pycom15g12680

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0029368
Physical Location & Seq
Reverse (-)
8677448 .. 8679768
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g12680.1

Sequence Viewer

Length: 450 bp
ATGTTCTACGTTTTTCGGGAGTTGTTGATCCCTCAATCGTCTCCCCATGTTGTTTTAAAGCGCCCAAACCCAAACCCTACCCTCAGACCAAGGAAGGAAGCAAAGATAGAGATATGGCGACTCCGAGCTCAGCCAAGAAGTCGTACGAGAACGCCAAAGCAGAAGGGAGCCGTCGGTAACATGTTGAAGAAGAGAGGGCAGTACGCGGAGGCCCTCAAGTGGCCAACATCGACTACCATCTTTCTGTCAAGTACTTGTGCCAAAAGCATTTCCTCCCCGCCTGGCGCTTGTCAGTCTATCGGCGAGCTCTATCTTTGCCTCGAGGTTTTCCACACGCTCTTCTTTTTCAGCGAGATGGTGTCTAGATTGGTGACGTTGCAGCCCGTGTTGCAGGGAGATTTGATAGCAGGCTACTGCAATTCAAGTTGGGCTGTTTCTTGCCATCTGTGA

Protein Analysis

150

Amino Acids

16.85

Weight (kDa)

9.98

Isoelectric Point (pI)

60.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029368)

Species Orthologous Gene IDs
pyrus_communis pycom09g17150 pycom15g12680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 320
AccII CGCG 1 cut(s) 206
AciI CCGC 2 cut(s) 206, 278
AclWI GGATC 1 cut(s) 22
AcoI YGGCCR 1 cut(s) 221
AfaI GTAC 3 cut(s) 145, 203, 253
AfiI CCNNNNNNNGG 1 cut(s) 219
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 2 cut(s) 187, 423
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 2 cut(s) 128, 307
AluI AGCT 2 cut(s) 128, 307
Alw21I GWGCWC 2 cut(s) 130, 309
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 1 cut(s) 22
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 2 cut(s) 210, 221
ApeKI GCWGC 1 cut(s) 379
AspLEI GCGC 2 cut(s) 63, 287
AspS9I GGNCC 1 cut(s) 211
AsuHPI GGTGA 1 cut(s) 382
AvaI CYCGRG 1 cut(s) 320
BalI TGGCCA 1 cut(s) 223
BanII GRGCYC 2 cut(s) 130, 309
Bbv12I GWGCWC 2 cut(s) 130, 309
BbvI GCAGC 1 cut(s) 391
BccI CCATC 2 cut(s) 245, 349
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 1 cut(s) 363
BfoI RGCGCY 2 cut(s) 64, 288
BisI GCNGC 1 cut(s) 380
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 381
BmcAI AGTACT 1 cut(s) 253
Bme1390I CCNGG 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 89
Bsc4I CCNNNNNNNGG 1 cut(s) 219
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 89
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMII CTCAG 2 cut(s) 97, 143
BseXI GCAGC 1 cut(s) 391
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 2 cut(s) 212, 223
BsiHKAI GWGCWC 2 cut(s) 130, 309
BsiHKCI CYCGRG 1 cut(s) 320
BsiWI CGTACG 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 1 cut(s) 45
BsmBI CGTCTC 1 cut(s) 45
BsnI GGCC 2 cut(s) 212, 223
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 130, 309
Bsp143I GATC 1 cut(s) 27
Bsp1720I GCTNAGC 1 cut(s) 129
BspACI CCGC 2 cut(s) 206, 278
BspANI GGCC 2 cut(s) 212, 223
BspCNI CTCAG 2 cut(s) 96, 142
BspFNI CGCG 1 cut(s) 206
BspLI GGNNCC 1 cut(s) 169
BspPI GGATC 1 cut(s) 22
BspQI GCTCTTC 1 cut(s) 344
BssECI CCNNGG 1 cut(s) 89
BssMI GATC 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 282
Bst6I CTCTTC 2 cut(s) 185, 344
BstC8I GCNNGC 2 cut(s) 305, 409
BstDEI CTNAG 2 cut(s) 83, 129
BstFNI CGCG 1 cut(s) 206
BstH2I RGCGCY 2 cut(s) 64, 288
BstHHI GCGC 2 cut(s) 63, 287
BstKTI GATC 1 cut(s) 30
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 1 cut(s) 388
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 280
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 1 cut(s) 391
BsuRI GGCC 2 cut(s) 212, 223
Cac8I GCNNGC 2 cut(s) 305, 409
CfoI GCGC 2 cut(s) 63, 287
Cfr13I GGNCC 1 cut(s) 211
Csp6I GTAC 3 cut(s) 144, 202, 252
CviAII CATG 2 cut(s) 47, 181
CviJI RGCY 9 cut(s) 128, 133, 170, 212, 223, 307, 382, 411, 431
CviKI_1 RGCY 9 cut(s) 128, 133, 170, 212, 223, 307, 382, 411, 431
CviQI GTAC 3 cut(s) 144, 202, 252
DdeI CTNAG 2 cut(s) 83, 129
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
DraI TTTAAA 1 cut(s) 57
EaeI YGGCCR 1 cut(s) 221
Eam1104I CTCTTC 2 cut(s) 185, 344
EarI CTCTTC 2 cut(s) 185, 344
Ecl136II GAGCTC 2 cut(s) 128, 307
Eco130I CCWWGG 1 cut(s) 89
Eco24I GRGCYC 2 cut(s) 130, 309
Eco53kI GAGCTC 2 cut(s) 128, 307
Eco88I CYCGRG 1 cut(s) 320
EcoICRI GAGCTC 2 cut(s) 128, 307
EcoO109I RGGNCCY 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 2 cut(s) 130, 309
ErhI CCWWGG 1 cut(s) 89
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 2 cut(s) 50, 184
FaiI YATR 3 cut(s) 48, 115, 182
FatI CATG 2 cut(s) 46, 180
FauI CCCGC 1 cut(s) 285
Fnu4HI GCNGC 1 cut(s) 380
FriOI GRGCYC 2 cut(s) 130, 309
Fsp4HI GCNGC 1 cut(s) 380
FspBI CTAG 1 cut(s) 363
GlaI GCGC 2 cut(s) 62, 286
GluI GCNGC 1 cut(s) 380
HaeII RGCGCY 2 cut(s) 64, 288
HaeIII GGCC 2 cut(s) 212, 223
HhaI GCGC 2 cut(s) 63, 287
Hin1II CATG 2 cut(s) 50, 184
Hin6I GCGC 2 cut(s) 61, 285
HinP1I GCGC 2 cut(s) 61, 285
HinfI GANTC 1 cut(s) 120
HphI GGTGA 1 cut(s) 382
Hpy188I TCNGA 2 cut(s) 86, 125
Hpy188III TCNNGA 2 cut(s) 17, 363
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 2 cut(s) 88, 157
HpyCH4IV ACGT 2 cut(s) 9, 374
HpyCH4V TGCA 3 cut(s) 379, 391, 417
HpyF10VI GCNNNNNNNGC 1 cut(s) 388
HpyF3I CTNAG 2 cut(s) 83, 129
HpySE526I ACGT 2 cut(s) 9, 374
Hsp92II CATG 2 cut(s) 50, 184
HspAI GCGC 2 cut(s) 61, 285
Kzo9I GATC 1 cut(s) 27
LguI GCTCTTC 1 cut(s) 344
LmnI GCTCC 1 cut(s) 167
LpnPI CCDG 4 cut(s) 267, 294, 377, 393
Lsp1109I GCAGC 1 cut(s) 391
MaeI CTAG 1 cut(s) 363
MaeII ACGT 2 cut(s) 9, 374
MaeIII GTNAC 2 cut(s) 176, 370
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 3 cut(s) 199, 202, 331
MhlI GDGCHC 2 cut(s) 130, 309
MlsI TGGCCA 1 cut(s) 223
MluCI AATT 1 cut(s) 418
MluNI TGGCCA 1 cut(s) 223
MlyI GAGTC 1 cut(s) 114
MnlI CCTC 8 cut(s) 42, 92, 188, 202, 224, 283, 316, 329
Mox20I TGGCCA 1 cut(s) 223
MscI TGGCCA 1 cut(s) 223
MseI TTAA 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 223
MspR9I CCNGG 1 cut(s) 282
MvaI CCWGG 1 cut(s) 282
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 1 cut(s) 388
NdeII GATC 1 cut(s) 27
NlaIII CATG 2 cut(s) 50, 184
NlaIV GGNNCC 1 cut(s) 169
NmuCI GTSAC 1 cut(s) 370
NspI RCATGY 1 cut(s) 184
PaeR7I CTCGAG 1 cut(s) 320
PciI ACATGT 1 cut(s) 180
PciSI GCTCTTC 1 cut(s) 344
Pfl23II CGTACG 1 cut(s) 143
PkrI GCNGC 1 cut(s) 381
PleI GAGTC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 114
PscI ACATGT 1 cut(s) 180
Psp124BI GAGCTC 2 cut(s) 130, 309
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspLI CGTACG 1 cut(s) 143
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 1 cut(s) 211
PspXI VCTCGAGB 1 cut(s) 320
RsaI GTAC 3 cut(s) 145, 203, 253
RsaNI GTAC 3 cut(s) 144, 202, 252
SacI GAGCTC 2 cut(s) 130, 309
SapI GCTCTTC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 1 cut(s) 380
Sau3AI GATC 1 cut(s) 27
Sau96I GGNCC 1 cut(s) 211
ScaI AGTACT 1 cut(s) 253
SchI GAGTC 1 cut(s) 114
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 2 cut(s) 130, 309
SetI ASST 5 cut(s) 12, 130, 309, 327, 377
Sfr274I CTCGAG 1 cut(s) 320
SlaI CTCGAG 1 cut(s) 320
SmlI CTYRAG 2 cut(s) 215, 320
SmoI CTYRAG 2 cut(s) 215, 320
Sse9I AATT 1 cut(s) 418
SsiI CCGC 2 cut(s) 206, 278
SspMI CTAG 1 cut(s) 363
SstI GAGCTC 2 cut(s) 130, 309
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 1 cut(s) 89
TaiI ACGT 2 cut(s) 12, 377
TaqI TCGA 2 cut(s) 230, 321
TasI AATT 1 cut(s) 418
TatI WGTACW 1 cut(s) 251
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 1 cut(s) 379
Tsp45I GTSAC 1 cut(s) 370
XbaI TCTAGA 1 cut(s) 362
XceI RCATGY 1 cut(s) 184
XhoI CTCGAG 1 cut(s) 320
XspI CTAG 1 cut(s) 363
ZrmI AGTACT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.