pycom2749g00010

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002749
OG0024709
Physical Location & Seq
Forward (+)
2641 .. 3021
381 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 381 bp
ATGGCCAACATAGACATAGAAGGAATACTCAAGGAGCTCCCCAACGACGGCCGCATCCCCAAGACCAAGATCGTCTGCACCCTCGGCCCCTCTTCCCGCTCGGTCCCCATGGTCGAGAAGCTTCTCAGGGCTGGCATGAACGTCGCCCGCTTCAACTTCTCTCATGGCACCCACGACTACCACCAGGAGACCCTCGACAATCTCCGCACTGCCATGCACAACACTCAGATCCTCTGCGCCGTGATGCTCGATACAAAGGTCCCTATATATCCATCTTTTTCCTCATTAATTTCTCAATTTCTCTTTCCTATACATACATACTTCTGCAGCTACACAAAAACATATACACACACACACACACACCCTCACTTTCCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

127

Amino Acids

14.36

Weight (kDa)

8.5

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 20 - 88 2.3e-26 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024709)

Species Orthologous Gene IDs
pyrus_communis pycom2749g00010
rosa_chinensis RchiOBHm_Chr5g0044381
rosa_samantha Rh6CG468200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccBSI CCGCTC 1 cut(s) 99
AciI CCGC 4 cut(s) 52, 97, 148, 205
AclWI GGATC 1 cut(s) 223
AcoI YGGCCR 2 cut(s) 3, 49
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 1 cut(s) 154
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 3 cut(s) 37, 121, 330
AluI AGCT 3 cut(s) 37, 121, 330
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 1 cut(s) 223
AoxI GGCC 3 cut(s) 3, 49, 85
ApeKI GCWGC 1 cut(s) 327
AseI ATTAAT 1 cut(s) 287
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 3 cut(s) 86, 103, 259
AvaII GGWCC 2 cut(s) 103, 259
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 339
BccI CCATC 1 cut(s) 280
BceAI ACGGC 2 cut(s) 64, 224
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 182
BfmI CTRYAG 1 cut(s) 325
BisI GCNGC 2 cut(s) 52, 328
BlsI GCNGC 2 cut(s) 53, 329
Bme1390I CCNGG 1 cut(s) 185
Bme18I GGWCC 2 cut(s) 103, 259
BmgT120I GGNCC 3 cut(s) 86, 103, 259
BmiI GGNNCC 4 cut(s) 88, 105, 169, 261
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 2 cut(s) 63, 234
BpuEI CTTGAG 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 2 cut(s) 82, 108
Bsc4I CCNNNNNNNGG 1 cut(s) 47
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 2 cut(s) 82, 108
BseGI GGATG 1 cut(s) 54
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMII CTCAG 2 cut(s) 139, 239
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 339
BsgI GTGCAG 1 cut(s) 61
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 3 cut(s) 5, 51, 87
BshNI GGYRCC 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 1 cut(s) 39
BslFI GGGAC 2 cut(s) 89, 245
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 89, 245
BsnI GGCC 3 cut(s) 5, 51, 87
Bso31I GGTCTC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 2 cut(s) 69, 228
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 4 cut(s) 52, 97, 148, 205
BspANI GGCC 3 cut(s) 5, 51, 87
BspCNI CTCAG 2 cut(s) 138, 238
BspLI GGNNCC 4 cut(s) 88, 105, 169, 261
BspMAI CTGCAG 1 cut(s) 329
BspPI GGATC 1 cut(s) 223
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 182
BsrBI CCGCTC 1 cut(s) 99
BssECI CCNNGG 2 cut(s) 82, 108
BssMI GATC 2 cut(s) 69, 228
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 185
Bst6I CTCTTC 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 133, 148
BstDEI CTNAG 2 cut(s) 125, 225
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 54
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 2 cut(s) 72, 231
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 2 cut(s) 69, 228
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 1 cut(s) 84
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 325
BstV1I GCAGC 1 cut(s) 339
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
BstZI CGGCCG 1 cut(s) 49
BsuRI GGCC 3 cut(s) 5, 51, 87
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 133, 148
CfoI GCGC 1 cut(s) 239
Cfr13I GGNCC 3 cut(s) 86, 103, 259
CviAII CATG 4 cut(s) 109, 136, 164, 214
CviJI RGCY 7 cut(s) 5, 37, 51, 87, 121, 131, 330
CviKI_1 RGCY 7 cut(s) 5, 37, 51, 87, 121, 131, 330
DdeI CTNAG 2 cut(s) 125, 225
DpnI GATC 2 cut(s) 71, 230
DpnII GATC 2 cut(s) 69, 228
EaeI YGGCCR 2 cut(s) 3, 49
EagI CGGCCG 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 37
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 2 cut(s) 103, 259
Eco52I CGGCCG 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 4 cut(s) 112, 139, 167, 217
FaqI GGGAC 2 cut(s) 89, 245
FatI CATG 4 cut(s) 108, 135, 163, 213
FauI CCCGC 2 cut(s) 104, 155
Fnu4HI GCNGC 2 cut(s) 52, 328
FokI GGATG 1 cut(s) 41
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 2 cut(s) 52, 328
GlaI GCGC 1 cut(s) 238
GluI GCNGC 2 cut(s) 52, 328
HaeIII GGCC 3 cut(s) 5, 51, 87
HhaI GCGC 1 cut(s) 239
Hin1II CATG 4 cut(s) 112, 139, 167, 217
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HindIII AAGCTT 1 cut(s) 119
Hpy188I TCNGA 1 cut(s) 228
Hpy188III TCNNGA 1 cut(s) 115
Hpy99I CGWCG 2 cut(s) 50, 146
HpyAV CCTTC 1 cut(s) 14
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 3 cut(s) 78, 217, 327
HpyF10VI GCNNNNNNNGC 1 cut(s) 84
HpyF3I CTNAG 2 cut(s) 125, 225
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 4 cut(s) 112, 139, 167, 217
HspAI GCGC 1 cut(s) 237
Kzo9I GATC 2 cut(s) 69, 228
LmnI GCTCC 2 cut(s) 34, 42
LpnPI CCDG 4 cut(s) 112, 117, 170, 197
Lsp1109I GCAGC 1 cut(s) 339
LweI GCATC 2 cut(s) 63, 234
MaeII ACGT 1 cut(s) 141
MalI GATC 2 cut(s) 71, 230
MbiI CCGCTC 1 cut(s) 99
MboI GATC 2 cut(s) 69, 228
MboII GAAGA 1 cut(s) 84
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 288, 296
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 6 cut(s) 92, 100, 203, 242, 292, 375
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 287
MslI CAYNNNNRTG 1 cut(s) 212
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MwoI GCNNNNNNNGC 1 cut(s) 84
NcoI CCATGG 1 cut(s) 108
NdeII GATC 2 cut(s) 69, 228
NlaIII CATG 4 cut(s) 112, 139, 167, 217
NlaIV GGNNCC 4 cut(s) 88, 105, 169, 261
NmeAIII GCCGAG 1 cut(s) 63
PkrI GCNGC 2 cut(s) 53, 329
PpuMI RGGWCCY 1 cut(s) 259
PshBI ATTAAT 1 cut(s) 287
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 4 cut(s) 88, 105, 169, 261
PspPI GGNCC 3 cut(s) 86, 103, 259
PspPPI RGGWCCY 1 cut(s) 259
PstI CTGCAG 1 cut(s) 329
PsuI RGATCY 1 cut(s) 228
RseI CAYNNNNRTG 1 cut(s) 212
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 2 cut(s) 52, 328
Sau3AI GATC 2 cut(s) 69, 228
Sau96I GGNCC 3 cut(s) 86, 103, 259
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 1 cut(s) 39
SetI ASST 5 cut(s) 39, 123, 144, 261, 332
SfaNI GCATC 2 cut(s) 63, 234
SfcI CTRYAG 1 cut(s) 325
SinI GGWCC 2 cut(s) 103, 259
SmiMI CAYNNNNRTG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 2 cut(s) 288, 296
SsiI CCGC 4 cut(s) 52, 97, 148, 205
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 108
TaiI ACGT 1 cut(s) 144
TaqI TCGA 3 cut(s) 114, 195, 249
TaqII GACCGA 1 cut(s) 91
TasI AATT 2 cut(s) 288, 296
TauI GCSGC 1 cut(s) 54
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 1 cut(s) 214
TseI GCWGC 1 cut(s) 327
TspDTI ATGAA 1 cut(s) 152
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 2 cut(s) 103, 259
VspI ATTAAT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.