pycom15g04270

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0028361
Physical Location & Seq
Forward (+)
2565913 .. 2567109
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g04270.2

Sequence Viewer

Length: 852 bp
ATGAAGTTTAGGAAACCAACCAACCTCATAATTTGCGGTAATCAGCTGATGCAGGTGAAGTCGCAGAACACAACAATCCCAGATGCAGCTGATGCAGCTGTGGACCTGATCAAGAATCTACAAGAAACTGCAGGGTCGAAGTCTTCGATGCAAGCCAACGTTGTAATTGATGATCTTGGAGTTGGAGAATCTTCGTCAATTCTGGAATTCGAGATAGTGAATGTAACGAATGGTGATGCAGCAATAGCAATTGTATTCTGGACACCACGATGTGGACTTGCAAAAGGATTGAATTTAACTCCAAATCGAACAATGATTTCGTCGGTCAAGTTTTTCTTGGGACAGGATTACGATGCTTTAGCCGCAGCCATGACAATTAGGGCAAACAGTTCTTTGTATGTGGATTTCACAATGCCATACACGGAATCAGGTGTGGCGATGGTGGTGCGAACGAAAGATTACACGAGCAAAAATGCATGGGTTTTCTTGAAGCCTCTTTCATGGGATCTCTGGCCGATTTGTCTTTGCTTTTTCATCTGGACTGGTTTCGCGGTTAGTGTTCTTGAACACCGCATTAATGAAGAGTTTCGTGGTCCTCCCTCCGATCAAGTTGGCACAAGATTTTCAGGCCCCATACTCATTGCTGGGGTGGCTTCGTCATTAGCTCTCATCATATCTGCAGCCTCCTTCTTTTACAGGTACGGGTACGTCTTAATGACAAGAGTCGCCTTCGTGTGGAGAAGAATTCGGGACATGTTTGGAACGAAGAATTCGGGTCTTGATGCCATCGAGTGTTTGCCAGCAGCATCTGCTAGCCAAGCCCCTCAAGCCATTCAAATCACGCAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

284

Amino Acids

31.04

Weight (kDa)

7.57

Isoelectric Point (pI)

23.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028361)

Species Orthologous Gene IDs
malus_domestica MD15G1045800.v1.1
pyrus_communis pycom15g04270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 43
AccB7I CCANNNNNTGG 1 cut(s) 272
AccII CGCG 1 cut(s) 551
AciI CCGC 4 cut(s) 36, 363, 551, 571
AclI AACGTT 1 cut(s) 159
AclWI GGATC 1 cut(s) 513
AcoI YGGCCR 1 cut(s) 512
AcsI RAATTY 4 cut(s) 206, 292, 744, 769
AdeI CACNNNGTG 1 cut(s) 272
AfaI GTAC 2 cut(s) 701, 707
AfiI CCNNNNNNNGG 2 cut(s) 272, 735
AflIII ACRYGT 1 cut(s) 753
AgsI TTSAA 4 cut(s) 292, 490, 566, 836
AluBI AGCT 4 cut(s) 46, 89, 98, 665
AluI AGCT 4 cut(s) 46, 89, 98, 665
AlwI GGATC 1 cut(s) 513
AlwNI CAGNNNCTG 1 cut(s) 809
AoxI GGCC 2 cut(s) 512, 628
ApeKI GCWGC 6 cut(s) 86, 95, 239, 365, 680, 803
ApoI RAATTY 4 cut(s) 206, 292, 744, 769
AseI ATTAAT 1 cut(s) 576
Asp700I GAANNNNTTC 1 cut(s) 585
AspS9I GGNCC 3 cut(s) 103, 593, 629
AsuHPI GGTGA 2 cut(s) 67, 245
AsuNHI GCTAGC 1 cut(s) 812
AvaII GGWCC 2 cut(s) 103, 593
BauI CACGAG 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 135
BbvI GCAGC 6 cut(s) 98, 107, 251, 377, 692, 815
BccI CCATC 2 cut(s) 433, 794
BcgI CGANNNNNNTGC 2 cut(s) 427, 461
BclI TGATCA 1 cut(s) 108
BfaI CTAG 2 cut(s) 813, 850
BfmI CTRYAG 2 cut(s) 129, 678
BfuAI ACCTGC 1 cut(s) 43
BglII AGATCT 1 cut(s) 846
BisI GCNGC 7 cut(s) 87, 96, 240, 363, 366, 681, 804
BlsI GCNGC 7 cut(s) 88, 97, 241, 364, 367, 682, 805
Bme18I GGWCC 2 cut(s) 103, 593
BmgT120I GGNCC 3 cut(s) 103, 593, 629
BmiI GGNNCC 1 cut(s) 631
BmsI GCATC 8 cut(s) 39, 73, 82, 138, 226, 343, 772, 815
BmtI GCTAGC 1 cut(s) 816
BoxI GACNNNNGTC 1 cut(s) 722
BpiI GAAGAC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 810
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 735
Bse1I ACTGG 1 cut(s) 547
Bse3DI GCAATG 1 cut(s) 639
BseLI CCNNNNNNNGG 2 cut(s) 272, 735
BseMI GCAATG 1 cut(s) 639
BseNI ACTGG 1 cut(s) 547
BseXI GCAGC 6 cut(s) 98, 107, 251, 377, 692, 815
BseYI CCCAGC 1 cut(s) 644
Bsh1236I CGCG 1 cut(s) 551
BshFI GGCC 2 cut(s) 514, 630
BslFI GGGAC 2 cut(s) 354, 764
BslI CCNNNNNNNGG 2 cut(s) 272, 735
BsmFI GGGAC 2 cut(s) 354, 764
BsnI GGCC 2 cut(s) 514, 630
Bsp143I GATC 5 cut(s) 108, 172, 505, 604, 846
BspACI CCGC 4 cut(s) 36, 363, 551, 571
BspANI GGCC 2 cut(s) 514, 630
BspFNI CGCG 1 cut(s) 551
BspLI GGNNCC 1 cut(s) 631
BspMAI CTGCAG 2 cut(s) 133, 682
BspMI ACCTGC 1 cut(s) 43
BspOI GCTAGC 1 cut(s) 816
BspPI GGATC 1 cut(s) 513
BsrDI GCAATG 1 cut(s) 639
BsrI ACTGG 1 cut(s) 547
BssMI GATC 5 cut(s) 108, 172, 505, 604, 846
BssSI CACGAG 1 cut(s) 463
Bst2BI CACGAG 1 cut(s) 463
Bst4CI ACNGT 1 cut(s) 389
Bst6I CTCTTC 1 cut(s) 576
BstAPI GCANNNNNTGC 2 cut(s) 92, 809
BstC8I GCNNGC 3 cut(s) 153, 801, 814
BstFNI CGCG 1 cut(s) 551
BstKTI GATC 5 cut(s) 111, 175, 508, 607, 849
BstMBI GATC 5 cut(s) 108, 172, 505, 604, 846
BstMWI GCNNNNNNNGC 8 cut(s) 92, 95, 245, 362, 650, 809, 818, 827
BstNSI RCATGY 1 cut(s) 757
BstPAI GACNNNNGTC 1 cut(s) 722
BstSFI CTRYAG 2 cut(s) 129, 678
BstUI CGCG 1 cut(s) 551
BstV1I GCAGC 6 cut(s) 98, 107, 251, 377, 692, 815
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 2 cut(s) 505, 846
BstYI RGATCY 2 cut(s) 505, 846
BsuRI GGCC 2 cut(s) 514, 630
BtgZI GCGATG 1 cut(s) 452
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 3 cut(s) 153, 801, 814
CaiI CAGNNNCTG 1 cut(s) 809
Cfr13I GGNCC 3 cut(s) 103, 593, 629
Csp6I GTAC 2 cut(s) 700, 706
CviAII CATG 4 cut(s) 370, 477, 501, 754
CviQI GTAC 2 cut(s) 700, 706
DpnI GATC 5 cut(s) 110, 174, 507, 606, 848
DpnII GATC 5 cut(s) 108, 172, 505, 604, 846
DraIII CACNNNGTG 1 cut(s) 272
EaeI YGGCCR 1 cut(s) 512
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
Eco47I GGWCC 2 cut(s) 103, 593
EcoO109I RGGNCCY 1 cut(s) 629
EcoRI GAATTC 3 cut(s) 206, 744, 769
EcoT22I ATGCAT 1 cut(s) 478
FaeI CATG 4 cut(s) 373, 480, 504, 757
FaiI YATR 9 cut(s) 29, 371, 399, 418, 478, 502, 635, 674, 755
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 2 cut(s) 354, 764
FatI CATG 4 cut(s) 369, 476, 500, 753
FbaI TGATCA 1 cut(s) 108
Fnu4HI GCNGC 7 cut(s) 87, 96, 240, 363, 366, 681, 804
Fsp4HI GCNGC 7 cut(s) 87, 96, 240, 363, 366, 681, 804
FspBI CTAG 2 cut(s) 813, 850
GluI GCNGC 7 cut(s) 87, 96, 240, 363, 366, 681, 804
GsaI CCCAGC 1 cut(s) 648
HaeIII GGCC 2 cut(s) 514, 630
Hin1II CATG 4 cut(s) 373, 480, 504, 757
HinfI GANTC 4 cut(s) 115, 188, 425, 723
HphI GGTGA 2 cut(s) 67, 245
Hpy166II GTNNAC 2 cut(s) 103, 275
Hpy188I TCNGA 1 cut(s) 604
Hpy188III TCNNGA 9 cut(s) 112, 203, 211, 259, 487, 538, 563, 749, 779
Hpy8I GTNNAC 2 cut(s) 103, 275
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 2 cut(s) 697, 739
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4IV ACGT 2 cut(s) 159, 708
HpyCH4V TGCA 9 cut(s) 52, 86, 95, 131, 151, 239, 281, 476, 680
HpyF10VI GCNNNNNNNGC 8 cut(s) 92, 95, 245, 362, 650, 809, 818, 827
HpySE526I ACGT 2 cut(s) 159, 708
Hsp92II CATG 4 cut(s) 373, 480, 504, 757
Ksp22I TGATCA 1 cut(s) 108
Kzo9I GATC 5 cut(s) 108, 172, 505, 604, 846
Lsp1109I GCAGC 6 cut(s) 98, 107, 251, 377, 692, 815
LweI GCATC 8 cut(s) 39, 73, 82, 138, 226, 343, 772, 815
MaeI CTAG 2 cut(s) 813, 850
MaeII ACGT 2 cut(s) 159, 708
MaeIII GTNAC 1 cut(s) 223
MalI GATC 5 cut(s) 110, 174, 507, 606, 848
MboI GATC 5 cut(s) 108, 172, 505, 604, 846
MboII GAAGA 5 cut(s) 135, 183, 593, 753, 778
MfeI CAATTG 1 cut(s) 249
MflI RGATCY 2 cut(s) 505, 846
MluCI AATT 9 cut(s) 30, 165, 198, 206, 249, 292, 375, 744, 769
MlyI GAGTC 1 cut(s) 732
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 6 cut(s) 35, 504, 606, 610, 694, 834
Mph1103I ATGCAT 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 3 cut(s) 296, 576, 713
MslI CAYNNNNRTG 1 cut(s) 268
MspA1I CMGCKG 3 cut(s) 46, 89, 98
MunI CAATTG 1 cut(s) 249
MvnI CGCG 1 cut(s) 551
MwoI GCNNNNNNNGC 8 cut(s) 92, 95, 245, 362, 650, 809, 818, 827
NdeII GATC 5 cut(s) 108, 172, 505, 604, 846
NheI GCTAGC 1 cut(s) 812
NlaIII CATG 4 cut(s) 373, 480, 504, 757
NlaIV GGNNCC 1 cut(s) 631
NsiI ATGCAT 1 cut(s) 478
NspI RCATGY 1 cut(s) 757
PaqCI CACCTGC 1 cut(s) 43
PciI ACATGT 1 cut(s) 753
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 3 cut(s) 115, 188, 425
PflMI CCANNNNNTGG 1 cut(s) 272
PkrI GCNGC 7 cut(s) 88, 97, 241, 364, 367, 682, 805
PleI GAGTC 1 cut(s) 731
PpsI GAGTC 1 cut(s) 731
PscI ACATGT 1 cut(s) 753
PshAI GACNNNNGTC 1 cut(s) 722
PshBI ATTAAT 1 cut(s) 576
Psp1406I AACGTT 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 644
PspN4I GGNNCC 1 cut(s) 631
PspPI GGNCC 3 cut(s) 103, 593, 629
PstI CTGCAG 2 cut(s) 133, 682
PstNI CAGNNNCTG 1 cut(s) 809
PsuI RGATCY 2 cut(s) 505, 846
PvuII CAGCTG 3 cut(s) 46, 89, 98
RsaI GTAC 2 cut(s) 701, 707
RsaNI GTAC 2 cut(s) 700, 706
RseI CAYNNNNRTG 1 cut(s) 268
SaqAI TTAA 3 cut(s) 296, 576, 713
SatI GCNGC 7 cut(s) 87, 96, 240, 363, 366, 681, 804
Sau3AI GATC 5 cut(s) 108, 172, 505, 604, 846
Sau96I GGNCC 3 cut(s) 103, 593, 629
SchI GAGTC 1 cut(s) 732
SfaNI GCATC 8 cut(s) 39, 73, 82, 138, 226, 343, 772, 815
SfcI CTRYAG 2 cut(s) 129, 678
SinI GGWCC 2 cut(s) 103, 593
SmiMI CAYNNNNRTG 1 cut(s) 268
SmlI CTYRAG 1 cut(s) 825
SmoI CTYRAG 1 cut(s) 825
Sse9I AATT 9 cut(s) 30, 165, 198, 206, 249, 292, 375, 744, 769
SsiI CCGC 4 cut(s) 36, 363, 551, 571
SspMI CTAG 2 cut(s) 813, 850
TaaI ACNGT 1 cut(s) 389
TaiI ACGT 2 cut(s) 162, 711
TaqI TCGA 5 cut(s) 137, 146, 210, 307, 789
TaqII GACCGA 1 cut(s) 313
TasI AATT 9 cut(s) 30, 165, 198, 206, 249, 292, 375, 744, 769
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 3 cut(s) 115, 188, 425
Tru1I TTAA 3 cut(s) 296, 576, 713
Tru9I TTAA 3 cut(s) 296, 576, 713
TseI GCWGC 6 cut(s) 86, 95, 239, 365, 680, 803
TspDTI ATGAA 4 cut(s) 17, 489, 523, 594
TspGWI ACGGA 1 cut(s) 437
Van91I CCANNNNNTGG 1 cut(s) 272
VpaK11BI GGWCC 2 cut(s) 103, 593
VspI ATTAAT 1 cut(s) 576
XapI RAATTY 4 cut(s) 206, 292, 744, 769
XceI RCATGY 1 cut(s) 757
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 2 cut(s) 813, 850
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.