pycom15g02510

Acts in the modification of cell walls via demethylesterification of cell wall pectin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0028583
Physical Location & Seq
Forward (+)
1536490 .. 1537035
546 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g02510.1

Sequence Viewer

Length: 345 bp
ATGACTGTCATCGTTGAAAAGCAAATGACCAATGTTGTGATGATTGGTGACGGTCCAACCATGACCAAGATCAGCGGAAGCAAGAGCCACGCGGATGGTGTCGCAACCATGAACACCGCAACCTTTGCTGCACTAGAAAACTATTTTGTTACCAAAATGAACTGGGTTCAAGAATACAGCAGGACACGTAAAGCACCAAGCCGTGGCACTGCGTGTGAATACAAGACCAAATCCTTGTACAAATATGAGTTCTATTCTCAAGCCAACAGTAGAGTTTCAATCCAAACCAAAGTTGAATTAACTCTTATAAACACATTAAGACCTCTTTATACCAATATGAAATAA

Protein Analysis

115

Amino Acids

12.91

Weight (kDa)

9.64

Isoelectric Point (pI)

24.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 3 - 53 5e-11 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0028583)

Species Orthologous Gene IDs
malus_domestica MD02G1295500.v1.1
pyrus_communis pycom15g02510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 308
AccB7I CCANNNNNTGG 1 cut(s) 203
AccII CGCG 1 cut(s) 92
AciI CCGC 3 cut(s) 75, 92, 117
AdeI CACNNNGTG 1 cut(s) 213
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 203
AflIII ACRYGT 1 cut(s) 185
AgsI TTSAA 4 cut(s) 17, 170, 279, 296
ApeKI GCWGC 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 59
AvaII GGWCC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 115
BccI CCATC 1 cut(s) 89
BceAI ACGGC 1 cut(s) 186
BfaI CTAG 1 cut(s) 134
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
Bme18I GGWCC 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 53
BmrI ACTGGG 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 172
BpuEI CTTGAG 1 cut(s) 243
BsaAI YACGTR 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 202
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 167
BseXI GCAGC 1 cut(s) 115
BsgI GTGCAG 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 203
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 1 cut(s) 69
BspACI CCGC 3 cut(s) 75, 92, 117
BspFNI CGCG 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 237
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 1 cut(s) 69
Bst4CI ACNGT 3 cut(s) 7, 53, 269
BstAPI GCANNNNNTGC 1 cut(s) 125
BstAUI TGTACA 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 188
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 1 cut(s) 100
BstFNI CGCG 1 cut(s) 92
BstKTI GATC 1 cut(s) 72
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 1 cut(s) 115
BstXI CCANNNNNNTGG 1 cut(s) 95
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 1 cut(s) 100
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 207
Cfr13I GGNCC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 238
CviAII CATG 2 cut(s) 61, 109
CviJI RGCY 3 cut(s) 87, 201, 263
CviKI_1 RGCY 3 cut(s) 87, 201, 263
CviQI GTAC 1 cut(s) 238
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
DraIII CACNNNGTG 1 cut(s) 213
Eco47I GGWCC 1 cut(s) 53
FaeI CATG 2 cut(s) 64, 112
FaiI YATR 6 cut(s) 62, 110, 246, 308, 330, 338
FatI CATG 2 cut(s) 60, 108
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 1 cut(s) 107
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 1 cut(s) 134
GluI GCNGC 1 cut(s) 129
Hin1II CATG 2 cut(s) 64, 112
HphI GGTGA 1 cut(s) 59
Hpy188III TCNNGA 1 cut(s) 170
HpyCH4III ACNGT 3 cut(s) 7, 53, 269
HpyCH4IV ACGT 1 cut(s) 187
HpyCH4V TGCA 1 cut(s) 131
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpySE526I ACGT 1 cut(s) 187
Hsp92II CATG 2 cut(s) 64, 112
Kzo9I GATC 1 cut(s) 69
LpnPI CCDG 2 cut(s) 148, 166
Lsp1109I GCAGC 1 cut(s) 115
MaeI CTAG 1 cut(s) 134
MaeII ACGT 1 cut(s) 187
MaeIII GTNAC 2 cut(s) 47, 148
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MluCI AATT 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 1 cut(s) 333
MseI TTAA 2 cut(s) 299, 317
MslI CAYNNNNRTG 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 75
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 1 cut(s) 125
NdeII GATC 1 cut(s) 69
NlaIII CATG 2 cut(s) 64, 112
NmuCI GTSAC 1 cut(s) 47
PflMI CCANNNNNTGG 1 cut(s) 203
PkrI GCNGC 1 cut(s) 130
Ppu21I YACGTR 1 cut(s) 188
PsiI TTATAA 1 cut(s) 308
PspPI GGNCC 1 cut(s) 53
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 2 cut(s) 299, 317
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 1 cut(s) 53
SetI ASST 3 cut(s) 125, 190, 325
SinI GGWCC 1 cut(s) 53
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 1 cut(s) 258
SmoI CTYRAG 1 cut(s) 258
Sse9I AATT 1 cut(s) 296
SsiI CCGC 3 cut(s) 75, 92, 117
SspMI CTAG 1 cut(s) 134
TaaI ACNGT 3 cut(s) 7, 53, 269
TaiI ACGT 1 cut(s) 190
TasI AATT 1 cut(s) 296
TatI WGTACW 1 cut(s) 237
Tru1I TTAA 2 cut(s) 299, 317
Tru9I TTAA 2 cut(s) 299, 317
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 2 cut(s) 125, 173
TspRI CASTG 1 cut(s) 214
Van91I CCANNNNNTGG 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 53
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.