pycom17g27250

Anaphase-promoting complex subunit 11 RING-H2 finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0028576
Physical Location & Seq
Forward (+)
25534864 .. 25536148
1285 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g27250.3

Sequence Viewer

Length: 366 bp
ATGAAGGACAGTGCATCTTTCCAAAGACTCGATGGGCGACTACCTTCATTACCTATTCAAGGGGCCACTTTGGCATCATCTGGAGTAGGCACAACGCTACAAAATAATCCCTTGAATGATACTCAGCTCTCAGTTTCCAAACCAACCCCCTTCGATGCTGATCAGAGATACTCGCGTTTGCAGCATGATGGCTTGGTCTCTAGGCGTGAGAAGTCAATGACTCATTTGCAAGAAGATAAAGCAACAATTGAGAAGATGCAGCTCTGGCGCCGAATCCCTGGGTTTTGGCAAATGCAACAATTATATGGTGAAAGTTTTTACACAACCTCATTACCTGCAAAATTTTGTTCAGTCAACATTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

122

Amino Acids

13.72

Weight (kDa)

9.25

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028576)

Species Orthologous Gene IDs
malus_domestica MD02G1312300.v1.1
pyrus_communis pycom17g27250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 343
AccB1I GGYRCC 1 cut(s) 267
AccII CGCG 1 cut(s) 175
AcsI RAATTY 1 cut(s) 341
AcyI GRCGYC 1 cut(s) 268
AfiI CCNNNNNNNGG 1 cut(s) 59
AgsI TTSAA 3 cut(s) 59, 115, 362
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 2 cut(s) 127, 262
AluI AGCT 2 cut(s) 127, 262
Alw26I GTCTC 1 cut(s) 202
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 2 cut(s) 181, 259
ApoI RAATTY 1 cut(s) 341
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 1 cut(s) 320
BaeI ACNNNNGTAYC 2 cut(s) 160, 193
BanI GGYRCC 1 cut(s) 267
BbvI GCAGC 2 cut(s) 193, 271
BccI CCATC 2 cut(s) 26, 182
BciT130I CCWGG 1 cut(s) 279
BclI TGATCA 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 202
BfaI CTAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 271
BfuAI ACCTGC 1 cut(s) 343
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 2 cut(s) 182, 260
BlsI GCNGC 2 cut(s) 183, 261
Bme1390I CCNGG 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 2 cut(s) 64, 269
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 4 cut(s) 23, 83, 145, 246
BpmI CTGGAG 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 277, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 59
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 277, 278
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 59
BseMII CTCAG 2 cut(s) 137, 144
BseXI GCAGC 2 cut(s) 193, 271
Bsh1236I CGCG 1 cut(s) 175
BshFI GGCC 1 cut(s) 65
BshNI GGYRCC 1 cut(s) 267
BslI CCNNNNNNNGG 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 202
BsnI GGCC 1 cut(s) 65
Bso31I GGTCTC 1 cut(s) 202
Bsp143I GATC 1 cut(s) 160
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 2 cut(s) 136, 143
BspFNI CGCG 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 64, 269
BspMI ACCTGC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 267
BspTNI GGTCTC 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 277, 278
BssMI GATC 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 268
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 268
BstDEI CTNAG 2 cut(s) 123, 130
BstENI CCTNNNNNAGG 1 cut(s) 57
BstFNI CGCG 1 cut(s) 175
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 1 cut(s) 163
BstMAI GTCTC 1 cut(s) 202
BstMBI GATC 1 cut(s) 160
BstMWI GCNNNNNNNGC 3 cut(s) 71, 181, 265
BstNI CCWGG 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 277
BstUI CGCG 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 193, 271
BsuRI GGCC 1 cut(s) 65
BtsIMutI CAGTG 1 cut(s) 16
BveI ACCTGC 1 cut(s) 343
CfoI GCGC 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 63
CviAII CATG 1 cut(s) 185
CviJI RGCY 4 cut(s) 65, 127, 192, 262
CviKI_1 RGCY 4 cut(s) 65, 127, 192, 262
DdeI CTNAG 2 cut(s) 123, 130
DinI GGCGCC 1 cut(s) 269
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 202
EcoNI CCTNNNNNAGG 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 277
EgeI GGCGCC 1 cut(s) 269
EheI GGCGCC 1 cut(s) 269
FaeI CATG 1 cut(s) 188
FaiI YATR 3 cut(s) 186, 304, 306
FatI CATG 1 cut(s) 184
FbaI TGATCA 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 182, 260
Fsp4HI GCNGC 2 cut(s) 182, 260
FspBI CTAG 1 cut(s) 201
GlaI GCGC 1 cut(s) 269
GluI GCNGC 2 cut(s) 182, 260
GsuI CTGGAG 1 cut(s) 102
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 1 cut(s) 65
HhaI GCGC 1 cut(s) 270
Hin1I GRCGYC 1 cut(s) 268
Hin1II CATG 1 cut(s) 188
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 3 cut(s) 27, 220, 273
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 1 cut(s) 165
Hpy188III TCNNGA 1 cut(s) 81
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 54, 160
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4V TGCA 6 cut(s) 14, 181, 229, 259, 295, 338
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 181, 265
HpyF3I CTNAG 2 cut(s) 123, 130
Hsp92I GRCGYC 1 cut(s) 268
Hsp92II CATG 1 cut(s) 188
HspAI GCGC 1 cut(s) 268
KasI GGCGCC 1 cut(s) 267
Ksp22I TGATCA 1 cut(s) 160
Kzo9I GATC 1 cut(s) 160
LpnPI CCDG 5 cut(s) 66, 250, 264, 291, 348
Lsp1109I GCAGC 2 cut(s) 193, 271
LweI GCATC 4 cut(s) 23, 83, 145, 246
MaeI CTAG 1 cut(s) 201
MalI GATC 1 cut(s) 162
MboI GATC 1 cut(s) 160
MboII GAAGA 2 cut(s) 245, 265
MfeI CAATTG 1 cut(s) 246
MluCI AATT 3 cut(s) 246, 299, 341
Mly113I GGCGCC 1 cut(s) 268
MlyI GAGTC 2 cut(s) 21, 214
MnlI CCTC 1 cut(s) 337
MspR9I CCNGG 1 cut(s) 279
MunI CAATTG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 279
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 3 cut(s) 71, 181, 265
NarI GGCGCC 1 cut(s) 268
NdeII GATC 1 cut(s) 160
NlaIII CATG 1 cut(s) 188
NlaIV GGNNCC 2 cut(s) 64, 269
PasI CCCWGGG 1 cut(s) 278
PfeI GAWTC 1 cut(s) 273
PkrI GCNGC 2 cut(s) 183, 261
PleI GAGTC 2 cut(s) 21, 214
PluTI GGCGCC 1 cut(s) 271
PpsI GAGTC 2 cut(s) 21, 214
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 64, 269
PspPI GGNCC 1 cut(s) 63
SatI GCNGC 2 cut(s) 182, 260
Sau3AI GATC 1 cut(s) 160
Sau96I GGNCC 1 cut(s) 63
SchI GAGTC 2 cut(s) 21, 214
ScrFI CCNGG 1 cut(s) 279
SetI ASST 6 cut(s) 46, 55, 129, 264, 329, 337
SfaNI GCATC 4 cut(s) 23, 83, 145, 246
SfoI GGCGCC 1 cut(s) 269
Sse9I AATT 3 cut(s) 246, 299, 341
SspDI GGCGCC 1 cut(s) 267
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 277
TaaI ACNGT 1 cut(s) 11
TaqI TCGA 2 cut(s) 30, 153
TasI AATT 3 cut(s) 246, 299, 341
TfiI GAWTC 1 cut(s) 273
TscAI CASTG 1 cut(s) 16
TseI GCWGC 2 cut(s) 181, 259
TspDTI ATGAA 2 cut(s) 17, 36
TspRI CASTG 1 cut(s) 16
XagI CCTNNNNNAGG 1 cut(s) 57
XapI RAATTY 1 cut(s) 341
XcmI CCANNNNNNNNNTGG 1 cut(s) 29
XspI CTAG 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.