Rmu_sc0005685.1_g000030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005685.1
OG0018538
Physical Location & Seq
Reverse (-)
134579 .. 137337
2759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005685.1_g000030.1.cds

Sequence Viewer

Length: 591 bp
atggttagcctcgttagactggaaaggctacatggccaagatggcttgtcagtaagacactcaaggcttgagaatgcgggtagttctgagtcaactgttgaacgcccggggaaaacccccgaaggagagttcccagtccatcccccgatcggcacgcagcgactctctcttaccgcggaagcaggtcgagcagttcaccgaaagccgacgggttcgggattgggacccccgtgcctagccctcagagccaatcattttcctgagattttcaagggccgtcgggggtgcaccggacaccatatgacatgcggtgatcttccagccgctggaccctatctccggctgagccgtttccagggggctccctgcgcggtgccagctttggcaaaggaatgctacttgattgatcttgccagtagtcatatgcttgtcttaaagactaagtcatgcatgtgtgtgcaccggtcggctcatcccttctaccggcgatacgctcctagctttaattggccaggtcgtgcctccggtgctgttactttgaagaaattagagtgctcaaagcaagcttacgctctgaatacattagcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.27

Weight (kDa)

9.76

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 173
AccB1I GGYRCC 1 cut(s) 373
AccB7I CCANNNNNTGG 1 cut(s) 326
AccII CGCG 2 cut(s) 176, 371
AciI CCGC 6 cut(s) 77, 174, 176, 309, 324, 371
AcoI YGGCCR 2 cut(s) 34, 509
AfiI CCNNNNNNNGG 5 cut(s) 149, 326, 339, 355, 483
AgeI ACCGGT 1 cut(s) 462
AgsI TTSAA 3 cut(s) 101, 271, 541
AjnI CCWGG 2 cut(s) 354, 511
AluBI AGCT 3 cut(s) 380, 501, 566
AluI AGCT 3 cut(s) 380, 501, 566
Alw21I GWGCWC 3 cut(s) 290, 462, 557
Alw44I GTGCAC 2 cut(s) 286, 458
AlwNI CAGNNNCTG 1 cut(s) 326
Ama87I CYCGRG 1 cut(s) 106
AoxI GGCC 3 cut(s) 34, 274, 509
ApaLI GTGCAC 2 cut(s) 286, 458
ApeKI GCWGC 1 cut(s) 157
AsiGI ACCGGT 1 cut(s) 462
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 3 cut(s) 224, 274, 329
AsuC2I CCSGG 2 cut(s) 107, 108
AsuHPI GGTGA 2 cut(s) 188, 323
AvaI CYCGRG 1 cut(s) 106
AvaII GGWCC 2 cut(s) 224, 329
BaeGI GKGCMC 2 cut(s) 290, 462
BalI TGGCCA 2 cut(s) 36, 511
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 1 cut(s) 364
Bbv12I GWGCWC 3 cut(s) 290, 462, 557
BbvI GCAGC 1 cut(s) 169
BccI CCATC 2 cut(s) 35, 147
BceAI ACGGC 2 cut(s) 261, 333
BciT130I CCWGG 2 cut(s) 356, 513
BcnI CCSGG 2 cut(s) 107, 108
BfaI CTAG 2 cut(s) 236, 498
BfuAI ACCTGC 1 cut(s) 173
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 2 cut(s) 158, 324
BlpI GCTNAGC 1 cut(s) 344
BlsI GCNGC 2 cut(s) 159, 325
Bme1390I CCNGG 4 cut(s) 107, 108, 356, 513
Bme18I GGWCC 2 cut(s) 224, 329
BmeT110I CYCGRG 1 cut(s) 106
BmgT120I GGNCC 3 cut(s) 224, 274, 329
BmiI GGNNCC 5 cut(s) 225, 226, 331, 363, 375
BmrFI CCNGG 4 cut(s) 107, 108, 356, 513
BmrI ACTGGG 1 cut(s) 128
BmuI ACTGGG 1 cut(s) 128
Bpu1102I GCTNAGC 1 cut(s) 344
BpuEI CTTGAG 2 cut(s) 46, 89
BpuMI CCSGG 2 cut(s) 107, 108
BsaJI CCNNGG 4 cut(s) 106, 107, 174, 355
BsaWI WCCGGW 3 cut(s) 290, 462, 524
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 5 cut(s) 149, 326, 339, 355, 483
Bse118I RCCGGY 2 cut(s) 462, 483
Bse1I ACTGG 3 cut(s) 24, 134, 414
BseBI CCWGG 2 cut(s) 356, 513
BseDI CCNNGG 4 cut(s) 106, 107, 174, 355
BseGI GGATG 2 cut(s) 139, 472
BseLI CCNNNNNNNGG 5 cut(s) 149, 326, 339, 355, 483
BseMII CTCAG 4 cut(s) 78, 252, 256, 335
BseNI ACTGG 3 cut(s) 24, 134, 414
BseSI GKGCMC 2 cut(s) 290, 462
BseXI GCAGC 1 cut(s) 169
Bsh1236I CGCG 2 cut(s) 176, 371
Bsh1285I CGRYCG 2 cut(s) 150, 467
BshFI GGCC 3 cut(s) 36, 276, 511
BshNI GGYRCC 1 cut(s) 373
BshTI ACCGGT 1 cut(s) 462
BsiEI CGRYCG 2 cut(s) 150, 467
BsiHKAI GWGCWC 3 cut(s) 290, 462, 557
BsiHKCI CYCGRG 1 cut(s) 106
BsiSI CCGG 6 cut(s) 107, 291, 340, 463, 484, 525
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 5 cut(s) 149, 326, 339, 355, 483
BsmFI GGGAC 1 cut(s) 237
BsmI GAATGC 2 cut(s) 79, 398
BsnI GGCC 3 cut(s) 36, 276, 511
BsoBI CYCGRG 1 cut(s) 106
Bsp1286I GDGCHC 4 cut(s) 290, 364, 462, 557
Bsp143I GATC 3 cut(s) 147, 313, 406
Bsp1720I GCTNAGC 1 cut(s) 344
BspACI CCGC 6 cut(s) 77, 174, 176, 309, 324, 371
BspANI GGCC 3 cut(s) 36, 276, 511
BspCNI CTCAG 4 cut(s) 79, 253, 255, 336
BspFNI CGCG 2 cut(s) 176, 371
BspLI GGNNCC 5 cut(s) 225, 226, 331, 363, 375
BspMI ACCTGC 1 cut(s) 173
BspT107I GGYRCC 1 cut(s) 373
BsrFI RCCGGY 2 cut(s) 462, 483
BsrI ACTGG 3 cut(s) 24, 134, 414
BssAI RCCGGY 2 cut(s) 462, 483
BssECI CCNNGG 4 cut(s) 106, 107, 174, 355
BssMI GATC 3 cut(s) 147, 313, 406
Bst2UI CCWGG 2 cut(s) 356, 513
Bst4CI ACNGT 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 155, 378, 564
BstDEI CTNAG 5 cut(s) 87, 242, 261, 344, 441
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 2 cut(s) 139, 472
BstFNI CGCG 2 cut(s) 176, 371
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 3 cut(s) 150, 316, 409
BstMBI GATC 3 cut(s) 147, 313, 406
BstMCI CGRYCG 2 cut(s) 150, 467
BstMWI GCNNNNNNNGC 6 cut(s) 42, 188, 245, 368, 377, 527
BstNI CCWGG 2 cut(s) 356, 513
BstNSI RCATGY 2 cut(s) 309, 454
BstSCI CCNGG 4 cut(s) 105, 106, 354, 511
BstSLI GKGCMC 2 cut(s) 290, 462
BstUI CGCG 2 cut(s) 176, 371
BstV1I GCAGC 1 cut(s) 169
BsuRI GGCC 3 cut(s) 36, 276, 511
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 2 cut(s) 139, 472
BveI ACCTGC 1 cut(s) 173
Cac8I GCNNGC 3 cut(s) 155, 378, 564
CaiI CAGNNNCTG 1 cut(s) 326
CfoI GCGC 1 cut(s) 371
Cfr10I RCCGGY 2 cut(s) 462, 483
Cfr13I GGNCC 3 cut(s) 224, 274, 329
Cfr42I CCGCGG 1 cut(s) 177
Cfr9I CCCGGG 1 cut(s) 106
CspAI ACCGGT 1 cut(s) 462
CviAII CATG 5 cut(s) 32, 306, 447, 451, 588
DdeI CTNAG 5 cut(s) 87, 242, 261, 344, 441
DpnI GATC 3 cut(s) 149, 315, 408
DpnII GATC 3 cut(s) 147, 313, 406
EaeI YGGCCR 2 cut(s) 34, 509
Eco24I GRGCYC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 224, 329
Eco88I CYCGRG 1 cut(s) 106
EcoO109I RGGNCCY 1 cut(s) 224
EcoRII CCWGG 2 cut(s) 354, 511
EcoT22I ATGCAT 1 cut(s) 452
EcoT38I GRGCYC 1 cut(s) 364
FaeI CATG 5 cut(s) 35, 309, 450, 454, 591
FaiI YATR 9 cut(s) 33, 300, 302, 307, 423, 425, 448, 452, 589
FaqI GGGAC 1 cut(s) 237
FatI CATG 5 cut(s) 31, 305, 446, 450, 587
FauI CCCGC 1 cut(s) 70
FauNDI CATATG 2 cut(s) 300, 423
Fnu4HI GCNGC 2 cut(s) 158, 324
FokI GGATG 2 cut(s) 126, 459
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 2 cut(s) 158, 324
FspBI CTAG 2 cut(s) 236, 498
GlaI GCGC 1 cut(s) 370
GluI GCNGC 2 cut(s) 158, 324
HaeIII GGCC 3 cut(s) 36, 276, 511
HapII CCGG 6 cut(s) 107, 291, 340, 463, 484, 525
HhaI GCGC 1 cut(s) 371
Hin1II CATG 5 cut(s) 35, 309, 450, 454, 591
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HindIII AAGCTT 1 cut(s) 564
HinfI GANTC 2 cut(s) 89, 162
HpaII CCGG 6 cut(s) 107, 291, 340, 463, 484, 525
HphI GGTGA 2 cut(s) 188, 323
Hpy166II GTNNAC 4 cut(s) 93, 196, 288, 460
Hpy188I TCNGA 3 cut(s) 88, 245, 576
Hpy188III TCNNGA 2 cut(s) 216, 260
Hpy8I GTNNAC 4 cut(s) 93, 196, 288, 460
Hpy99I CGWCG 2 cut(s) 211, 282
HpyAV CCTTC 2 cut(s) 116, 487
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 3 cut(s) 288, 450, 460
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 188, 245, 368, 377, 527
HpyF3I CTNAG 5 cut(s) 87, 242, 261, 344, 441
Hsp92II CATG 5 cut(s) 35, 309, 450, 454, 591
HspAI GCGC 1 cut(s) 369
KflI GGGWCCC 1 cut(s) 224
KspI CCGCGG 1 cut(s) 177
Kzo9I GATC 3 cut(s) 147, 313, 406
LmnI GCTCC 2 cut(s) 367, 499
Lsp1109I GCAGC 1 cut(s) 169
MaeI CTAG 2 cut(s) 236, 498
MaeIII GTNAC 1 cut(s) 532
MalI GATC 3 cut(s) 149, 315, 408
MboI GATC 3 cut(s) 147, 313, 406
MboII GAAGA 2 cut(s) 308, 553
MhlI GDGCHC 4 cut(s) 290, 364, 462, 557
MlsI TGGCCA 2 cut(s) 36, 511
MluCI AATT 2 cut(s) 505, 545
MluNI TGGCCA 2 cut(s) 36, 511
MlyI GAGTC 2 cut(s) 98, 156
MnlI CCTC 3 cut(s) 20, 251, 532
Mox20I TGGCCA 2 cut(s) 36, 511
Mph1103I ATGCAT 1 cut(s) 452
MscI TGGCCA 2 cut(s) 36, 511
MseI TTAA 2 cut(s) 434, 504
MslI CAYNNNNRTG 3 cut(s) 451, 455, 586
Msp20I TGGCCA 2 cut(s) 36, 511
MspA1I CMGCKG 2 cut(s) 176, 326
MspI CCGG 6 cut(s) 107, 291, 340, 463, 484, 525
MspR9I CCNGG 4 cut(s) 107, 108, 356, 513
Mva1269I GAATGC 2 cut(s) 79, 398
MvaI CCWGG 2 cut(s) 356, 513
MvnI CGCG 2 cut(s) 176, 371
MwoI GCNNNNNNNGC 6 cut(s) 42, 188, 245, 368, 377, 527
NciI CCSGG 2 cut(s) 107, 108
NdeI CATATG 2 cut(s) 300, 423
NdeII GATC 3 cut(s) 147, 313, 406
NlaIII CATG 5 cut(s) 35, 309, 450, 454, 591
NlaIV GGNNCC 5 cut(s) 225, 226, 331, 363, 375
NsiI ATGCAT 1 cut(s) 452
NspI RCATGY 2 cut(s) 309, 454
PctI GAATGC 2 cut(s) 79, 398
PflFI GACNNNGTC 1 cut(s) 442
PflMI CCANNNNNTGG 1 cut(s) 326
PinAI ACCGGT 1 cut(s) 462
PkrI GCNGC 2 cut(s) 159, 325
Ple19I CGATCG 1 cut(s) 150
PleI GAGTC 2 cut(s) 97, 156
PpsI GAGTC 2 cut(s) 97, 156
PpuMI RGGWCCY 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 354, 511
PspGI CCWGG 2 cut(s) 354, 511
PspN4I GGNNCC 5 cut(s) 225, 226, 331, 363, 375
PspPI GGNCC 3 cut(s) 224, 274, 329
PspPPI RGGWCCY 1 cut(s) 224
PstNI CAGNNNCTG 1 cut(s) 326
PsyI GACNNNGTC 1 cut(s) 442
PvuI CGATCG 1 cut(s) 150
RseI CAYNNNNRTG 3 cut(s) 451, 455, 586
SacII CCGCGG 1 cut(s) 177
SaqAI TTAA 2 cut(s) 434, 504
SatI GCNGC 2 cut(s) 158, 324
Sau3AI GATC 3 cut(s) 147, 313, 406
Sau96I GGNCC 3 cut(s) 224, 274, 329
SchI GAGTC 2 cut(s) 98, 156
ScrFI CCNGG 4 cut(s) 107, 108, 356, 513
SduI GDGCHC 4 cut(s) 290, 364, 462, 557
SetI ASST 5 cut(s) 187, 382, 503, 517, 568
Sfr303I CCGCGG 1 cut(s) 177
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 2 cut(s) 224, 329
SmaI CCCGGG 1 cut(s) 108
SmiMI CAYNNNNRTG 3 cut(s) 451, 455, 586
SmlI CTYRAG 2 cut(s) 61, 68
SmoI CTYRAG 2 cut(s) 61, 68
Sse9I AATT 2 cut(s) 505, 545
SsiI CCGC 6 cut(s) 77, 174, 176, 309, 324, 371
SspMI CTAG 2 cut(s) 236, 498
StyD4I CCNGG 4 cut(s) 105, 106, 354, 511
TaaI ACNGT 1 cut(s) 97
TaqI TCGA 1 cut(s) 187
TasI AATT 2 cut(s) 505, 545
TauI GCSGC 1 cut(s) 326
Tru1I TTAA 2 cut(s) 434, 504
Tru9I TTAA 2 cut(s) 434, 504
TseI GCWGC 1 cut(s) 157
TspMI CCCGGG 1 cut(s) 106
Tth111I GACNNNGTC 1 cut(s) 442
Van91I CCANNNNNTGG 1 cut(s) 326
VneI GTGCAC 2 cut(s) 286, 458
VpaK11BI GGWCC 2 cut(s) 224, 329
XceI RCATGY 2 cut(s) 309, 454
XmaI CCCGGG 1 cut(s) 106
XspI CTAG 2 cut(s) 236, 498
Zsp2I ATGCAT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.