pycom14g11100

Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0029299
Physical Location & Seq
Reverse (-)
13924137 .. 13927087
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g11100.4

Sequence Viewer

Length: 969 bp
ATGCTCTTCGGCGGTGGAGCTGATGGTTGGAAATGGATCGTGTTCGCGGAGGCTAGCGCCGGCGGCGGCGGCGGCGGTGGTGGGGGGAGAAGCGGTTCGCTCATCGGCGGCGGCGTCGGTGGCGGAAGCGGCTTCTGGTCTTGGACTGCTTCCTCCAATGTCGGCTTAGCCGTCGCCGTCACGGCCATGGCCGGTATCGCCTTGGCCGCCACAGTCGTCTACTCTCGTAGGGGCAGTCTTAAATCACCTTGGTCCCGTAGGAGAAAAAAGCACGCCCTTCTACCAAAACAATGGAAAAGTTTATTTACACCGGATGGGAAACTCACTGATGGTGGTCTCAAATTTTTGAAGAAAGTTCGCAGTGGAGGTGTTGATCCAAGTATTCGAGCTGAAGTGTGGCCGTTTCTTCTTGGAATCTATGATGTGAACAGTTCCAAGGAAGAAAGAGACTCTGTAAAAAACCAGAAAAGAAAAGAATATGAAAACTTGCGGAGACAGTGTCGCAGAATTTTAACACAAAGTGATAAGAGCTCGAAGTTGAAGGAAACTTCTGGTAACAGTAGCACTGAGAGCAGCGGGGATTTCAGTCAAGTTTTAGATTCTTCTGACCAAGGGGATGTTACAAGTGCCAGGAGGTACATTTCAATTGAGGGAGGAGACCAATTACCCAAGGATTCAGATAACCCTGTAGGCAATCAAGCTGTTCAGACTTCTGAATCACTTGAAGGAGTTGGTGAGAAAAGTGGAGTCACCTGTGACGATCCTTATGCTGCCGATACGGAATCAACTTATTCTGATTCCTCTGAAGAAACTGATATCACTCGACCATTACTATCCACTGATGTTACTGAGGAAAACTATGTTGATGACAAGCCTAAGGAGAGTTCCTCTCTCTCCAATATTGAAAACAAGCCCAAAGTCCACTCAGCAGAAGATTTTGCCACATGGCAGAGGATCGATCCGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
RabGAP-TBC

Protein Analysis

323

Amino Acids

34.47

Weight (kDa)

5.67

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029299)

Species Orthologous Gene IDs
pyrus_communis pycom06g10990 pycom14g11100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 219
AccII CGCG 1 cut(s) 47
AclWI GGATC 5 cut(s) 44, 368, 755, 953, 962
AcoI YGGCCR 4 cut(s) 183, 189, 204, 398
AcsI RAATTY 2 cut(s) 341, 507
AcuI CTGAAG 2 cut(s) 411, 825
AcyI GRCGYC 1 cut(s) 114
AdeI CACNNNGTG 1 cut(s) 521
AfaI GTAC 1 cut(s) 638
AgsI TTSAA 5 cut(s) 349, 541, 645, 725, 905
AjnI CCWGG 1 cut(s) 629
AloI GAACNNNNNNTCC 2 cut(s) 79, 111
AluBI AGCT 4 cut(s) 20, 389, 531, 701
AluI AGCT 4 cut(s) 20, 389, 531, 701
Alw21I GWGCWC 1 cut(s) 533
Alw26I GTCTC 4 cut(s) 341, 441, 487, 651
AlwI GGATC 5 cut(s) 44, 368, 755, 953, 962
AoxI GGCC 4 cut(s) 183, 189, 204, 398
ApeKI GCWGC 2 cut(s) 573, 770
ApoI RAATTY 2 cut(s) 341, 507
Asp700I GAANNNNTTC 1 cut(s) 94
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 1 cut(s) 252
AsuHPI GGTGA 3 cut(s) 237, 742, 746
AsuNHI GCTAGC 1 cut(s) 53
AvaII GGWCC 1 cut(s) 252
AxyI CCTNAGG 1 cut(s) 876
BanII GRGCYC 1 cut(s) 533
Bbv12I GWGCWC 1 cut(s) 533
BbvI GCAGC 2 cut(s) 585, 757
BccI CCATC 3 cut(s) 17, 308, 323
BceAI ACGGC 4 cut(s) 155, 161, 198, 385
BcgI CGANNNNNNTGC 2 cut(s) 749, 783
BciT130I CCWGG 1 cut(s) 631
BcoDI GTCTC 4 cut(s) 341, 441, 487, 651
BfaI CTAG 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 687
BfoI RGCGCY 1 cut(s) 60
BglI GCCNNNNNGGC 1 cut(s) 182
BlpI GCTNAGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 631
Bme18I GGWCC 1 cut(s) 252
BmgT120I GGNCC 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 254
BmrFI CCNGG 1 cut(s) 631
BmtI GCTAGC 1 cut(s) 57
BplI GAGNNNNNCTC 4 cut(s) 872, 904, 874, 906
Bpu1102I GCTNAGC 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 957
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 2 cut(s) 341, 651
BsaJI CCNNGG 6 cut(s) 186, 201, 248, 435, 610, 669
BsaWI WCCGGW 1 cut(s) 310
BsaXI ACNNNNNCTCC 4 cut(s) 79, 109, 484, 514
Bse118I RCCGGY 2 cut(s) 59, 191
Bse21I CCTNAGG 1 cut(s) 876
BseBI CCWGG 1 cut(s) 631
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 6 cut(s) 186, 201, 248, 435, 610, 669
BseGI GGATG 2 cut(s) 319, 622
BseMII CTCAG 3 cut(s) 558, 840, 939
BseRI GAGGAG 1 cut(s) 669
BseXI GCAGC 2 cut(s) 585, 757
Bsh1236I CGCG 1 cut(s) 47
BshFI GGCC 4 cut(s) 185, 191, 206, 400
BshVI ATCGAT 1 cut(s) 957
BsiHKAI GWGCWC 1 cut(s) 533
BsiSI CCGG 3 cut(s) 60, 192, 311
BslFI GGGAC 1 cut(s) 238
BsmAI GTCTC 4 cut(s) 341, 441, 487, 651
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 4 cut(s) 185, 191, 206, 400
Bso31I GGTCTC 2 cut(s) 341, 651
Bsp1286I GDGCHC 1 cut(s) 533
Bsp143I GATC 5 cut(s) 36, 373, 760, 954, 958
Bsp1720I GCTNAGC 1 cut(s) 166
Bsp19I CCATGG 1 cut(s) 186
BspANI GGCC 4 cut(s) 185, 191, 206, 400
BspCNI CTCAG 3 cut(s) 559, 841, 938
BspDI ATCGAT 1 cut(s) 957
BspFNI CGCG 1 cut(s) 47
BspLI GGNNCC 1 cut(s) 254
BspOI GCTAGC 1 cut(s) 57
BspPI GGATC 5 cut(s) 44, 368, 755, 953, 962
BspQI GCTCTTC 1 cut(s) 11
BspTNI GGTCTC 2 cut(s) 341, 651
BsrFI RCCGGY 2 cut(s) 59, 191
BssAI RCCGGY 2 cut(s) 59, 191
BssECI CCNNGG 6 cut(s) 186, 201, 248, 435, 610, 669
BssMI GATC 5 cut(s) 36, 373, 760, 954, 958
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 6 cut(s) 186, 201, 248, 435, 610, 669
Bst2UI CCWGG 1 cut(s) 631
Bst4CI ACNGT 4 cut(s) 214, 431, 498, 560
Bst6I CTCTTC 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 114
BstC8I GCNNGC 3 cut(s) 55, 61, 273
BstDEI CTNAG 5 cut(s) 166, 567, 849, 876, 925
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 319, 622
BstFNI CGCG 1 cut(s) 47
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 5 cut(s) 39, 376, 763, 957, 961
BstMAI GTCTC 4 cut(s) 341, 441, 487, 651
BstMBI GATC 5 cut(s) 36, 373, 760, 954, 958
BstMWI GCNNNNNNNGC 9 cut(s) 63, 69, 72, 120, 129, 182, 197, 206, 570
BstNI CCWGG 1 cut(s) 631
BstSCI CCNGG 1 cut(s) 629
BstSFI CTRYAG 1 cut(s) 687
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 2 cut(s) 585, 757
BstXI CCANNNNNNTGG 1 cut(s) 291
Bsu15I ATCGAT 1 cut(s) 957
Bsu36I CCTNAGG 1 cut(s) 876
BsuRI GGCC 4 cut(s) 185, 191, 206, 400
BsuTUI ATCGAT 1 cut(s) 957
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 319, 622
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 5 cut(s) 324, 367, 503, 564, 837
Cac8I GCNNGC 3 cut(s) 55, 61, 273
CfoI GCGC 1 cut(s) 59
Cfr10I RCCGGY 2 cut(s) 59, 191
Cfr13I GGNCC 1 cut(s) 252
ClaI ATCGAT 1 cut(s) 957
CseI GACGC 1 cut(s) 103
Csp6I GTAC 1 cut(s) 637
CviAII CATG 2 cut(s) 187, 945
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 5 cut(s) 166, 567, 849, 876, 925
DpnI GATC 5 cut(s) 38, 375, 762, 956, 960
DpnII GATC 5 cut(s) 36, 373, 760, 954, 958
DraIII CACNNNGTG 1 cut(s) 521
EaeI YGGCCR 4 cut(s) 183, 189, 204, 398
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
EciI GGCGGA 2 cut(s) 138, 951
Ecl136II GAGCTC 1 cut(s) 531
Eco130I CCWWGG 6 cut(s) 186, 201, 248, 435, 610, 669
Eco24I GRGCYC 1 cut(s) 533
Eco31I GGTCTC 2 cut(s) 341, 651
Eco32I GATATC 1 cut(s) 817
Eco47I GGWCC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 531
Eco57I CTGAAG 2 cut(s) 411, 825
Eco81I CCTNAGG 1 cut(s) 876
EcoICRI GAGCTC 1 cut(s) 531
EcoRII CCWGG 1 cut(s) 629
EcoRV GATATC 1 cut(s) 817
EcoT14I CCWWGG 6 cut(s) 186, 201, 248, 435, 610, 669
EcoT38I GRGCYC 1 cut(s) 533
ErhI CCWWGG 6 cut(s) 186, 201, 248, 435, 610, 669
FaeI CATG 2 cut(s) 190, 948
FaiI YATR 6 cut(s) 188, 420, 480, 768, 861, 946
FaqI GGGAC 1 cut(s) 238
FatI CATG 2 cut(s) 186, 944
FauI CCCGC 1 cut(s) 569
FblI GTMKAC 1 cut(s) 219
FokI GGATG 2 cut(s) 326, 629
FriOI GRGCYC 1 cut(s) 533
FspBI CTAG 1 cut(s) 54
GlaI GCGC 1 cut(s) 58
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 4 cut(s) 185, 191, 206, 400
HapII CCGG 3 cut(s) 60, 192, 311
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 59
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 2 cut(s) 190, 948
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HinfI GANTC 8 cut(s) 414, 449, 599, 674, 716, 747, 782, 797
HpaII CCGG 3 cut(s) 60, 192, 311
HphI GGTGA 3 cut(s) 237, 742, 746
Hpy166II GTNNAC 3 cut(s) 220, 427, 922
Hpy188I TCNGA 6 cut(s) 607, 679, 708, 715, 796, 805
Hpy8I GTNNAC 3 cut(s) 220, 427, 922
Hpy99I CGWCG 2 cut(s) 119, 176
HpyAV CCTTC 3 cut(s) 287, 535, 719
HpyCH4III ACNGT 4 cut(s) 214, 431, 498, 560
HpyF10VI GCNNNNNNNGC 9 cut(s) 63, 69, 72, 120, 129, 182, 197, 206, 570
HpyF3I CTNAG 5 cut(s) 166, 567, 849, 876, 925
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 2 cut(s) 190, 948
HspAI GCGC 1 cut(s) 57
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Kzo9I GATC 5 cut(s) 36, 373, 760, 954, 958
LguI GCTCTTC 1 cut(s) 11
LmnI GCTCC 1 cut(s) 17
Lsp1109I GCAGC 2 cut(s) 585, 757
MaeI CTAG 1 cut(s) 54
MaeIII GTNAC 6 cut(s) 178, 554, 619, 748, 755, 844
MalI GATC 5 cut(s) 38, 375, 762, 956, 960
MboI GATC 5 cut(s) 36, 373, 760, 954, 958
MboII GAAGA 6 cut(s) 361, 398, 452, 594, 818, 944
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 1 cut(s) 533
MluCI AATT 4 cut(s) 341, 507, 645, 662
MlyI GAGTC 2 cut(s) 443, 756
MmeI TCCRAC 1 cut(s) 8
MreI CGCCGGCG 1 cut(s) 59
MroNI GCCGGC 1 cut(s) 59
MroXI GAANNNNTTC 1 cut(s) 94
MseI TTAA 2 cut(s) 240, 512
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 576
MspI CCGG 3 cut(s) 60, 192, 311
MspR9I CCNGG 1 cut(s) 631
MunI CAATTG 1 cut(s) 645
MvaI CCWGG 1 cut(s) 631
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 9 cut(s) 63, 69, 72, 120, 129, 182, 197, 206, 570
NaeI GCCGGC 1 cut(s) 61
NcoI CCATGG 1 cut(s) 186
NdeII GATC 5 cut(s) 36, 373, 760, 954, 958
NgoMIV GCCGGC 1 cut(s) 59
NheI GCTAGC 1 cut(s) 53
NlaIII CATG 2 cut(s) 190, 948
NlaIV GGNNCC 1 cut(s) 254
NmuCI GTSAC 3 cut(s) 178, 748, 755
PciSI GCTCTTC 1 cut(s) 11
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 168
PdiI GCCGGC 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 94
PfeI GAWTC 6 cut(s) 414, 599, 674, 716, 782, 797
PflFI GACNNNGTC 1 cut(s) 498
PleI GAGTC 2 cut(s) 443, 755
PpsI GAGTC 2 cut(s) 443, 755
Psp124BI GAGCTC 1 cut(s) 533
Psp6I CCWGG 1 cut(s) 629
PspGI CCWGG 1 cut(s) 629
PspN4I GGNNCC 1 cut(s) 254
PspPI GGNCC 1 cut(s) 252
PsyI GACNNNGTC 1 cut(s) 498
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 1 cut(s) 533
SapI GCTCTTC 1 cut(s) 11
SaqAI TTAA 2 cut(s) 240, 512
Sau3AI GATC 5 cut(s) 36, 373, 760, 954, 958
Sau96I GGNCC 1 cut(s) 252
SchI GAGTC 2 cut(s) 443, 756
ScrFI CCNGG 1 cut(s) 631
SduI GDGCHC 1 cut(s) 533
SetI ASST 8 cut(s) 22, 250, 370, 391, 533, 638, 703, 755
SfcI CTRYAG 1 cut(s) 687
SgrAI CRCCGGYG 1 cut(s) 59
SinI GGWCC 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 4 cut(s) 341, 507, 645, 662
SspI AATATT 1 cut(s) 901
SspMI CTAG 1 cut(s) 54
SstI GAGCTC 1 cut(s) 533
StyD4I CCNGG 1 cut(s) 629
StyI CCWWGG 6 cut(s) 186, 201, 248, 435, 610, 669
TaaI ACNGT 4 cut(s) 214, 431, 498, 560
TaqI TCGA 4 cut(s) 385, 533, 823, 957
TasI AATT 4 cut(s) 341, 507, 645, 662
TauI GCSGC 8 cut(s) 66, 69, 72, 75, 111, 114, 132, 209
TfiI GAWTC 6 cut(s) 414, 599, 674, 716, 782, 797
Tru1I TTAA 2 cut(s) 240, 512
Tru9I TTAA 2 cut(s) 240, 512
TscAI CASTG 5 cut(s) 331, 367, 503, 571, 844
TseFI GTSAC 3 cut(s) 178, 748, 755
TseI GCWGC 2 cut(s) 573, 770
Tsp45I GTSAC 3 cut(s) 178, 748, 755
TspDTI ATGAA 1 cut(s) 495
TspGWI ACGGA 1 cut(s) 794
TspRI CASTG 5 cut(s) 331, 367, 503, 571, 844
Tth111I GACNNNGTC 1 cut(s) 498
VpaK11BI GGWCC 1 cut(s) 252
XapI RAATTY 2 cut(s) 341, 507
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 94
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.