RCHIOBHM_CPG0501871

30S ribosomal protein S8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Pt
OG0020235
Physical Location & Seq
Forward (+)
30943 .. 31579
637 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 405 bp
ATGAGTAAAGACACTATTGCTGACATAATAACCTCTATACGAAATGCTGACATGAATGAAAAAAGAACAGTTCGAATACCATCTACTAACATGACTGAAAATATTGTTAAAATCCTTTTACGAGAAGGTTTTATTGAAAACGTGAGAAAACATCAGGAAAGCAATAAATATTTTTTGGTTTTAACCCTACGACATAGGAGAAATAGGAAAGGATCATATAGAACTGTTTTAAATTTAAAACGGATCAGCCGGCCCGGCCTACGAATTTATTCTAACTATCAAGGAATTCCGAGAATTTTAGGCGGAATGGGGATTGTAATTCTTTCTACTTCTCGAGGGATAATGACAGACCGAGAGGCTCGAATAGAAGGAATCGGCGGGGAAATTTTGTGTTATATATGGTAA

Protein Analysis

134

Amino Acids

15.49

Weight (kDa)

10.56

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8 PF00410 4 - 134 6.3e-43 Ribosomal protein S8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0020235)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00770
rosa_chinensis RchiOBHm_CPg0501871
rosa_roxburghii Rroxscaffold_2G00119000
rosa_samantha Rh1CG032600
rosa_wichuraiana Rw7G013540 Rw7G019280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 303, 378
AclWI GGATC 2 cut(s) 220, 251
AcsI RAATTY 5 cut(s) 232, 264, 285, 294, 384
AgsI TTSAA 1 cut(s) 137
AlwI GGATC 2 cut(s) 220, 251
Ama87I CYCGRG 1 cut(s) 333
AoxI GGCC 2 cut(s) 251, 256
ApoI RAATTY 5 cut(s) 232, 264, 285, 294, 384
Asp700I GAANNNNTTC 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 252
AsuC2I CCSGG 1 cut(s) 255
AsuII TTCGAA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 333
BccI CCATC 1 cut(s) 88
BcnI CCSGG 1 cut(s) 255
BglI GCCNNNNNGGC 1 cut(s) 255
Bme1390I CCNGG 1 cut(s) 255
BmeT110I CYCGRG 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 252
BmrFI CCNGG 1 cut(s) 255
Bpu14I TTCGAA 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 255
Bse118I RCCGGY 1 cut(s) 249
BshFI GGCC 2 cut(s) 253, 258
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 2 cut(s) 250, 255
BsnI GGCC 2 cut(s) 253, 258
BsoBI CYCGRG 1 cut(s) 333
Bsp119I TTCGAA 1 cut(s) 73
Bsp143I GATC 2 cut(s) 212, 243
BspACI CCGC 2 cut(s) 303, 378
BspANI GGCC 2 cut(s) 253, 258
BspPI GGATC 2 cut(s) 220, 251
BspT104I TTCGAA 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 249
BssAI RCCGGY 1 cut(s) 249
BssMI GATC 2 cut(s) 212, 243
Bst4CI ACNGT 2 cut(s) 70, 226
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 1 cut(s) 251
BstKTI GATC 2 cut(s) 215, 246
BstMBI GATC 2 cut(s) 212, 243
BstMWI GCNNNNNNNGC 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 253
BsuRI GGCC 2 cut(s) 253, 258
Cac8I GCNNGC 1 cut(s) 251
Cfr10I RCCGGY 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 252
CviAII CATG 2 cut(s) 52, 91
CviJI RGCY 4 cut(s) 249, 253, 258, 359
CviKI_1 RGCY 4 cut(s) 249, 253, 258, 359
DpnI GATC 2 cut(s) 214, 245
DpnII GATC 2 cut(s) 212, 243
DraI TTTAAA 2 cut(s) 231, 237
EciI GGCGGA 1 cut(s) 318
Eco88I CYCGRG 1 cut(s) 333
EcoRI GAATTC 1 cut(s) 285
FaeI CATG 2 cut(s) 55, 94
FatI CATG 2 cut(s) 51, 90
FauI CCCGC 1 cut(s) 371
HaeIII GGCC 2 cut(s) 253, 258
HapII CCGG 2 cut(s) 250, 255
Hin1II CATG 2 cut(s) 55, 94
HinfI GANTC 1 cut(s) 372
HpaII CCGG 2 cut(s) 250, 255
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 2 cut(s) 155, 333
HpyAV CCTTC 2 cut(s) 119, 362
HpyCH4III ACNGT 2 cut(s) 70, 226
HpyCH4IV ACGT 1 cut(s) 141
HpyF10VI GCNNNNNNNGC 1 cut(s) 255
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 2 cut(s) 55, 94
KroI GCCGGC 1 cut(s) 249
KroNI GCCGGC 1 cut(s) 251
Kzo9I GATC 2 cut(s) 212, 243
LpnPI CCDG 3 cut(s) 140, 263, 268
MaeII ACGT 1 cut(s) 141
MalI GATC 2 cut(s) 214, 245
MboI GATC 2 cut(s) 212, 243
MluCI AATT 6 cut(s) 232, 264, 285, 294, 318, 384
MnlI CCTC 3 cut(s) 43, 329, 349
MroNI GCCGGC 1 cut(s) 249
MroXI GAANNNNTTC 1 cut(s) 268
MseI TTAA 4 cut(s) 108, 182, 230, 236
MspI CCGG 2 cut(s) 250, 255
MspR9I CCNGG 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 255
NaeI GCCGGC 1 cut(s) 251
NciI CCSGG 1 cut(s) 255
NdeII GATC 2 cut(s) 212, 243
NgoMIV GCCGGC 1 cut(s) 249
NlaIII CATG 2 cut(s) 55, 94
NspV TTCGAA 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 333
PdiI GCCGGC 1 cut(s) 251
PdmI GAANNNNTTC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 372
PspPI GGNCC 1 cut(s) 252
SaqAI TTAA 4 cut(s) 108, 182, 230, 236
Sau3AI GATC 2 cut(s) 212, 243
Sau96I GGNCC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 255
SetI ASST 3 cut(s) 35, 130, 144
Sfr274I CTCGAG 1 cut(s) 333
SfuI TTCGAA 1 cut(s) 73
SlaI CTCGAG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 333
SmoI CTYRAG 1 cut(s) 333
Sse9I AATT 6 cut(s) 232, 264, 285, 294, 318, 384
SsiI CCGC 2 cut(s) 303, 378
SspI AATATT 2 cut(s) 103, 170
StyD4I CCNGG 1 cut(s) 253
TaaI ACNGT 2 cut(s) 70, 226
TaiI ACGT 1 cut(s) 144
TaqI TCGA 3 cut(s) 73, 334, 361
TaqII GACCGA 1 cut(s) 366
TasI AATT 6 cut(s) 232, 264, 285, 294, 318, 384
TfiI GAWTC 1 cut(s) 372
Tru1I TTAA 4 cut(s) 108, 182, 230, 236
Tru9I TTAA 4 cut(s) 108, 182, 230, 236
TspDTI ATGAA 2 cut(s) 68, 72
TspGWI ACGGA 1 cut(s) 256
XapI RAATTY 5 cut(s) 232, 264, 285, 294, 384
XhoI CTCGAG 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.