pycom15g09270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0028354
Physical Location & Seq
Reverse (-)
5988175 .. 5991884
3710 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g09270.9

Sequence Viewer

Length: 870 bp
ATGGCGCCCTCCAAGCTCGCGATTCTCTCAATCTTTCTTGCCCTAATTTTCTCCCAGATTAGGGTTGAGGCTGATATTCCTCGTGGTCCATACAAGGGAGTGTGTCTTTCGGAGGAAGCATTGGCAAAGCTCAGTATAATACCACATCGGGAGCCCTCCGCAGAATCCAACCGCGGCGAGAAGAATAAGGAACAAAATAATGAGCATAGCAATAAAGGAATTCAAAAGGAGTCGGATATAATTTCTTCCTTGCGGAGTGAAATCGAGTCTCTCCAGAAAAACGGGTCATTAGTTGAGCAGCAGGTTGGGAAGGCTCAAGCCCGTGCCGATGAACTAGAGAAAAAGGTTAAGGAACTTGAACAGAAATTACAATCACAACGTAAGGAGAAAGAGGACTTGGAATCCCGGGCAAATGAAGCTGAGGAGAAGATCAATAATTTGCTTTCAGAAATAGAGAATACTGTTAAGGCTAATGATGAACAGGAGACCAAAATGCATAAAACTGACCATGCTCTTGAAGTTAAAGTGGCTGAGGTGGAAATGTGGAAGGCAAAGTTTGAAGCTACTCTAAAGGAAAAAGAGTTAAATGAGGTTCATTCTATGCAAGTTAACAAACTTCAAAAGGAATCGCAACGTAATGAGAAGGGGGACTTGAAAGACCGGGTAAATGAAGCCGAGAAGAAGATCAATGATTTGTTTTCAAAAATAGAGAATGTTCAAGGTACAGTAAAAGACTTGCTGGAGAGGCATGAACTGACAAGACCTCTTGCTACAAAGGAGTTGGAGTGGTTTGCGGCCTCGGCTTTGTTGGCCCTGCTCATTATAATTCTGGTTCAGAACTTTCTCCTGACTTTTCAGTACAAACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

290

Amino Acids

33.07

Weight (kDa)

5.87

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028354)

Species Orthologous Gene IDs
malus_domestica MD15G1100400.v1.1
pyrus_communis pycom15g09270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 824
Acc36I ACCTGC 1 cut(s) 292
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 2 cut(s) 20, 174
AciI CCGC 5 cut(s) 159, 172, 174, 253, 794
AcsI RAATTY 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 724, 860
AfiI CCNNNNNNNGG 3 cut(s) 60, 61, 95
AgsI TTSAA 8 cut(s) 224, 359, 518, 560, 620, 655, 702, 719
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AluBI AGCT 4 cut(s) 16, 130, 419, 563
AluI AGCT 4 cut(s) 16, 130, 419, 563
Alw26I GTCTC 2 cut(s) 273, 479
Ama87I CYCGRG 1 cut(s) 405
AoxI GGCC 2 cut(s) 795, 810
ApeKI GCWGC 1 cut(s) 298
ApoI RAATTY 1 cut(s) 219
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 86, 811
AsuC2I CCSGG 3 cut(s) 406, 407, 662
AvaI CYCGRG 1 cut(s) 405
AvaII GGWCC 1 cut(s) 86
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 156
BauI CACGAG 1 cut(s) 81
BbvCI CCTCAGC 2 cut(s) 420, 531
BbvI GCAGC 1 cut(s) 310
BcnI CCSGG 3 cut(s) 406, 407, 662
BcoDI GTCTC 2 cut(s) 273, 479
BfaI CTAG 1 cut(s) 335
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 292
BisI GCNGC 3 cut(s) 175, 299, 795
BlsI GCNGC 3 cut(s) 176, 300, 796
Bme1390I CCNGG 3 cut(s) 406, 407, 662
Bme18I GGWCC 1 cut(s) 86
BmeT110I CYCGRG 1 cut(s) 405
BmgT120I GGNCC 2 cut(s) 86, 811
BmiI GGNNCC 2 cut(s) 6, 153
BmrFI CCNGG 3 cut(s) 406, 407, 662
BpmI CTGGAG 2 cut(s) 257, 761
Bpu10I CCTNAGC 2 cut(s) 420, 531
BpuEI CTTGAG 1 cut(s) 300
BpuMI CCSGG 3 cut(s) 406, 407, 662
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 479
BsaJI CCNNGG 3 cut(s) 172, 405, 798
BsaXI ACNNNNNCTCC 2 cut(s) 770, 800
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 61, 95
BseDI CCNNGG 3 cut(s) 172, 405, 798
BseLI CCNNNNNNNGG 3 cut(s) 60, 61, 95
BseMII CTCAG 3 cut(s) 145, 411, 522
BseRI GAGGAG 1 cut(s) 437
BseXI GCAGC 1 cut(s) 310
Bsh1236I CGCG 2 cut(s) 20, 174
BshFI GGCC 2 cut(s) 797, 812
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 1 cut(s) 405
BsiSI CCGG 2 cut(s) 406, 661
BslFI GGGAC 1 cut(s) 662
BslI CCNNNNNNNGG 3 cut(s) 60, 61, 95
BsmAI GTCTC 2 cut(s) 273, 479
BsmFI GGGAC 1 cut(s) 662
BsnI GGCC 2 cut(s) 797, 812
Bso31I GGTCTC 1 cut(s) 479
BsoBI CYCGRG 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 2 cut(s) 429, 684
Bsp68I TCGCGA 1 cut(s) 20
BspACI CCGC 5 cut(s) 159, 172, 174, 253, 794
BspANI GGCC 2 cut(s) 797, 812
BspCNI CTCAG 3 cut(s) 144, 412, 523
BspFNI CGCG 2 cut(s) 20, 174
BspLI GGNNCC 2 cut(s) 6, 153
BspMI ACCTGC 1 cut(s) 292
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 479
BssECI CCNNGG 3 cut(s) 172, 405, 798
BssMI GATC 2 cut(s) 429, 684
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 81
Bst2BI CACGAG 1 cut(s) 81
Bst4CI ACNGT 2 cut(s) 463, 727
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 3 cut(s) 131, 420, 531
BstDSI CCRYGG 1 cut(s) 172
BstFNI CGCG 2 cut(s) 20, 174
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 432, 687
BstMAI GTCTC 2 cut(s) 273, 479
BstMBI GATC 2 cut(s) 429, 684
BstMWI GCNNNNNNNGC 5 cut(s) 13, 416, 745, 800, 809
BstSCI CCNGG 3 cut(s) 404, 405, 660
BstUI CGCG 2 cut(s) 20, 174
BstV1I GCAGC 1 cut(s) 310
BsuRI GGCC 2 cut(s) 797, 812
BtgI CCRYGG 1 cut(s) 172
BtuMI TCGCGA 1 cut(s) 20
BveI ACCTGC 1 cut(s) 292
Cac8I GCNNGC 1 cut(s) 18
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 86, 811
Cfr42I CCGCGG 1 cut(s) 175
Cfr9I CCCGGG 1 cut(s) 405
Csp6I GTAC 2 cut(s) 723, 859
CviAII CATG 2 cut(s) 509, 749
CviQI GTAC 2 cut(s) 723, 859
DdeI CTNAG 3 cut(s) 131, 420, 531
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 431, 686
DpnII GATC 2 cut(s) 429, 684
Eco24I GRGCYC 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 405
EcoRI GAATTC 1 cut(s) 219
EcoT22I ATGCAT 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 156
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
FaeI CATG 2 cut(s) 512, 752
FaiI YATR 9 cut(s) 91, 137, 207, 239, 498, 510, 602, 750, 824
FalI AAGNNNNNCTT 2 cut(s) 635, 667
FaqI GGGAC 1 cut(s) 662
FatI CATG 2 cut(s) 508, 748
Fnu4HI GCNGC 3 cut(s) 175, 299, 795
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 3 cut(s) 175, 299, 795
FspBI CTAG 1 cut(s) 335
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 175, 299, 795
GsuI CTGGAG 2 cut(s) 257, 761
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 797, 812
HapII CCGG 2 cut(s) 406, 661
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 512, 752
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HinfI GANTC 6 cut(s) 22, 164, 230, 266, 401, 626
HpaI GTTAAC 1 cut(s) 610
HpaII CCGG 2 cut(s) 406, 661
Hpy166II GTNNAC 1 cut(s) 610
Hpy188I TCNGA 4 cut(s) 112, 235, 448, 837
Hpy188III TCNNGA 5 cut(s) 19, 149, 274, 515, 847
Hpy8I GTNNAC 1 cut(s) 610
HpyAV CCTTC 3 cut(s) 304, 541, 637
HpyCH4III ACNGT 2 cut(s) 463, 727
HpyCH4IV ACGT 2 cut(s) 379, 634
HpyCH4V TGCA 2 cut(s) 496, 604
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 416, 745, 800, 809
HpyF3I CTNAG 3 cut(s) 131, 420, 531
HpySE526I ACGT 2 cut(s) 379, 634
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 512, 752
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KspAI GTTAAC 1 cut(s) 610
KspI CCGCGG 1 cut(s) 175
Kzo9I GATC 2 cut(s) 429, 684
LmnI GCTCC 1 cut(s) 151
Lsp1109I GCAGC 1 cut(s) 310
MaeI CTAG 1 cut(s) 335
MaeII ACGT 2 cut(s) 379, 634
MalI GATC 2 cut(s) 431, 686
MboI GATC 2 cut(s) 429, 684
MboII GAAGA 5 cut(s) 193, 237, 439, 691, 694
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 6 cut(s) 45, 219, 240, 365, 436, 825
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 239, 275
MmeI TCCRAC 3 cut(s) 192, 213, 762
Mph1103I ATGCAT 1 cut(s) 498
MseI TTAA 5 cut(s) 348, 465, 522, 584, 609
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 2 cut(s) 406, 661
MspR9I CCNGG 3 cut(s) 406, 407, 662
MvnI CGCG 2 cut(s) 20, 174
MwoI GCNNNNNNNGC 5 cut(s) 13, 416, 745, 800, 809
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 3 cut(s) 406, 407, 662
NdeII GATC 2 cut(s) 429, 684
NlaIII CATG 2 cut(s) 512, 752
NlaIV GGNNCC 2 cut(s) 6, 153
NmeAIII GCCGAG 2 cut(s) 700, 779
NruI TCGCGA 1 cut(s) 20
NsiI ATGCAT 1 cut(s) 498
PfeI GAWTC 4 cut(s) 22, 164, 401, 626
PkrI GCNGC 3 cut(s) 176, 300, 796
PleI GAGTC 2 cut(s) 238, 274
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 238, 274
PsiI TTATAA 1 cut(s) 824
PspN4I GGNNCC 2 cut(s) 6, 153
PspPI GGNCC 2 cut(s) 86, 811
PsrI GAACNNNNNNTAC 2 cut(s) 351, 383
RruI TCGCGA 1 cut(s) 20
RsaI GTAC 2 cut(s) 724, 860
RsaNI GTAC 2 cut(s) 723, 859
SacII CCGCGG 1 cut(s) 175
SaqAI TTAA 5 cut(s) 348, 465, 522, 584, 609
SatI GCNGC 3 cut(s) 175, 299, 795
Sau3AI GATC 2 cut(s) 429, 684
Sau96I GGNCC 2 cut(s) 86, 811
SchI GAGTC 2 cut(s) 239, 275
ScrFI CCNGG 3 cut(s) 406, 407, 662
SduI GDGCHC 1 cut(s) 156
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 175
SgrBI CCGCGG 1 cut(s) 175
SinI GGWCC 1 cut(s) 86
SmaI CCCGGG 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
Sse9I AATT 6 cut(s) 45, 219, 240, 365, 436, 825
SsiI CCGC 5 cut(s) 159, 172, 174, 253, 794
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 335
StyD4I CCNGG 3 cut(s) 404, 405, 660
TaaI ACNGT 2 cut(s) 463, 727
TaiI ACGT 2 cut(s) 382, 637
TaqI TCGA 1 cut(s) 264
TasI AATT 6 cut(s) 45, 219, 240, 365, 436, 825
TatI WGTACW 1 cut(s) 858
TauI GCSGC 2 cut(s) 177, 797
TfiI GAWTC 4 cut(s) 22, 164, 401, 626
Tru1I TTAA 5 cut(s) 348, 465, 522, 584, 609
Tru9I TTAA 5 cut(s) 348, 465, 522, 584, 609
TseI GCWGC 1 cut(s) 298
TspDTI ATGAA 6 cut(s) 345, 429, 492, 584, 684, 765
TspMI CCCGGG 1 cut(s) 405
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 1 cut(s) 219
XmaI CCCGGG 1 cut(s) 405
XspI CTAG 1 cut(s) 335
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.