pycom15g25200

Microtubule-associated protein RP EB family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0024355
Physical Location & Seq
Reverse (-)
19585923 .. 19587591
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 474 bp
ATGTGGACCCAAATTAGAATCCCGCTCCCCGAAACTCCTCCCAGTTTTAAATTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTACAGGGACGGAGAGACCTTACCAAAATCTCCAATGGCGACGAATATTGGTATGATGGACGGCGCTTACTTCGTAGGCAGGACCGAGATCTTGGCCTGGATCAACTCAACTCTCCATCTCAATCTCAGCAAAGTCGAAGAGGCGTGTTCCGGTGCCGTTCACTGCCAACTCATGGATGCCGTTCATAATGGTATCGTGCCGATGCACAAGGTCAATTTTGATGCCAAGAGCGAGTACGAGATGATTCAGAACTACAAGGTCCTTCAGGACGTTTTCAACAAACTCAAAATCACCAAGTTACAACGCTCCGGAGAGGAGAGAAGCTTGCAAAGGAGGGAAAGAAGGAAACAAGAGAGGTGCCCAATTGCAATCATCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000079 GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005819 GO:0005856 GO:0006355 GO:0006996 GO:0007010 GO:0008017 GO:0008022 GO:0008092 GO:0008104 GO:0008150 GO:0009524 GO:0009605 GO:0009606 GO:0009612 GO:0009628 GO:0009652 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0015630 GO:0015631 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0030312 GO:0030496 GO:0030865 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0033036 GO:0033674 GO:0042325 GO:0042327 GO:0042802 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043549 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0048471 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071840 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CH EB1

Protein Analysis

158

Amino Acids

17.95

Weight (kDa)

9.05

Isoelectric Point (pI)

52.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 58 - 132 1.1e-06 Calponin homology (CH) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024355)

Species Orthologous Gene IDs
malus_domestica MD16G1114300.v1.1
pyrus_communis pycom15g25200
rosa_multiflora Rmu_sc0004352.1_g000005

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 245, 450
AccB7I CCANNNNNTGG 1 cut(s) 265
AccBSI CCGCTC 1 cut(s) 25
AccIII TCCGGA 1 cut(s) 401
AciI CCGC 1 cut(s) 23
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 341
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 1 cut(s) 370
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 1 cut(s) 417
AluI AGCT 1 cut(s) 417
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 1 cut(s) 200
Aor13HI TCCGGA 1 cut(s) 401
AoxI GGCC 1 cut(s) 186
ApoI RAATTY 1 cut(s) 50
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 3 cut(s) 6, 174, 352
AsuHPI GGTGA 1 cut(s) 376
AvaII GGWCC 3 cut(s) 6, 174, 352
BaeGI GKGCMC 1 cut(s) 455
BanI GGYRCC 2 cut(s) 245, 450
BccI CCATC 2 cut(s) 142, 216
BceAI ACGGC 3 cut(s) 169, 233, 257
BciT130I CCWGG 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 101
BfmI CTRYAG 1 cut(s) 94
BfoI RGCGCY 1 cut(s) 159
BglII AGATCT 1 cut(s) 180
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 3 cut(s) 6, 174, 352
BmgT120I GGNCC 3 cut(s) 6, 174, 352
BmiI GGNNCC 3 cut(s) 8, 247, 452
BmrFI CCNGG 1 cut(s) 190
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 3 cut(s) 259, 285, 304
BmuI ACTGGG 1 cut(s) 36
BsaI GGTCTC 1 cut(s) 101
BsaWI WCCGGW 2 cut(s) 242, 401
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 42
BseAI TCCGGA 1 cut(s) 401
BseBI CCWGG 1 cut(s) 190
BseGI GGATG 1 cut(s) 274
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMII CTCAG 1 cut(s) 232
BseNI ACTGG 1 cut(s) 42
BseRI GAGGAG 2 cut(s) 27, 422
BseSI GKGCMC 1 cut(s) 455
BshFI GGCC 1 cut(s) 188
BshNI GGYRCC 2 cut(s) 245, 450
BsiSI CCGG 2 cut(s) 243, 402
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 113
BsnI GGCC 1 cut(s) 188
Bso31I GGTCTC 1 cut(s) 101
Bsp1286I GDGCHC 1 cut(s) 455
Bsp13I TCCGGA 1 cut(s) 401
Bsp143I GATC 2 cut(s) 180, 192
BspACI CCGC 1 cut(s) 23
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 1 cut(s) 231
BspEI TCCGGA 1 cut(s) 401
BspLI GGNNCC 3 cut(s) 8, 247, 452
BspPI GGATC 1 cut(s) 200
BspT107I GGYRCC 2 cut(s) 245, 450
BspTNI GGTCTC 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 25
BsrI ACTGG 1 cut(s) 42
BssMI GATC 2 cut(s) 180, 192
Bst2UI CCWGG 1 cut(s) 190
Bst6I CTCTTC 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 419
BstDEI CTNAG 1 cut(s) 218
BstF5I GGATG 1 cut(s) 274
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 2 cut(s) 183, 195
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 2 cut(s) 180, 192
BstNI CCWGG 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 188
BstSFI CTRYAG 1 cut(s) 94
BstSLI GKGCMC 1 cut(s) 455
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuRI GGCC 1 cut(s) 188
BtsCI GGATG 1 cut(s) 274
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 419
CfoI GCGC 1 cut(s) 158
Cfr13I GGNCC 3 cut(s) 6, 174, 352
Csp6I GTAC 1 cut(s) 328
CviAII CATG 1 cut(s) 265
CviJI RGCY 2 cut(s) 188, 417
CviKI_1 RGCY 2 cut(s) 188, 417
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 1 cut(s) 218
DpnI GATC 2 cut(s) 182, 194
DpnII GATC 2 cut(s) 180, 192
DraI TTTAAA 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 101
Eco47I GGWCC 3 cut(s) 6, 174, 352
Eco57I CTGAAG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 352
EcoRII CCWGG 1 cut(s) 188
FaeI CATG 1 cut(s) 268
FaiI YATR 3 cut(s) 146, 266, 279
FaqI GGGAC 1 cut(s) 113
FatI CATG 1 cut(s) 264
FauI CCCGC 1 cut(s) 30
FokI GGATG 1 cut(s) 281
GlaI GCGC 1 cut(s) 157
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 1 cut(s) 188
HapII CCGG 2 cut(s) 243, 402
HhaI GCGC 1 cut(s) 158
Hin1II CATG 1 cut(s) 268
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HindIII AAGCTT 1 cut(s) 415
HinfI GANTC 2 cut(s) 18, 337
HpaII CCGG 2 cut(s) 243, 402
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 2 cut(s) 6, 253
Hpy188I TCNGA 1 cut(s) 342
Hpy188III TCNNGA 2 cut(s) 359, 402
Hpy8I GTNNAC 2 cut(s) 6, 253
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 2 cut(s) 365, 429
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 3 cut(s) 298, 421, 461
HpyF3I CTNAG 1 cut(s) 218
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 1 cut(s) 268
HspAI GCGC 1 cut(s) 156
Kpn2I TCCGGA 1 cut(s) 401
Kzo9I GATC 2 cut(s) 180, 192
LmnI GCTCC 2 cut(s) 30, 404
LpnPI CCDG 8 cut(s) 55, 82, 157, 175, 202, 256, 344, 415
LweI GCATC 3 cut(s) 259, 285, 304
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 390
MalI GATC 2 cut(s) 182, 194
MbiI CCGCTC 1 cut(s) 25
MboI GATC 2 cut(s) 180, 192
MboII GAAGA 1 cut(s) 242
MfeI CAATTG 1 cut(s) 456
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 455
MluCI AATT 4 cut(s) 12, 50, 307, 456
MnlI CCTC 6 cut(s) 48, 65, 226, 400, 420, 441
MroI TCCGGA 1 cut(s) 401
MseI TTAA 1 cut(s) 48
MspI CCGG 2 cut(s) 243, 402
MspR9I CCNGG 1 cut(s) 190
MunI CAATTG 1 cut(s) 456
MvaI CCWGG 1 cut(s) 190
NdeII GATC 2 cut(s) 180, 192
NlaIII CATG 1 cut(s) 268
NlaIV GGNNCC 3 cut(s) 8, 247, 452
PfeI GAWTC 2 cut(s) 18, 337
PflMI CCANNNNNTGG 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 3 cut(s) 8, 247, 452
PspPI GGNCC 3 cut(s) 6, 174, 352
PspPPI RGGWCCY 1 cut(s) 352
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SaqAI TTAA 1 cut(s) 48
Sau3AI GATC 2 cut(s) 180, 192
Sau96I GGNCC 3 cut(s) 6, 174, 352
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 1 cut(s) 455
SetI ASST 6 cut(s) 113, 306, 354, 366, 419, 452
SfaNI GCATC 3 cut(s) 259, 285, 304
SfcI CTRYAG 1 cut(s) 94
SinI GGWCC 3 cut(s) 6, 174, 352
Sse9I AATT 4 cut(s) 12, 50, 307, 456
SsiI CCGC 1 cut(s) 23
SspI AATATT 1 cut(s) 139
StyD4I CCNGG 1 cut(s) 188
TaiI ACGT 1 cut(s) 366
TaqI TCGA 1 cut(s) 228
TaqII GACCGA 1 cut(s) 191
TasI AATT 4 cut(s) 12, 50, 307, 456
TfiI GAWTC 2 cut(s) 18, 337
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TscAI CASTG 1 cut(s) 260
TspDTI ATGAA 1 cut(s) 266
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 1 cut(s) 260
Van91I CCANNNNNTGG 1 cut(s) 265
VpaK11BI GGWCC 3 cut(s) 6, 174, 352
XapI RAATTY 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.