pycom16g10330

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0022566
Physical Location & Seq
Forward (+)
7053160 .. 7054996
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
ATGAGTTTATTCCATGTATTTGAGTTCGGTTTAAAGGTGGGGCATCTGCTTTGGATGCTATGTTGCTGGATTGTATCTGTAATTTCCATGAACTGGTACCTCACTGGTGGGATTGTGACAGACACCAAGGTAGGTTTGCTTGGTGAAGCTGCTAATATGTGCCTCAAATGGTGGGAGAAAATCCCAATGAACATCAGCAAGATCCGCCACCATTACTACAAGTATATCGGGTCGAAGAGAGTCCGAAAAACGTGGTGGAGGAGGCTTTTGTTTTCGTGGGTGGTTGGGTGGACAATTGGGAGCCTCTGGATCTTGTGGTACATGAGCTCACAGGCCAGTGAGAAGAGGAAGGAGACTCTTGCAAGTATGTGTGATGAGAGGGCGAGGATGTTGCAGGATCAGTTTAATGTCAGCATGAACCATATCCAGGCCATGTCGATACTGATCTCGACATTCCACCACGGAAAGTATCCGTCGGCCATTGATCAGAAAACTTTTGCGCGGTACACCGAAAGAACTGCTTTCGAGAGACCCCTCACAAGTGGCGTGGCATATGCTGTAAGGGTGCTCCACTCGGAAAAAGAACAATTTGAGAAGCAACAGGGCTGGACTATCAAGAGAATGGATACCCTCGAACAGAACCAGGTTCATAAGAATGACTATGCCCCAGAAGCCTTGGAACCTTCCCCAATTCAGGAAGAATATGCTCCTGTTATCTTTGCACAGGATACCGTTAGACATGTCATCTCTTTTGATATGCTGACCGGGAAGGAAGATCGACAAAACGTGTTGCGTGCAAGAGAATCGGGAAAGGGGGTTCTTACTGCTCCTTTTCGGTTGCTTAAAACAAACAGACTTGGAGTAATACTGACATTTGCCGTCTACAAAAGATCGATCTGCCCTCAAATGCAACTCCAAACGAAAGAATTCAAGCGACTGATGGGTATCTCGGTGGAATCTTTCATATTGAGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009743 GO:0009744 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0009991 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010150 GO:0010271 GO:0010959 GO:0012505 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034285 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042175 GO:0042221 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048366 GO:0048367 GO:0048509 GO:0048519 GO:0048580 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071396 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0090693 GO:0097305 GO:0097306 GO:0098542 GO:0098827 GO:0099402 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

326

Amino Acids

38.0

Weight (kDa)

9.57

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 160 - 318 1.2e-27 CHASE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022566)

Species Orthologous Gene IDs
pyrus_communis pycom16g05980 pycom16g10330
rosa_multiflora Rmu_co8003014.1_g000001 Rmu_sc0011796.1_g000009

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 93
AccI GTMKAC 1 cut(s) 882
AccII CGCG 1 cut(s) 502
AciI CCGC 2 cut(s) 205, 502
AclWI GGATC 3 cut(s) 196, 317, 405
AcoI YGGCCR 1 cut(s) 477
AcsI RAATTY 1 cut(s) 926
AfaI GTAC 3 cut(s) 98, 320, 506
AfiI CCNNNNNNNGG 3 cut(s) 93, 427, 694
AflIII ACRYGT 2 cut(s) 739, 786
AgsI TTSAA 1 cut(s) 931
AjnI CCWGG 2 cut(s) 426, 642
AloI GAACNNNNNNTCC 2 cut(s) 801, 833
AluBI AGCT 2 cut(s) 149, 327
AluI AGCT 2 cut(s) 149, 327
Alw21I GWGCWC 2 cut(s) 329, 570
Alw26I GTCTC 2 cut(s) 347, 523
AlwI GGATC 3 cut(s) 196, 317, 405
AoxI GGCC 3 cut(s) 333, 429, 477
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 1 cut(s) 926
Asp700I GAANNNNTTC 1 cut(s) 926
Asp718I GGTACC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 502
AsuC2I CCSGG 1 cut(s) 766
AsuHPI GGTGA 1 cut(s) 155
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 329
Bbv12I GWGCWC 2 cut(s) 329, 570
BbvI GCAGC 1 cut(s) 136
BccI CCATC 1 cut(s) 934
BceAI ACGGC 1 cut(s) 863
BcgI CGANNNNNNTGC 6 cut(s) 373, 407, 500, 534, 786, 820
BciT130I CCWGG 2 cut(s) 428, 644
BciVI GTATCC 3 cut(s) 480, 619, 721
BclI TGATCA 1 cut(s) 484
BcnI CCSGG 1 cut(s) 766
BcoDI GTCTC 2 cut(s) 347, 523
BfaI CTAG 1 cut(s) 976
BfuI GTATCC 3 cut(s) 480, 619, 721
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 3 cut(s) 428, 644, 766
BmiI GGNNCC 3 cut(s) 98, 302, 681
BmrFI CCNGG 3 cut(s) 428, 644, 766
BmsI GCATC 2 cut(s) 45, 52
BpuMI CCSGG 1 cut(s) 766
Bsa29I ATCGAT 1 cut(s) 893
BsaBI GATNNNNATC 2 cut(s) 443, 944
BsaI GGTCTC 1 cut(s) 523
BsaJI CCNNGG 3 cut(s) 126, 460, 675
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 427, 694
Bse1I ACTGG 3 cut(s) 98, 109, 336
Bse8I GATNNNNATC 2 cut(s) 443, 944
BseBI CCWGG 2 cut(s) 428, 644
BseCI ATCGAT 1 cut(s) 893
BseDI CCNNGG 3 cut(s) 126, 460, 675
BseGI GGATG 2 cut(s) 60, 393
BseJI GATNNNNATC 2 cut(s) 443, 944
BseLI CCNNNNNNNGG 3 cut(s) 93, 427, 694
BseNI ACTGG 3 cut(s) 98, 109, 336
BseRI GAGGAG 1 cut(s) 274
BseXI GCAGC 1 cut(s) 136
Bsh1236I CGCG 1 cut(s) 502
BshFI GGCC 3 cut(s) 335, 431, 479
BshNI GGYRCC 1 cut(s) 96
BshVI ATCGAT 1 cut(s) 893
BsiHKAI GWGCWC 2 cut(s) 329, 570
BsiSI CCGG 1 cut(s) 765
BslI CCNNNNNNNGG 3 cut(s) 93, 427, 694
BsmAI GTCTC 2 cut(s) 347, 523
BsnI GGCC 3 cut(s) 335, 431, 479
Bso31I GGTCTC 1 cut(s) 523
Bsp1286I GDGCHC 2 cut(s) 329, 570
Bsp143I GATC 8 cut(s) 201, 309, 397, 444, 484, 775, 890, 894
BspACI CCGC 2 cut(s) 205, 502
BspANI GGCC 3 cut(s) 335, 431, 479
BspDI ATCGAT 1 cut(s) 893
BspFNI CGCG 1 cut(s) 502
BspLI GGNNCC 3 cut(s) 98, 302, 681
BspPI GGATC 3 cut(s) 196, 317, 405
BspT107I GGYRCC 1 cut(s) 96
BspTNI GGTCTC 1 cut(s) 523
BsrI ACTGG 3 cut(s) 98, 109, 336
BssECI CCNNGG 3 cut(s) 126, 460, 675
BssMI GATC 8 cut(s) 201, 309, 397, 444, 484, 775, 890, 894
BssT1I CCWWGG 2 cut(s) 126, 675
Bst2UI CCWGG 2 cut(s) 428, 644
Bst4CI ACNGT 1 cut(s) 733
Bst6I CTCTTC 2 cut(s) 230, 338
BstC8I GCNNGC 1 cut(s) 795
BstDSI CCRYGG 1 cut(s) 460
BstF5I GGATG 2 cut(s) 60, 393
BstFNI CGCG 1 cut(s) 502
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 8 cut(s) 204, 312, 400, 447, 487, 778, 893, 897
BstMAI GTCTC 2 cut(s) 347, 523
BstMBI GATC 8 cut(s) 201, 309, 397, 444, 484, 775, 890, 894
BstMWI GCNNNNNNNGC 3 cut(s) 55, 204, 671
BstNI CCWGG 2 cut(s) 428, 644
BstNSI RCATGY 1 cut(s) 743
BstSCI CCNGG 3 cut(s) 426, 642, 764
BstUI CGCG 1 cut(s) 502
BstV1I GCAGC 1 cut(s) 136
BstX2I RGATCY 2 cut(s) 201, 309
BstYI RGATCY 2 cut(s) 201, 309
Bsu15I ATCGAT 1 cut(s) 893
BsuI GTATCC 3 cut(s) 480, 619, 721
BsuRI GGCC 3 cut(s) 335, 431, 479
BsuTUI ATCGAT 1 cut(s) 893
BtgI CCRYGG 1 cut(s) 460
BtsCI GGATG 2 cut(s) 60, 393
BtsIMutI CAGTG 2 cut(s) 102, 343
Cac8I GCNNGC 1 cut(s) 795
CfoI GCGC 1 cut(s) 502
ClaI ATCGAT 1 cut(s) 893
CsiI ACCWGGT 1 cut(s) 642
Csp6I GTAC 3 cut(s) 97, 319, 505
CspCI CAANNNNNGTGG 2 cut(s) 528, 563
CviAII CATG 6 cut(s) 14, 88, 322, 415, 433, 740
CviJI RGCY 9 cut(s) 149, 265, 303, 327, 335, 431, 479, 606, 674
CviKI_1 RGCY 9 cut(s) 149, 265, 303, 327, 335, 431, 479, 606, 674
CviQI GTAC 3 cut(s) 97, 319, 505
DpnI GATC 8 cut(s) 203, 311, 399, 446, 486, 777, 892, 896
DpnII GATC 8 cut(s) 201, 309, 397, 444, 484, 775, 890, 894
DraI TTTAAA 1 cut(s) 33
EaeI YGGCCR 1 cut(s) 477
Eam1104I CTCTTC 2 cut(s) 230, 338
EarI CTCTTC 2 cut(s) 230, 338
EciI GGCGGA 1 cut(s) 194
Ecl136II GAGCTC 1 cut(s) 327
Eco130I CCWWGG 2 cut(s) 126, 675
Eco24I GRGCYC 1 cut(s) 329
Eco31I GGTCTC 1 cut(s) 523
Eco53kI GAGCTC 1 cut(s) 327
EcoICRI GAGCTC 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 926
EcoRII CCWGG 2 cut(s) 426, 642
EcoT14I CCWWGG 2 cut(s) 126, 675
EcoT38I GRGCYC 1 cut(s) 329
ErhI CCWWGG 2 cut(s) 126, 675
FaeI CATG 6 cut(s) 17, 91, 325, 418, 436, 743
FalI AAGNNNNNCTT 2 cut(s) 505, 537
FatI CATG 6 cut(s) 13, 87, 321, 414, 432, 739
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 1 cut(s) 484
FblI GTMKAC 1 cut(s) 882
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 2 cut(s) 67, 400
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 1 cut(s) 976
GlaI GCGC 1 cut(s) 501
GluI GCNGC 1 cut(s) 150
HaeIII GGCC 3 cut(s) 335, 431, 479
HapII CCGG 1 cut(s) 765
HhaI GCGC 1 cut(s) 502
Hin1II CATG 6 cut(s) 17, 91, 325, 418, 436, 743
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HinfI GANTC 5 cut(s) 240, 355, 803, 956, 970
HpaII CCGG 1 cut(s) 765
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 3 cut(s) 291, 507, 883
Hpy188I TCNGA 3 cut(s) 245, 489, 577
Hpy188III TCNNGA 6 cut(s) 307, 448, 526, 616, 695, 807
Hpy8I GTNNAC 3 cut(s) 291, 507, 883
Hpy99I CGWCG 1 cut(s) 478
HpyAV CCTTC 3 cut(s) 343, 693, 763
HpyCH4III ACNGT 1 cut(s) 733
HpyCH4IV ACGT 2 cut(s) 251, 786
HpyCH4V TGCA 5 cut(s) 362, 394, 722, 797, 910
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 204, 671
HpySE526I ACGT 2 cut(s) 251, 786
Hsp92II CATG 6 cut(s) 17, 91, 325, 418, 436, 743
HspAI GCGC 1 cut(s) 500
KpnI GGTACC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 8 cut(s) 201, 309, 397, 444, 484, 775, 890, 894
LmnI GCTCC 4 cut(s) 300, 573, 712, 832
Lsp1109I GCAGC 1 cut(s) 136
LweI GCATC 2 cut(s) 45, 52
MabI ACCWGGT 1 cut(s) 642
MaeI CTAG 1 cut(s) 976
MaeII ACGT 2 cut(s) 251, 786
MaeIII GTNAC 2 cut(s) 115, 971
MalI GATC 8 cut(s) 203, 311, 399, 446, 486, 777, 892, 896
MboI GATC 8 cut(s) 201, 309, 397, 444, 484, 775, 890, 894
MboII GAAGA 4 cut(s) 247, 355, 710, 785
MfeI CAATTG 1 cut(s) 294
MflI RGATCY 2 cut(s) 201, 309
MhlI GDGCHC 2 cut(s) 329, 570
MluCI AATT 5 cut(s) 81, 294, 587, 690, 926
MlyI GAGTC 2 cut(s) 249, 349
MroXI GAANNNNTTC 1 cut(s) 926
MseI TTAA 3 cut(s) 32, 405, 843
MslI CAYNNNNRTG 1 cut(s) 654
MspI CCGG 1 cut(s) 765
MspR9I CCNGG 3 cut(s) 428, 644, 766
MunI CAATTG 1 cut(s) 294
MvaI CCWGG 2 cut(s) 428, 644
MvnI CGCG 1 cut(s) 502
MwoI GCNNNNNNNGC 3 cut(s) 55, 204, 671
NciI CCSGG 1 cut(s) 766
NdeI CATATG 1 cut(s) 553
NdeII GATC 8 cut(s) 201, 309, 397, 444, 484, 775, 890, 894
NlaIII CATG 6 cut(s) 17, 91, 325, 418, 436, 743
NlaIV GGNNCC 3 cut(s) 98, 302, 681
NmuCI GTSAC 2 cut(s) 115, 971
NspI RCATGY 1 cut(s) 743
PciI ACATGT 1 cut(s) 739
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 2 cut(s) 803, 956
PflMI CCANNNNNTGG 1 cut(s) 93
PkrI GCNGC 1 cut(s) 151
PleI GAGTC 2 cut(s) 248, 349
PpsI GAGTC 2 cut(s) 248, 349
PscI ACATGT 1 cut(s) 739
Psp124BI GAGCTC 1 cut(s) 329
Psp6I CCWGG 2 cut(s) 426, 642
PspGI CCWGG 2 cut(s) 426, 642
PspN4I GGNNCC 3 cut(s) 98, 302, 681
PsuI RGATCY 2 cut(s) 201, 309
RsaI GTAC 3 cut(s) 98, 320, 506
RsaNI GTAC 3 cut(s) 97, 319, 505
RseI CAYNNNNRTG 1 cut(s) 654
SacI GAGCTC 1 cut(s) 329
SaqAI TTAA 3 cut(s) 32, 405, 843
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 8 cut(s) 201, 309, 397, 444, 484, 775, 890, 894
SchI GAGTC 2 cut(s) 249, 349
ScrFI CCNGG 3 cut(s) 428, 644, 766
SduI GDGCHC 2 cut(s) 329, 570
SexAI ACCWGGT 1 cut(s) 642
SfaNI GCATC 2 cut(s) 45, 52
SmiMI CAYNNNNRTG 1 cut(s) 654
Sse9I AATT 5 cut(s) 81, 294, 587, 690, 926
SsiI CCGC 2 cut(s) 205, 502
SspMI CTAG 1 cut(s) 976
SstI GAGCTC 1 cut(s) 329
StyD4I CCNGG 3 cut(s) 426, 642, 764
StyI CCWWGG 2 cut(s) 126, 675
TaaI ACNGT 1 cut(s) 733
TaiI ACGT 2 cut(s) 254, 789
TaqI TCGA 7 cut(s) 233, 437, 449, 525, 633, 778, 893
TasI AATT 5 cut(s) 81, 294, 587, 690, 926
TfiI GAWTC 2 cut(s) 803, 956
Tru1I TTAA 3 cut(s) 32, 405, 843
Tru9I TTAA 3 cut(s) 32, 405, 843
TscAI CASTG 2 cut(s) 109, 343
TseFI GTSAC 2 cut(s) 115, 971
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 2 cut(s) 115, 971
TspDTI ATGAA 5 cut(s) 104, 203, 431, 638, 952
TspGWI ACGGA 2 cut(s) 462, 477
TspRI CASTG 2 cut(s) 109, 343
Van91I CCANNNNNTGG 1 cut(s) 93
XapI RAATTY 1 cut(s) 926
XceI RCATGY 1 cut(s) 743
XmiI GTMKAC 1 cut(s) 882
XmnI GAANNNNTTC 1 cut(s) 926
XspI CTAG 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.