pycom16g12540

peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0022367
Physical Location & Seq
Forward (+)
8829261 .. 8830417
1157 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
ATGATCGGATTTTCGGGGTTTGGCTCGGTGGCGTTGAGCTTCTCAGGAGCTACACGGCTGAGTCAACGCAGAATGGGATTGTTTGGAGTGTCAAGACGGACGTCACAAGATAAAACATGCACAGGGATTTACCAAGTGAATATTAGCATCCATTTTTACCCTGCTGAGAAAAATTTGAATTATAACTATGAGCAAAATTTGGGAAATTTGGATTCTGGGTACCATTATTGGGCGGATTTGATCTTGCCCATTTCGAGAAATCTGCCTTTGAATGATGGGTTGTGGTTTGAAATCCATTATTCGACCGATACACAGTTGAAGGAATTTAAGATTCTTCAGAATAATGATGAGTTTTGGTATTCAAATGCTCCGAATGAGTACATTGCTGTAAACAATCTTAGAGGTACACCTGGAAACGGGCCTTTTAGGGAGGTTGTGGTGAGTTTAGATGGTAAGGTTGTTGGTGCAATCTGGCCTTTTACTGTGATTTTCACTGGAGGGTTCAATCCCCTCCTGTGGAGTCCAATTACTGTGATTGGCTCGTACAATCTCCCTTCTTATGATATTGAAATCACGTCCTTTTTAGGGAAGATATTAGATGGGAAGAGCCACACGTTTGGTTTTAGTGTCACAAATGTGTTGAATATTTGGTGTGTTGACGCGAATTTGCATCTTTGGTTGGACAAGCGGAGCACAAAAACTGAAGGAGGGCTTTTGGAGCATAGTAGCTTGCCCCTTGTTGTTTCATTGGTTTTGGATTTCAAGGGTTTAAATGGCACATTTTTGACAAGCGTCGGCAGGTCTGTGTCATCGACTGGATGGGTGAAGTCCTCCTTTGGGAATATCACAACTGATGCAACTCAAGACCTCCATTATGGTAATACAATGGTCATCAAGAATGATGGCAATACGCAGATAGTGAATCAGACAATCCATTTCAACGATACAGTTCATATCAGGCCGCCATCCTCCATTATCCACTCAATGTCATCAAACGAAAAATTTCCTCTTTACTTTACATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

342

Amino Acids

38.07

Weight (kDa)

6.35

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 37 - 240 1.8e-43 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C PF25156 260 - 337 9.2e-22 PNGase_A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022367)

Species Orthologous Gene IDs
malus_domestica MD08G1248900.v1.1 MD16G1147200.v1.1
pyrus_communis pycom16g12540 pycom16g12550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 183, 1024
AatII GACGTC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 789
Acc65I GGTACC 1 cut(s) 219
AccB1I GGYRCC 1 cut(s) 219
AccII CGCG 1 cut(s) 662
AciI CCGC 3 cut(s) 233, 688, 962
AcsI RAATTY 6 cut(s) 172, 196, 205, 323, 664, 1001
AcuI CTGAAG 2 cut(s) 320, 723
AcyI GRCGYC 1 cut(s) 101
AfaI GTAC 4 cut(s) 221, 380, 406, 545
AfiI CCNNNNNNNGG 5 cut(s) 229, 416, 516, 585, 837
AflIII ACRYGT 1 cut(s) 612
AjiI CACGTC 1 cut(s) 576
AjnI CCWGG 1 cut(s) 409
AleI CACNNNNGTG 1 cut(s) 635
AluBI AGCT 3 cut(s) 39, 50, 729
AluI AGCT 3 cut(s) 39, 50, 729
Alw21I GWGCWC 1 cut(s) 695
AoxI GGCC 3 cut(s) 419, 473, 959
ApoI RAATTY 6 cut(s) 172, 196, 205, 323, 664, 1001
Asp700I GAANNNNTTC 1 cut(s) 1002
Asp718I GGTACC 1 cut(s) 219
AspS9I GGNCC 1 cut(s) 419
AsuHPI GGTGA 2 cut(s) 451, 835
BanI GGYRCC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 695
BccI CCATC 6 cut(s) 269, 443, 593, 813, 896, 973
BceAI ACGGC 1 cut(s) 71
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 1 cut(s) 411
BfuAI ACCTGC 1 cut(s) 789
BisI GCNGC 1 cut(s) 962
BlsI GCNGC 1 cut(s) 963
Bme1390I CCNGG 1 cut(s) 411
BmgBI CACGTC 1 cut(s) 576
BmgT120I GGNCC 1 cut(s) 419
BmiI GGNNCC 1 cut(s) 221
BmrFI CCNGG 1 cut(s) 411
BmsI GCATC 3 cut(s) 156, 679, 844
BoxI GACNNNNGTC 2 cut(s) 100, 791
BpmI CTGGAG 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 846
BsaHI GRCGYC 1 cut(s) 101
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 416, 516, 585, 837
Bse1I ACTGG 2 cut(s) 499, 820
Bse3DI GCAATG 1 cut(s) 381
BseBI CCWGG 1 cut(s) 411
BseGI GGATG 3 cut(s) 147, 824, 965
BseLI CCNNNNNNNGG 5 cut(s) 229, 416, 516, 585, 837
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 3 cut(s) 50, 57, 156
BseNI ACTGG 2 cut(s) 499, 820
Bsh1236I CGCG 1 cut(s) 662
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 3 cut(s) 421, 475, 961
BshNI GGYRCC 1 cut(s) 219
BsiEI CGRYCG 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 695
BslI CCNNNNNNNGG 5 cut(s) 229, 416, 516, 585, 837
BsnI GGCC 3 cut(s) 421, 475, 961
Bsp1286I GDGCHC 1 cut(s) 695
Bsp143I GATC 2 cut(s) 3, 240
BspACI CCGC 3 cut(s) 233, 688, 962
BspANI GGCC 3 cut(s) 421, 475, 961
BspCNI CTCAG 3 cut(s) 51, 56, 157
BspFNI CGCG 1 cut(s) 662
BspLI GGNNCC 1 cut(s) 221
BspMI ACCTGC 1 cut(s) 789
BspQI GCTCTTC 1 cut(s) 599
BspT107I GGYRCC 1 cut(s) 219
BsrDI GCAATG 1 cut(s) 381
BsrI ACTGG 2 cut(s) 499, 820
BssMI GATC 2 cut(s) 3, 240
BssNI GRCGYC 1 cut(s) 101
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 4 cut(s) 315, 484, 532, 949
Bst6I CTCTTC 1 cut(s) 599
BstACI GRCGYC 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 731
BstDEI CTNAG 4 cut(s) 43, 59, 165, 398
BstF5I GGATG 3 cut(s) 147, 824, 965
BstFNI CGCG 1 cut(s) 662
BstKTI GATC 2 cut(s) 6, 243
BstMBI GATC 2 cut(s) 3, 240
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 1 cut(s) 718
BstNI CCWGG 1 cut(s) 411
BstNSI RCATGY 1 cut(s) 120
BstPAI GACNNNNGTC 2 cut(s) 100, 791
BstSCI CCNGG 1 cut(s) 409
BstUI CGCG 1 cut(s) 662
BstXI CCANNNNNNTGG 1 cut(s) 617
BsuRI GGCC 3 cut(s) 421, 475, 961
BtrI CACGTC 1 cut(s) 576
BtsCI GGATG 3 cut(s) 147, 824, 965
BtsIMutI CAGTG 1 cut(s) 492
BveI ACCTGC 1 cut(s) 789
Cac8I GCNNGC 1 cut(s) 731
Cfr13I GGNCC 1 cut(s) 419
CseI GACGC 2 cut(s) 668, 781
Csp6I GTAC 4 cut(s) 220, 379, 405, 544
CviAII CATG 1 cut(s) 117
CviQI GTAC 4 cut(s) 220, 379, 405, 544
DdeI CTNAG 4 cut(s) 43, 59, 165, 398
DpnI GATC 2 cut(s) 5, 242
DpnII GATC 2 cut(s) 3, 240
DraI TTTAAA 1 cut(s) 771
Eam1104I CTCTTC 1 cut(s) 599
EarI CTCTTC 1 cut(s) 599
EciI GGCGGA 1 cut(s) 248
Eco57I CTGAAG 2 cut(s) 320, 723
EcoRII CCWGG 1 cut(s) 409
FaeI CATG 1 cut(s) 120
FaiI YATR 8 cut(s) 118, 183, 189, 561, 723, 876, 954, 1024
FalI AAGNNNNNCTT 4 cut(s) 696, 728, 818, 850
FatI CATG 1 cut(s) 116
Fnu4HI GCNGC 1 cut(s) 962
FokI GGATG 3 cut(s) 134, 831, 952
Fsp4HI GCNGC 1 cut(s) 962
GluI GCNGC 1 cut(s) 962
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 3 cut(s) 421, 475, 961
HgaI GACGC 2 cut(s) 668, 781
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 1 cut(s) 120
HincII GTYRAC 2 cut(s) 65, 658
HindII GTYRAC 2 cut(s) 65, 658
HinfI GANTC 5 cut(s) 61, 212, 331, 520, 922
HphI GGTGA 2 cut(s) 451, 835
Hpy166II GTNNAC 4 cut(s) 65, 391, 407, 658
Hpy188I TCNGA 4 cut(s) 8, 339, 372, 927
Hpy188III TCNNGA 5 cut(s) 45, 93, 255, 863, 895
Hpy8I GTNNAC 4 cut(s) 65, 391, 407, 658
Hpy99I CGWCG 1 cut(s) 797
HpyAV CCTTC 3 cut(s) 313, 564, 698
HpyCH4III ACNGT 4 cut(s) 315, 484, 532, 949
HpyCH4IV ACGT 3 cut(s) 101, 575, 614
HpyCH4V TGCA 4 cut(s) 120, 467, 670, 857
HpyF10VI GCNNNNNNNGC 1 cut(s) 718
HpyF3I CTNAG 4 cut(s) 43, 59, 165, 398
HpySE526I ACGT 3 cut(s) 101, 575, 614
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 1 cut(s) 120
KpnI GGTACC 1 cut(s) 223
Kzo9I GATC 2 cut(s) 3, 240
LguI GCTCTTC 1 cut(s) 599
LmnI GCTCC 4 cut(s) 47, 373, 690, 718
LweI GCATC 3 cut(s) 156, 679, 844
MaeII ACGT 3 cut(s) 101, 575, 614
MaeIII GTNAC 2 cut(s) 102, 628
MalI GATC 2 cut(s) 5, 242
MboI GATC 2 cut(s) 3, 240
MboII GAAGA 3 cut(s) 326, 601, 616
MhlI GDGCHC 1 cut(s) 695
MluCI AATT 8 cut(s) 172, 178, 196, 205, 323, 525, 664, 1001
MlyI GAGTC 2 cut(s) 70, 529
MmeI TCCRAC 1 cut(s) 660
MnlI CCTC 9 cut(s) 395, 424, 491, 521, 701, 841, 878, 979, 1017
MroXI GAANNNNTTC 1 cut(s) 1002
MseI TTAA 2 cut(s) 327, 770
MslI CAYNNNNRTG 1 cut(s) 635
MspR9I CCNGG 1 cut(s) 411
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 1 cut(s) 662
MwoI GCNNNNNNNGC 1 cut(s) 718
NdeII GATC 2 cut(s) 3, 240
NlaIII CATG 1 cut(s) 120
NlaIV GGNNCC 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 102, 628
NspI RCATGY 1 cut(s) 120
OliI CACNNNNGTG 1 cut(s) 635
PciSI GCTCTTC 1 cut(s) 599
PdmI GAANNNNTTC 1 cut(s) 1002
PfeI GAWTC 3 cut(s) 212, 331, 922
PkrI GCNGC 1 cut(s) 963
PleI GAGTC 2 cut(s) 69, 528
PpsI GAGTC 2 cut(s) 69, 528
PshAI GACNNNNGTC 2 cut(s) 100, 791
PsiI TTATAA 2 cut(s) 183, 1024
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 221
PspPI GGNCC 1 cut(s) 419
RsaI GTAC 4 cut(s) 221, 380, 406, 545
RsaNI GTAC 4 cut(s) 220, 379, 405, 544
RseI CAYNNNNRTG 1 cut(s) 635
SapI GCTCTTC 1 cut(s) 599
SaqAI TTAA 2 cut(s) 327, 770
SatI GCNGC 1 cut(s) 962
Sau3AI GATC 2 cut(s) 3, 240
Sau96I GGNCC 1 cut(s) 419
SchI GAGTC 2 cut(s) 70, 529
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 1 cut(s) 695
SfaNI GCATC 3 cut(s) 156, 679, 844
SmiMI CAYNNNNRTG 1 cut(s) 635
SmlI CTYRAG 1 cut(s) 861
SmoI CTYRAG 1 cut(s) 861
Sse9I AATT 8 cut(s) 172, 178, 196, 205, 323, 525, 664, 1001
SsiI CCGC 3 cut(s) 233, 688, 962
SspI AATATT 2 cut(s) 142, 646
StyD4I CCNGG 1 cut(s) 409
TaaI ACNGT 4 cut(s) 315, 484, 532, 949
TaiI ACGT 3 cut(s) 104, 578, 617
TaqI TCGA 3 cut(s) 254, 302, 812
TaqII GACCGA 1 cut(s) 320
TasI AATT 8 cut(s) 172, 178, 196, 205, 323, 525, 664, 1001
TatI WGTACW 1 cut(s) 378
TauI GCSGC 1 cut(s) 964
TfiI GAWTC 3 cut(s) 212, 331, 922
Tru1I TTAA 2 cut(s) 327, 770
Tru9I TTAA 2 cut(s) 327, 770
TscAI CASTG 1 cut(s) 499
TseFI GTSAC 2 cut(s) 102, 628
Tsp45I GTSAC 2 cut(s) 102, 628
TspDTI ATGAA 2 cut(s) 735, 941
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 1 cut(s) 499
XapI RAATTY 6 cut(s) 172, 196, 205, 323, 664, 1001
XceI RCATGY 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 1002
ZraI GACGTC 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.