pycom15g00200

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012551
Physical Location & Seq
Forward (+)
119085 .. 119708
624 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 624 bp
ATGGGTTGCGCCGCCTCAAAGGTAGAGAACCTTCCGGCGGTGGCATTGTGCCGAGACCGGTGCAAGTACCTGGAGGAAGCTCTCTGCCAGGCCCAAGCCCTGGCCGATTCCCACGTCGCTTACTTAGCCTCTCTCAGGACCCTAGGCCCCGTTCTCCACCGTTTCTTCCATGACATTCCTGCAGGTACTGAACCGCCGCCGGCGCCACCAGAATCAGACTCGCACCTCCAATTCCCTTCCGACTCTGAATCCGAATACAAAGAGGACCACACTGATAAACAGTTGGGAGAAACGCCGACACCAGTTTCTGTGTATGATCAATGTGATCAATGTTTCAGGAAAAGGGAGGAGGAGGTCTTCAAAAATTTAGGCGGCAGACTGACTTCCAAACTCCCTCCTTCACCACCGCCTCCGGTCAGCTCCACTTGGGATTTTCTTAATCTCTTTGATGCCTACGAGAGGGAGAGTTACCTAGCACCCCTTTATACTTGCACTACTTTGGTTCCTGAAGAAGCTGCTCATACTTCTGCACTTGCTGCTGCCGCTGCTGACAAAATCAATAACGAGATCAATGTTAAACACAATACTAAAAATATTGACGATCGATCAGGAAAGGGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

208

Amino Acids

22.8

Weight (kDa)

5.0

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 1 - 57 5.1e-22 Protein of unknown function (DUF630)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20530
fragaria_vesca FvH4_2g37590 FvH4_2g37590
malus_domestica MD08G1003500.v1.1 MD15G1003100.v1.1
prunus_persica Prupe.1G355400_v2.0.a1
pyrus_communis pycom08g00270 pycom15g00200 pycom15g00210
rosa_chinensis RchiOBHm_Chr6g0304141
rosa_laevigata RLG00000011011
rosa_multiflora Rmu_ssc0000218.1_g000006
rosa_roxburghii Rroxscaffold_7G00164030
rosa_rugosa Rorug06G0328000
rosa_samantha Rh6AG442000 Rh6BG460800 Rh6CG454200 Rh6DG441400
rosa_wichuraiana Rw6G038310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 173
AccB1I GGYRCC 1 cut(s) 202
AccB7I CCANNNNNTGG 1 cut(s) 100
AciI CCGC 7 cut(s) 12, 38, 194, 197, 372, 407, 543
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 364
AcuI CTGAAG 1 cut(s) 528
AcyI GRCGYC 1 cut(s) 203
AfaI GTAC 2 cut(s) 68, 187
AfiI CCNNNNNNNGG 4 cut(s) 37, 100, 135, 459
AgeI ACCGGT 1 cut(s) 57
AgsI TTSAA 1 cut(s) 361
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 3 cut(s) 69, 87, 99
AluBI AGCT 3 cut(s) 80, 420, 515
AluI AGCT 3 cut(s) 80, 420, 515
Alw26I GTCTC 1 cut(s) 48
AlwNI CAGNNNCTG 2 cut(s) 188, 308
AoxI GGCC 3 cut(s) 90, 102, 145
ApeKI GCWGC 4 cut(s) 515, 536, 539, 545
ApoI RAATTY 1 cut(s) 364
AsiGI ACCGGT 1 cut(s) 57
AspA2I CCTAGG 1 cut(s) 142
AspLEI GCGC 2 cut(s) 11, 205
AspS9I GGNCC 4 cut(s) 91, 138, 146, 265
AsuHPI GGTGA 1 cut(s) 393
AvaII GGWCC 2 cut(s) 138, 265
AvrII CCTAGG 1 cut(s) 142
BanI GGYRCC 1 cut(s) 202
BarI GAAGNNNNNNTAC 2 cut(s) 15, 47
BbsI GAAGAC 1 cut(s) 349
BbvI GCAGC 4 cut(s) 502, 523, 526, 532
BcgI CGANNNNNNTGC 2 cut(s) 42, 76
BciT130I CCWGG 3 cut(s) 71, 89, 101
BclI TGATCA 2 cut(s) 316, 325
BcoDI GTCTC 1 cut(s) 48
BfaI CTAG 2 cut(s) 143, 473
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 1 cut(s) 206
BfuAI ACCTGC 1 cut(s) 173
BisI GCNGC 8 cut(s) 12, 197, 373, 516, 537, 540, 543, 546
BlnI CCTAGG 1 cut(s) 142
BlsI GCNGC 8 cut(s) 13, 198, 374, 517, 538, 541, 544, 547
Bme1390I CCNGG 3 cut(s) 71, 89, 101
Bme18I GGWCC 2 cut(s) 138, 265
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 4 cut(s) 91, 138, 146, 265
BmiI GGNNCC 4 cut(s) 140, 148, 204, 504
BmrFI CCNGG 3 cut(s) 71, 89, 101
BmsI GCATC 1 cut(s) 439
BpiI GAAGAC 1 cut(s) 349
BpmI CTGGAG 1 cut(s) 92
Bsa29I ATCGAT 1 cut(s) 604
BsaHI GRCGYC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 48
BsaJI CCNNGG 2 cut(s) 99, 142
BsaWI WCCGGW 2 cut(s) 57, 412
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 100, 135, 459
Bse118I RCCGGY 2 cut(s) 57, 199
Bse1I ACTGG 1 cut(s) 302
BseBI CCWGG 3 cut(s) 71, 89, 101
BseCI ATCGAT 1 cut(s) 604
BseDI CCNNGG 2 cut(s) 99, 142
BseLI CCNNNNNNNGG 4 cut(s) 37, 100, 135, 459
BseMII CTCAG 1 cut(s) 148
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 2 cut(s) 362, 365
BseXI GCAGC 4 cut(s) 502, 523, 526, 532
BsgI GTGCAG 1 cut(s) 513
Bsh1285I CGRYCG 1 cut(s) 604
BshFI GGCC 3 cut(s) 92, 104, 147
BshNI GGYRCC 1 cut(s) 202
BshTI ACCGGT 1 cut(s) 57
BshVI ATCGAT 1 cut(s) 604
BsiEI CGRYCG 1 cut(s) 604
BsiSI CCGG 4 cut(s) 35, 58, 200, 413
BslI CCNNNNNNNGG 4 cut(s) 37, 100, 135, 459
BsmAI GTCTC 1 cut(s) 48
BsnI GGCC 3 cut(s) 92, 104, 147
Bso31I GGTCTC 1 cut(s) 48
Bsp143I GATC 5 cut(s) 316, 325, 567, 601, 605
BspACI CCGC 7 cut(s) 12, 38, 194, 197, 372, 407, 543
BspANI GGCC 3 cut(s) 92, 104, 147
BspCNI CTCAG 1 cut(s) 147
BspDI ATCGAT 1 cut(s) 604
BspLI GGNNCC 4 cut(s) 140, 148, 204, 504
BspMAI CTGCAG 1 cut(s) 184
BspMI ACCTGC 1 cut(s) 173
BspT107I GGYRCC 1 cut(s) 202
BspTNI GGTCTC 1 cut(s) 48
BsrFI RCCGGY 2 cut(s) 57, 199
BsrI ACTGG 1 cut(s) 302
BssAI RCCGGY 2 cut(s) 57, 199
BssECI CCNNGG 2 cut(s) 99, 142
BssMI GATC 5 cut(s) 316, 325, 567, 601, 605
BssNI GRCGYC 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 3 cut(s) 71, 89, 101
Bst4CI ACNGT 2 cut(s) 161, 282
BstACI GRCGYC 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 536
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 2 cut(s) 124, 134
BstENI CCTNNNNNAGG 2 cut(s) 133, 457
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 2 cut(s) 11, 205
BstKTI GATC 5 cut(s) 319, 328, 570, 604, 608
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 5 cut(s) 316, 325, 567, 601, 605
BstMCI CGRYCG 1 cut(s) 604
BstMWI GCNNNNNNNGC 5 cut(s) 125, 202, 536, 542, 545
BstNI CCWGG 3 cut(s) 71, 89, 101
BstSCI CCNGG 3 cut(s) 69, 87, 99
BstSFI CTRYAG 1 cut(s) 180
BstV1I GCAGC 4 cut(s) 502, 523, 526, 532
BstV2I GAAGAC 1 cut(s) 349
Bsu15I ATCGAT 1 cut(s) 604
BsuRI GGCC 3 cut(s) 92, 104, 147
BsuTUI ATCGAT 1 cut(s) 604
BtrI CACGTC 1 cut(s) 115
BtsIMutI CAGTG 1 cut(s) 270
BveI ACCTGC 1 cut(s) 173
Cac8I GCNNGC 1 cut(s) 201
CaiI CAGNNNCTG 2 cut(s) 188, 308
CfoI GCGC 2 cut(s) 11, 205
Cfr10I RCCGGY 2 cut(s) 57, 199
Cfr13I GGNCC 4 cut(s) 91, 138, 146, 265
ClaI ATCGAT 1 cut(s) 604
Csp6I GTAC 2 cut(s) 67, 186
CspAI ACCGGT 1 cut(s) 57
CviAII CATG 1 cut(s) 170
CviJI RGCY 8 cut(s) 80, 92, 98, 104, 128, 147, 420, 515
CviKI_1 RGCY 8 cut(s) 80, 92, 98, 104, 128, 147, 420, 515
CviQI GTAC 2 cut(s) 67, 186
DdeI CTNAG 2 cut(s) 124, 134
DinI GGCGCC 1 cut(s) 204
DpnI GATC 5 cut(s) 318, 327, 569, 603, 607
DpnII GATC 5 cut(s) 316, 325, 567, 601, 605
EaeI YGGCCR 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 142
Eco31I GGTCTC 1 cut(s) 48
Eco47I GGWCC 2 cut(s) 138, 265
Eco57I CTGAAG 1 cut(s) 528
EcoNI CCTNNNNNAGG 2 cut(s) 133, 457
EcoO109I RGGNCCY 2 cut(s) 138, 146
EcoRII CCWGG 3 cut(s) 69, 87, 99
EcoT14I CCWWGG 1 cut(s) 142
EgeI GGCGCC 1 cut(s) 204
EheI GGCGCC 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 1 cut(s) 173
FaiI YATR 4 cut(s) 171, 315, 486, 522
FatI CATG 1 cut(s) 169
FbaI TGATCA 2 cut(s) 316, 325
Fnu4HI GCNGC 8 cut(s) 12, 197, 373, 516, 537, 540, 543, 546
Fsp4HI GCNGC 8 cut(s) 12, 197, 373, 516, 537, 540, 543, 546
FspBI CTAG 2 cut(s) 143, 473
GlaI GCGC 2 cut(s) 10, 204
GluI GCNGC 8 cut(s) 12, 197, 373, 516, 537, 540, 543, 546
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 206
HaeIII GGCC 3 cut(s) 92, 104, 147
HapII CCGG 4 cut(s) 35, 58, 200, 413
HhaI GCGC 2 cut(s) 11, 205
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 1 cut(s) 173
Hin6I GCGC 2 cut(s) 9, 203
HinP1I GCGC 2 cut(s) 9, 203
HinfI GANTC 5 cut(s) 107, 212, 218, 242, 248
HpaII CCGG 4 cut(s) 35, 58, 200, 413
HphI GGTGA 1 cut(s) 393
Hpy188I TCNGA 4 cut(s) 217, 241, 247, 253
Hpy188III TCNNGA 4 cut(s) 136, 337, 506, 609
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 3 cut(s) 41, 246, 408
HpyCH4III ACNGT 2 cut(s) 161, 282
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 4 cut(s) 63, 182, 492, 530
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 202, 536, 542, 545
HpyF3I CTNAG 2 cut(s) 124, 134
HpySE526I ACGT 1 cut(s) 114
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 1 cut(s) 173
HspAI GCGC 2 cut(s) 9, 203
KasI GGCGCC 1 cut(s) 202
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Ksp22I TGATCA 2 cut(s) 316, 325
Kzo9I GATC 5 cut(s) 316, 325, 567, 601, 605
LmnI GCTCC 1 cut(s) 425
Lsp1109I GCAGC 4 cut(s) 502, 523, 526, 532
LweI GCATC 1 cut(s) 439
MaeI CTAG 2 cut(s) 143, 473
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 1 cut(s) 467
MalI GATC 5 cut(s) 318, 327, 569, 603, 607
MboI GATC 5 cut(s) 316, 325, 567, 601, 605
MboII GAAGA 3 cut(s) 157, 349, 521
MluCI AATT 2 cut(s) 230, 364
Mly113I GGCGCC 1 cut(s) 203
MlyI GAGTC 2 cut(s) 212, 236
MmeI TCCRAC 1 cut(s) 264
MreI CGCCGGCG 1 cut(s) 199
MroNI GCCGGC 1 cut(s) 199
MseI TTAA 2 cut(s) 438, 576
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 4 cut(s) 35, 58, 200, 413
MspR9I CCNGG 3 cut(s) 71, 89, 101
MvaI CCWGG 3 cut(s) 71, 89, 101
MwoI GCNNNNNNNGC 5 cut(s) 125, 202, 536, 542, 545
NaeI GCCGGC 1 cut(s) 201
NarI GGCGCC 1 cut(s) 203
NdeII GATC 5 cut(s) 316, 325, 567, 601, 605
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 1 cut(s) 173
NlaIV GGNNCC 4 cut(s) 140, 148, 204, 504
NmeAIII GCCGAG 1 cut(s) 77
PdiI GCCGGC 1 cut(s) 201
PfeI GAWTC 3 cut(s) 107, 212, 248
PflMI CCANNNNNTGG 1 cut(s) 100
PinAI ACCGGT 1 cut(s) 57
PkrI GCNGC 8 cut(s) 13, 198, 374, 517, 538, 541, 544, 547
Ple19I CGATCG 1 cut(s) 604
PleI GAGTC 2 cut(s) 212, 236
PluTI GGCGCC 1 cut(s) 206
PpsI GAGTC 2 cut(s) 212, 236
PpuMI RGGWCCY 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 138
Psp6I CCWGG 3 cut(s) 69, 87, 99
PspGI CCWGG 3 cut(s) 69, 87, 99
PspN4I GGNNCC 4 cut(s) 140, 148, 204, 504
PspPI GGNCC 4 cut(s) 91, 138, 146, 265
PspPPI RGGWCCY 1 cut(s) 138
PstI CTGCAG 1 cut(s) 184
PstNI CAGNNNCTG 2 cut(s) 188, 308
PvuI CGATCG 1 cut(s) 604
RsaI GTAC 2 cut(s) 68, 187
RsaNI GTAC 2 cut(s) 67, 186
SaqAI TTAA 2 cut(s) 438, 576
SatI GCNGC 8 cut(s) 12, 197, 373, 516, 537, 540, 543, 546
Sau3AI GATC 5 cut(s) 316, 325, 567, 601, 605
Sau96I GGNCC 4 cut(s) 91, 138, 146, 265
SbfI CCTGCAGG 1 cut(s) 184
SchI GAGTC 2 cut(s) 212, 236
ScrFI CCNGG 3 cut(s) 71, 89, 101
SdaI CCTGCAGG 1 cut(s) 184
SfaNI GCATC 1 cut(s) 439
SfcI CTRYAG 1 cut(s) 180
SfoI GGCGCC 1 cut(s) 204
SgrAI CRCCGGYG 1 cut(s) 199
SinI GGWCC 2 cut(s) 138, 265
Sse8387I CCTGCAGG 1 cut(s) 184
Sse9I AATT 2 cut(s) 230, 364
SsiI CCGC 7 cut(s) 12, 38, 194, 197, 372, 407, 543
SspDI GGCGCC 1 cut(s) 202
SspI AATATT 1 cut(s) 595
SspMI CTAG 2 cut(s) 143, 473
StyD4I CCNGG 3 cut(s) 69, 87, 99
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 161, 282
TaiI ACGT 1 cut(s) 117
TaqI TCGA 1 cut(s) 604
TasI AATT 2 cut(s) 230, 364
TauI GCSGC 4 cut(s) 14, 199, 375, 545
TfiI GAWTC 3 cut(s) 107, 212, 248
Tru1I TTAA 2 cut(s) 438, 576
Tru9I TTAA 2 cut(s) 438, 576
TscAI CASTG 1 cut(s) 277
TseI GCWGC 4 cut(s) 515, 536, 539, 545
TspRI CASTG 1 cut(s) 277
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 138, 265
XagI CCTNNNNNAGG 2 cut(s) 133, 457
XapI RAATTY 1 cut(s) 364
XmaJI CCTAGG 1 cut(s) 142
XspI CTAG 2 cut(s) 143, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.