pycom15g05510

Cytochrome b5-like Heme/Steroid binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016766
Physical Location & Seq
Forward (+)
3284709 .. 3286051
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1272 bp
ATGGCGGCAGAGGCAAGATCGATCTACAACGTCTCCGATTGGGCCAACCACCATCCCGGCGGCGAGCTCCCGTTGCTCAGCCTCGCCGGCCAAGACGTCACGGACGCGTTTGTCGCTTACCACCCCGGGTCGGCGTGGCAGCACCTCGACCAGTTCTTCACCGGATTCCAGCTCGAAAGCTATTCTGTTTCCGAAGTTTCCAAAGATTACAGGAAGCTCGTCAACGAGTTTACGAAAATGGGGCTTTTCGAAAACAGGGGACACGGCGTCCATTTCTCTCTGTTTCTGGTGGCATTGCTGAGCGTCTACAGCGTCTTGTGCTCTGATAGCTGTTGGGTTCATCTGGGTTCCGGTGGATTGATGGGTTTTCTCTGGATTCAAAGCGGGTGGCTCGGCCACGATTCTGGTCACTACCAGATCATCAGCAGCAAAAGGGTCAACAGATTCGCCCAGATCCTCACCGGAAACTGCCTTGCCGGCATCAGCATTGCGTGGTGGAAGCGCAACCACAACGCCCACCACATTGCTGTCAACAGCCTCGAACTCGATCCGGATCTCCAGCACATGCCCTTTTTCGCTGTGAATTCAAAACTGTTCACCTCCCTCACCTCTTATTACTACGACAGGAAGATGAGTTTCGATGCTTGTACCAGCTTCTTGGTTAGTTACCAGTACTGGACTTTTTACCCGGTCATGTGTTTGGCTAGGATTAACCTTTTTGCTCAGTCATTTTTACTGTTATCTTCCAAAAGATTGACGGTAAATAATAGAATTGAAGAAATTTTGGGGCTTTTGGTGTTTTGGATTTGGTATGCTCTGCTGGTTTCTTACCTGCCCAATTGGAGGGAGAGAGTAATGTTCGTTGTGGCAAGCTTTTCTGTCACTGGAATCCTACATGTTCAGTTCTGCTTGAACCATTTCTCTTCAAGCGTATACGTTGGTACGCCAACGGCGAACGATTGGTGTGAAAACCAGGCGAATGGTTCACTCAATATAGTCTGCCCGTTGTGGATGGACTGGTTCCACGGCGGATTGCAGCTTCAGATCGAGCACCATATGTTTCCGCTGTTGACTCGGGGCAACGTCAGGAAAGTGGCCCCCCTTATCAAGGCACTCTGTGAGAAGCACAACCTGCCTTTCACAATTGTGTCATTCTACAAGGCCAATGAACTCACCGTTGGGATGCTGCGCACGGCAGTGTTGCAGGCTCGCGATCTAGCTAGCCCGGTTCCGAAGAACCTAGTTTGGGAAGCTGTACACTCTGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5 FA_desaturase

Protein Analysis

424

Amino Acids

48.05

Weight (kDa)

7.31

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 6 - 55 9.3e-13 Cytochrome b5-like Heme/Steroid binding domain
FA_desaturase PF00487 112 - 381 7.8e-30 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016766)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31760
malus_domestica MD08G1063400.v1.1
prunus_persica Prupe.1G411100_v2.0.a1
pyrus_communis pycom08g05210 pycom15g05510
rosa_chinensis RchiOBHm_Chr6g0311061
rosa_laevigata RLG00000010439
rosa_multiflora Rmu_sc0012235.1_g000004
rosa_roxburghii Rroxscaffold_7G00158060
rosa_rugosa Rorug06G0385000
rosa_samantha Rh6AG499000 Rh6BG509600 Rh6CG515400
rosa_wichuraiana Rw6G043460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AatII GACGTC 1 cut(s) 99
Acc16I TGCGCA 1 cut(s) 1190
Acc36I ACCTGC 2 cut(s) 840, 1140
AccI GTMKAC 2 cut(s) 306, 933
AccII CGCG 2 cut(s) 107, 1212
AccIII TCCGGA 1 cut(s) 550
AciI CCGC 5 cut(s) 5, 60, 384, 1029, 1064
AclWI GGATC 3 cut(s) 448, 542, 561
AcoI YGGCCR 2 cut(s) 88, 394
AcsI RAATTY 2 cut(s) 583, 780
AcuI CTGAAG 1 cut(s) 1025
AcyI GRCGYC 2 cut(s) 96, 267
AdeI CACNNNGTG 1 cut(s) 1118
AfaI GTAC 4 cut(s) 649, 674, 943, 1257
AfiI CCNNNNNNNGG 4 cut(s) 130, 843, 1108, 1246
AflIII ACRYGT 2 cut(s) 105, 895
AgsI TTSAA 5 cut(s) 380, 588, 776, 913, 927
AhdI GACNNNNNGTC 1 cut(s) 266
AjnI CCWGG 1 cut(s) 972
AjuI GAANNNNNNNTTGG 2 cut(s) 1161, 1193
AleI CACNNNNGTG 2 cut(s) 1145, 1196
Alw21I GWGCWC 3 cut(s) 69, 323, 1053
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 3 cut(s) 448, 542, 561
Ama87I CYCGRG 2 cut(s) 125, 1074
Aor13HI TCCGGA 1 cut(s) 550
AoxI GGCC 5 cut(s) 42, 88, 394, 1095, 1161
ApeKI GCWGC 4 cut(s) 139, 426, 1036, 1186
ApoI RAATTY 2 cut(s) 583, 780
Asp700I GAANNNNTTC 1 cut(s) 917
AspLEI GCGC 2 cut(s) 504, 1191
AspS9I GGNCC 2 cut(s) 42, 1096
AsuC2I CCSGG 5 cut(s) 57, 126, 127, 689, 1226
AsuHPI GGTGA 5 cut(s) 151, 451, 589, 598, 1165
AsuII TTCGAA 1 cut(s) 249
AsuNHI GCTAGC 1 cut(s) 1220
AvaI CYCGRG 2 cut(s) 125, 1074
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 3 cut(s) 69, 323, 1053
BbvI GCAGC 4 cut(s) 151, 438, 1048, 1173
BccI CCATC 3 cut(s) 60, 355, 1006
BceAI ACGGC 4 cut(s) 280, 966, 1042, 1209
BciT130I CCWGG 1 cut(s) 974
BcnI CCSGG 5 cut(s) 57, 126, 127, 689, 1226
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 4 cut(s) 705, 1217, 1221, 1241
BfmI CTRYAG 1 cut(s) 307
BfuAI ACCTGC 2 cut(s) 840, 1140
BglI GCCNNNNNGGC 2 cut(s) 87, 477
BisI GCNGC 6 cut(s) 6, 61, 140, 427, 1037, 1187
BlpI GCTNAGC 2 cut(s) 77, 299
BlsI GCNGC 6 cut(s) 7, 62, 141, 428, 1038, 1188
BmcAI AGTACT 1 cut(s) 674
Bme1390I CCNGG 6 cut(s) 57, 126, 127, 689, 974, 1226
BmeRI GACNNNNNGTC 1 cut(s) 266
BmeT110I CYCGRG 2 cut(s) 125, 1074
BmgT120I GGNCC 2 cut(s) 42, 1096
BmiI GGNNCC 4 cut(s) 349, 1022, 1098, 1230
BmrFI CCNGG 6 cut(s) 57, 126, 127, 689, 974, 1226
BmsI GCATC 3 cut(s) 489, 631, 1173
BmtI GCTAGC 1 cut(s) 1224
BpmI CTGGAG 1 cut(s) 542
Bpu1102I GCTNAGC 2 cut(s) 77, 299
Bpu14I TTCGAA 1 cut(s) 249
BpuMI CCSGG 5 cut(s) 57, 126, 127, 689, 1226
Bsa29I ATCGAT 1 cut(s) 20
BsaBI GATNNNNATC 1 cut(s) 552
BsaHI GRCGYC 2 cut(s) 96, 267
BsaJI CCNNGG 3 cut(s) 124, 125, 1024
BsaWI WCCGGW 4 cut(s) 161, 350, 461, 550
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 843, 1108, 1246
Bse118I RCCGGY 2 cut(s) 86, 476
Bse1I ACTGG 5 cut(s) 151, 670, 680, 889, 1022
Bse3DI GCAATG 3 cut(s) 293, 486, 522
Bse8I GATNNNNATC 1 cut(s) 552
BseAI TCCGGA 1 cut(s) 550
BseBI CCWGG 1 cut(s) 974
BseCI ATCGAT 1 cut(s) 20
BseDI CCNNGG 3 cut(s) 124, 125, 1024
BseGI GGATG 3 cut(s) 52, 1017, 1188
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 4 cut(s) 130, 843, 1108, 1246
BseMI GCAATG 3 cut(s) 293, 486, 522
BseMII CTCAG 3 cut(s) 91, 290, 737
BseNI ACTGG 5 cut(s) 151, 670, 680, 889, 1022
BseXI GCAGC 4 cut(s) 151, 438, 1048, 1173
Bsh1236I CGCG 2 cut(s) 107, 1212
BshFI GGCC 5 cut(s) 44, 90, 396, 1097, 1163
BshVI ATCGAT 1 cut(s) 20
BsiHKAI GWGCWC 3 cut(s) 69, 323, 1053
BsiHKCI CYCGRG 2 cut(s) 125, 1074
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 4 cut(s) 130, 843, 1108, 1246
BsmAI GTCTC 1 cut(s) 37
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 273
BsnI GGCC 5 cut(s) 44, 90, 396, 1097, 1163
BsoBI CYCGRG 2 cut(s) 125, 1074
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 3 cut(s) 69, 323, 1053
Bsp13I TCCGGA 1 cut(s) 550
Bsp1407I TGTACA 1 cut(s) 1255
Bsp143I GATC 8 cut(s) 17, 21, 417, 453, 547, 553, 1044, 1213
Bsp1720I GCTNAGC 2 cut(s) 77, 299
Bsp68I TCGCGA 1 cut(s) 1212
BspACI CCGC 5 cut(s) 5, 60, 384, 1029, 1064
BspANI GGCC 5 cut(s) 44, 90, 396, 1097, 1163
BspCNI CTCAG 3 cut(s) 90, 291, 736
BspDI ATCGAT 1 cut(s) 20
BspEI TCCGGA 1 cut(s) 550
BspFNI CGCG 2 cut(s) 107, 1212
BspLI GGNNCC 4 cut(s) 349, 1022, 1098, 1230
BspMI ACCTGC 2 cut(s) 840, 1140
BspOI GCTAGC 1 cut(s) 1224
BspPI GGATC 3 cut(s) 448, 542, 561
BspT104I TTCGAA 1 cut(s) 249
BsrDI GCAATG 3 cut(s) 293, 486, 522
BsrFI RCCGGY 2 cut(s) 86, 476
BsrGI TGTACA 1 cut(s) 1255
BsrI ACTGG 5 cut(s) 151, 670, 680, 889, 1022
BssAI RCCGGY 2 cut(s) 86, 476
BssECI CCNNGG 3 cut(s) 124, 125, 1024
BssMI GATC 8 cut(s) 17, 21, 417, 453, 547, 553, 1044, 1213
BssNAI GTATAC 1 cut(s) 934
BssNI GRCGYC 2 cut(s) 96, 267
Bst1107I GTATAC 1 cut(s) 934
Bst2UI CCWGG 1 cut(s) 974
Bst4CI ACNGT 4 cut(s) 594, 738, 760, 1177
Bst6I CTCTTC 1 cut(s) 928
BstACI GRCGYC 2 cut(s) 96, 267
BstAPI GCANNNNNTGC 1 cut(s) 1132
BstAUI TGTACA 1 cut(s) 1255
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 7 cut(s) 65, 88, 478, 871, 1206, 1210, 1222
BstDEI CTNAG 3 cut(s) 77, 299, 723
BstDSI CCRYGG 1 cut(s) 1024
BstENI CCTNNNNNAGG 1 cut(s) 1106
BstF5I GGATG 3 cut(s) 52, 1017, 1188
BstFNI CGCG 2 cut(s) 107, 1212
BstHHI GCGC 2 cut(s) 504, 1191
BstKTI GATC 8 cut(s) 20, 24, 420, 456, 550, 556, 1047, 1216
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 8 cut(s) 17, 21, 417, 453, 547, 553, 1044, 1213
BstMWI GCNNNNNNNGC 9 cut(s) 11, 73, 87, 113, 309, 318, 327, 477, 1132
BstNI CCWGG 1 cut(s) 974
BstNSI RCATGY 2 cut(s) 568, 899
BstSCI CCNGG 6 cut(s) 55, 124, 125, 687, 972, 1224
BstSFI CTRYAG 1 cut(s) 307
BstUI CGCG 2 cut(s) 107, 1212
BstV1I GCAGC 4 cut(s) 151, 438, 1048, 1173
BstX2I RGATCY 2 cut(s) 453, 553
BstXI CCANNNNNNTGG 3 cut(s) 404, 658, 980
BstYI RGATCY 2 cut(s) 453, 553
BstZ17I GTATAC 1 cut(s) 934
Bsu15I ATCGAT 1 cut(s) 20
BsuRI GGCC 5 cut(s) 44, 90, 396, 1097, 1163
BsuTUI ATCGAT 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 1024
BtsCI GGATG 3 cut(s) 52, 1017, 1188
BtsI GCAGTG 1 cut(s) 1203
BtsIMutI CAGTG 2 cut(s) 882, 1203
BtuMI TCGCGA 1 cut(s) 1212
BveI ACCTGC 2 cut(s) 840, 1140
Cac8I GCNNGC 7 cut(s) 65, 88, 478, 871, 1206, 1210, 1222
CfoI GCGC 2 cut(s) 504, 1191
Cfr10I RCCGGY 2 cut(s) 86, 476
Cfr13I GGNCC 2 cut(s) 42, 1096
Cfr9I CCCGGG 1 cut(s) 125
ClaI ATCGAT 1 cut(s) 20
CseI GACGC 4 cut(s) 113, 256, 292, 301
Csp6I GTAC 4 cut(s) 648, 673, 942, 1256
CspCI CAANNNNNGTGG 2 cut(s) 368, 403
CviAII CATG 3 cut(s) 565, 694, 896
CviQI GTAC 4 cut(s) 648, 673, 942, 1256
DdeI CTNAG 3 cut(s) 77, 299, 723
DpnI GATC 8 cut(s) 19, 23, 419, 455, 549, 555, 1046, 1215
DpnII GATC 8 cut(s) 17, 21, 417, 453, 547, 553, 1044, 1213
DraIII CACNNNGTG 1 cut(s) 1118
DrdI GACNNNNNNGTC 1 cut(s) 110
DriI GACNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 2 cut(s) 88, 394
Eam1104I CTCTTC 1 cut(s) 928
Eam1105I GACNNNNNGTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 928
EciI GGCGGA 1 cut(s) 1044
Ecl136II GAGCTC 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 69
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 1025
Eco88I CYCGRG 2 cut(s) 125, 1074
EcoICRI GAGCTC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 1106
EcoRI GAATTC 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 972
EcoT38I GRGCYC 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 3 cut(s) 568, 697, 899
FaiI YATR 8 cut(s) 566, 695, 813, 897, 934, 995, 1056, 1058
FaqI GGGAC 1 cut(s) 273
FatI CATG 3 cut(s) 564, 693, 895
FauI CCCGC 1 cut(s) 377
FauNDI CATATG 1 cut(s) 1056
FblI GTMKAC 2 cut(s) 306, 933
Fnu4HI GCNGC 6 cut(s) 6, 61, 140, 427, 1037, 1187
FokI GGATG 3 cut(s) 39, 1024, 1195
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 6 cut(s) 6, 61, 140, 427, 1037, 1187
FspBI CTAG 4 cut(s) 705, 1217, 1221, 1241
FspI TGCGCA 1 cut(s) 1190
GlaI GCGC 2 cut(s) 503, 1190
GluI GCNGC 6 cut(s) 6, 61, 140, 427, 1037, 1187
GsuI CTGGAG 1 cut(s) 542
HaeIII GGCC 5 cut(s) 44, 90, 396, 1097, 1163
HgaI GACGC 4 cut(s) 113, 256, 292, 301
HhaI GCGC 2 cut(s) 504, 1191
Hin1I GRCGYC 2 cut(s) 96, 267
Hin1II CATG 3 cut(s) 568, 697, 899
Hin6I GCGC 2 cut(s) 502, 1189
HinP1I GCGC 2 cut(s) 502, 1189
HincII GTYRAC 4 cut(s) 223, 439, 532, 1071
HindII GTYRAC 4 cut(s) 223, 439, 532, 1071
HindIII AAGCTT 1 cut(s) 871
HinfI GANTC 6 cut(s) 165, 376, 401, 444, 888, 1072
HphI GGTGA 5 cut(s) 151, 451, 589, 598, 1165
Hpy188I TCNGA 5 cut(s) 37, 193, 325, 1044, 1233
Hpy188III TCNNGA 4 cut(s) 373, 551, 1087, 1211
HpyCH4III ACNGT 4 cut(s) 594, 738, 760, 1177
HpyCH4IV ACGT 4 cut(s) 30, 96, 936, 1083
HpyCH4V TGCA 2 cut(s) 1036, 1204
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 73, 87, 113, 309, 318, 327, 477, 1132
HpyF3I CTNAG 3 cut(s) 77, 299, 723
HpySE526I ACGT 4 cut(s) 30, 96, 936, 1083
Hsp92I GRCGYC 2 cut(s) 96, 267
Hsp92II CATG 3 cut(s) 568, 697, 899
HspAI GCGC 2 cut(s) 502, 1189
Kpn2I TCCGGA 1 cut(s) 550
KroI GCCGGC 2 cut(s) 86, 476
KroNI GCCGGC 2 cut(s) 88, 478
Kzo9I GATC 8 cut(s) 17, 21, 417, 453, 547, 553, 1044, 1213
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 4 cut(s) 151, 438, 1048, 1173
LweI GCATC 3 cut(s) 489, 631, 1173
MaeI CTAG 4 cut(s) 705, 1217, 1221, 1241
MaeII ACGT 4 cut(s) 30, 96, 936, 1083
MaeIII GTNAC 4 cut(s) 97, 407, 665, 880
MalI GATC 8 cut(s) 19, 23, 419, 455, 549, 555, 1046, 1215
MboI GATC 8 cut(s) 17, 21, 417, 453, 547, 553, 1044, 1213
MboII GAAGA 6 cut(s) 148, 640, 735, 788, 915, 1246
MfeI CAATTG 2 cut(s) 838, 1143
MflI RGATCY 2 cut(s) 453, 553
MhlI GDGCHC 3 cut(s) 69, 323, 1053
MluCI AATT 5 cut(s) 583, 771, 780, 838, 1143
MluI ACGCGT 1 cut(s) 105
MlyI GAGTC 1 cut(s) 1066
MmeI TCCRAC 1 cut(s) 1246
MnlI CCTC 9 cut(s) 4, 92, 155, 467, 548, 610, 614, 619, 837
MroI TCCGGA 1 cut(s) 550
MroNI GCCGGC 2 cut(s) 86, 476
MroXI GAANNNNTTC 1 cut(s) 917
MseI TTAA 1 cut(s) 711
MslI CAYNNNNRTG 2 cut(s) 1145, 1196
MspA1I CMGCKG 1 cut(s) 1066
MspR9I CCNGG 6 cut(s) 57, 126, 127, 689, 974, 1226
MunI CAATTG 2 cut(s) 838, 1143
MvaI CCWGG 1 cut(s) 974
MvnI CGCG 2 cut(s) 107, 1212
MwoI GCNNNNNNNGC 9 cut(s) 11, 73, 87, 113, 309, 318, 327, 477, 1132
NaeI GCCGGC 2 cut(s) 88, 478
NciI CCSGG 5 cut(s) 57, 126, 127, 689, 1226
NdeI CATATG 1 cut(s) 1056
NdeII GATC 8 cut(s) 17, 21, 417, 453, 547, 553, 1044, 1213
NgoMIV GCCGGC 2 cut(s) 86, 476
NheI GCTAGC 1 cut(s) 1220
NlaIII CATG 3 cut(s) 568, 697, 899
NlaIV GGNNCC 4 cut(s) 349, 1022, 1098, 1230
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 3 cut(s) 97, 407, 880
NruI TCGCGA 1 cut(s) 1212
NsbI TGCGCA 1 cut(s) 1190
NspI RCATGY 2 cut(s) 568, 899
NspV TTCGAA 1 cut(s) 249
OliI CACNNNNGTG 2 cut(s) 1145, 1196
PciI ACATGT 1 cut(s) 895
PcsI WCGNNNNNNNCGW 1 cut(s) 950
PdiI GCCGGC 2 cut(s) 88, 478
PdmI GAANNNNTTC 1 cut(s) 917
PfeI GAWTC 5 cut(s) 165, 376, 401, 444, 888
PkrI GCNGC 6 cut(s) 7, 62, 141, 428, 1038, 1188
PleI GAGTC 1 cut(s) 1066
PpsI GAGTC 1 cut(s) 1066
PscI ACATGT 1 cut(s) 895
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 972
PspGI CCWGG 1 cut(s) 972
PspN4I GGNNCC 4 cut(s) 349, 1022, 1098, 1230
PspPI GGNCC 2 cut(s) 42, 1096
PsuI RGATCY 2 cut(s) 453, 553
RruI TCGCGA 1 cut(s) 1212
RsaI GTAC 4 cut(s) 649, 674, 943, 1257
RsaNI GTAC 4 cut(s) 648, 673, 942, 1256
RseI CAYNNNNRTG 2 cut(s) 1145, 1196
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 711
SatI GCNGC 6 cut(s) 6, 61, 140, 427, 1037, 1187
Sau3AI GATC 8 cut(s) 17, 21, 417, 453, 547, 553, 1044, 1213
Sau96I GGNCC 2 cut(s) 42, 1096
ScaI AGTACT 1 cut(s) 674
SchI GAGTC 1 cut(s) 1066
ScrFI CCNGG 6 cut(s) 57, 126, 127, 689, 974, 1226
SduI GDGCHC 3 cut(s) 69, 323, 1053
SfaNI GCATC 3 cut(s) 489, 631, 1173
SfcI CTRYAG 1 cut(s) 307
SfuI TTCGAA 1 cut(s) 249
SmaI CCCGGG 1 cut(s) 127
SmiMI CAYNNNNRTG 2 cut(s) 1145, 1196
Sse9I AATT 5 cut(s) 583, 771, 780, 838, 1143
SsiI CCGC 5 cut(s) 5, 60, 384, 1029, 1064
SspMI CTAG 4 cut(s) 705, 1217, 1221, 1241
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 6 cut(s) 55, 124, 125, 687, 972, 1224
TaaI ACNGT 4 cut(s) 594, 738, 760, 1177
TaiI ACGT 4 cut(s) 33, 99, 939, 1086
TaqI TCGA 8 cut(s) 20, 147, 174, 249, 540, 546, 639, 1047
TasI AATT 5 cut(s) 583, 771, 780, 838, 1143
TatI WGTACW 2 cut(s) 672, 1255
TauI GCSGC 2 cut(s) 8, 63
TfiI GAWTC 5 cut(s) 165, 376, 401, 444, 888
Tru1I TTAA 1 cut(s) 711
Tru9I TTAA 1 cut(s) 711
TscAI CASTG 2 cut(s) 889, 1203
TseFI GTSAC 3 cut(s) 97, 407, 880
TseI GCWGC 4 cut(s) 139, 426, 1036, 1186
Tsp45I GTSAC 3 cut(s) 97, 407, 880
TspDTI ATGAA 2 cut(s) 329, 1182
TspGWI ACGGA 1 cut(s) 116
TspMI CCCGGG 1 cut(s) 125
TspRI CASTG 2 cut(s) 889, 1203
XagI CCTNNNNNAGG 1 cut(s) 1106
XapI RAATTY 2 cut(s) 583, 780
XceI RCATGY 2 cut(s) 568, 899
XmaI CCCGGG 1 cut(s) 125
XmiI GTMKAC 2 cut(s) 306, 933
XmnI GAANNNNTTC 1 cut(s) 917
XspI CTAG 4 cut(s) 705, 1217, 1221, 1241
ZraI GACGTC 1 cut(s) 97
ZrmI AGTACT 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.