pycom15g33510

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0024701
Physical Location & Seq
Reverse (-)
32925235 .. 32925978
744 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g33510.1

Sequence Viewer

Length: 468 bp
ATGGATTGTAATGCTCGTTTAACCATTACTGGGGTTCACTTTCAGGAAGCATTGGCATCAATTGAGGAGCTTGAACCTTTTACCAATCATGCATCCCAAATGGCTGCGCTTGACTACATTGCTTCAGTTGAAAGTGATGTATTTATACCTACATACCCCGGACACATGGCGGCTACAGTTGAAGGTCACCGTCGCTTTCTTGGACACAGGAAAACAATATCTCCTGATAGAAAAGCCCTTGTTTCGATGTTTGACCAACTTGAGCAGGGAACTCTTGAGGAAGGCAAAGCCTTATCAAATAGAGTTGTTCAAATGCACAGGAAACGGCAAGGCTCCCCACGGAAGAGAAGAGGTCCCCTTCCAGGACTTAGGGGGAAGGATAAGTTTCGTTCAGAAGAACCATTTTACGAGAACCCCTTACCAGATTGTTTGTGTAGAAAAGAATTCCCAGTGCAAAGAACTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

156

Amino Acids

17.68

Weight (kDa)

9.12

Isoelectric Point (pI)

59.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 14 - 66 1.5e-12 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024701)

Species Orthologous Gene IDs
pyrus_communis pycom15g33510
rosa_multiflora Rmu_sc0034720.1_g000001 Rmu_sc0034721.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 170
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 30, 362
AgsI TTSAA 4 cut(s) 74, 131, 182, 311
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 1 cut(s) 70
AluI AGCT 1 cut(s) 70
ApeKI GCWGC 1 cut(s) 104
ApoI RAATTY 1 cut(s) 443
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 353
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 1 cut(s) 179
AvaII GGWCC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 91
BceAI ACGGC 1 cut(s) 341
BcgI CGANNNNNNTGC 1 cut(s) 443
BciT130I CCWGG 1 cut(s) 363
BcnI CCSGG 1 cut(s) 159
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 2 cut(s) 105, 171
BlsI GCNGC 2 cut(s) 106, 172
Bme1390I CCNGG 2 cut(s) 159, 363
Bme18I GGWCC 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 2 cut(s) 334, 355
BmrFI CCNGG 2 cut(s) 159, 363
BmrI ACTGGG 2 cut(s) 39, 443
BmsI GCATC 2 cut(s) 65, 101
BmuI ACTGGG 2 cut(s) 39, 443
BpuEI CTTGAG 2 cut(s) 281, 296
BpuMI CCSGG 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 157, 338
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 362
Bse1I ACTGG 2 cut(s) 34, 449
Bse3DI GCAATG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 2 cut(s) 157, 338
BseGI GGATG 1 cut(s) 92
BseLI CCNNNNNNNGG 2 cut(s) 30, 362
BseMI GCAATG 1 cut(s) 117
BseNI ACTGG 2 cut(s) 34, 449
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 91
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 339
BslI CCNNNNNNNGG 2 cut(s) 30, 362
BsmFI GGGAC 1 cut(s) 339
BspACI CCGC 1 cut(s) 170
BspLI GGNNCC 2 cut(s) 334, 355
BsrDI GCAATG 1 cut(s) 117
BsrI ACTGG 2 cut(s) 34, 449
BssECI CCNNGG 2 cut(s) 157, 338
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 178, 191
Bst6I CTCTTC 2 cut(s) 338, 343
BstDEI CTNAG 1 cut(s) 368
BstDSI CCRYGG 1 cut(s) 338
BstEII GGTNACC 1 cut(s) 185
BstF5I GGATG 1 cut(s) 92
BstHHI GCGC 1 cut(s) 109
BstNI CCWGG 1 cut(s) 363
BstPI GGTNACC 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 157, 361
BstSFI CTRYAG 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 91
BtgI CCRYGG 1 cut(s) 338
BtsCI GGATG 1 cut(s) 92
BtsIMutI CAGTG 1 cut(s) 456
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 1 cut(s) 353
CviAII CATG 2 cut(s) 89, 166
CviJI RGCY 6 cut(s) 70, 104, 173, 236, 290, 333
CviKI_1 RGCY 6 cut(s) 70, 104, 173, 236, 290, 333
DdeI CTNAG 1 cut(s) 368
Eam1104I CTCTTC 2 cut(s) 338, 343
EarI CTCTTC 2 cut(s) 338, 343
Eco47I GGWCC 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 108
Eco91I GGTNACC 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 353
EcoO65I GGTNACC 1 cut(s) 185
EcoRI GAATTC 1 cut(s) 443
EcoRII CCWGG 1 cut(s) 361
EcoT22I ATGCAT 1 cut(s) 94
FaeI CATG 2 cut(s) 92, 169
FaiI YATR 4 cut(s) 90, 146, 154, 167
FaqI GGGAC 1 cut(s) 339
FatI CATG 2 cut(s) 88, 165
Fnu4HI GCNGC 2 cut(s) 105, 171
FokI GGATG 1 cut(s) 79
Fsp4HI GCNGC 2 cut(s) 105, 171
GlaI GCGC 1 cut(s) 108
GluI GCNGC 2 cut(s) 105, 171
HapII CCGG 1 cut(s) 159
HhaI GCGC 1 cut(s) 109
Hin1II CATG 2 cut(s) 92, 169
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HpaII CCGG 1 cut(s) 159
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 394
Hpy188III TCNNGA 3 cut(s) 44, 224, 275
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 4 cut(s) 176, 275, 368, 370
HpyCH4III ACNGT 2 cut(s) 178, 191
HpyCH4V TGCA 3 cut(s) 92, 316, 454
HpyF3I CTNAG 1 cut(s) 368
Hsp92II CATG 2 cut(s) 92, 169
HspAI GCGC 1 cut(s) 107
LmnI GCTCC 2 cut(s) 67, 338
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 2 cut(s) 65, 101
MaeIII GTNAC 1 cut(s) 185
MboII GAAGA 3 cut(s) 355, 360, 407
MfeI CAATTG 1 cut(s) 60
MluCI AATT 2 cut(s) 60, 443
MnlI CCTC 3 cut(s) 58, 271, 344
Mph1103I ATGCAT 1 cut(s) 94
MseI TTAA 1 cut(s) 20
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 2 cut(s) 159, 363
MunI CAATTG 1 cut(s) 60
MvaI CCWGG 1 cut(s) 363
NciI CCSGG 1 cut(s) 159
NlaIII CATG 2 cut(s) 92, 169
NlaIV GGNNCC 2 cut(s) 334, 355
NmuCI GTSAC 1 cut(s) 185
NsiI ATGCAT 1 cut(s) 94
PfoI TCCNGGA 1 cut(s) 361
PkrI GCNGC 2 cut(s) 106, 172
PpuMI RGGWCCY 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 353
Psp6I CCWGG 1 cut(s) 361
PspEI GGTNACC 1 cut(s) 185
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 2 cut(s) 334, 355
PspPI GGNCC 1 cut(s) 353
PspPPI RGGWCCY 1 cut(s) 353
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 2 cut(s) 105, 171
Sau96I GGNCC 1 cut(s) 353
ScrFI CCNGG 2 cut(s) 159, 363
SetI ASST 5 cut(s) 72, 79, 151, 187, 355
SfaNI GCATC 2 cut(s) 65, 101
SfcI CTRYAG 1 cut(s) 174
SinI GGWCC 1 cut(s) 353
SmlI CTYRAG 2 cut(s) 260, 275
SmoI CTYRAG 2 cut(s) 260, 275
Sse9I AATT 2 cut(s) 60, 443
SsiI CCGC 1 cut(s) 170
StyD4I CCNGG 2 cut(s) 157, 361
TaaI ACNGT 2 cut(s) 178, 191
TaqI TCGA 1 cut(s) 245
TasI AATT 2 cut(s) 60, 443
TauI GCSGC 1 cut(s) 173
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 1 cut(s) 456
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 1 cut(s) 104
Tsp45I GTSAC 1 cut(s) 185
TspGWI ACGGA 1 cut(s) 355
TspRI CASTG 1 cut(s) 456
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 1 cut(s) 443
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.