RchiOBHm_Chr5g0044381

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0024709
Physical Location & Seq
Reverse (-)
39692639 .. 39692899
261 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32259

Sequence Viewer

Length: 261 bp
ATGAATTCAAAGATTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGAGAGAGAGAGAGACAAAGATCCTGTGCACCCTGGGACCTGCATCCAGGTCGGTGCCGATGGTGGAGAAGCTTCGCCGGGCCGGAATGAATGTGGCCCGCTTCAACTTCTTCCATGGATCCCACAAGTACCATCAGGAGACCCTCACTAATCTCAGGGCTGCCATGGATTCCACCGAAATTCTCAGCGCCGCCCTCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

86

Amino Acids

10.01

Weight (kDa)

4.81

Isoelectric Point (pI)

103.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 23 - 81 7.3e-20 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024709)

Species Orthologous Gene IDs
pyrus_communis pycom2749g00010
rosa_chinensis RchiOBHm_Chr5g0044381
rosa_samantha Rh6CG468200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 112
AciI CCGC 2 cut(s) 157, 249
AclWI GGATC 3 cut(s) 73, 171, 184
AcsI RAATTY 2 cut(s) 4, 237
AfaI GTAC 1 cut(s) 188
AgsI TTSAA 3 cut(s) 9, 17, 163
AjnI CCWGG 2 cut(s) 90, 104
AluBI AGCT 1 cut(s) 130
AluI AGCT 1 cut(s) 130
Alw21I GWGCWC 1 cut(s) 89
Alw26I GTCTC 2 cut(s) 65, 191
Alw44I GTGCAC 1 cut(s) 85
AlwI GGATC 3 cut(s) 73, 171, 184
AoxI GGCC 2 cut(s) 138, 153
ApaLI GTGCAC 1 cut(s) 85
ApeKI GCWGC 1 cut(s) 218
ApoI RAATTY 2 cut(s) 4, 237
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 3 cut(s) 95, 138, 154
AsuC2I CCSGG 1 cut(s) 137
AvaII GGWCC 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 89
BamHI GGATCC 1 cut(s) 176
BanI GGYRCC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 89
BbvI GCAGC 1 cut(s) 205
BccI CCATC 2 cut(s) 112, 198
BcgI CGANNNNNNTGC 2 cut(s) 90, 124
BciT130I CCWGG 2 cut(s) 92, 106
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 2 cut(s) 65, 191
BfoI RGCGCY 1 cut(s) 249
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 2 cut(s) 219, 249
BlsI GCNGC 2 cut(s) 220, 250
Bme1390I CCNGG 3 cut(s) 92, 106, 137
Bme18I GGWCC 1 cut(s) 95
BmgT120I GGNCC 3 cut(s) 95, 138, 154
BmiI GGNNCC 3 cut(s) 96, 114, 178
BmrFI CCNGG 3 cut(s) 92, 106, 137
BmsI GCATC 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 4 cut(s) 90, 91, 172, 222
BseBI CCWGG 2 cut(s) 92, 106
BseDI CCNNGG 4 cut(s) 90, 91, 172, 222
BseGI GGATG 1 cut(s) 101
BseMII CTCAG 2 cut(s) 226, 256
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 1 cut(s) 205
BshFI GGCC 2 cut(s) 140, 155
BshNI GGYRCC 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 89
BsiSI CCGG 2 cut(s) 136, 141
BslFI GGGAC 1 cut(s) 108
BsmAI GTCTC 2 cut(s) 65, 191
BsmFI GGGAC 1 cut(s) 108
BsnI GGCC 2 cut(s) 140, 155
Bso31I GGTCTC 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 89
Bsp143I GATC 2 cut(s) 78, 176
Bsp19I CCATGG 2 cut(s) 172, 222
BspACI CCGC 2 cut(s) 157, 249
BspANI GGCC 2 cut(s) 140, 155
BspCNI CTCAG 2 cut(s) 225, 255
BspLI GGNNCC 3 cut(s) 96, 114, 178
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 3 cut(s) 73, 171, 184
BspT107I GGYRCC 1 cut(s) 112
BspTNI GGTCTC 1 cut(s) 191
BssECI CCNNGG 4 cut(s) 90, 91, 172, 222
BssMI GATC 2 cut(s) 78, 176
BssT1I CCWWGG 2 cut(s) 172, 222
Bst2UI CCWGG 2 cut(s) 92, 106
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 2 cut(s) 212, 242
BstDSI CCRYGG 2 cut(s) 172, 222
BstF5I GGATG 1 cut(s) 101
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 2 cut(s) 81, 179
BstMAI GTCTC 2 cut(s) 65, 191
BstMBI GATC 2 cut(s) 78, 176
BstNI CCWGG 2 cut(s) 92, 106
BstSCI CCNGG 3 cut(s) 90, 104, 135
BstSLI GKGCMC 1 cut(s) 89
BstV1I GCAGC 1 cut(s) 205
BstX2I RGATCY 2 cut(s) 78, 176
BstYI RGATCY 2 cut(s) 78, 176
BsuRI GGCC 2 cut(s) 140, 155
BtgI CCRYGG 2 cut(s) 172, 222
BtsCI GGATG 1 cut(s) 101
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 1 cut(s) 157
CfoI GCGC 1 cut(s) 248
Cfr13I GGNCC 3 cut(s) 95, 138, 154
Csp6I GTAC 1 cut(s) 187
CviAII CATG 2 cut(s) 173, 223
CviJI RGCY 4 cut(s) 130, 140, 155, 218
CviKI_1 RGCY 4 cut(s) 130, 140, 155, 218
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 2 cut(s) 212, 242
DpnI GATC 2 cut(s) 80, 178
DpnII GATC 2 cut(s) 78, 176
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 172, 222
Eco31I GGTCTC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 2 cut(s) 90, 104
EcoT14I CCWWGG 2 cut(s) 172, 222
ErhI CCWWGG 2 cut(s) 172, 222
FaeI CATG 2 cut(s) 176, 226
FaiI YATR 2 cut(s) 174, 224
FaqI GGGAC 1 cut(s) 108
FatI CATG 2 cut(s) 172, 222
FauI CCCGC 1 cut(s) 164
Fnu4HI GCNGC 2 cut(s) 219, 249
FokI GGATG 1 cut(s) 88
Fsp4HI GCNGC 2 cut(s) 219, 249
GlaI GCGC 1 cut(s) 247
GluI GCNGC 2 cut(s) 219, 249
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 2 cut(s) 140, 155
HapII CCGG 2 cut(s) 136, 141
HhaI GCGC 1 cut(s) 248
Hin1II CATG 2 cut(s) 176, 226
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 1 cut(s) 227
HpaII CCGG 2 cut(s) 136, 141
Hpy166II GTNNAC 1 cut(s) 87
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 1 cut(s) 87
HpyCH4V TGCA 2 cut(s) 87, 101
HpyF3I CTNAG 2 cut(s) 212, 242
Hsp92II CATG 2 cut(s) 176, 226
HspAI GCGC 1 cut(s) 246
Kzo9I GATC 2 cut(s) 78, 176
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 1 cut(s) 110
MalI GATC 2 cut(s) 80, 178
MboI GATC 2 cut(s) 78, 176
MflI RGATCY 2 cut(s) 78, 176
MhlI GDGCHC 1 cut(s) 89
MluCI AATT 2 cut(s) 4, 237
MnlI CCTC 1 cut(s) 212
MspI CCGG 2 cut(s) 136, 141
MspR9I CCNGG 3 cut(s) 92, 106, 137
MvaI CCWGG 2 cut(s) 92, 106
NciI CCSGG 1 cut(s) 137
NcoI CCATGG 2 cut(s) 172, 222
NdeII GATC 2 cut(s) 78, 176
NlaIII CATG 2 cut(s) 176, 226
NlaIV GGNNCC 3 cut(s) 96, 114, 178
PasI CCCWGGG 1 cut(s) 91
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 220, 250
PpuMI RGGWCCY 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 95
Psp6I CCWGG 2 cut(s) 90, 104
PspGI CCWGG 2 cut(s) 90, 104
PspN4I GGNNCC 3 cut(s) 96, 114, 178
PspPI GGNCC 3 cut(s) 95, 138, 154
PspPPI RGGWCCY 1 cut(s) 95
PsuI RGATCY 2 cut(s) 78, 176
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SatI GCNGC 2 cut(s) 219, 249
Sau3AI GATC 2 cut(s) 78, 176
Sau96I GGNCC 3 cut(s) 95, 138, 154
ScrFI CCNGG 3 cut(s) 92, 106, 137
SduI GDGCHC 1 cut(s) 89
SetI ASST 3 cut(s) 100, 110, 132
SfaNI GCATC 1 cut(s) 110
SinI GGWCC 1 cut(s) 95
Sse9I AATT 2 cut(s) 4, 237
SsiI CCGC 2 cut(s) 157, 249
StyD4I CCNGG 3 cut(s) 90, 104, 135
StyI CCWWGG 2 cut(s) 172, 222
TasI AATT 2 cut(s) 4, 237
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 1 cut(s) 227
TseI GCWGC 1 cut(s) 218
TspDTI ATGAA 2 cut(s) 17, 161
VneI GTGCAC 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 95
XapI RAATTY 2 cut(s) 4, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.