pycom15g22830

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0024700
Physical Location & Seq
Forward (+)
16916372 .. 16917237
866 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 450 bp
ATGACAAGGGTTGGATTCCTCGAGCTCCAAGATGAGTCTGGTTTGACACTAGCATGCATAGACAAATGTAGAGATGGTGGATTTGAAGAAATTTTTATGACCACGGTGGACTATCCTGTAAAATATGACCACATCATAAGGTTGAACTTAAAGGGGAACAGTGTAGTTTATACATCAGGATTTTGCTTTGATGATGAATGTTGGAGATTGTATGAGCAGAAGGTGCAGAGTATTTTAATTCAAGGGTTGAGTGACAGAGTGAAGAATGTACGTGTTACCTGGAGAAATATGTTATCAGACCACATCATAAAAAATGGACTGTCAACCCTGAATGCAGAACCATTGCTTATTGGAATTACCGTGAGCTCTTTAGAGAAAGCTTTTAGGCTTGTTGATATTGGTCCAGATGCTGATAATAAAGAGGAGGCTCTCAAATTCCGCAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

150

Amino Acids

17.04

Weight (kDa)

5.42

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap_D3 PF17404 40 - 149 1.8e-14 Nrap protein domain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024700)

Species Orthologous Gene IDs
pyrus_communis pycom15g22830
rosa_chinensis RchiOBHm_Chr5g0064711
rosa_samantha Rh5AG424300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 439
AcsI RAATTY 2 cut(s) 90, 434
AfaI GTAC 1 cut(s) 270
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 3 cut(s) 86, 145, 242
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 3 cut(s) 25, 366, 380
AluI AGCT 3 cut(s) 25, 366, 380
Alw21I GWGCWC 2 cut(s) 27, 368
AlwNI CAGNNNCTG 1 cut(s) 410
Ama87I CYCGRG 1 cut(s) 20
ApoI RAATTY 2 cut(s) 90, 434
AspS9I GGNCC 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 1 cut(s) 401
BanII GRGCYC 2 cut(s) 27, 368
Bbv12I GWGCWC 2 cut(s) 27, 368
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 280
BfaI CTAG 2 cut(s) 50, 448
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 401
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 401
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 1 cut(s) 397
BpmI CTGGAG 1 cut(s) 301
BsaAI YACGTR 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 341
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 341
BseRI GAGGAG 1 cut(s) 437
BsgI GTGCAG 1 cut(s) 245
BsiHKAI GWGCWC 2 cut(s) 27, 368
BsiHKCI CYCGRG 1 cut(s) 20
BsmI GAATGC 1 cut(s) 337
BsoBI CYCGRG 1 cut(s) 20
Bsp1286I GDGCHC 2 cut(s) 27, 368
BspACI CCGC 1 cut(s) 439
BsrDI GCAATG 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 4 cut(s) 106, 161, 321, 361
BstAPI GCANNNNNTGC 1 cut(s) 223
BstBAI YACGTR 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 55
BstDSI CCRYGG 1 cut(s) 102
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstNI CCWGG 1 cut(s) 280
BstNSI RCATGY 1 cut(s) 57
BstSCI CCNGG 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 102
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 1 cut(s) 55
CaiI CAGNNNCTG 1 cut(s) 410
Cfr13I GGNCC 1 cut(s) 401
Csp6I GTAC 1 cut(s) 269
CviAII CATG 1 cut(s) 54
CviJI RGCY 5 cut(s) 25, 366, 380, 388, 428
CviKI_1 RGCY 5 cut(s) 25, 366, 380, 388, 428
CviQI GTAC 1 cut(s) 269
Ecl136II GAGCTC 2 cut(s) 25, 366
Eco24I GRGCYC 2 cut(s) 27, 368
Eco47I GGWCC 1 cut(s) 401
Eco53kI GAGCTC 2 cut(s) 25, 366
Eco88I CYCGRG 1 cut(s) 20
EcoICRI GAGCTC 2 cut(s) 25, 366
EcoRII CCWGG 1 cut(s) 278
EcoT22I ATGCAT 1 cut(s) 59
EcoT38I GRGCYC 2 cut(s) 27, 368
FaeI CATG 1 cut(s) 57
FaiI YATR 9 cut(s) 55, 59, 98, 126, 137, 171, 213, 290, 308
FatI CATG 1 cut(s) 53
FriOI GRGCYC 2 cut(s) 27, 368
FspBI CTAG 2 cut(s) 50, 448
GsuI CTGGAG 1 cut(s) 301
Hin1II CATG 1 cut(s) 57
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 2 cut(s) 15, 35
Hpy166II GTNNAC 2 cut(s) 109, 324
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 2 cut(s) 177, 404
Hpy8I GTNNAC 2 cut(s) 109, 324
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 4 cut(s) 106, 161, 321, 361
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 3 cut(s) 57, 226, 335
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpySE526I ACGT 1 cut(s) 271
Hsp92II CATG 1 cut(s) 57
LmnI GCTCC 1 cut(s) 30
LpnPI CCDG 7 cut(s) 24, 129, 162, 265, 292, 341, 417
LweI GCATC 1 cut(s) 397
MaeI CTAG 2 cut(s) 50, 448
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 2 cut(s) 251, 274
MboII GAAGA 2 cut(s) 98, 274
MhlI GDGCHC 2 cut(s) 27, 368
MluCI AATT 4 cut(s) 90, 237, 354, 434
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 182
MnlI CCTC 3 cut(s) 29, 415, 418
Mph1103I ATGCAT 1 cut(s) 59
MseI TTAA 2 cut(s) 149, 236
MslI CAYNNNNRTG 1 cut(s) 52
MspR9I CCNGG 1 cut(s) 280
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 1 cut(s) 280
MwoI GCNNNNNNNGC 1 cut(s) 223
NlaIII CATG 1 cut(s) 57
NmuCI GTSAC 1 cut(s) 251
NsiI ATGCAT 1 cut(s) 59
NspI RCATGY 1 cut(s) 57
PaeI GCATGC 1 cut(s) 57
PaeR7I CTCGAG 1 cut(s) 20
PctI GAATGC 1 cut(s) 337
PfeI GAWTC 1 cut(s) 15
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Ppu21I YACGTR 1 cut(s) 272
Psp124BI GAGCTC 2 cut(s) 27, 368
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspPI GGNCC 1 cut(s) 401
PspXI VCTCGAGB 1 cut(s) 20
PstNI CAGNNNCTG 1 cut(s) 410
RsaI GTAC 1 cut(s) 270
RsaNI GTAC 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 52
SacI GAGCTC 2 cut(s) 27, 368
SaqAI TTAA 2 cut(s) 149, 236
Sau96I GGNCC 1 cut(s) 401
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 2 cut(s) 27, 368
SetI ASST 7 cut(s) 27, 143, 225, 274, 281, 368, 382
SfaNI GCATC 1 cut(s) 397
Sfr274I CTCGAG 1 cut(s) 20
SinI GGWCC 1 cut(s) 401
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SphI GCATGC 1 cut(s) 57
Sse9I AATT 4 cut(s) 90, 237, 354, 434
SsiI CCGC 1 cut(s) 439
SspMI CTAG 2 cut(s) 50, 448
SstI GAGCTC 2 cut(s) 27, 368
StyD4I CCNGG 1 cut(s) 278
TaaI ACNGT 4 cut(s) 106, 161, 321, 361
TaiI ACGT 1 cut(s) 274
TaqI TCGA 1 cut(s) 21
TasI AATT 4 cut(s) 90, 237, 354, 434
TfiI GAWTC 1 cut(s) 15
Tru1I TTAA 2 cut(s) 149, 236
Tru9I TTAA 2 cut(s) 149, 236
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 1 cut(s) 210
TspRI CASTG 1 cut(s) 166
VpaK11BI GGWCC 1 cut(s) 401
XapI RAATTY 2 cut(s) 90, 434
XceI RCATGY 1 cut(s) 57
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
XhoI CTCGAG 1 cut(s) 20
XspI CTAG 2 cut(s) 50, 448
Zsp2I ATGCAT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.