Rh6CG468200

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0024709
Physical Location & Seq
Reverse (-)
63993582 .. 63993770
189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG468200.1

Sequence Viewer

Length: 189 bp
ATGAAGATCCTGTGCACCCTGGGACCTGCATCCAAGTCGGTGCCGATGGTGGAGAAGCTTCTCCGGGCCGGAATGAACGTGGCCCGCTTCAACTTCTTCCATGGATCCCACGAGTACCATCAGGAGACCCTCAATAATCTCAGGGCCGCCATGGATTCCACCGAAATTCTTAGCGTCGGCCTCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

62

Amino Acids

6.91

Weight (kDa)

8.0

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 2 - 56 1.4e-19 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024709)

Species Orthologous Gene IDs
pyrus_communis pycom2749g00010
rosa_chinensis RchiOBHm_Chr5g0044381
rosa_samantha Rh6CG468200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 34
AccB1I GGYRCC 1 cut(s) 40
AciI CCGC 2 cut(s) 85, 147
AclWI GGATC 2 cut(s) 99, 112
AcsI RAATTY 1 cut(s) 165
AfaI GTAC 1 cut(s) 116
AgsI TTSAA 1 cut(s) 91
AjnI CCWGG 1 cut(s) 18
AluBI AGCT 1 cut(s) 58
AluI AGCT 1 cut(s) 58
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 119
Alw44I GTGCAC 1 cut(s) 13
AlwI GGATC 2 cut(s) 99, 112
AoxI GGCC 4 cut(s) 66, 81, 144, 178
ApaLI GTGCAC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 165
AspS9I GGNCC 4 cut(s) 23, 66, 82, 144
AsuC2I CCSGG 1 cut(s) 65
AvaII GGWCC 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 17
BamHI GGATCC 1 cut(s) 104
BanI GGYRCC 1 cut(s) 40
BauI CACGAG 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 17
BccI CCATC 2 cut(s) 40, 126
BcgI CGANNNNNNTGC 2 cut(s) 18, 52
BciT130I CCWGG 1 cut(s) 20
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 119
BfuAI ACCTGC 1 cut(s) 34
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 2 cut(s) 20, 65
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 4 cut(s) 23, 66, 82, 144
BmiI GGNNCC 3 cut(s) 24, 42, 106
BmrFI CCNGG 2 cut(s) 20, 65
BmsI GCATC 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 4 cut(s) 18, 19, 100, 150
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 4 cut(s) 18, 19, 100, 150
BseGI GGATG 1 cut(s) 29
BseMII CTCAG 1 cut(s) 154
BseSI GKGCMC 1 cut(s) 17
BshFI GGCC 4 cut(s) 68, 83, 146, 180
BshNI GGYRCC 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 17
BsiSI CCGG 2 cut(s) 64, 69
BslFI GGGAC 1 cut(s) 36
BsmAI GTCTC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 4 cut(s) 68, 83, 146, 180
Bso31I GGTCTC 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 2 cut(s) 6, 104
Bsp19I CCATGG 2 cut(s) 100, 150
BspACI CCGC 2 cut(s) 85, 147
BspANI GGCC 4 cut(s) 68, 83, 146, 180
BspCNI CTCAG 1 cut(s) 153
BspLI GGNNCC 3 cut(s) 24, 42, 106
BspMI ACCTGC 1 cut(s) 34
BspPI GGATC 2 cut(s) 99, 112
BspT107I GGYRCC 1 cut(s) 40
BspTNI GGTCTC 1 cut(s) 119
BssECI CCNNGG 4 cut(s) 18, 19, 100, 150
BssMI GATC 2 cut(s) 6, 104
BssSI CACGAG 1 cut(s) 110
BssT1I CCWWGG 2 cut(s) 100, 150
Bst2BI CACGAG 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 20
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 2 cut(s) 140, 170
BstDSI CCRYGG 2 cut(s) 100, 150
BstF5I GGATG 1 cut(s) 29
BstKTI GATC 2 cut(s) 9, 107
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 2 cut(s) 6, 104
BstNI CCWGG 1 cut(s) 20
BstSCI CCNGG 2 cut(s) 18, 63
BstSLI GKGCMC 1 cut(s) 17
BstX2I RGATCY 2 cut(s) 6, 104
BstYI RGATCY 2 cut(s) 6, 104
BsuRI GGCC 4 cut(s) 68, 83, 146, 180
BtgI CCRYGG 2 cut(s) 100, 150
BtsCI GGATG 1 cut(s) 29
BveI ACCTGC 1 cut(s) 34
Cac8I GCNNGC 1 cut(s) 85
Cfr13I GGNCC 4 cut(s) 23, 66, 82, 144
CseI GACGC 1 cut(s) 163
Csp6I GTAC 1 cut(s) 115
CviAII CATG 2 cut(s) 101, 151
CviJI RGCY 5 cut(s) 58, 68, 83, 146, 180
CviKI_1 RGCY 5 cut(s) 58, 68, 83, 146, 180
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 2 cut(s) 140, 170
DpnI GATC 2 cut(s) 8, 106
DpnII GATC 2 cut(s) 6, 104
Eco130I CCWWGG 2 cut(s) 100, 150
Eco31I GGTCTC 1 cut(s) 119
Eco47I GGWCC 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 18
EcoT14I CCWWGG 2 cut(s) 100, 150
ErhI CCWWGG 2 cut(s) 100, 150
FaeI CATG 2 cut(s) 104, 154
FaiI YATR 2 cut(s) 102, 152
FaqI GGGAC 1 cut(s) 36
FatI CATG 2 cut(s) 100, 150
FauI CCCGC 1 cut(s) 92
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 1 cut(s) 16
Fsp4HI GCNGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 147
HaeIII GGCC 4 cut(s) 68, 83, 146, 180
HapII CCGG 2 cut(s) 64, 69
HgaI GACGC 1 cut(s) 163
Hin1II CATG 2 cut(s) 104, 154
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 1 cut(s) 155
HpaII CCGG 2 cut(s) 64, 69
Hpy166II GTNNAC 1 cut(s) 15
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 1 cut(s) 15
Hpy99I CGWCG 1 cut(s) 179
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 2 cut(s) 15, 29
HpyF3I CTNAG 2 cut(s) 140, 170
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 2 cut(s) 104, 154
Kzo9I GATC 2 cut(s) 6, 104
LpnPI CCDG 8 cut(s) 5, 23, 32, 39, 77, 82, 107, 127
LweI GCATC 1 cut(s) 38
MaeII ACGT 1 cut(s) 78
MalI GATC 2 cut(s) 8, 106
MboI GATC 2 cut(s) 6, 104
MboII GAAGA 2 cut(s) 16, 88
MflI RGATCY 2 cut(s) 6, 104
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 1 cut(s) 165
MnlI CCTC 1 cut(s) 140
MspI CCGG 2 cut(s) 64, 69
MspR9I CCNGG 2 cut(s) 20, 65
MvaI CCWGG 1 cut(s) 20
NciI CCSGG 1 cut(s) 65
NcoI CCATGG 2 cut(s) 100, 150
NdeII GATC 2 cut(s) 6, 104
NlaIII CATG 2 cut(s) 104, 154
NlaIV GGNNCC 3 cut(s) 24, 42, 106
PasI CCCWGGG 1 cut(s) 19
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 1 cut(s) 148
PpuMI RGGWCCY 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
PspN4I GGNNCC 3 cut(s) 24, 42, 106
PspPI GGNCC 4 cut(s) 23, 66, 82, 144
PspPPI RGGWCCY 1 cut(s) 23
PsuI RGATCY 2 cut(s) 6, 104
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 2 cut(s) 6, 104
Sau96I GGNCC 4 cut(s) 23, 66, 82, 144
ScrFI CCNGG 2 cut(s) 20, 65
SduI GDGCHC 1 cut(s) 17
SetI ASST 3 cut(s) 28, 60, 81
SfaNI GCATC 1 cut(s) 38
SinI GGWCC 1 cut(s) 23
Sse9I AATT 1 cut(s) 165
SsiI CCGC 2 cut(s) 85, 147
StyD4I CCNGG 2 cut(s) 18, 63
StyI CCWWGG 2 cut(s) 100, 150
TaiI ACGT 1 cut(s) 81
TasI AATT 1 cut(s) 165
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 1 cut(s) 155
TspDTI ATGAA 2 cut(s) 17, 89
VneI GTGCAC 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 23
XapI RAATTY 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.