2I CCSGG 2 cut(s) 58, 313
AsuHPI GGTGA 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 106
AvaII GGWCC 1 cut(s) 220
BanI GGYRCC 1 cut(s) 120
BanII GRGCYC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 2 cut(s) 237, 276
BccI CCATC 1 cut(s) 57
BceAI ACGGC 1 cut(s) 139
BcnI CCSGG 2 cut(s) 58, 313
BcoDI GTCTC 2 cut(s) 346, 505
BfoI RGCGCY 3 cut(s) 124, 241, 290
BisI GCNGC 6 cut(s) 119, 149, 152, 226, 290, 368
BlsI GCNGC 6 cut(s) 120, 150, 153, 227, 291, 369
Bme1390I CCNGG 2 cut(s) 58, 313
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 106
BmgT120I GGNCC 2 cut(s) 77, 220
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 58, 313
BmrI ACTGGG 1 cut(s) 399
BmsI GCATC 3 cut(s) 185, 371, 479
BmuI ACTGGG 1 cut(s) 399
BpmI CTGGAG 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 166
BpuEI CTTGAG 1 cut(s) 82
BpuMI CCSGG 2 cut(s) 58, 313
BsaHI GRCGYC 1 cut(s) 121
BsaJI CCNNGG 4 cut(s) 14, 56, 80, 116
BsaXI ACNNNNNCTCC 4 cut(s) 154, 184, 330, 360
Bsc4I CCNNNNNNNGG 1 cut(s) 63
Bse1I ACTGG 2 cut(s) 394, 499
BseDI CCNNGG 4 cut(s) 14, 56, 80, 116
BseGI GGATG 1 cut(s) 20
BseLI CCNNNNNNNGG 1 cut(s) 63
BseMII CTCAG 3 cut(s) 180, 357, 362
BseNI ACTGG 2 cut(s) 394, 499
BseRI GAGGAG 2 cut(s) 124, 351
BseXI GCAGC 2 cut(s) 237, 276
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 79, 367
BshNI GGYRCC 1 cut(s) 120
BsiHKAI GWGCWC 1 cut(s) 167
BsiHKCI CYCGRG 1 cut(s) 106
BsiSI CCGG 2 cut(s) 58, 312
BslFI GGGAC 2 cut(s) 15, 37
BslI CCNNNNNNNGG 1 cut(s) 63
BsmAI GTCTC 2 cut(s) 346, 505
BsmFI GGGAC 2 cut(s) 15, 37
BsnI GGCC 2 cut(s) 79, 367
BsoBI CYCGRG 1 cut(s) 106
Bsp1286I GDGCHC 1 cut(s) 167
Bsp19I CCATGG 1 cut(s) 14
BspACI CCGC 7 cut(s) 116, 118, 149, 152, 223, 368, 417
BspANI GGCC 2 cut(s) 79, 367
BspCNI CTCAG 3 cut(s) 179, 356, 363
BspFNI CGCG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 122
BspQI GCTCTTC 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 120
BsrI ACTGG 2 cut(s) 394, 499
BssECI CCNNGG 4 cut(s) 14, 56, 80, 116
BssNI GRCGYC 1 cut(s) 121
BssT1I CCWWGG 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 401
BstACI GRCGYC 1 cut(s) 121
BstC8I GCNNGC 2 cut(s) 93, 365
BstDEI CTNAG 3 cut(s) 166, 343, 371
BstDSI CCRYGG 2 cut(s) 14, 116
BstF5I GGATG 1 cut(s) 20
BstFNI CGCG 1 cut(s) 118
BstH2I RGCGCY 3 cut(s) 124, 241, 290
BstHHI GCGC 3 cut(s) 123, 240, 289
BstMAI GTCTC 2 cut(s) 346, 505
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 56, 311
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 2 cut(s) 237, 276
BsuRI GGCC 2 cut(s) 79, 367
BtgI CCRYGG 2 cut(s) 14, 116
BtsCI GGATG 1 cut(s) 20
BtsIMutI CAGTG 2 cut(s) 351, 492
Cac8I GCNNGC 2 cut(s) 93, 365
CfoI GCGC 3 cut(s) 123, 240, 289
Cfr13I GGNCC 2 cut(s) 77, 220
Cfr42I CCGCGG 1 cut(s) 119
Csp6I GTAC 2 cut(s) 183, 309
CviAII CATG 1 cut(s) 15
CviJI RGCY 6 cut(s) 79, 103, 165, 363, 367, 411
CviKI_1 RGCY 6 cut(s) 79, 103, 165, 363, 367, 411
CviQI GTAC 2 cut(s) 183, 309
DdeI CTNAG 3 cut(s) 166, 343, 371
DinI GGCGCC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 365
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Ecl136II GAGCTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 14
Eco24I GRGCYC 1 cut(s) 167
Eco47I GGWCC 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 165
Eco88I CYCGRG 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 14
EcoT38I GRGCYC 1 cut(s) 167
EgeI GGCGCC 1 cut(s) 122
EheI GGCGCC 1 cut(s) 122
ErhI CCWWGG 1 cut(s) 14
FaeI CATG 1 cut(s) 18
FaiI YATR 6 cut(s) 16, 251, 321, 460, 483, 508
FaqI GGGAC 2 cut(s) 15, 37
FatI CATG 1 cut(s) 14
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 119, 149, 152, 226, 290, 368
FokI GGATG 1 cut(s) 7
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 6 cut(s) 119, 149, 152, 226, 290, 368
GlaI GCGC 3 cut(s) 122, 239, 288
GluI GCNGC 6 cut(s) 119, 149, 152, 226, 290, 368
GsuI CTGGAG 1 cut(s) 175
HaeII RGCGCY 3 cut(s) 124, 241, 290
HaeIII GGCC 2 cut(s) 79, 367
HapII CCGG 2 cut(s) 58, 312
HhaI GCGC 3 cut(s) 123, 240, 289
Hin1I GRCGYC 1 cut(s) 121
Hin1II CATG 1 cut(s) 18
Hin6I GCGC 3 cut(s) 121, 238, 287
HinP1I GCGC 3 cut(s) 121, 238, 287
HincII GTYRAC 2 cut(s) 6, 132
HindII GTYRAC 2 cut(s) 6, 132
HinfI GANTC 5 cut(s) 112, 339, 436, 462, 498
HpaII CCGG 2 cut(s) 58, 312
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 3 cut(s) 6, 132, 454
Hpy188I TCNGA 1 cut(s) 174
Hpy188III TCNNGA 5 cut(s) 69, 106, 160, 192, 403
Hpy8I GTNNAC 3 cut(s) 6, 132, 454
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4V TGCA 7 cut(s) 141, 253, 323, 349, 384, 471, 492
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 3 cut(s) 166, 343, 371
Hsp92I GRCGYC 1 cut(s) 121
Hsp92II CATG 1 cut(s) 18
HspAI GCGC 3 cut(s) 121, 238, 287
KasI GGCGCC 1 cut(s) 120
KspI CCGCGG 1 cut(s) 119
LguI GCTCTTC 1 cut(s) 401
LmnI GCTCC 1 cut(s) 162
Lsp1109I GCAGC 2 cut(s) 237, 276
LweI GCATC 3 cut(s) 185, 371, 479
MaeIII GTNAC 1 cut(s) 208
MboII GAAGA 3 cut(s) 58, 418, 495
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 2 cut(s) 428, 444
Mly113I GGCGCC 1 cut(s) 121
MlyI GAGTC 3 cut(s) 121, 348, 507
MnlI CCTC 7 cut(s) 43, 68, 75, 102, 106, 228, 329
MseI TTAA 1 cut(s) 413
MspA1I CMGCKG 2 cut(s) 118, 370
MspI CCGG 2 cut(s) 58, 312
MspR9I CCNGG 2 cut(s) 58, 313
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 1 cut(s) 329
NarI GGCGCC 1 cut(s) 121
NciI CCSGG 2 cut(s) 58, 313
NcoI CCATGG 1 cut(s) 14
NlaIII CATG 1 cut(s) 18
NlaIV GGNNCC 1 cut(s) 122
NmeAIII GCCGAG 1 cut(s) 105
PaeR7I CTCGAG 1 cut(s) 106
PciSI GCTCTTC 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 152
PfeI GAWTC 2 cut(s) 436, 462
PkrI GCNGC 6 cut(s) 120, 150, 153, 227, 291, 369
PleI GAGTC 3 cut(s) 120, 347, 506
PluTI GGCGCC 1 cut(s) 124
PpsI GAGTC 3 cut(s) 120, 347, 506
Psp124BI GAGCTC 1 cut(s) 167
PspN4I GGNNCC 1 cut(s) 122
PspPI GGNCC 2 cut(s) 77, 220
RsaI GTAC 2 cut(s) 184, 310
RsaNI GTAC 2 cut(s) 183, 309
SacI GAGCTC 1 cut(s) 167
SacII CCGCGG 1 cut(s) 119
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 401
SaqAI TTAA 1 cut(s) 413
SatI GCNGC 6 cut(s) 119, 149, 152, 226, 290, 368
Sau96I GGNCC 2 cut(s) 77, 220
SchI GAGTC 3 cut(s) 121, 348, 507
ScrFI CCNGG 2 cut(s) 58, 313
SduI GDGCHC 1 cut(s) 167
SetI ASST 7 cut(s) 86, 167, 172, 365, 413, 459, 470
SfaNI GCATC 3 cut(s) 185, 371, 479
SfoI GGCGCC 1 cut(s) 122
Sfr274I CTCGAG 1 cut(s) 106
Sfr303I CCGCGG 1 cut(s) 119
SgrBI CCGCGG 1 cut(s) 119
SinI GGWCC 1 cut(s) 220
SlaI CTCGAG 1 cut(s) 106
SmlI CTYRAG 2 cut(s) 97, 106
SmoI CTYRAG 2 cut(s) 97, 106
Sse9I AATT 2 cut(s) 428, 444
SsiI CCGC 7 cut(s) 116, 118, 149, 152, 223, 368, 417
SspDI GGCGCC 1 cut(s) 120
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 2 cut(s) 56, 311
StyI CCWWGG 1 cut(s) 14
TaaI ACNGT 1 cut(s) 208
TaqI TCGA 3 cut(s) 5, 68, 107
TasI AATT 2 cut(s) 428, 444
TatI WGTACW 1 cut(s) 182
TauI GCSGC 4 cut(s) 121, 151, 154, 370
TfiI GAWTC 2 cut(s) 436, 462
Tru1I TTAA 1 cut(s) 413
Tru9I TTAA 1 cut(s) 413
TscAI CASTG 2 cut(s) 351, 499
TseI GCWGC 2 cut(s) 225, 289
TspDTI ATGAA 2 cut(s) 475, 495
TspRI CASTG 2 cut(s) 351, 499
VpaK11BI GGWCC 1 cut(s) 220
XapI RAATTY 1 cut(s) 444
XhoI CTCGAG 1 cut(s) 106
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.