pycom15g37550
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0022335
Physical Location & Seq
Reverse (-)
37476741 .. 37479940
3200 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1395 bp
ATGGCGATGATGATGGAGGATGGCCATGGCCAAGATTTGAGGAGCTTGGCGAGGACTTTAGAACGCGACCAAATTCTGGAAGTTTTGTCAGGCGGGTTTTGCTGCAGCTGCCAGCTCCTTTTGGAGAGTCGAATTCGCACCCTCCTCAACAAAAAAGTTGGTGAGGAGGTATCTTTTTCCAGTGATGATGATAATAGTTCTGCACAATTGAAGTCCATGGATGCACAAACCCCTTCACCATGCCAGTGTGTGGACGAACCAATTGTGCCGTCCTCAGAGAATGCTACGGAGTCATTCGATAAAATGCTTGACCAGCAAGAGTGCACTCCAATCCAAACACCTTATCATACAACTCCTGTCCACTCATCAAACCAAATAGAGTGCACGTCTAATTCATCTTCTCCGATGTTATTCGCTGGTGCAAAAAGTGGAGATTCCTCGGAGGATCCAAACGAGCATAACCGAGTATTACAAGTTCGGAGACAGAACTTCGTCCACATTGAGTATATACATGGCAGACGAATAAATGTCTTACAGGGCCTAGAGCTACATCCTCAGGTTTTTGATGCCGAGGAGCAGAAGCAAATTATCGAGTGTGTCTACAATTTACAACGTATGGGGAAAAGTGGACAACTTAAAGAGCGAACATATAGCGAACCTAGAAAGTGGATGCGTGGTAAAGGACGTGTGACAATACAATTTGGTTGTTGTTACAATTATGCAGTGGATAAGAAAGGGAACGCGCCAGGTATTGTTAGAGATGAAGATGTTGATCCTTTACCTCCTTTGTTCAAGCAAATGATAAACAGGATGGTTCGTTGGCATATATTACCTTCATCATGCATCCCGGACAGCTGCATTGTGAATATTTATGACGAAGGGGACTGCATTCCTCCTCATATTGACCACCATGATTTTGTTAGACCGTTTTGCACGGTTTCACTTTTGGCCGAATGCAACATACTTTTCGGTTCAAATCTAAAGGTTGTAGGACCAGGAGAATTTTCAGGACCTTATTCTATACGTTTGCCTGTCGGATCGGTGCTTCTTCTAGATGGTAATGCAGCTAACGTTGCTAAGCATTGCATACCTGGTGTTCCAGCAAGAAGGATTTCGATTACTTTCAGAAAAATGGATAGTAGCAAGCTACCGCACAATTTTTTCCATGATCCCGAGCTTCAGGGAAGTAAACCGTACGCTGTTTCTCCATTTGTCAAATCAAAAACCCAGCAAGATCAACGAGAGAAGCATTATTCCTATCTTCCTAGACGCAACCGTGGTGAATTCGAGAACGCTAAGAAGCGGAACCGCAACTCTTTTCTTCTTGAGAAAGATGAGTTTTCTCCACCTGGTAGATTCAGTTCTGGGAAGAGACGGATATTCAACAGACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

465

Amino Acids

52.59

Weight (kDa)

7.86

Isoelectric Point (pI)

67.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 180 - 376 8.6e-13 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022335)

Species Orthologous Gene IDs
malus_domestica MD15G1423500.v1.1
prunus_persica Prupe.1G569500_v2.0.a1 Prupe.1G569500_v2.0.a1
pyrus_communis pycom15g37550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 76, 250
AccI GTMKAC 1 cut(s) 600
AccII CGCG 2 cut(s) 66, 743
AciI CCGC 4 cut(s) 93, 1151, 1303, 1309
AclI AACGTT 1 cut(s) 1071
AclWI GGATC 5 cut(s) 440, 453, 767, 1045, 1163
AcoI YGGCCR 3 cut(s) 22, 28, 948
AcsI RAATTY 4 cut(s) 72, 132, 1001, 1283
AcuI CTGAAG 1 cut(s) 1163
AfaI GTAC 1 cut(s) 1196
AfiI CCNNNNNNNGG 2 cut(s) 76, 250
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 4 cut(s) 211, 793, 975, 1384
AjiI CACGTC 2 cut(s) 387, 686
AjnI CCWGG 4 cut(s) 745, 994, 1090, 1348
AluBI AGCT 8 cut(s) 45, 108, 115, 547, 855, 1067, 1147, 1177
AluI AGCT 8 cut(s) 45, 108, 115, 547, 855, 1067, 1147, 1177
Alw21I GWGCWC 2 cut(s) 326, 386
Alw26I GTCTC 2 cut(s) 475, 1366
Alw44I GTGCAC 2 cut(s) 322, 382
AlwI GGATC 5 cut(s) 440, 453, 767, 1045, 1163
Ama87I CYCGRG 1 cut(s) 1172
AoxI GGCC 4 cut(s) 22, 28, 538, 948
ApaLI GTGCAC 2 cut(s) 322, 382
ApeKI GCWGC 5 cut(s) 102, 105, 108, 855, 1064
ApoI RAATTY 4 cut(s) 72, 132, 1001, 1283
Asp700I GAANNNNTTC 1 cut(s) 1111
AspLEI GCGC 1 cut(s) 745
AspS9I GGNCC 3 cut(s) 538, 992, 1010
AsuC2I CCSGG 1 cut(s) 848
AsuHPI GGTGA 3 cut(s) 173, 228, 1292
AvaI CYCGRG 1 cut(s) 1172
AvaII GGWCC 2 cut(s) 992, 1010
AxyI CCTNAGG 1 cut(s) 555
BaeGI GKGCMC 2 cut(s) 326, 386
BalI TGGCCA 2 cut(s) 24, 30
BamHI GGATCC 1 cut(s) 445
Bbv12I GWGCWC 2 cut(s) 326, 386
BbvI GCAGC 5 cut(s) 89, 95, 117, 842, 1076
BccI CCATC 4 cut(s) 7, 14, 805, 1049
BceAI ACGGC 1 cut(s) 253
BciT130I CCWGG 4 cut(s) 747, 996, 1092, 1350
BcnI CCSGG 1 cut(s) 848
BcoDI GTCTC 2 cut(s) 475, 1366
BfaI CTAG 4 cut(s) 542, 660, 1052, 1266
BfmI CTRYAG 1 cut(s) 103
BisI GCNGC 5 cut(s) 103, 106, 109, 856, 1065
BlpI GCTNAGC 1 cut(s) 1077
BlsI GCNGC 5 cut(s) 104, 107, 110, 857, 1066
Bme1390I CCNGG 5 cut(s) 747, 848, 996, 1092, 1350
Bme18I GGWCC 2 cut(s) 992, 1010
BmeT110I CYCGRG 1 cut(s) 1172
BmgBI CACGTC 2 cut(s) 387, 686
BmgT120I GGNCC 3 cut(s) 538, 992, 1010
BmiI GGNNCC 2 cut(s) 447, 1307
BmrFI CCNGG 5 cut(s) 747, 848, 996, 1092, 1350
BmsI GCATC 4 cut(s) 211, 556, 660, 852
Bpu1102I GCTNAGC 1 cut(s) 1077
BpuEI CTTGAG 1 cut(s) 1346
BpuMI CCSGG 1 cut(s) 848
BsaBI GATNNNNATC 1 cut(s) 771
BsaJI CCNNGG 5 cut(s) 25, 216, 438, 570, 1276
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 250
Bse1I ACTGG 2 cut(s) 180, 244
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 1 cut(s) 1081
Bse8I GATNNNNATC 1 cut(s) 771
BseBI CCWGG 4 cut(s) 747, 996, 1092, 1350
BseDI CCNNGG 5 cut(s) 25, 216, 438, 570, 1276
BseGI GGATG 6 cut(s) 25, 226, 550, 675, 816, 843
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 2 cut(s) 76, 250
BseMI GCAATG 1 cut(s) 1081
BseMII CTCAG 2 cut(s) 288, 569
BseNI ACTGG 2 cut(s) 180, 244
BseRI GAGGAG 5 cut(s) 55, 134, 179, 587, 885
BseSI GKGCMC 2 cut(s) 326, 386
BseXI GCAGC 5 cut(s) 89, 95, 117, 842, 1076
BseYI CCCAGC 1 cut(s) 1227
BsgI GTGCAG 1 cut(s) 186
Bsh1236I CGCG 2 cut(s) 66, 743
BshFI GGCC 4 cut(s) 24, 30, 540, 950
BsiHKAI GWGCWC 2 cut(s) 326, 386
BsiHKCI CYCGRG 1 cut(s) 1172
BsiSI CCGG 1 cut(s) 848
BsiWI CGTACG 1 cut(s) 1194
BslFI GGGAC 1 cut(s) 896
BslI CCNNNNNNNGG 2 cut(s) 76, 250
BsmAI GTCTC 2 cut(s) 475, 1366
BsmBI CGTCTC 1 cut(s) 1366
BsmFI GGGAC 1 cut(s) 896
BsmI GAATGC 3 cut(s) 286, 888, 959
BsnI GGCC 4 cut(s) 24, 30, 540, 950
BsoBI CYCGRG 1 cut(s) 1172
Bsp1286I GDGCHC 2 cut(s) 326, 386
Bsp143I GATC 5 cut(s) 445, 772, 1037, 1168, 1234
Bsp1720I GCTNAGC 1 cut(s) 1077
Bsp19I CCATGG 2 cut(s) 25, 216
BspACI CCGC 4 cut(s) 93, 1151, 1303, 1309
BspANI GGCC 4 cut(s) 24, 30, 540, 950
BspCNI CTCAG 2 cut(s) 287, 568
BspFNI CGCG 2 cut(s) 66, 743
BspLI GGNNCC 2 cut(s) 447, 1307
BspMAI CTGCAG 1 cut(s) 107
BspPI GGATC 5 cut(s) 440, 453, 767, 1045, 1163
BsrDI GCAATG 1 cut(s) 1081
BsrI ACTGG 2 cut(s) 180, 244
BssECI CCNNGG 5 cut(s) 25, 216, 438, 570, 1276
BssMI GATC 5 cut(s) 445, 772, 1037, 1168, 1234
BssT1I CCWWGG 2 cut(s) 25, 216
Bst2UI CCWGG 4 cut(s) 747, 996, 1092, 1350
Bst4CI ACNGT 5 cut(s) 927, 937, 1194, 1277, 1392
Bst6I CTCTTC 1 cut(s) 1364
BstC8I GCNNGC 2 cut(s) 113, 1145
BstDEI CTNAG 4 cut(s) 274, 555, 1077, 1296
BstDSI CCRYGG 3 cut(s) 25, 216, 1276
BstF5I GGATG 6 cut(s) 25, 226, 550, 675, 816, 843
BstFNI CGCG 2 cut(s) 66, 743
BstHHI GCGC 1 cut(s) 745
BstKTI GATC 5 cut(s) 448, 775, 1040, 1171, 1237
BstMAI GTCTC 2 cut(s) 475, 1366
BstMBI GATC 5 cut(s) 445, 772, 1037, 1168, 1234
BstMWI GCNNNNNNNGC 4 cut(s) 99, 108, 313, 1073
BstNI CCWGG 4 cut(s) 747, 996, 1092, 1350
BstSCI CCNGG 5 cut(s) 745, 846, 994, 1090, 1348
BstSFI CTRYAG 1 cut(s) 103
BstSLI GKGCMC 2 cut(s) 326, 386
BstUI CGCG 2 cut(s) 66, 743
BstV1I GCAGC 5 cut(s) 89, 95, 117, 842, 1076
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
Bsu36I CCTNAGG 1 cut(s) 555
BsuRI GGCC 4 cut(s) 24, 30, 540, 950
BtgI CCRYGG 3 cut(s) 25, 216, 1276
BtgZI GCGATG 1 cut(s) 20
BtrI CACGTC 2 cut(s) 387, 686
BtsCI GGATG 6 cut(s) 25, 226, 550, 675, 816, 843
BtsI GCAGTG 1 cut(s) 729
BtsIMutI CAGTG 3 cut(s) 187, 251, 729
Cac8I GCNNGC 2 cut(s) 113, 1145
CfoI GCGC 1 cut(s) 745
Cfr13I GGNCC 3 cut(s) 538, 992, 1010
CseI GACGC 1 cut(s) 1278
CsiI ACCWGGT 2 cut(s) 1090, 1348
Csp6I GTAC 1 cut(s) 1195
CviAII CATG 7 cut(s) 26, 217, 240, 512, 840, 911, 1166
CviQI GTAC 1 cut(s) 1195
DdeI CTNAG 4 cut(s) 274, 555, 1077, 1296
DpnI GATC 5 cut(s) 447, 774, 1039, 1170, 1236
DpnII GATC 5 cut(s) 445, 772, 1037, 1168, 1234
EaeI YGGCCR 3 cut(s) 22, 28, 948
Eam1104I CTCTTC 1 cut(s) 1364
EarI CTCTTC 1 cut(s) 1364
Eco130I CCWWGG 2 cut(s) 25, 216
Eco47I GGWCC 2 cut(s) 992, 1010
Eco57I CTGAAG 1 cut(s) 1163
Eco81I CCTNAGG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 1172
EcoO109I RGGNCCY 2 cut(s) 538, 1010
EcoRI GAATTC 2 cut(s) 132, 1283
EcoRII CCWGG 4 cut(s) 745, 994, 1090, 1348
EcoT14I CCWWGG 2 cut(s) 25, 216
EcoT22I ATGCAT 1 cut(s) 845
ErhI CCWWGG 2 cut(s) 25, 216
Esp3I CGTCTC 1 cut(s) 1366
FaeI CATG 7 cut(s) 29, 220, 243, 515, 843, 914, 1169
FaqI GGGAC 1 cut(s) 896
FatI CATG 7 cut(s) 25, 216, 239, 511, 839, 910, 1165
FauI CCCGC 1 cut(s) 86
FblI GTMKAC 1 cut(s) 600
Fnu4HI GCNGC 5 cut(s) 103, 106, 109, 856, 1065
FokI GGATG 6 cut(s) 32, 233, 537, 682, 823, 830
Fsp4HI GCNGC 5 cut(s) 103, 106, 109, 856, 1065
FspBI CTAG 4 cut(s) 542, 660, 1052, 1266
GlaI GCGC 1 cut(s) 744
GluI GCNGC 5 cut(s) 103, 106, 109, 856, 1065
GsaI CCCAGC 1 cut(s) 1231
HaeIII GGCC 4 cut(s) 24, 30, 540, 950
HapII CCGG 1 cut(s) 848
HgaI GACGC 1 cut(s) 1278
HhaI GCGC 1 cut(s) 745
Hin1II CATG 7 cut(s) 29, 220, 243, 515, 843, 914, 1169
Hin6I GCGC 1 cut(s) 743
HinP1I GCGC 1 cut(s) 743
HinfI GANTC 4 cut(s) 127, 290, 434, 1356
HpaII CCGG 1 cut(s) 848
HphI GGTGA 3 cut(s) 173, 228, 1292
Hpy166II GTNNAC 8 cut(s) 253, 324, 361, 384, 496, 601, 629, 1190
Hpy188I TCNGA 6 cut(s) 277, 405, 442, 480, 1037, 1127
Hpy188III TCNNGA 6 cut(s) 77, 1008, 1052, 1172, 1288, 1325
Hpy8I GTNNAC 8 cut(s) 253, 324, 361, 384, 496, 601, 629, 1190
HpyAV CCTTC 4 cut(s) 243, 843, 872, 1101
HpyCH4III ACNGT 5 cut(s) 927, 937, 1194, 1277, 1392
HpyCH4IV ACGT 5 cut(s) 386, 613, 685, 1024, 1071
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 108, 313, 1073
HpyF3I CTNAG 4 cut(s) 274, 555, 1077, 1296
HpySE526I ACGT 5 cut(s) 386, 613, 685, 1024, 1071
Hsp92II CATG 7 cut(s) 29, 220, 243, 515, 843, 914, 1169
HspAI GCGC 1 cut(s) 743
Kzo9I GATC 5 cut(s) 445, 772, 1037, 1168, 1234
LmnI GCTCC 3 cut(s) 42, 120, 574
Lsp1109I GCAGC 5 cut(s) 89, 95, 117, 842, 1076
LweI GCATC 4 cut(s) 211, 556, 660, 852
MabI ACCWGGT 2 cut(s) 1090, 1348
MaeI CTAG 4 cut(s) 542, 660, 1052, 1266
MaeII ACGT 5 cut(s) 386, 613, 685, 1024, 1071
MaeIII GTNAC 2 cut(s) 688, 710
MalI GATC 5 cut(s) 447, 774, 1039, 1170, 1236
MboI GATC 5 cut(s) 445, 772, 1037, 1168, 1234
MboII GAAGA 6 cut(s) 390, 776, 1040, 1253, 1313, 1381
MfeI CAATTG 2 cut(s) 206, 261
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 2 cut(s) 326, 386
MlsI TGGCCA 2 cut(s) 24, 30
MluNI TGGCCA 2 cut(s) 24, 30
MlyI GAGTC 2 cut(s) 136, 299
MmeI TCCRAC 1 cut(s) 1015
Mox20I TGGCCA 2 cut(s) 24, 30
Mph1103I ATGCAT 1 cut(s) 845
MroXI GAANNNNTTC 1 cut(s) 1111
MscI TGGCCA 2 cut(s) 24, 30
MseI TTAA 1 cut(s) 636
MslI CAYNNNNRTG 1 cut(s) 244
Msp20I TGGCCA 2 cut(s) 24, 30
MspA1I CMGCKG 2 cut(s) 108, 855
MspI CCGG 1 cut(s) 848
MspR9I CCNGG 5 cut(s) 747, 848, 996, 1092, 1350
MunI CAATTG 2 cut(s) 206, 261
Mva1269I GAATGC 3 cut(s) 286, 888, 959
MvaI CCWGG 4 cut(s) 747, 996, 1092, 1350
MvnI CGCG 2 cut(s) 66, 743
MwoI GCNNNNNNNGC 4 cut(s) 99, 108, 313, 1073
NciI CCSGG 1 cut(s) 848
NcoI CCATGG 2 cut(s) 25, 216
NdeII GATC 5 cut(s) 445, 772, 1037, 1168, 1234
NlaIII CATG 7 cut(s) 29, 220, 243, 515, 843, 914, 1169
NlaIV GGNNCC 2 cut(s) 447, 1307
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 1 cut(s) 688
NsiI ATGCAT 1 cut(s) 845
PctI GAATGC 3 cut(s) 286, 888, 959
PdmI GAANNNNTTC 1 cut(s) 1111
PfeI GAWTC 2 cut(s) 434, 1356
Pfl23II CGTACG 1 cut(s) 1194
PflMI CCANNNNNTGG 2 cut(s) 76, 250
PfoI TCCNGGA 1 cut(s) 846
PkrI GCNGC 5 cut(s) 104, 107, 110, 857, 1066
PleI GAGTC 2 cut(s) 135, 298
PpsI GAGTC 2 cut(s) 135, 298
PpuMI RGGWCCY 1 cut(s) 1010
Psp1406I AACGTT 1 cut(s) 1071
Psp5II RGGWCCY 1 cut(s) 1010
Psp6I CCWGG 4 cut(s) 745, 994, 1090, 1348
PspFI CCCAGC 1 cut(s) 1227
PspGI CCWGG 4 cut(s) 745, 994, 1090, 1348
PspLI CGTACG 1 cut(s) 1194
PspN4I GGNNCC 2 cut(s) 447, 1307
PspPI GGNCC 3 cut(s) 538, 992, 1010
PspPPI RGGWCCY 1 cut(s) 1010
PsrI GAACNNNNNNTAC 4 cut(s) 459, 491, 1345, 1377
PstI CTGCAG 1 cut(s) 107
PsuI RGATCY 1 cut(s) 445
PvuII CAGCTG 2 cut(s) 108, 855
RsaI GTAC 1 cut(s) 1196
RsaNI GTAC 1 cut(s) 1195
RseI CAYNNNNRTG 1 cut(s) 244
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 5 cut(s) 103, 106, 109, 856, 1065
Sau3AI GATC 5 cut(s) 445, 772, 1037, 1168, 1234
Sau96I GGNCC 3 cut(s) 538, 992, 1010
SchI GAGTC 2 cut(s) 136, 299
ScrFI CCNGG 5 cut(s) 747, 848, 996, 1092, 1350
SduI GDGCHC 2 cut(s) 326, 386
SexAI ACCWGGT 2 cut(s) 1090, 1348
SfaNI GCATC 4 cut(s) 211, 556, 660, 852
SfcI CTRYAG 1 cut(s) 103
SinI GGWCC 2 cut(s) 992, 1010
SmiMI CAYNNNNRTG 1 cut(s) 244
SmlI CTYRAG 1 cut(s) 1325
SmoI CTYRAG 1 cut(s) 1325
SsiI CCGC 4 cut(s) 93, 1151, 1303, 1309
SspI AATATT 1 cut(s) 868
SspMI CTAG 4 cut(s) 542, 660, 1052, 1266
StyD4I CCNGG 5 cut(s) 745, 846, 994, 1090, 1348
StyI CCWWGG 2 cut(s) 25, 216
TaaI ACNGT 5 cut(s) 927, 937, 1194, 1277, 1392
TaiI ACGT 5 cut(s) 389, 616, 688, 1027, 1074
TaqI TCGA 5 cut(s) 130, 297, 591, 1115, 1287
TfiI GAWTC 2 cut(s) 434, 1356
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 3 cut(s) 187, 251, 729
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 5 cut(s) 102, 105, 108, 855, 1064
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 3 cut(s) 384, 777, 825
TspGWI ACGGA 2 cut(s) 302, 1390
TspRI CASTG 3 cut(s) 187, 251, 729
Van91I CCANNNNNTGG 2 cut(s) 76, 250
VneI GTGCAC 2 cut(s) 322, 382
VpaK11BI GGWCC 2 cut(s) 992, 1010
XapI RAATTY 4 cut(s) 72, 132, 1001, 1283
XbaI TCTAGA 1 cut(s) 1051
XmiI GTMKAC 1 cut(s) 600
XmnI GAANNNNTTC 1 cut(s) 1111
XspI CTAG 4 cut(s) 542, 660, 1052, 1266
Zsp2I ATGCAT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.