pycom15g08540

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0024287
Physical Location & Seq
Forward (+)
5366238 .. 5366935
698 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGAGCCCTTGCACTGTTTTGGGCTTGAGTTTAGAACAAAAAATTTGTAGTGACCGCATTATTAGAATCAAAATCGAACTCAAAACCTTCGGCTCACTGGGGAAGGTGAATTATATATATATTAAAAAAAGGAAGATTTGCGACCAAGGCACTGAGCAGTTCCCACATCACTTCCATTGCCCAACCCTAGCATCTCTTTGTTTATTTGTCATGGGAGAAAACCAGTCACCATTAAAATGGAAAGGCAAAACCTCTAGAGAGCTCAAAGGCCATGCAGCAGAACAAGTTTGGCCGCTCTTGGCTGATTTTTGTAACTTGCACAAATGGTTGCCGGTCCTTGACGCTTGTTACCTAGTTGACGGAGTCCCCGGCCAGCCAGGTGTGACCCGGTACTGCGTCGCTCCTCTCCCATATCCAGACGACGGGACCATCAAGTGGGCCAAAGAAAAACTCCTCATGATTGATCCAATCAACCATTGTTTAAGCTATGATCGATCATTGAAAACAATTTCGGGTTCAAGTCGTACGTTGCAATCATGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

182

Amino Acids

20.41

Weight (kDa)

8.9

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 84 - 171 4.8e-11 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024287)

Species Orthologous Gene IDs
malus_domestica MD15G1091400.v1.1 MD15G1091500.v1.1
pyrus_communis pycom15g08540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 435
AccBSI CCGCTC 1 cut(s) 295
AciI CCGC 2 cut(s) 55, 293
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 2 cut(s) 290, 370
AcsI RAATTY 1 cut(s) 42
AfaI GTAC 2 cut(s) 392, 526
AfiI CCNNNNNNNGG 2 cut(s) 422, 435
AgsI TTSAA 2 cut(s) 502, 519
AjnI CCWGG 1 cut(s) 376
AluBI AGCT 2 cut(s) 262, 486
AluI AGCT 2 cut(s) 262, 486
Alw21I GWGCWC 1 cut(s) 264
AlwI GGATC 1 cut(s) 458
AoxI GGCC 4 cut(s) 268, 290, 370, 438
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 42
AspS9I GGNCC 3 cut(s) 334, 426, 438
AsuC2I CCSGG 2 cut(s) 369, 388
AsuHPI GGTGA 2 cut(s) 118, 219
AvaII GGWCC 2 cut(s) 334, 426
BanII GRGCYC 2 cut(s) 8, 264
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 1 cut(s) 287
BccI CCATC 1 cut(s) 437
BciT130I CCWGG 1 cut(s) 378
BcnI CCSGG 2 cut(s) 369, 388
BfaI CTAG 3 cut(s) 188, 255, 353
BisI GCNGC 2 cut(s) 276, 293
BlsI GCNGC 2 cut(s) 277, 294
Bme1390I CCNGG 3 cut(s) 369, 378, 388
Bme18I GGWCC 2 cut(s) 334, 426
BmgT120I GGNCC 3 cut(s) 334, 426, 438
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 3 cut(s) 369, 378, 388
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 1 cut(s) 200
BmuI ACTGGG 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 46
BpuMI CCSGG 2 cut(s) 369, 388
Bsa29I ATCGAT 1 cut(s) 493
BsaJI CCNNGG 2 cut(s) 145, 367
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 435
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 2 cut(s) 102, 223
Bse3DI GCAATG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 378
BseCI ATCGAT 1 cut(s) 493
BseDI CCNNGG 2 cut(s) 145, 367
BseLI CCNNNNNNNGG 2 cut(s) 422, 435
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 2 cut(s) 102, 223
BseRI GAGGAG 2 cut(s) 393, 443
BseXI GCAGC 1 cut(s) 287
BshFI GGCC 4 cut(s) 270, 292, 372, 440
BshVI ATCGAT 1 cut(s) 493
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 3 cut(s) 332, 369, 388
BsiWI CGTACG 1 cut(s) 524
BslFI GGGAC 2 cut(s) 350, 439
BslI CCNNNNNNNGG 2 cut(s) 422, 435
BsmFI GGGAC 2 cut(s) 350, 439
BsnI GGCC 4 cut(s) 270, 292, 372, 440
Bsp1286I GDGCHC 2 cut(s) 8, 264
Bsp143I GATC 3 cut(s) 463, 490, 494
BspACI CCGC 2 cut(s) 55, 293
BspANI GGCC 4 cut(s) 270, 292, 372, 440
BspCNI CTCAG 1 cut(s) 145
BspDI ATCGAT 1 cut(s) 493
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 1 cut(s) 427
BspPI GGATC 1 cut(s) 458
BsrBI CCGCTC 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 175
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 2 cut(s) 102, 223
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 2 cut(s) 145, 367
BssMI GATC 3 cut(s) 463, 490, 494
BssT1I CCWWGG 1 cut(s) 145
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 374
BstDEI CTNAG 1 cut(s) 153
BstKTI GATC 3 cut(s) 466, 493, 497
BstMBI GATC 3 cut(s) 463, 490, 494
BstMWI GCNNNNNNNGC 1 cut(s) 147
BstNI CCWGG 1 cut(s) 378
BstSCI CCNGG 3 cut(s) 367, 376, 386
BstV1I GCAGC 1 cut(s) 287
BstXI CCANNNNNNTGG 1 cut(s) 237
Bsu15I ATCGAT 1 cut(s) 493
BsuRI GGCC 4 cut(s) 270, 292, 372, 440
BsuTUI ATCGAT 1 cut(s) 493
BtsIMutI CAGTG 3 cut(s) 12, 95, 150
Cac8I GCNNGC 1 cut(s) 374
CciI TCATGA 1 cut(s) 456
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 3 cut(s) 334, 426, 438
ClaI ATCGAT 1 cut(s) 493
CseI GACGC 2 cut(s) 350, 385
Csp6I GTAC 2 cut(s) 391, 525
CviAII CATG 4 cut(s) 211, 272, 457, 537
CviQI GTAC 2 cut(s) 391, 525
DdeI CTNAG 1 cut(s) 153
DpnI GATC 3 cut(s) 465, 492, 496
DpnII GATC 3 cut(s) 463, 490, 494
EaeI YGGCCR 2 cut(s) 290, 370
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 145
Eco24I GRGCYC 2 cut(s) 8, 264
Eco47I GGWCC 2 cut(s) 334, 426
Eco53kI GAGCTC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 376
EcoT14I CCWWGG 1 cut(s) 145
EcoT38I GRGCYC 2 cut(s) 8, 264
ErhI CCWWGG 1 cut(s) 145
FaeI CATG 4 cut(s) 214, 275, 460, 540
FaqI GGGAC 2 cut(s) 350, 439
FatI CATG 4 cut(s) 210, 271, 456, 536
Fnu4HI GCNGC 2 cut(s) 276, 293
FriOI GRGCYC 2 cut(s) 8, 264
Fsp4HI GCNGC 2 cut(s) 276, 293
FspBI CTAG 3 cut(s) 188, 255, 353
GluI GCNGC 2 cut(s) 276, 293
HaeIII GGCC 4 cut(s) 270, 292, 372, 440
HapII CCGG 3 cut(s) 332, 369, 388
HgaI GACGC 2 cut(s) 350, 385
Hin1II CATG 4 cut(s) 214, 275, 460, 540
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 2 cut(s) 66, 363
HpaII CCGG 3 cut(s) 332, 369, 388
HphI GGTGA 2 cut(s) 118, 219
Hpy166II GTNNAC 1 cut(s) 358
Hpy188III TCNNGA 3 cut(s) 255, 416, 457
Hpy8I GTNNAC 1 cut(s) 358
Hpy99I CGWCG 2 cut(s) 401, 425
HpyAV CCTTC 2 cut(s) 97, 97
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4IV ACGT 1 cut(s) 527
HpyCH4V TGCA 5 cut(s) 12, 275, 319, 532, 540
HpyF10VI GCNNNNNNNGC 1 cut(s) 147
HpyF3I CTNAG 1 cut(s) 153
HpySE526I ACGT 1 cut(s) 527
Hsp92II CATG 4 cut(s) 214, 275, 460, 540
Kzo9I GATC 3 cut(s) 463, 490, 494
LmnI GCTCC 1 cut(s) 406
LpnPI CCDG 9 cut(s) 83, 236, 345, 363, 382, 386, 390, 401, 429
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 1 cut(s) 200
MaeI CTAG 3 cut(s) 188, 255, 353
MaeII ACGT 1 cut(s) 527
MaeIII GTNAC 5 cut(s) 50, 225, 311, 347, 382
MalI GATC 3 cut(s) 465, 492, 496
MbiI CCGCTC 1 cut(s) 295
MboI GATC 3 cut(s) 463, 490, 494
MboII GAAGA 1 cut(s) 145
MhlI GDGCHC 2 cut(s) 8, 264
MluCI AATT 4 cut(s) 42, 109, 507, 541
MlyI GAGTC 1 cut(s) 372
MnlI CCTC 3 cut(s) 262, 414, 464
MseI TTAA 3 cut(s) 123, 233, 482
MslI CAYNNNNRTG 1 cut(s) 235
MspI CCGG 3 cut(s) 332, 369, 388
MspR9I CCNGG 3 cut(s) 369, 378, 388
MvaI CCWGG 1 cut(s) 378
MwoI GCNNNNNNNGC 1 cut(s) 147
NciI CCSGG 2 cut(s) 369, 388
NdeII GATC 3 cut(s) 463, 490, 494
NlaIII CATG 4 cut(s) 214, 275, 460, 540
NlaIV GGNNCC 1 cut(s) 427
NmuCI GTSAC 3 cut(s) 50, 225, 382
PagI TCATGA 1 cut(s) 456
PfeI GAWTC 1 cut(s) 66
Pfl23II CGTACG 1 cut(s) 524
PflFI GACNNNGTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 435
PkrI GCNGC 2 cut(s) 277, 294
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspLI CGTACG 1 cut(s) 524
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 3 cut(s) 334, 426, 438
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 2 cut(s) 392, 526
RsaNI GTAC 2 cut(s) 391, 525
RseI CAYNNNNRTG 1 cut(s) 235
SacI GAGCTC 1 cut(s) 264
SaqAI TTAA 3 cut(s) 123, 233, 482
SatI GCNGC 2 cut(s) 276, 293
Sau3AI GATC 3 cut(s) 463, 490, 494
Sau96I GGNCC 3 cut(s) 334, 426, 438
SchI GAGTC 1 cut(s) 372
ScrFI CCNGG 3 cut(s) 369, 378, 388
SduI GDGCHC 2 cut(s) 8, 264
SetI ASST 8 cut(s) 89, 108, 254, 264, 354, 382, 488, 530
SfaNI GCATC 1 cut(s) 200
SinI GGWCC 2 cut(s) 334, 426
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
Sse9I AATT 4 cut(s) 42, 109, 507, 541
SsiI CCGC 2 cut(s) 55, 293
SspMI CTAG 3 cut(s) 188, 255, 353
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 3 cut(s) 367, 376, 386
StyI CCWWGG 1 cut(s) 145
TaaI ACNGT 1 cut(s) 16
TaiI ACGT 1 cut(s) 530
TaqI TCGA 2 cut(s) 75, 493
TasI AATT 4 cut(s) 42, 109, 507, 541
TauI GCSGC 1 cut(s) 295
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 3 cut(s) 123, 233, 482
Tru9I TTAA 3 cut(s) 123, 233, 482
TscAI CASTG 3 cut(s) 19, 102, 157
TseFI GTSAC 3 cut(s) 50, 225, 382
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 3 cut(s) 50, 225, 382
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 3 cut(s) 19, 102, 157
Tth111I GACNNNGTC 1 cut(s) 362
Van91I CCANNNNNTGG 1 cut(s) 435
VpaK11BI GGWCC 2 cut(s) 334, 426
XapI RAATTY 1 cut(s) 42
XbaI TCTAGA 1 cut(s) 254
XspI CTAG 3 cut(s) 188, 255, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.