pycom2575g00030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002575
OG0018538
Physical Location & Seq
Forward (+)
12895 .. 13152
258 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2575g00030.1

Sequence Viewer

Length: 258 bp
ATGTTCGCTGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCTTTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCCAGGCTGGGACCCCCGTGCCCATTCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

86

Amino Acids

9.26

Weight (kDa)

11.86

Isoelectric Point (pI)

71.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 74
AccII CGCG 2 cut(s) 64, 83
AciI CCGC 6 cut(s) 64, 72, 81, 83, 103, 250
AclWI GGATC 2 cut(s) 173, 186
AcoI YGGCCR 1 cut(s) 54
AfiI CCNNNNNNNGG 4 cut(s) 18, 29, 56, 63
AjnI CCWGG 1 cut(s) 122
AluBI AGCT 1 cut(s) 92
AluI AGCT 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 234
AlwI GGATC 2 cut(s) 173, 186
AlwNI CAGNNNCTG 1 cut(s) 227
Ama87I CYCGRG 2 cut(s) 12, 93
AoxI GGCC 2 cut(s) 20, 54
ApeKI GCWGC 2 cut(s) 8, 89
AspS9I GGNCC 3 cut(s) 21, 32, 131
AsuC2I CCSGG 3 cut(s) 13, 14, 53
AsuHPI GGTGA 1 cut(s) 224
AvaI CYCGRG 2 cut(s) 12, 93
AvaII GGWCC 2 cut(s) 32, 131
BaeGI GKGCMC 1 cut(s) 143
BamHI GGATCC 1 cut(s) 178
BbvI GCAGC 1 cut(s) 101
BccI CCATC 1 cut(s) 222
BciT130I CCWGG 1 cut(s) 124
BcnI CCSGG 3 cut(s) 13, 14, 53
BcoDI GTCTC 1 cut(s) 234
BisI GCNGC 4 cut(s) 9, 65, 81, 90
BlsI GCNGC 4 cut(s) 10, 66, 82, 91
Bme1390I CCNGG 4 cut(s) 13, 14, 53, 124
Bme18I GGWCC 2 cut(s) 32, 131
BmeT110I CYCGRG 2 cut(s) 12, 93
BmgT120I GGNCC 3 cut(s) 21, 32, 131
BmiI GGNNCC 6 cut(s) 23, 33, 121, 132, 133, 180
BmrFI CCNGG 4 cut(s) 13, 14, 53, 124
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 1 cut(s) 237
BmuI ACTGGG 1 cut(s) 35
BpuMI CCSGG 3 cut(s) 13, 14, 53
BsaI GGTCTC 1 cut(s) 234
BsaJI CCNNGG 5 cut(s) 12, 13, 51, 81, 235
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 29, 56, 63
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 41
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 5 cut(s) 12, 13, 51, 81, 235
BseLI CCNNNNNNNGG 4 cut(s) 18, 29, 56, 63
BseMII CTCAG 1 cut(s) 163
BseNI ACTGG 1 cut(s) 41
BseSI GKGCMC 1 cut(s) 143
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 1 cut(s) 101
BseYI CCCAGC 1 cut(s) 127
Bsh1236I CGCG 2 cut(s) 64, 83
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 2 cut(s) 22, 56
BsiEI CGRYCG 1 cut(s) 57
BsiHKCI CYCGRG 2 cut(s) 12, 93
BsiSI CCGG 3 cut(s) 13, 53, 57
BslFI GGGAC 2 cut(s) 45, 144
BslI CCNNNNNNNGG 4 cut(s) 18, 29, 56, 63
BsmAI GTCTC 1 cut(s) 234
BsmFI GGGAC 2 cut(s) 45, 144
BsnI GGCC 2 cut(s) 22, 56
Bso31I GGTCTC 1 cut(s) 234
BsoBI CYCGRG 2 cut(s) 12, 93
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 1 cut(s) 178
BspACI CCGC 6 cut(s) 64, 72, 81, 83, 103, 250
BspANI GGCC 2 cut(s) 22, 56
BspCNI CTCAG 1 cut(s) 162
BspFNI CGCG 2 cut(s) 64, 83
BspLI GGNNCC 6 cut(s) 23, 33, 121, 132, 133, 180
BspPI GGATC 2 cut(s) 173, 186
BspTNI GGTCTC 1 cut(s) 234
BsrBI CCGCTC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 41
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 5 cut(s) 12, 13, 51, 81, 235
BssMI GATC 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 235
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 1 cut(s) 36
BstC8I GCNNGC 2 cut(s) 58, 62
BstDEI CTNAG 1 cut(s) 149
BstDSI CCRYGG 1 cut(s) 81
BstFNI CGCG 2 cut(s) 64, 83
BstKTI GATC 1 cut(s) 181
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 1 cut(s) 178
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 4 cut(s) 80, 86, 89, 95
BstNI CCWGG 1 cut(s) 124
BstSCI CCNGG 4 cut(s) 11, 12, 51, 122
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 2 cut(s) 64, 83
BstV1I GCAGC 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BstZI CGGCCG 1 cut(s) 54
BsuRI GGCC 2 cut(s) 22, 56
BtgI CCRYGG 1 cut(s) 81
Cac8I GCNNGC 2 cut(s) 58, 62
CaiI CAGNNNCTG 1 cut(s) 227
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 3 cut(s) 21, 32, 131
Cfr42I CCGCGG 1 cut(s) 84
Cfr9I CCCGGG 1 cut(s) 12
CviJI RGCY 7 cut(s) 22, 56, 92, 112, 127, 155, 227
CviKI_1 RGCY 7 cut(s) 22, 56, 92, 112, 127, 155, 227
DdeI CTNAG 1 cut(s) 149
DpnI GATC 1 cut(s) 180
DpnII GATC 1 cut(s) 178
EaeI YGGCCR 1 cut(s) 54
EagI CGGCCG 1 cut(s) 54
EciI GGCGGA 1 cut(s) 92
EclXI CGGCCG 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 235
Eco31I GGTCTC 1 cut(s) 234
Eco47I GGWCC 2 cut(s) 32, 131
Eco52I CGGCCG 1 cut(s) 54
Eco88I CYCGRG 2 cut(s) 12, 93
EcoO109I RGGNCCY 2 cut(s) 21, 131
EcoRII CCWGG 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 235
FaiI YATR 1 cut(s) 256
FaqI GGGAC 2 cut(s) 45, 144
FauI CCCGC 2 cut(s) 76, 79
Fnu4HI GCNGC 4 cut(s) 9, 65, 81, 90
Fsp4HI GCNGC 4 cut(s) 9, 65, 81, 90
GluI GCNGC 4 cut(s) 9, 65, 81, 90
GsaI CCCAGC 1 cut(s) 131
HaeIII GGCC 2 cut(s) 22, 56
HapII CCGG 3 cut(s) 13, 53, 57
HpaII CCGG 3 cut(s) 13, 53, 57
HphI GGTGA 1 cut(s) 224
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 1 cut(s) 152
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 1 cut(s) 118
HpyAV CCTTC 2 cut(s) 12, 22
HpyCH4III ACNGT 1 cut(s) 36
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 86, 89, 95
HpyF3I CTNAG 1 cut(s) 149
KflI GGGWCCC 1 cut(s) 131
KroI GCCGGC 1 cut(s) 56
KroNI GCCGGC 1 cut(s) 58
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 1 cut(s) 178
LmnI GCTCC 1 cut(s) 86
LpnPI CCDG 8 cut(s) 26, 54, 66, 70, 109, 113, 136, 234
Lsp1109I GCAGC 1 cut(s) 101
LweI GCATC 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 172
MalI GATC 1 cut(s) 180
MbiI CCGCTC 1 cut(s) 74
MboI GATC 1 cut(s) 178
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 143
MnlI CCTC 2 cut(s) 158, 217
MroNI GCCGGC 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 3 cut(s) 13, 53, 57
MspR9I CCNGG 4 cut(s) 13, 14, 53, 124
MvaI CCWGG 1 cut(s) 124
MvnI CGCG 2 cut(s) 64, 83
MwoI GCNNNNNNNGC 4 cut(s) 80, 86, 89, 95
NaeI GCCGGC 1 cut(s) 58
NciI CCSGG 3 cut(s) 13, 14, 53
NdeII GATC 1 cut(s) 178
NgoMIV GCCGGC 1 cut(s) 56
NlaIV GGNNCC 6 cut(s) 23, 33, 121, 132, 133, 180
PaeR7I CTCGAG 1 cut(s) 93
PdiI GCCGGC 1 cut(s) 58
PkrI GCNGC 4 cut(s) 10, 66, 82, 91
PpuMI RGGWCCY 1 cut(s) 131
Psp5II RGGWCCY 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 122
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 6 cut(s) 23, 33, 121, 132, 133, 180
PspPI GGNCC 3 cut(s) 21, 32, 131
PspPPI RGGWCCY 1 cut(s) 131
PspXI VCTCGAGB 1 cut(s) 93
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 1 cut(s) 178
SacII CCGCGG 1 cut(s) 84
SatI GCNGC 4 cut(s) 9, 65, 81, 90
Sau3AI GATC 1 cut(s) 178
Sau96I GGNCC 3 cut(s) 21, 32, 131
ScrFI CCNGG 4 cut(s) 13, 14, 53, 124
SduI GDGCHC 1 cut(s) 143
SetI ASST 3 cut(s) 94, 237, 246
SfaNI GCATC 1 cut(s) 237
Sfr274I CTCGAG 1 cut(s) 93
Sfr303I CCGCGG 1 cut(s) 84
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 2 cut(s) 32, 131
SlaI CTCGAG 1 cut(s) 93
SmaI CCCGGG 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
SsiI CCGC 6 cut(s) 64, 72, 81, 83, 103, 250
StyD4I CCNGG 4 cut(s) 11, 12, 51, 122
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 2 cut(s) 94, 217
TauI GCSGC 2 cut(s) 67, 83
TseI GCWGC 2 cut(s) 8, 89
TspGWI ACGGA 1 cut(s) 191
TspMI CCCGGG 1 cut(s) 12
VpaK11BI GGWCC 2 cut(s) 32, 131
XhoI CTCGAG 1 cut(s) 93
XmaI CCCGGG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.