pycom15g07760

substituted 4 bases at 4 genomic stop codons

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0022388
Physical Location & Seq
Forward (+)
4806642 .. 4808629
1988 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 720 bp
ATGAACCCACAAATCGCTTTCATGAGGGGGGCAATGAAGATCAAGGGCAGCCTGAGTGCGGCCACGAAATTCACACCAGACATTTTCCCCAAGCCTGCCAAGATGGGTTCAAGTAAAAAAGGAGGGTGGTACAACTCACTCAAGACCTCCATTCTTGAACTTGCTGACTCTGGCATCACCCATGTCTGGCTTCCTCCGCCTCACACTAATGGAACCGAGGGATGCTCTCCGGAAGGCTATACGATCTCGATGCATCGAGAATTGAAGTTCCTATTTACTGCCAGGCATGAGAAGGGAATCAAAGCCATAGCAGACGTTGTGATAAACCACAGAGCAAATGTCAAACAAGATGAGAACGGACTATGGAGCATATTTGAAGGTGGAAAGCCGGATGGACGTCTTGACTTCAAGGCCTCCTTGATTTGCAGGGATGACAATGACCACCCTTTTGGCACTGGCAACCCAGACACAGGAGACAACTTCCCGTTCACAGCTGACATGGACCACACAAACCCTAGGCTTCAAAGCGAGTTGTCTGATTGGATGATCTGGTTGAAAACTGAAATAGGGTTTGATGGATGGAGGTTTGATTATGCGATTGGGTACGGCTCTAGCTTAACCAAGCTCTACATGGACAGAACTTTGCCGGAATTCGCAATTGCCGAGTACTGGCGTTGGATTCAGAAACAAGATGCACATAGGAAATTAGGAATGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase PPR PPR_2

Protein Analysis

240

Amino Acids

27.02

Weight (kDa)

7.79

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022388)

Species Orthologous Gene IDs
malus_domestica MD08G1101700.v1.1
prunus_persica Prupe.1G435600_v2.0.a1
pyrus_communis pycom08g08220 pycom15g07760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 400
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 2 cut(s) 59, 197
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 2 cut(s) 68, 650
AcyI GRCGYC 1 cut(s) 397
AfaI GTAC 3 cut(s) 131, 605, 668
AfiI CCNNNNNNNGG 4 cut(s) 58, 186, 470, 669
AgsI TTSAA 7 cut(s) 111, 158, 265, 377, 409, 524, 556
AjnI CCWGG 1 cut(s) 281
AluBI AGCT 3 cut(s) 494, 615, 625
AluI AGCT 3 cut(s) 494, 615, 625
Alw26I GTCTC 1 cut(s) 468
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 2 cut(s) 60, 411
ApeKI GCWGC 1 cut(s) 48
ApoI RAATTY 2 cut(s) 68, 650
ArsI GACNNNNNNTTYG 2 cut(s) 431, 463
AspA2I CCTAGG 1 cut(s) 515
AspS9I GGNCC 1 cut(s) 502
AsuHPI GGTGA 1 cut(s) 169
AvaII GGWCC 1 cut(s) 502
AvrII CCTAGG 1 cut(s) 515
BbvI GCAGC 1 cut(s) 60
BccI CCATC 4 cut(s) 97, 386, 569, 573
BceAI ACGGC 1 cut(s) 622
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 1 cut(s) 283
BcoDI GTCTC 1 cut(s) 468
BfaI CTAG 2 cut(s) 516, 612
BisI GCNGC 2 cut(s) 49, 60
BlnI CCTAGG 1 cut(s) 515
BlsI GCNGC 2 cut(s) 50, 61
BmcAI AGTACT 1 cut(s) 668
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 1 cut(s) 502
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 283
BmsI GCATC 5 cut(s) 183, 212, 240, 262, 682
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpuEI CTTGAG 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 397
BsaJI CCNNGG 2 cut(s) 216, 515
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 186, 470, 669
Bse1I ACTGG 2 cut(s) 460, 674
Bse3DI GCAATG 1 cut(s) 39
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 2 cut(s) 216, 515
BseGI GGATG 5 cut(s) 227, 397, 436, 549, 584
BseLI CCNNNNNNNGG 4 cut(s) 58, 186, 470, 669
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 2 cut(s) 460, 674
BseXI GCAGC 1 cut(s) 60
BshFI GGCC 2 cut(s) 62, 413
BsiSI CCGG 3 cut(s) 230, 389, 647
BslI CCNNNNNNNGG 4 cut(s) 58, 186, 470, 669
BsmAI GTCTC 1 cut(s) 468
BsnI GGCC 2 cut(s) 62, 413
Bsp13I TCCGGA 1 cut(s) 229
Bsp143I GATC 3 cut(s) 39, 243, 546
BspACI CCGC 2 cut(s) 59, 197
BspANI GGCC 2 cut(s) 62, 413
BspCNI CTCAG 1 cut(s) 45
BspEI TCCGGA 1 cut(s) 229
BspHI TCATGA 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 2 cut(s) 460, 674
BssECI CCNNGG 2 cut(s) 216, 515
BssMI GATC 3 cut(s) 39, 243, 546
BssNI GRCGYC 1 cut(s) 397
BssT1I CCWWGG 1 cut(s) 515
Bst2UI CCWGG 1 cut(s) 283
BstACI GRCGYC 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 1 cut(s) 53
BstF5I GGATG 5 cut(s) 227, 397, 436, 549, 584
BstKTI GATC 3 cut(s) 42, 246, 549
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 3 cut(s) 39, 243, 546
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 60
BstXI CCANNNNNNTGG 1 cut(s) 449
BsuRI GGCC 2 cut(s) 62, 413
BtsCI GGATG 5 cut(s) 227, 397, 436, 549, 584
BtsIMutI CAGTG 1 cut(s) 453
Cac8I GCNNGC 1 cut(s) 96
CciI TCATGA 1 cut(s) 21
Cfr13I GGNCC 1 cut(s) 502
Csp6I GTAC 3 cut(s) 130, 604, 667
CviAII CATG 5 cut(s) 22, 182, 287, 499, 631
CviQI GTAC 3 cut(s) 130, 604, 667
DdeI CTNAG 1 cut(s) 53
DpnI GATC 3 cut(s) 41, 245, 548
DpnII GATC 3 cut(s) 39, 243, 546
EaeI YGGCCR 1 cut(s) 60
EciI GGCGGA 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 515
Eco147I AGGCCT 1 cut(s) 413
Eco47I GGWCC 1 cut(s) 502
EcoRI GAATTC 1 cut(s) 650
EcoRII CCWGG 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 515
EcoT22I ATGCAT 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 515
FaeI CATG 5 cut(s) 25, 185, 290, 502, 634
FalI AAGNNNNNCTT 2 cut(s) 401, 433
FatI CATG 5 cut(s) 21, 181, 286, 498, 630
Fnu4HI GCNGC 2 cut(s) 49, 60
FokI GGATG 5 cut(s) 234, 404, 443, 556, 591
Fsp4HI GCNGC 2 cut(s) 49, 60
FspBI CTAG 2 cut(s) 516, 612
GluI GCNGC 2 cut(s) 49, 60
HaeIII GGCC 2 cut(s) 62, 413
HapII CCGG 3 cut(s) 230, 389, 647
Hin1I GRCGYC 1 cut(s) 397
Hin1II CATG 5 cut(s) 25, 185, 290, 502, 634
HinfI GANTC 3 cut(s) 167, 297, 679
HpaII CCGG 3 cut(s) 230, 389, 647
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 489
Hpy188I TCNGA 2 cut(s) 538, 684
Hpy188III TCNNGA 7 cut(s) 22, 142, 155, 230, 247, 257, 401
Hpy8I GTNNAC 1 cut(s) 489
HpyAV CCTTC 3 cut(s) 227, 286, 371
HpyCH4IV ACGT 2 cut(s) 315, 397
HpyCH4V TGCA 3 cut(s) 253, 426, 695
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 2 cut(s) 315, 397
Hsp92I GRCGYC 1 cut(s) 397
Hsp92II CATG 5 cut(s) 25, 185, 290, 502, 634
Kpn2I TCCGGA 1 cut(s) 229
Kzo9I GATC 3 cut(s) 39, 243, 546
LmnI GCTCC 1 cut(s) 366
Lsp1109I GCAGC 1 cut(s) 60
LweI GCATC 5 cut(s) 183, 212, 240, 262, 682
MaeI CTAG 2 cut(s) 516, 612
MaeII ACGT 2 cut(s) 315, 397
MalI GATC 3 cut(s) 41, 245, 548
MboI GATC 3 cut(s) 39, 243, 546
MboII GAAGA 1 cut(s) 49
MfeI CAATTG 1 cut(s) 657
MluCI AATT 5 cut(s) 68, 260, 650, 657, 704
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 656
MnlI CCTC 8 cut(s) 18, 116, 157, 204, 210, 211, 424, 576
Mph1103I ATGCAT 1 cut(s) 255
MroI TCCGGA 1 cut(s) 229
MseI TTAA 1 cut(s) 617
MslI CAYNNNNRTG 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 3 cut(s) 230, 389, 647
MspR9I CCNGG 1 cut(s) 283
MunI CAATTG 1 cut(s) 657
MvaI CCWGG 1 cut(s) 283
MwoI GCNNNNNNNGC 1 cut(s) 196
NdeII GATC 3 cut(s) 39, 243, 546
NlaIII CATG 5 cut(s) 25, 185, 290, 502, 634
NlaIV GGNNCC 1 cut(s) 214
NmeAIII GCCGAG 1 cut(s) 688
NsiI ATGCAT 1 cut(s) 255
PagI TCATGA 1 cut(s) 21
PceI AGGCCT 1 cut(s) 413
PcsI WCGNNNNNNNCGW 1 cut(s) 660
PfeI GAWTC 2 cut(s) 297, 679
PkrI GCNGC 2 cut(s) 50, 61
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 214
PspPI GGNCC 1 cut(s) 502
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 3 cut(s) 131, 605, 668
RsaNI GTAC 3 cut(s) 130, 604, 667
RseI CAYNNNNRTG 1 cut(s) 207
SaqAI TTAA 1 cut(s) 617
SatI GCNGC 2 cut(s) 49, 60
Sau3AI GATC 3 cut(s) 39, 243, 546
Sau96I GGNCC 1 cut(s) 502
ScaI AGTACT 1 cut(s) 668
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 283
SetI ASST 8 cut(s) 149, 318, 382, 400, 496, 587, 617, 627
SfaNI GCATC 5 cut(s) 183, 212, 240, 262, 682
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 5 cut(s) 68, 260, 650, 657, 704
SseBI AGGCCT 1 cut(s) 413
SsiI CCGC 2 cut(s) 59, 197
SspMI CTAG 2 cut(s) 516, 612
StuI AGGCCT 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 1 cut(s) 515
TaiI ACGT 2 cut(s) 318, 400
TaqI TCGA 2 cut(s) 248, 256
TasI AATT 5 cut(s) 68, 260, 650, 657, 704
TatI WGTACW 1 cut(s) 666
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 2 cut(s) 297, 679
Tru1I TTAA 1 cut(s) 617
Tru9I TTAA 1 cut(s) 617
TscAI CASTG 1 cut(s) 460
TseI GCWGC 1 cut(s) 48
TspDTI ATGAA 3 cut(s) 10, 17, 50
TspGWI ACGGA 1 cut(s) 372
TspRI CASTG 1 cut(s) 460
VpaK11BI GGWCC 1 cut(s) 502
XapI RAATTY 2 cut(s) 68, 650
XcmI CCANNNNNNNNNTGG 1 cut(s) 628
XmaJI CCTAGG 1 cut(s) 515
XspI CTAG 2 cut(s) 516, 612
ZraI GACGTC 1 cut(s) 398
ZrmI AGTACT 1 cut(s) 668
Zsp2I ATGCAT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.