pycom17g15010

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0021197
Physical Location & Seq
Reverse (-)
12765989 .. 12766411
423 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 423 bp
ATGTCCGAGAAGGGTTTGCTCCATATCTTTGTTTTGTTAACTTTTTGGGTGTTAATCTTCCATGCTCGGGTAGGAGCTTGCAAGGGGAAGGCCACTCATCCTGGTTCTACTTTCGCTCGAACACAAGGAACCCGTTTTGTTATGAATGGAAAGCCGTTTTACATAAACGGGTTCAATGCATATTGGATGATGTACATGGCATCGGACCCATCTACAAGGGCCAAGGTCACTTCTGCACTCCAACAAGCCTCCAAGTATGGCATGAATGTTGCTAGGGTTTGGGCTTTCAGTGACGGCGATAACGATAGACCCCTTCAGCCTTCTCCTGGTTCATACAACGAGGACACGTTCAAGGTACGTACTACAGTACTTCATACCGTACGTATGTCTTTATGTATGTATAATCTAAACTACTACAACTAA

Protein Analysis

141

Amino Acids

15.91

Weight (kDa)

9.73

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH5_mannosidase PF26410 37 - 126 1.6e-09 GH family 5 beta-mannosidase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021197)

Species Orthologous Gene IDs
malus_domestica MD17G1156600.v1.1
pyrus_communis pycom09g08800 pycom17g15010
rosa_multiflora Rmu_sc0000435.1_g000044
rosa_samantha Rh5BG462600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 299
AfaI GTAC 5 cut(s) 194, 357, 361, 369, 381
AfiI CCNNNNNNNGG 2 cut(s) 67, 326
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 2 cut(s) 175, 352
AjnI CCWGG 2 cut(s) 100, 325
AluBI AGCT 1 cut(s) 77
AluI AGCT 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 66
AoxI GGCC 2 cut(s) 90, 219
AspS9I GGNCC 2 cut(s) 205, 219
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 1 cut(s) 205
BccI CCATC 1 cut(s) 217
BceAI ACGGC 2 cut(s) 139, 310
BciT130I CCWGG 2 cut(s) 102, 327
BfaI CTAG 1 cut(s) 273
BfmI CTRYAG 1 cut(s) 363
BmcAI AGTACT 1 cut(s) 369
Bme1390I CCNGG 2 cut(s) 102, 327
Bme18I GGWCC 1 cut(s) 205
BmeT110I CYCGRG 1 cut(s) 66
BmgT120I GGNCC 2 cut(s) 205, 219
BmiI GGNNCC 2 cut(s) 130, 207
BmrFI CCNGG 2 cut(s) 102, 327
BmsI GCATC 1 cut(s) 209
BsaAI YACGTR 2 cut(s) 359, 383
BsaJI CCNNGG 1 cut(s) 222
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 326
BseBI CCWGG 2 cut(s) 102, 327
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 2 cut(s) 97, 192
BseLI CCNNNNNNNGG 2 cut(s) 67, 326
BsgI GTGCAG 1 cut(s) 219
BshFI GGCC 2 cut(s) 92, 221
BsiHKCI CYCGRG 1 cut(s) 66
BsiWI CGTACG 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 67, 326
BsnI GGCC 2 cut(s) 92, 221
BsoBI CYCGRG 1 cut(s) 66
Bsp1407I TGTACA 1 cut(s) 192
BspANI GGCC 2 cut(s) 92, 221
BspLI GGNNCC 2 cut(s) 130, 207
BsrGI TGTACA 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 2 cut(s) 102, 327
Bst4CI ACNGT 2 cut(s) 367, 379
BstAUI TGTACA 1 cut(s) 192
BstBAI YACGTR 2 cut(s) 359, 383
BstC8I GCNNGC 1 cut(s) 79
BstF5I GGATG 2 cut(s) 97, 192
BstNI CCWGG 2 cut(s) 102, 327
BstSCI CCNGG 2 cut(s) 100, 325
BstSFI CTRYAG 1 cut(s) 363
BstSNI TACGTA 2 cut(s) 359, 383
BsuRI GGCC 2 cut(s) 92, 221
BtsCI GGATG 2 cut(s) 97, 192
BtsIMutI CAGTG 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 205, 219
Csp6I GTAC 5 cut(s) 193, 356, 360, 368, 380
CviAII CATG 3 cut(s) 62, 196, 262
CviJI RGCY 7 cut(s) 77, 92, 154, 221, 248, 284, 319
CviKI_1 RGCY 7 cut(s) 77, 92, 154, 221, 248, 284, 319
CviQI GTAC 5 cut(s) 193, 356, 360, 368, 380
Eco105I TACGTA 2 cut(s) 359, 383
Eco130I CCWWGG 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 66
EcoRII CCWGG 2 cut(s) 100, 325
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 3 cut(s) 65, 199, 265
FatI CATG 3 cut(s) 61, 195, 261
FokI GGATG 2 cut(s) 84, 199
FspBI CTAG 1 cut(s) 273
HaeIII GGCC 2 cut(s) 92, 221
Hin1II CATG 3 cut(s) 65, 199, 265
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HpaI GTTAAC 1 cut(s) 39
Hpy166II GTNNAC 1 cut(s) 39
Hpy188I TCNGA 2 cut(s) 7, 205
Hpy8I GTNNAC 1 cut(s) 39
HpyAV CCTTC 4 cut(s) 4, 82, 323, 330
HpyCH4III ACNGT 2 cut(s) 367, 379
HpyCH4IV ACGT 3 cut(s) 347, 358, 382
HpyCH4V TGCA 3 cut(s) 81, 179, 236
HpySE526I ACGT 3 cut(s) 347, 358, 382
Hsp92II CATG 3 cut(s) 65, 199, 265
KspAI GTTAAC 1 cut(s) 39
LmnI GCTCC 2 cut(s) 24, 74
LpnPI CCDG 4 cut(s) 87, 114, 312, 339
LweI GCATC 1 cut(s) 209
MaeI CTAG 1 cut(s) 273
MaeII ACGT 3 cut(s) 347, 358, 382
MaeIII GTNAC 2 cut(s) 226, 290
MboII GAAGA 1 cut(s) 49
MmeI TCCRAC 1 cut(s) 265
MnlI CCTC 2 cut(s) 259, 334
Mph1103I ATGCAT 1 cut(s) 181
MseI TTAA 2 cut(s) 38, 53
MspR9I CCNGG 2 cut(s) 102, 327
MvaI CCWGG 2 cut(s) 102, 327
NlaIII CATG 3 cut(s) 65, 199, 265
NlaIV GGNNCC 2 cut(s) 130, 207
NmuCI GTSAC 2 cut(s) 226, 290
NsiI ATGCAT 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 300
Pfl23II CGTACG 1 cut(s) 379
Ppu21I YACGTR 2 cut(s) 359, 383
Psp6I CCWGG 2 cut(s) 100, 325
PspGI CCWGG 2 cut(s) 100, 325
PspLI CGTACG 1 cut(s) 379
PspN4I GGNNCC 2 cut(s) 130, 207
PspPI GGNCC 2 cut(s) 205, 219
RsaI GTAC 5 cut(s) 194, 357, 361, 369, 381
RsaNI GTAC 5 cut(s) 193, 356, 360, 368, 380
SaqAI TTAA 2 cut(s) 38, 53
Sau96I GGNCC 2 cut(s) 205, 219
ScaI AGTACT 1 cut(s) 369
ScrFI CCNGG 2 cut(s) 102, 327
SetI ASST 6 cut(s) 79, 228, 350, 357, 361, 385
SfaNI GCATC 1 cut(s) 209
SfcI CTRYAG 1 cut(s) 363
SinI GGWCC 1 cut(s) 205
SnaBI TACGTA 2 cut(s) 359, 383
SspMI CTAG 1 cut(s) 273
StyD4I CCNGG 2 cut(s) 100, 325
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 2 cut(s) 367, 379
TaiI ACGT 3 cut(s) 350, 361, 385
TaqI TCGA 1 cut(s) 118
TatI WGTACW 2 cut(s) 192, 367
Tru1I TTAA 2 cut(s) 38, 53
Tru9I TTAA 2 cut(s) 38, 53
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 2 cut(s) 226, 290
Tsp45I GTSAC 2 cut(s) 226, 290
TspDTI ATGAA 4 cut(s) 158, 278, 321, 362
TspRI CASTG 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 205
XspI CTAG 1 cut(s) 273
ZrmI AGTACT 1 cut(s) 369
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.