pycom16g20250

Glucomannan 4-beta-mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0022570
Physical Location & Seq
Reverse (-)
17927943 .. 17928751
809 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g20250.2

Sequence Viewer

Length: 669 bp
ATGGCTGATACCATAACCCAAATTCTGATACCAGAAACGTTTCAGAGAGGGACATATGAATTTGCAGGGCAAATTGGGCTGATATGGGAGCTAATCAAAGCGCCATTGATAGTCCCTCTGCTCACAGTTGGGGTCTATGTCTCATTGGCCATGTCTCTCATGCTCTTCTTTGAGAGGGTTTATATGGGAGTAGTCATCGTTCTCGTCAAACTCTTCTGGAAAAAACCAGAGCAACGCTACAAATACGAGCCCATTCAGGATGACCTTGAACTGGGAAGCTCCAATTTTCCTCCTGTTCTCATCCAAATCCCCATGTTCAATGAAAGAGAGGTCTACAAGGTCTCCATTGGAGCTGCATGTGGACTTTCATGGCCGTCCGATCGTCTCGTGATCCAAGTCCTTGATGATTCAACCGACCCTACAATCAAGGTAAAAAACAATCCTCCCAAATCTCCAATTAAAAAAATCATCCTTTCAATGGGTCCTCTTCCTTTTAGTCCATTAGTTAGAGCTCCAAAGATCTCAATGTGTTGGATAAAATGCCACACTGAAAAAACACCAATAATTGAATGTTTTGGAGCAACCCTTTTGATTTCAGACCCAATTTCTGACATGGGGTTGTTCCAATTTACAATGTGCAAGTGGGGTTTTAATAAAATCGGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_tranf_2_3 Glyco_trans_2_3 Glycos_transf_2

Protein Analysis

223

Amino Acids

25.09

Weight (kDa)

8.53

Isoelectric Point (pI)

31.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022570)

Species Orthologous Gene IDs
pyrus_communis pycom16g20250
rosa_multiflora Rmu_sc0030882.1_g000001 Rmu_sc0038044.1_g000001 Rmu_sc0038045.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 333
AclI AACGTT 1 cut(s) 38
AclWI GGATC 1 cut(s) 385
AcoI YGGCCR 2 cut(s) 147, 371
AcsI RAATTY 2 cut(s) 21, 59
AfiI CCNNNNNNNGG 2 cut(s) 271, 478
AgsI TTSAA 5 cut(s) 269, 319, 411, 477, 569
AluBI AGCT 4 cut(s) 91, 279, 353, 512
AluI AGCT 4 cut(s) 91, 279, 353, 512
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 4 cut(s) 145, 159, 346, 389
AlwI GGATC 1 cut(s) 385
AoxI GGCC 2 cut(s) 147, 371
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 2 cut(s) 21, 59
Asp700I GAANNNNTTC 1 cut(s) 39
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 1 cut(s) 482
AvaII GGWCC 1 cut(s) 482
BalI TGGCCA 1 cut(s) 149
BanII GRGCYC 2 cut(s) 252, 514
BauI CACGAG 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 514
BbvI GCAGC 1 cut(s) 340
BceAI ACGGC 1 cut(s) 358
BcoDI GTCTC 4 cut(s) 145, 159, 346, 389
BfoI RGCGCY 1 cut(s) 104
BglII AGATCT 1 cut(s) 519
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 1 cut(s) 483
BmrI ACTGGG 1 cut(s) 281
BmuI ACTGGG 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 346
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 478
Bse1I ACTGG 1 cut(s) 276
BseGI GGATG 3 cut(s) 265, 300, 468
BseLI CCNNNNNNNGG 2 cut(s) 271, 478
BseNI ACTGG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 340
Bsh1285I CGRYCG 1 cut(s) 382
BshFI GGCC 2 cut(s) 149, 373
BsiEI CGRYCG 1 cut(s) 382
BsiHKAI GWGCWC 1 cut(s) 514
BslFI GGGAC 2 cut(s) 64, 98
BslI CCNNNNNNNGG 2 cut(s) 271, 478
BsmAI GTCTC 4 cut(s) 145, 159, 346, 389
BsmBI CGTCTC 1 cut(s) 389
BsmFI GGGAC 2 cut(s) 64, 98
BsnI GGCC 2 cut(s) 149, 373
Bso31I GGTCTC 1 cut(s) 346
Bsp1286I GDGCHC 2 cut(s) 252, 514
Bsp143I GATC 3 cut(s) 379, 390, 519
BspANI GGCC 2 cut(s) 149, 373
BspLI GGNNCC 1 cut(s) 483
BspPI GGATC 1 cut(s) 385
BspQI GCTCTTC 1 cut(s) 170
BspTNI GGTCTC 1 cut(s) 346
BsrI ACTGG 1 cut(s) 276
BssMI GATC 3 cut(s) 379, 390, 519
BssSI CACGAG 1 cut(s) 386
Bst2BI CACGAG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 3 cut(s) 170, 218, 492
BstF5I GGATG 3 cut(s) 265, 300, 468
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 1 cut(s) 103
BstKTI GATC 3 cut(s) 382, 393, 522
BstMAI GTCTC 4 cut(s) 145, 159, 346, 389
BstMBI GATC 3 cut(s) 379, 390, 519
BstMCI CGRYCG 1 cut(s) 382
BstMWI GCNNNNNNNGC 1 cut(s) 76
BstNSI RCATGY 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 340
BstX2I RGATCY 1 cut(s) 519
BstYI RGATCY 1 cut(s) 519
BsuRI GGCC 2 cut(s) 149, 373
BtsCI GGATG 3 cut(s) 265, 300, 468
BtsIMutI CAGTG 1 cut(s) 546
CfoI GCGC 1 cut(s) 103
Cfr13I GGNCC 1 cut(s) 482
CviAII CATG 6 cut(s) 151, 160, 313, 357, 369, 613
CviJI RGCY 9 cut(s) 5, 79, 91, 149, 250, 279, 353, 373, 512
CviKI_1 RGCY 9 cut(s) 5, 79, 91, 149, 250, 279, 353, 373, 512
DpnI GATC 3 cut(s) 381, 392, 521
DpnII GATC 3 cut(s) 379, 390, 519
EaeI YGGCCR 2 cut(s) 147, 371
Eam1104I CTCTTC 3 cut(s) 170, 218, 492
EarI CTCTTC 3 cut(s) 170, 218, 492
Ecl136II GAGCTC 1 cut(s) 512
Eco24I GRGCYC 2 cut(s) 252, 514
Eco31I GGTCTC 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 482
Eco53kI GAGCTC 1 cut(s) 512
EcoICRI GAGCTC 1 cut(s) 512
EcoO109I RGGNCCY 1 cut(s) 482
EcoT38I GRGCYC 2 cut(s) 252, 514
Esp3I CGTCTC 1 cut(s) 389
FaeI CATG 6 cut(s) 154, 163, 316, 360, 372, 616
FaqI GGGAC 2 cut(s) 64, 98
FatI CATG 6 cut(s) 150, 159, 312, 356, 368, 612
FauNDI CATATG 1 cut(s) 55
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 1 cut(s) 354
FokI GGATG 3 cut(s) 272, 287, 455
FriOI GRGCYC 2 cut(s) 252, 514
Fsp4HI GCNGC 1 cut(s) 354
GlaI GCGC 1 cut(s) 102
GluI GCNGC 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 2 cut(s) 149, 373
HhaI GCGC 1 cut(s) 103
Hin1II CATG 6 cut(s) 154, 163, 316, 360, 372, 616
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HinfI GANTC 1 cut(s) 407
Hpy166II GTNNAC 2 cut(s) 334, 362
Hpy188I TCNGA 5 cut(s) 27, 45, 379, 598, 610
Hpy188III TCNNGA 3 cut(s) 217, 257, 388
Hpy8I GTNNAC 2 cut(s) 334, 362
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4IV ACGT 1 cut(s) 38
HpyCH4V TGCA 3 cut(s) 65, 356, 639
HpyF10VI GCNNNNNNNGC 1 cut(s) 76
HpySE526I ACGT 1 cut(s) 38
Hsp92II CATG 6 cut(s) 154, 163, 316, 360, 372, 616
HspAI GCGC 1 cut(s) 101
Kzo9I GATC 3 cut(s) 379, 390, 519
LguI GCTCTTC 1 cut(s) 170
LmnI GCTCC 5 cut(s) 88, 284, 350, 517, 578
LpnPI CCDG 7 cut(s) 45, 51, 202, 240, 242, 257, 306
Lsp1109I GCAGC 1 cut(s) 340
MaeII ACGT 1 cut(s) 38
MalI GATC 3 cut(s) 381, 392, 521
MboI GATC 3 cut(s) 379, 390, 519
MboII GAAGA 3 cut(s) 157, 205, 479
MflI RGATCY 1 cut(s) 519
MhlI GDGCHC 2 cut(s) 252, 514
MlsI TGGCCA 1 cut(s) 149
MluCI AATT 8 cut(s) 21, 59, 72, 283, 456, 564, 603, 626
MluNI TGGCCA 1 cut(s) 149
MmeI TCCRAC 1 cut(s) 512
MnlI CCTC 7 cut(s) 41, 126, 168, 300, 322, 453, 495
Mox20I TGGCCA 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 39
MscI TGGCCA 1 cut(s) 149
MseI TTAA 2 cut(s) 459, 651
Msp20I TGGCCA 1 cut(s) 149
MwoI GCNNNNNNNGC 1 cut(s) 76
NdeI CATATG 1 cut(s) 55
NdeII GATC 3 cut(s) 379, 390, 519
NlaIII CATG 6 cut(s) 154, 163, 316, 360, 372, 616
NlaIV GGNNCC 1 cut(s) 483
NspI RCATGY 1 cut(s) 360
PciSI GCTCTTC 1 cut(s) 170
PdmI GAANNNNTTC 1 cut(s) 39
PfeI GAWTC 1 cut(s) 407
PkrI GCNGC 1 cut(s) 355
Ple19I CGATCG 1 cut(s) 382
PpuMI RGGWCCY 1 cut(s) 482
Psp124BI GAGCTC 1 cut(s) 514
Psp1406I AACGTT 1 cut(s) 38
Psp5II RGGWCCY 1 cut(s) 482
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 1 cut(s) 482
PspPPI RGGWCCY 1 cut(s) 482
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PsuI RGATCY 1 cut(s) 519
PvuI CGATCG 1 cut(s) 382
SacI GAGCTC 1 cut(s) 514
SapI GCTCTTC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 459, 651
SatI GCNGC 1 cut(s) 354
Sau3AI GATC 3 cut(s) 379, 390, 519
Sau96I GGNCC 1 cut(s) 482
SduI GDGCHC 2 cut(s) 252, 514
SetI ASST 9 cut(s) 41, 93, 267, 281, 333, 342, 355, 432, 514
SinI GGWCC 1 cut(s) 482
Sse9I AATT 8 cut(s) 21, 59, 72, 283, 456, 564, 603, 626
SstI GAGCTC 1 cut(s) 514
TaaI ACNGT 1 cut(s) 127
TaiI ACGT 1 cut(s) 41
TasI AATT 8 cut(s) 21, 59, 72, 283, 456, 564, 603, 626
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 2 cut(s) 459, 651
Tru9I TTAA 2 cut(s) 459, 651
TscAI CASTG 1 cut(s) 553
TseI GCWGC 1 cut(s) 353
TspDTI ATGAA 3 cut(s) 72, 336, 357
TspRI CASTG 1 cut(s) 553
VpaK11BI GGWCC 1 cut(s) 482
XapI RAATTY 2 cut(s) 21, 59
XceI RCATGY 1 cut(s) 360
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.