pycom16g14920

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0021841
Physical Location & Seq
Reverse (-)
10925346 .. 10926872
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1407 bp
ATGGTCTGGGAGAAGGGCATATTGCCCGACGTGGCCATCCACACTGTCGTGATTGAGGCCTACACCAATGCCGGGAAGGCTGAGGGTGCTCTCAAGGCTTACAAGCACATGTTGGCCAATGGGATCAAACCCAATTTATATTCTTACAGCATTCTCATCCAGGCACTGGCTGCTGAACCGGATTTCATTCAGGATGCCAGGAAATACTTTCTTGTGATGATGGATAACGGAACATGGCCCAATGACGATACTCTCAAAGCCGTGACCAAGGGAGCTGATTCAGAGGACAATGAGTACATGGAGTTTGCTAAGGCTGTGAGCGCTAGGGGGTCACAAATCTGCTACAAGAATACTGCGATTTTGATACTTCGAGAGAATATAGTTGAAGCACTGAAGTTTTTTTTTTCTCCTTCCATTACATTATCAGGCAAAGAAGAAGAAGACCCGGAAGACATACAAGAAATCTTGCACAGATTAGAATGTGGGATACCCGTTAAGGCTGAAACATACAATATGGTCATCAAGAGACTCGCTTTCCAGAAACCAGACCCCGTTGCCATTAAGTGTGCCAAGAAGTACTTTCTGCAAATGCTTGACAAAGGAATGGAGCCTGATCCCCCTGCCTACACCTTCGTGATGGAGGCCATTGCCTGCCAGATGGACGATAAGGAGAAGGTGGTGGCGGAGGGCAGGGAGTTTGTGAAGCAAATTAAACATGAGGGGTTTGATCTTGAAAATCAAAAGTACTTTGAATACCACAAACACCTTGAAGAGGCATTCGATGCATATAGGATGTCCGACTATGCCACCAAAACAACTGATGAGGAGATGCGAAAAACCTTCTTCAGGAAACATATAATGGATATGGTCGAAGGTATGGTAGATGACGGCTTTAAGGATGAGGTCAAGGAGCTCTTTGAGCCGTTCTTTGACACAGGCGTACTACCCGCCATTGTCACCCACACCGCTGTCATTGAAGCCTATGCCACTGCCGGCAAGATTAAGGGTGCTCTTGAGGCTTATCAACGCATGTCGGCGGTTGGGGTTATGCCGAACTGCTACACTTATAGTGTCTTCATCAAGGCACTTGCTACTGACCCCAACTTCTTTGGGGATGCCAAGAAGTATTTGCTGGAGATGATGGAGAAGGGGATGCATCCCAATGCTGCTACCTACACCACAGTTTTTGAGGGCTTCGCGAGGCAAGGGGACAGGGCGGTAGAGGACGGGAATGAGTTTTTACAGGTAATGAAAACCAAGGGATTTGTGCCGGACGAGGAGGCTGTGGGTGAGGTTCTGAAAGGACGAACTGGAGAAGGGGTTAGCTGCGTCATGAACATTCTGTTTGGCAGAATAACTTGTAGTGAAACCGAAATTCATGCATGTCATAGTACCGAATATCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

469

Amino Acids

52.9

Weight (kDa)

5.08

Isoelectric Point (pI)

33.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 4 - 56 1.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 9 - 57 1.5e-11 PPR repeat family
PPR_3 PF13812 311 - 363 1.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 316 - 364 1.5e-10 PPR repeat family
TPR_24 PF23276 321 - 404 6.2e-08 Fungal tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021841)

Species Orthologous Gene IDs
malus_domestica MD16G1177800.v1.1 MD16G1193600.v1.1
pyrus_communis pycom16g14920 pycom16g16330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 166
AccII CGCG 1 cut(s) 1199
AciI CCGC 5 cut(s) 683, 948, 966, 1037, 1217
AclWI GGATC 2 cut(s) 131, 608
AcoI YGGCCR 2 cut(s) 33, 114
AcsI RAATTY 1 cut(s) 1374
AcuI CTGAAG 2 cut(s) 413, 829
AfaI GTAC 5 cut(s) 296, 578, 746, 942, 1393
AfeI AGCGCT 1 cut(s) 322
AfiI CCNNNNNNNGG 4 cut(s) 72, 166, 772, 846
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 5 cut(s) 386, 734, 752, 770, 977
AjiI CACGTC 1 cut(s) 31
AjnI CCWGG 2 cut(s) 159, 197
AleI CACNNNNGTG 2 cut(s) 47, 632
AluBI AGCT 3 cut(s) 275, 913, 1326
AluI AGCT 3 cut(s) 275, 913, 1326
Alw21I GWGCWC 3 cut(s) 91, 915, 1012
Alw26I GTCTC 1 cut(s) 520
AlwI GGATC 2 cut(s) 131, 608
AlwNI CAGNNNCTG 1 cut(s) 166
Aor51HI AGCGCT 1 cut(s) 322
AoxI GGCC 5 cut(s) 33, 57, 114, 236, 642
ApeKI GCWGC 3 cut(s) 170, 1166, 1326
ApoI RAATTY 1 cut(s) 1374
AspLEI GCGC 1 cut(s) 323
AspS9I GGNCC 1 cut(s) 237
AsuC2I CCSGG 2 cut(s) 73, 446
AsuHPI GGTGA 2 cut(s) 949, 1301
BalI TGGCCA 2 cut(s) 35, 116
BanII GRGCYC 1 cut(s) 915
BbsI GAAGAC 3 cut(s) 447, 456, 1066
Bbv12I GWGCWC 3 cut(s) 91, 915, 1012
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 3 cut(s) 157, 1153, 1313
BccI CCATC 5 cut(s) 44, 214, 631, 652, 1135
BceAI ACGGC 3 cut(s) 245, 904, 907
BcgI CGANNNNNNTGC 2 cut(s) 1361, 1395
BciT130I CCWGG 2 cut(s) 161, 199
BciVI GTATCC 1 cut(s) 480
BcnI CCSGG 2 cut(s) 73, 446
BcoDI GTCTC 1 cut(s) 520
BfaI CTAG 1 cut(s) 324
BfoI RGCGCY 1 cut(s) 324
BfuI GTATCC 1 cut(s) 480
BglI GCCNNNNNGGC 1 cut(s) 77
BisI GCNGC 3 cut(s) 171, 1167, 1327
BlsI GCNGC 3 cut(s) 172, 1168, 1328
BmcAI AGTACT 2 cut(s) 578, 746
Bme1390I CCNGG 4 cut(s) 73, 161, 199, 446
BmgBI CACGTC 1 cut(s) 31
BmgT120I GGNCC 1 cut(s) 237
BmiI GGNNCC 1 cut(s) 609
BmrFI CCNGG 4 cut(s) 73, 161, 199, 446
BmsI GCATC 6 cut(s) 184, 772, 819, 1105, 1143, 1165
BpiI GAAGAC 3 cut(s) 447, 456, 1066
BpmI CTGGAG 2 cut(s) 1154, 1332
Bpu10I CCTNAGC 2 cut(s) 81, 309
BpuEI CTTGAG 2 cut(s) 77, 1034
BpuMI CCSGG 2 cut(s) 73, 446
BsaJI CCNNGG 2 cut(s) 267, 1257
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 2 cut(s) 1305, 1335
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 166, 772, 846
Bse118I RCCGGY 1 cut(s) 992
Bse1I ACTGG 2 cut(s) 171, 1315
Bse3DI GCAATG 1 cut(s) 645
BseBI CCWGG 2 cut(s) 161, 199
BseDI CCNNGG 2 cut(s) 267, 1257
BseGI GGATG 8 cut(s) 36, 156, 199, 798, 904, 1120, 1156, 1158
BseLI CCNNNNNNNGG 4 cut(s) 72, 166, 772, 846
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 171, 1315
BseRI GAGGAG 2 cut(s) 839, 1292
BseXI GCAGC 3 cut(s) 157, 1153, 1313
Bsh1236I CGCG 1 cut(s) 1199
BshFI GGCC 5 cut(s) 35, 59, 116, 238, 644
BsiHKAI GWGCWC 3 cut(s) 91, 915, 1012
BsiSI CCGG 5 cut(s) 72, 179, 446, 993, 1271
BslFI GGGAC 1 cut(s) 1223
BslI CCNNNNNNNGG 4 cut(s) 72, 166, 772, 846
BsmAI GTCTC 1 cut(s) 520
BsmFI GGGAC 1 cut(s) 1223
BsmI GAATGC 2 cut(s) 150, 776
BsnI GGCC 5 cut(s) 35, 59, 116, 238, 644
Bsp1286I GDGCHC 3 cut(s) 91, 915, 1012
Bsp143I GATC 3 cut(s) 123, 613, 727
Bsp68I TCGCGA 1 cut(s) 1199
BspACI CCGC 5 cut(s) 683, 948, 966, 1037, 1217
BspANI GGCC 5 cut(s) 35, 59, 116, 238, 644
BspCNI CTCAG 1 cut(s) 73
BspFNI CGCG 1 cut(s) 1199
BspHI TCATGA 1 cut(s) 1332
BspLI GGNNCC 1 cut(s) 609
BspPI GGATC 2 cut(s) 131, 608
BsrDI GCAATG 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 992
BsrI ACTGG 2 cut(s) 171, 1315
BssAI RCCGGY 1 cut(s) 992
BssECI CCNNGG 2 cut(s) 267, 1257
BssMI GATC 3 cut(s) 123, 613, 727
BssT1I CCWWGG 2 cut(s) 267, 1257
Bst2UI CCWGG 2 cut(s) 161, 199
Bst4CI ACNGT 2 cut(s) 46, 1183
Bst6I CTCTTC 1 cut(s) 765
BstAPI GCANNNNNTGC 2 cut(s) 170, 782
BstC8I GCNNGC 2 cut(s) 652, 994
BstDEI CTNAG 2 cut(s) 81, 309
BstENI CCTNNNNNAGG 2 cut(s) 770, 844
BstF5I GGATG 8 cut(s) 36, 156, 199, 798, 904, 1120, 1156, 1158
BstFNI CGCG 1 cut(s) 1199
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 1 cut(s) 323
BstKTI GATC 3 cut(s) 126, 616, 730
BstMAI GTCTC 1 cut(s) 520
BstMBI GATC 3 cut(s) 123, 613, 727
BstMWI GCNNNNNNNGC 8 cut(s) 77, 86, 95, 170, 320, 782, 919, 1016
BstNI CCWGG 2 cut(s) 161, 199
BstNSI RCATGY 3 cut(s) 112, 1033, 1386
BstSCI CCNGG 4 cut(s) 71, 159, 197, 444
BstUI CGCG 1 cut(s) 1199
BstV1I GCAGC 3 cut(s) 157, 1153, 1313
BstV2I GAAGAC 3 cut(s) 447, 456, 1066
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 5 cut(s) 35, 59, 116, 238, 644
BtrI CACGTC 1 cut(s) 31
BtsCI GGATG 8 cut(s) 36, 156, 199, 798, 904, 1120, 1156, 1158
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 4 cut(s) 42, 164, 389, 987
BtuMI TCGCGA 1 cut(s) 1199
Cac8I GCNNGC 2 cut(s) 652, 994
CaiI CAGNNNCTG 1 cut(s) 166
CciI TCATGA 1 cut(s) 1332
CfoI GCGC 1 cut(s) 323
Cfr10I RCCGGY 1 cut(s) 992
Cfr13I GGNCC 1 cut(s) 237
CseI GACGC 1 cut(s) 1318
Csp6I GTAC 5 cut(s) 295, 577, 745, 941, 1392
CspCI CAANNNNNGTGG 2 cut(s) 1168, 1203
CviAII CATG 8 cut(s) 109, 234, 298, 716, 1030, 1333, 1379, 1383
CviQI GTAC 5 cut(s) 295, 577, 745, 941, 1392
DdeI CTNAG 2 cut(s) 81, 309
DpnI GATC 3 cut(s) 125, 615, 729
DpnII GATC 3 cut(s) 123, 613, 727
EaeI YGGCCR 2 cut(s) 33, 114
Eam1104I CTCTTC 1 cut(s) 765
EarI CTCTTC 1 cut(s) 765
EciI GGCGGA 1 cut(s) 698
Ecl136II GAGCTC 1 cut(s) 913
Eco130I CCWWGG 2 cut(s) 267, 1257
Eco147I AGGCCT 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 915
Eco47III AGCGCT 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 913
Eco57I CTGAAG 2 cut(s) 413, 829
EcoICRI GAGCTC 1 cut(s) 913
EcoNI CCTNNNNNAGG 2 cut(s) 770, 844
EcoRII CCWGG 2 cut(s) 159, 197
EcoT14I CCWWGG 2 cut(s) 267, 1257
EcoT22I ATGCAT 3 cut(s) 787, 1158, 1384
EcoT38I GRGCYC 1 cut(s) 915
ErhI CCWWGG 2 cut(s) 267, 1257
FaeI CATG 8 cut(s) 112, 237, 301, 719, 1033, 1336, 1382, 1386
FalI AAGNNNNNCTT 4 cut(s) 563, 595, 899, 931
FaqI GGGAC 1 cut(s) 1223
FatI CATG 8 cut(s) 108, 233, 297, 715, 1029, 1332, 1378, 1382
FauI CCCGC 1 cut(s) 955
Fnu4HI GCNGC 3 cut(s) 171, 1167, 1327
FokI GGATG 8 cut(s) 23, 143, 206, 805, 911, 1127, 1143, 1165
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 3 cut(s) 171, 1167, 1327
FspBI CTAG 1 cut(s) 324
GlaI GCGC 1 cut(s) 322
GluI GCNGC 3 cut(s) 171, 1167, 1327
GsuI CTGGAG 2 cut(s) 1154, 1332
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 5 cut(s) 35, 59, 116, 238, 644
HapII CCGG 5 cut(s) 72, 179, 446, 993, 1271
HgaI GACGC 1 cut(s) 1318
HhaI GCGC 1 cut(s) 323
Hin1II CATG 8 cut(s) 112, 237, 301, 719, 1033, 1336, 1382, 1386
Hin6I GCGC 1 cut(s) 321
HinP1I GCGC 1 cut(s) 321
HinfI GANTC 2 cut(s) 278, 528
HpaII CCGG 5 cut(s) 72, 179, 446, 993, 1271
HphI GGTGA 2 cut(s) 949, 1301
Hpy188I TCNGA 3 cut(s) 283, 799, 1299
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 9 cut(s) 7, 70, 420, 640, 667, 850, 866, 1141, 1310
HpyCH4III ACNGT 2 cut(s) 46, 1183
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 5 cut(s) 469, 586, 785, 1156, 1382
HpyF10VI GCNNNNNNNGC 8 cut(s) 77, 86, 95, 170, 320, 782, 919, 1016
HpyF3I CTNAG 2 cut(s) 81, 309
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 8 cut(s) 112, 237, 301, 719, 1033, 1336, 1382, 1386
HspAI GCGC 1 cut(s) 321
KroI GCCGGC 1 cut(s) 992
KroNI GCCGGC 1 cut(s) 994
Kzo9I GATC 3 cut(s) 123, 613, 727
LmnI GCTCC 3 cut(s) 272, 607, 910
Lsp1109I GCAGC 3 cut(s) 157, 1153, 1313
LweI GCATC 6 cut(s) 184, 772, 819, 1105, 1143, 1165
MaeI CTAG 1 cut(s) 324
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 3 cut(s) 262, 330, 955
MalI GATC 3 cut(s) 125, 615, 729
MboI GATC 3 cut(s) 123, 613, 727
MboII GAAGA 7 cut(s) 446, 449, 452, 461, 782, 835, 1066
MhlI GDGCHC 3 cut(s) 91, 915, 1012
MlsI TGGCCA 2 cut(s) 35, 116
MluCI AATT 3 cut(s) 133, 708, 1374
MluNI TGGCCA 2 cut(s) 35, 116
MlyI GAGTC 1 cut(s) 522
MmeI TCCRAC 1 cut(s) 822
Mox20I TGGCCA 2 cut(s) 35, 116
Mph1103I ATGCAT 3 cut(s) 787, 1158, 1384
MroNI GCCGGC 1 cut(s) 992
MscI TGGCCA 2 cut(s) 35, 116
MseI TTAA 6 cut(s) 495, 561, 711, 894, 1002, 1405
MslI CAYNNNNRTG 3 cut(s) 47, 632, 1161
Msp20I TGGCCA 2 cut(s) 35, 116
MspA1I CMGCKG 1 cut(s) 968
MspI CCGG 5 cut(s) 72, 179, 446, 993, 1271
MspR9I CCNGG 4 cut(s) 73, 161, 199, 446
Mva1269I GAATGC 2 cut(s) 150, 776
MvaI CCWGG 2 cut(s) 161, 199
MvnI CGCG 1 cut(s) 1199
MwoI GCNNNNNNNGC 8 cut(s) 77, 86, 95, 170, 320, 782, 919, 1016
NaeI GCCGGC 1 cut(s) 994
NciI CCSGG 2 cut(s) 73, 446
NdeII GATC 3 cut(s) 123, 613, 727
NgoMIV GCCGGC 1 cut(s) 992
NlaIII CATG 8 cut(s) 112, 237, 301, 719, 1033, 1336, 1382, 1386
NlaIV GGNNCC 1 cut(s) 609
NmuCI GTSAC 3 cut(s) 262, 330, 955
NruI TCGCGA 1 cut(s) 1199
NsiI ATGCAT 3 cut(s) 787, 1158, 1384
NspI RCATGY 3 cut(s) 112, 1033, 1386
OliI CACNNNNGTG 2 cut(s) 47, 632
PagI TCATGA 1 cut(s) 1332
PceI AGGCCT 1 cut(s) 59
PciI ACATGT 1 cut(s) 108
PctI GAATGC 2 cut(s) 150, 776
PdiI GCCGGC 1 cut(s) 994
PfeI GAWTC 1 cut(s) 278
PflMI CCANNNNNTGG 1 cut(s) 166
PkrI GCNGC 3 cut(s) 172, 1168, 1328
PleI GAGTC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 522
PscI ACATGT 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 915
Psp6I CCWGG 2 cut(s) 159, 197
PspGI CCWGG 2 cut(s) 159, 197
PspN4I GGNNCC 1 cut(s) 609
PspPI GGNCC 1 cut(s) 237
PstNI CAGNNNCTG 1 cut(s) 166
RruI TCGCGA 1 cut(s) 1199
RsaI GTAC 5 cut(s) 296, 578, 746, 942, 1393
RsaNI GTAC 5 cut(s) 295, 577, 745, 941, 1392
RseI CAYNNNNRTG 3 cut(s) 47, 632, 1161
SacI GAGCTC 1 cut(s) 915
SaqAI TTAA 6 cut(s) 495, 561, 711, 894, 1002, 1405
SatI GCNGC 3 cut(s) 171, 1167, 1327
Sau3AI GATC 3 cut(s) 123, 613, 727
Sau96I GGNCC 1 cut(s) 237
ScaI AGTACT 2 cut(s) 578, 746
SchI GAGTC 1 cut(s) 522
ScrFI CCNGG 4 cut(s) 73, 161, 199, 446
SduI GDGCHC 3 cut(s) 91, 915, 1012
SfaNI GCATC 6 cut(s) 184, 772, 819, 1105, 1143, 1165
SmiMI CAYNNNNRTG 3 cut(s) 47, 632, 1161
SmlI CTYRAG 2 cut(s) 92, 1013
SmoI CTYRAG 2 cut(s) 92, 1013
Sse9I AATT 3 cut(s) 133, 708, 1374
SseBI AGGCCT 1 cut(s) 59
SsiI CCGC 5 cut(s) 683, 948, 966, 1037, 1217
SspMI CTAG 1 cut(s) 324
SstI GAGCTC 1 cut(s) 915
StuI AGGCCT 1 cut(s) 59
StyD4I CCNGG 4 cut(s) 71, 159, 197, 444
StyI CCWWGG 2 cut(s) 267, 1257
TaaI ACNGT 2 cut(s) 46, 1183
TaiI ACGT 1 cut(s) 33
TaqI TCGA 3 cut(s) 370, 780, 870
TasI AATT 3 cut(s) 133, 708, 1374
TatI WGTACW 3 cut(s) 294, 576, 744
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 6 cut(s) 495, 561, 711, 894, 1002, 1405
Tru9I TTAA 6 cut(s) 495, 561, 711, 894, 1002, 1405
TscAI CASTG 4 cut(s) 49, 171, 396, 994
TseFI GTSAC 3 cut(s) 262, 330, 955
TseI GCWGC 3 cut(s) 170, 1166, 1326
Tsp45I GTSAC 3 cut(s) 262, 330, 955
TspDTI ATGAA 5 cut(s) 175, 1066, 1265, 1349, 1367
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 4 cut(s) 49, 171, 396, 994
Van91I CCANNNNNTGG 1 cut(s) 166
XagI CCTNNNNNAGG 2 cut(s) 770, 844
XapI RAATTY 1 cut(s) 1374
XceI RCATGY 3 cut(s) 112, 1033, 1386
XspI CTAG 1 cut(s) 324
ZrmI AGTACT 2 cut(s) 578, 746
Zsp2I ATGCAT 3 cut(s) 787, 1158, 1384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.