pycom15g00210

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012551
Physical Location & Seq
Forward (+)
119806 .. 121329
1524 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1224 bp
ATGCGAGAGCTTCATCTTCTGTTTGACAAGGCGTCTGAGTCCGGTAATCAAGTTGTATTGTCGCTGCGGTCTCATCATCTCACAATTTCTCTTGATCAGGAGTATGGATTTGATGACCATGAAGGCTTGGTTATGAGTTCACAGAACCTTTCTTACACTCTGAAGACGCTCTTCCTGTGGGAAAAGAAACTTTACCATGAAGTCAAGGCTGAGGAAAGATTGCGCGTTACGCACGAGAAGAAGAGTAGAATGCTGAAACATTTGGAACATAGCAAGGGTACTACTACTAGTGCTGCTGAGTCTCCCAAACTCAACTCCCTTCGCAGTGCAGTCCGCGATCTTTTAACAAAAATGAAAATTGCAATTCAGATTGTTGACAGAATATCAATTACCATCAATAAGTTGAGGGACGAGGAGCTATGGCCGCAGATTATCGAATTCATCAACAGGTTACTCAACATGTGGAAAGCTATGGTAGAATGTCACAAGAGTCAGTACCGAGCAATGGTGGAAGCCAAGGGTTTAGATGGCGTTGCAATATCCAATTCAAAATTGAGCGGTGCGCATAAGGCTGAAACTGCAATTCAACTCAAGTTAGAGCTTCAAAACTGGAATGTGTGCTTCTCCAGATGGATTGTCGCCCAAAAGGGGTATGTAAAAGCATTAAATGGATGGCTTCTGAGATGTCTTCTACACGAACCGAATGTGGGCGATCAAGAATTAACAGCGGACGAGGGTATTGGAGCACCGCCTGTTTTTGTGATGTGTAATGAGTGGTCACAAGCTATGGATAGACTCTCAGAGAAGGAAGTTGTTGAAGCCATCAGGGGATTCTGGACTAGTATACATACGCTCTTAATGGAAGACAACAATAATAATGTAGAAATGGTGCAGCAAAGGATGGTAGCTGAAAACAAAGATATAGCAAGGAAAGTAAAGTTTTTGGAGGAAGAGGAGGGGAAGGAGCAGAAGATATTGCATAAGCTGATGCGCGGTAGAGGAGGGAAAAAGAACGTTGGTTCGAGTAGTTTGCGAGCAGGAGAAGACGAGTCACACGGAGAGAGAGTGCAGCAACAGAGTAGGAGTGATCTAGTGTGGGATCTGAAGCAGACATTGATGGCGATGGAGAAATTCACTGCAAACTCATTGCAAGCATACAATGAGCTTCATGCACACATTGAACTAGAGCAAGTCAGCTGCAGGCAATCTGGAATATCTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

408

Amino Acids

46.6

Weight (kDa)

6.98

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 36 - 283 3e-84 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20530
fragaria_vesca FvH4_2g37590 FvH4_2g37590
malus_domestica MD08G1003500.v1.1 MD15G1003100.v1.1
prunus_persica Prupe.1G355400_v2.0.a1
pyrus_communis pycom08g00270 pycom15g00200 pycom15g00210
rosa_chinensis RchiOBHm_Chr6g0304141
rosa_laevigata RLG00000011011
rosa_multiflora Rmu_ssc0000218.1_g000006
rosa_roxburghii Rroxscaffold_7G00164030
rosa_rugosa Rorug06G0328000
rosa_samantha Rh6AG442000 Rh6BG460800 Rh6CG454200 Rh6DG441400
rosa_wichuraiana Rw6G038310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 564
AccBSI CCGCTC 1 cut(s) 558
AccI GTMKAC 1 cut(s) 844
AccII CGCG 3 cut(s) 225, 336, 993
AciI CCGC 7 cut(s) 67, 334, 425, 558, 728, 749, 993
AclI AACGTT 1 cut(s) 1014
AclWI GGATC 1 cut(s) 1107
AcoI YGGCCR 1 cut(s) 422
AcsI RAATTY 2 cut(s) 437, 1130
AcuI CTGAAG 2 cut(s) 182, 1124
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 2 cut(s) 280, 497
AfiI CCNNNNNNNGG 3 cut(s) 505, 648, 707
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 5 cut(s) 549, 587, 605, 818, 1181
AhdI GACNNNNNGTC 1 cut(s) 31
AhlI ACTAGT 2 cut(s) 287, 839
AluBI AGCT 9 cut(s) 10, 418, 470, 601, 785, 908, 985, 1165, 1197
AluI AGCT 9 cut(s) 10, 418, 470, 601, 785, 908, 985, 1165, 1197
Alw21I GWGCWC 1 cut(s) 748
Alw26I GTCTC 2 cut(s) 75, 306
AlwI GGATC 1 cut(s) 1107
AoxI GGCC 1 cut(s) 422
ApeKI GCWGC 5 cut(s) 64, 293, 892, 1069, 1197
ApoI RAATTY 2 cut(s) 437, 1130
AspLEI GCGC 3 cut(s) 225, 565, 993
BauI CACGAG 1 cut(s) 233
BbsI GAAGAC 4 cut(s) 170, 680, 870, 1050
Bbv12I GWGCWC 1 cut(s) 748
BbvCI CCTCAGC 1 cut(s) 210
BbvI GCAGC 5 cut(s) 51, 280, 904, 1081, 1184
BccI CCATC 8 cut(s) 401, 521, 624, 666, 830, 895, 1111, 1117
BcgI CGANNNNNNTGC 2 cut(s) 1012, 1046
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 2 cut(s) 75, 306
BcuI ACTAGT 2 cut(s) 287, 839
BfaI CTAG 4 cut(s) 288, 840, 1091, 1184
BfmI CTRYAG 1 cut(s) 1198
BisI GCNGC 6 cut(s) 65, 294, 425, 893, 1070, 1198
BlsI GCNGC 6 cut(s) 66, 295, 426, 894, 1071, 1199
BmeRI GACNNNNNGTC 1 cut(s) 31
BmsI GCATC 1 cut(s) 978
BpiI GAAGAC 4 cut(s) 170, 680, 870, 1050
BpmI CTGGAG 1 cut(s) 610
Bpu10I CCTNAGC 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 575
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 516
BsaWI WCCGGW 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 1050, 1080
Bsc4I CCNNNNNNNGG 3 cut(s) 505, 648, 707
Bse1I ACTGG 1 cut(s) 614
Bse3DI GCAATG 2 cut(s) 510, 1145
BseDI CCNNGG 1 cut(s) 516
BseGI GGATG 2 cut(s) 677, 906
BseLI CCNNNNNNNGG 3 cut(s) 505, 648, 707
BseMI GCAATG 2 cut(s) 510, 1145
BseMII CTCAG 5 cut(s) 27, 201, 288, 671, 813
BseNI ACTGG 1 cut(s) 614
BseRI GAGGAG 3 cut(s) 428, 968, 1014
BseXI GCAGC 5 cut(s) 51, 280, 904, 1081, 1184
BsgI GTGCAG 3 cut(s) 348, 911, 1088
Bsh1236I CGCG 3 cut(s) 225, 336, 993
BshFI GGCC 1 cut(s) 424
BsiHKAI GWGCWC 1 cut(s) 748
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 422
BslI CCNNNNNNNGG 3 cut(s) 505, 648, 707
BsmAI GTCTC 2 cut(s) 75, 306
BsmFI GGGAC 1 cut(s) 422
BsmI GAATGC 1 cut(s) 255
BsnI GGCC 1 cut(s) 424
Bso31I GGTCTC 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 748
Bsp143I GATC 5 cut(s) 94, 337, 712, 1087, 1099
BspACI CCGC 7 cut(s) 67, 334, 425, 558, 728, 749, 993
BspANI GGCC 1 cut(s) 424
BspCNI CTCAG 5 cut(s) 28, 202, 289, 672, 812
BspFNI CGCG 3 cut(s) 225, 336, 993
BspMAI CTGCAG 1 cut(s) 1202
BspPI GGATC 1 cut(s) 1107
BspQI GCTCTTC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 75
BsrBI CCGCTC 1 cut(s) 558
BsrDI GCAATG 2 cut(s) 510, 1145
BsrI ACTGG 1 cut(s) 614
BssECI CCNNGG 1 cut(s) 516
BssMI GATC 5 cut(s) 94, 337, 712, 1087, 1099
BssNAI GTATAC 1 cut(s) 845
BssNI GRCGYC 1 cut(s) 32
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 516
Bst1107I GTATAC 1 cut(s) 845
Bst2BI CACGAG 1 cut(s) 233
Bst6I CTCTTC 3 cut(s) 176, 236, 945
BstACI GRCGYC 1 cut(s) 32
BstC8I GCNNGC 3 cut(s) 1035, 1152, 1202
BstDEI CTNAG 5 cut(s) 36, 210, 297, 680, 799
BstF5I GGATG 2 cut(s) 677, 906
BstFNI CGCG 3 cut(s) 225, 336, 993
BstHHI GCGC 3 cut(s) 225, 565, 993
BstKTI GATC 5 cut(s) 97, 340, 715, 1090, 1102
BstMAI GTCTC 2 cut(s) 75, 306
BstMBI GATC 5 cut(s) 94, 337, 712, 1087, 1099
BstMWI GCNNNNNNNGC 4 cut(s) 229, 424, 569, 578
BstNSI RCATGY 1 cut(s) 463
BstSFI CTRYAG 1 cut(s) 1198
BstUI CGCG 3 cut(s) 225, 336, 993
BstV1I GCAGC 5 cut(s) 51, 280, 904, 1081, 1184
BstV2I GAAGAC 4 cut(s) 170, 680, 870, 1050
BstX2I RGATCY 1 cut(s) 1099
BstYI RGATCY 1 cut(s) 1099
BstZ17I GTATAC 1 cut(s) 845
BsuRI GGCC 1 cut(s) 424
BtgZI GCGATG 1 cut(s) 1136
BtsCI GGATG 2 cut(s) 677, 906
BtsI GCAGTG 2 cut(s) 331, 1134
BtsIMutI CAGTG 2 cut(s) 331, 1134
Cac8I GCNNGC 3 cut(s) 1035, 1152, 1202
CfoI GCGC 3 cut(s) 225, 565, 993
CseI GACGC 2 cut(s) 21, 175
Csp6I GTAC 2 cut(s) 279, 496
CviAII CATG 4 cut(s) 119, 197, 460, 1169
CviQI GTAC 2 cut(s) 279, 496
DdeI CTNAG 5 cut(s) 36, 210, 297, 680, 799
DpnI GATC 5 cut(s) 96, 339, 714, 1089, 1101
DpnII GATC 5 cut(s) 94, 337, 712, 1087, 1099
DriI GACNNNNNGTC 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 422
Eam1104I CTCTTC 3 cut(s) 176, 236, 945
Eam1105I GACNNNNNGTC 1 cut(s) 31
EarI CTCTTC 3 cut(s) 176, 236, 945
Eco130I CCWWGG 1 cut(s) 516
Eco31I GGTCTC 1 cut(s) 75
Eco57I CTGAAG 2 cut(s) 182, 1124
EcoRI GAATTC 1 cut(s) 437
EcoT14I CCWWGG 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 516
FaeI CATG 4 cut(s) 122, 200, 463, 1172
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FaqI GGGAC 1 cut(s) 422
FatI CATG 4 cut(s) 118, 196, 459, 1168
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 1 cut(s) 844
Fnu4HI GCNGC 6 cut(s) 65, 294, 425, 893, 1070, 1198
FokI GGATG 2 cut(s) 684, 913
Fsp4HI GCNGC 6 cut(s) 65, 294, 425, 893, 1070, 1198
FspAI RTGCGCAY 1 cut(s) 564
FspBI CTAG 4 cut(s) 288, 840, 1091, 1184
FspI TGCGCA 1 cut(s) 564
GlaI GCGC 3 cut(s) 224, 564, 992
GluI GCNGC 6 cut(s) 65, 294, 425, 893, 1070, 1198
GsuI CTGGAG 1 cut(s) 610
HaeIII GGCC 1 cut(s) 424
HapII CCGG 1 cut(s) 42
HgaI GACGC 2 cut(s) 21, 175
HhaI GCGC 3 cut(s) 225, 565, 993
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 4 cut(s) 122, 200, 463, 1172
Hin6I GCGC 3 cut(s) 223, 563, 991
HinP1I GCGC 3 cut(s) 223, 563, 991
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HinfI GANTC 6 cut(s) 38, 299, 490, 795, 831, 1049
HpaII CCGG 1 cut(s) 42
Hpy166II GTNNAC 3 cut(s) 140, 376, 845
Hpy188I TCNGA 6 cut(s) 37, 162, 369, 681, 802, 1104
Hpy188III TCNNGA 7 cut(s) 92, 98, 627, 716, 835, 1209, 1218
Hpy8I GTNNAC 3 cut(s) 140, 376, 845
HpyAV CCTTC 4 cut(s) 116, 329, 799, 955
HpyCH4IV ACGT 1 cut(s) 1014
HpyF10VI GCNNNNNNNGC 4 cut(s) 229, 424, 569, 578
HpyF3I CTNAG 5 cut(s) 36, 210, 297, 680, 799
HpySE526I ACGT 1 cut(s) 1014
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 4 cut(s) 122, 200, 463, 1172
HspAI GCGC 3 cut(s) 223, 563, 991
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 5 cut(s) 94, 337, 712, 1087, 1099
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 3 cut(s) 415, 743, 964
Lsp1109I GCAGC 5 cut(s) 51, 280, 904, 1081, 1184
LweI GCATC 1 cut(s) 978
MaeI CTAG 4 cut(s) 288, 840, 1091, 1184
MaeII ACGT 1 cut(s) 1014
MaeIII GTNAC 5 cut(s) 226, 450, 482, 777, 1050
MalI GATC 5 cut(s) 96, 339, 714, 1089, 1101
MbiI CCGCTC 1 cut(s) 558
MboI GATC 5 cut(s) 94, 337, 712, 1087, 1099
MflI RGATCY 1 cut(s) 1099
MhlI GDGCHC 1 cut(s) 748
MlyI GAGTC 5 cut(s) 47, 308, 499, 789, 1058
MnlI CCTC 9 cut(s) 205, 399, 406, 727, 940, 946, 949, 992, 995
MseI TTAA 4 cut(s) 344, 665, 722, 857
MspA1I CMGCKG 2 cut(s) 728, 1197
MspI CCGG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 255
MvnI CGCG 3 cut(s) 225, 336, 993
MwoI GCNNNNNNNGC 4 cut(s) 229, 424, 569, 578
NdeII GATC 5 cut(s) 94, 337, 712, 1087, 1099
NlaIII CATG 4 cut(s) 122, 200, 463, 1172
NmuCI GTSAC 3 cut(s) 482, 777, 1050
NsbI TGCGCA 1 cut(s) 564
NspI RCATGY 1 cut(s) 463
PciI ACATGT 1 cut(s) 459
PciSI GCTCTTC 1 cut(s) 176
PctI GAATGC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 831
PkrI GCNGC 6 cut(s) 66, 295, 426, 894, 1071, 1199
PleI GAGTC 5 cut(s) 46, 307, 498, 789, 1057
PpsI GAGTC 5 cut(s) 46, 307, 498, 789, 1057
PscI ACATGT 1 cut(s) 459
Psp1406I AACGTT 1 cut(s) 1014
PsrI GAACNNNNNNTAC 2 cut(s) 137, 169
PstI CTGCAG 1 cut(s) 1202
PsuI RGATCY 1 cut(s) 1099
PvuII CAGCTG 1 cut(s) 1197
RsaI GTAC 2 cut(s) 280, 497
RsaNI GTAC 2 cut(s) 279, 496
SapI GCTCTTC 1 cut(s) 176
SaqAI TTAA 4 cut(s) 344, 665, 722, 857
SatI GCNGC 6 cut(s) 65, 294, 425, 893, 1070, 1198
Sau3AI GATC 5 cut(s) 94, 337, 712, 1087, 1099
SchI GAGTC 5 cut(s) 47, 308, 499, 789, 1058
SduI GDGCHC 1 cut(s) 748
SfaNI GCATC 1 cut(s) 978
SfcI CTRYAG 1 cut(s) 1198
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
SpeI ACTAGT 2 cut(s) 287, 839
SsiI CCGC 7 cut(s) 67, 334, 425, 558, 728, 749, 993
SspMI CTAG 4 cut(s) 288, 840, 1091, 1184
StyI CCWWGG 1 cut(s) 516
TaiI ACGT 1 cut(s) 1017
TaqI TCGA 2 cut(s) 435, 1022
TauI GCSGC 1 cut(s) 427
TfiI GAWTC 1 cut(s) 831
Tru1I TTAA 4 cut(s) 344, 665, 722, 857
Tru9I TTAA 4 cut(s) 344, 665, 722, 857
TscAI CASTG 2 cut(s) 331, 1141
TseFI GTSAC 3 cut(s) 482, 777, 1050
TseI GCWGC 5 cut(s) 64, 293, 892, 1069, 1197
Tsp45I GTSAC 3 cut(s) 482, 777, 1050
TspDTI ATGAA 5 cut(s) 135, 213, 368, 430, 1157
TspGWI ACGGA 1 cut(s) 1071
TspRI CASTG 2 cut(s) 331, 1141
XapI RAATTY 2 cut(s) 437, 1130
XceI RCATGY 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 844
XspI CTAG 4 cut(s) 288, 840, 1091, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.