RchiOBHm_Chr6g0289121

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0025096
Physical Location & Seq
Reverse (-)
52313138 .. 52314475
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1155 bp
ATGTTTCTCGAGCTGTTAGCCTTCTCCTCCCTACTCATCCTCCCCTTGCTTTTCTTCATCCTCAAGCTTTCCGGCAACTCGAAAACCAACAGCAACAGCAAGCTCCCCAAATCATACCCAATAATCAGTTCATTCCCAGCCCTCTACAAGAACTATGCCAATAGACTCCCATGGTTCACTGAGCTTCTCCAAGACTCGCCCACATCTACTATTACTATGCACCATTCTCTGGGCTGCCAGCCTGCCACTTCGTCGTCACCGCCAACTCGGCCGTGGTCCAGCACATCCTCAAGACAAAGTTCCACATCTACCAAAAGGGCGAGTCCTTCCGTACAACTCTCCACGACCTCCTTGGCGATGGGATTTTCAACGCCGACGGTGACAATTGGAAGTTCCAATCTTCCAGCGACCGAACCATGCATTCTCGATTTCCAAGACATTCTCCAGCGGTTCGCCTTCGACAACATTTGCCGGATCGGGTTCGGGTACGACCCGGAGTATCTCCTTCCTTCTCTGCCGCAAGCTAAATTCGCGGTGGCGTTCGACAATGAGGTCCAGATCAGCAGCGAAAGGTTCAGTTCCACGCGCCCGGTGTGGAAGATCAAACGAGCTCTCGGAATCGGATCGGAAAGCCATCTCCACTGTGTGGTCTCTAACGTCCGAGAGTTTGCGAACACCACCGTCAAGCAAAAGAAGCATGAAATGGACGAGGAGAAGACTCTGGAGTCTCATGATCTGCTGTCTAGGTTCTTGAGCACCGGCCATTCCGATGAGAAATTCGTCACTGACATCGTCATCAGCTTCACTCTCGCCGGAAGGGACACGACCTCGGCGGCTTTGACCTGGTTCTTCTGGCTAATCTCAAACCATCCAAAAGTCGAAAACCAAATTCTCAAGGAGATCAATGAGAAAACGGAGACCGGAGCCAGATATGATGAAGCGAAGGAGATGGTGTACACACACGTGGCGATCTGTGAGAGCATGAGGCTGTACCCGCCGGTTCCGACTGACAACAAAGAGGCGGTAGAAGACGACGTTTTGCCGGACGGGACTAAAGTGAAGAATGGGACGACAGTGACGTACCACGTGTACGTGATGGGGCGGATGGAGCAGATATGGGGCAAGGATTGGGCCGAGTTCCGTCCCGAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

384

Amino Acids

43.21

Weight (kDa)

6.38

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 142 - 384 1.2e-31 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025096)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0289121
rosa_samantha Rh6CG325400 Rh6DG311300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 551, 724
AccB7I CCANNNNNTGG 2 cut(s) 229, 646
AccII CGCG 2 cut(s) 533, 586
AciI CCGC 8 cut(s) 260, 448, 518, 533, 833, 995, 1022, 1102
AclWI GGATC 2 cut(s) 482, 631
AcoI YGGCCR 2 cut(s) 269, 760
AcsI RAATTY 3 cut(s) 527, 776, 888
AcvI CACGTG 2 cut(s) 964, 1087
AdeI CACNNNGTG 1 cut(s) 646
AfaI GTAC 6 cut(s) 333, 488, 956, 992, 1082, 1091
AfiI CCNNNNNNNGG 2 cut(s) 229, 646
AflIII ACRYGT 2 cut(s) 961, 1086
AgsI TTSAA 1 cut(s) 369
AjnI CCWGG 1 cut(s) 842
AjuI GAANNNNNNNTTGG 2 cut(s) 112, 144
AleI CACNNNNGTG 1 cut(s) 962
AluBI AGCT 7 cut(s) 13, 67, 103, 184, 524, 611, 801
AluI AGCT 7 cut(s) 13, 67, 103, 184, 524, 611, 801
Alw21I GWGCWC 2 cut(s) 613, 758
Alw26I GTCTC 3 cut(s) 655, 732, 911
AlwI GGATC 2 cut(s) 482, 631
Ama87I CYCGRG 2 cut(s) 8, 1145
AoxI GGCC 3 cut(s) 269, 760, 1131
ApeKI GCWGC 2 cut(s) 234, 564
ApoI RAATTY 3 cut(s) 527, 776, 888
AspLEI GCGC 1 cut(s) 588
AspS9I GGNCC 3 cut(s) 276, 553, 1131
AsuC2I CCSGG 2 cut(s) 494, 590
AsuHPI GGTGA 2 cut(s) 249, 391
AvaI CYCGRG 2 cut(s) 8, 1145
AvaII GGWCC 2 cut(s) 276, 553
BanII GRGCYC 1 cut(s) 613
BbrPI CACGTG 2 cut(s) 964, 1087
BbsI GAAGAC 2 cut(s) 722, 1035
Bbv12I GWGCWC 2 cut(s) 613, 758
BbvI GCAGC 2 cut(s) 221, 576
BccI CCATC 6 cut(s) 352, 642, 876, 943, 1090, 1099
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 1 cut(s) 844
BcnI CCSGG 2 cut(s) 494, 590
BcoDI GTCTC 3 cut(s) 655, 732, 911
BfaI CTAG 1 cut(s) 744
BglI GCCNNNNNGGC 1 cut(s) 268
BisI GCNGC 4 cut(s) 235, 518, 565, 834
BlsI GCNGC 4 cut(s) 236, 519, 566, 835
Bme1390I CCNGG 3 cut(s) 494, 590, 844
Bme18I GGWCC 2 cut(s) 276, 553
BmeT110I CYCGRG 2 cut(s) 8, 1145
BmgT120I GGNCC 3 cut(s) 276, 553, 1131
BmiI GGNNCC 2 cut(s) 925, 1002
BmrFI CCNGG 3 cut(s) 494, 590, 844
BpiI GAAGAC 2 cut(s) 722, 1035
BpmI CTGGAG 2 cut(s) 428, 743
BpuEI CTTGAG 4 cut(s) 47, 274, 772, 878
BpuMI CCSGG 2 cut(s) 494, 590
BsaAI YACGTR 3 cut(s) 964, 1087, 1093
BsaI GGTCTC 2 cut(s) 655, 911
BsaJI CCNNGG 4 cut(s) 170, 272, 351, 828
BsaWI WCCGGW 1 cut(s) 920
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 938, 968
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 646
Bse118I RCCGGY 2 cut(s) 758, 997
BseBI CCWGG 1 cut(s) 844
BseDI CCNNGG 4 cut(s) 170, 272, 351, 828
BseGI GGATG 5 cut(s) 36, 57, 284, 868, 1110
BseLI CCNNNNNNNGG 2 cut(s) 229, 646
BseMII CTCAG 1 cut(s) 171
BseRI GAGGAG 2 cut(s) 16, 725
BseX3I CGGCCG 1 cut(s) 269
BseXI GCAGC 2 cut(s) 221, 576
BseYI CCCAGC 1 cut(s) 136
Bsh1236I CGCG 2 cut(s) 533, 586
Bsh1285I CGRYCG 2 cut(s) 272, 411
BshFI GGCC 3 cut(s) 271, 762, 1133
BsiEI CGRYCG 2 cut(s) 272, 411
BsiHKAI GWGCWC 2 cut(s) 613, 758
BsiHKCI CYCGRG 2 cut(s) 8, 1145
BsiSI CCGG 9 cut(s) 72, 472, 494, 590, 759, 813, 921, 998, 1043
BslFI GGGAC 4 cut(s) 833, 1063, 1081, 1128
BslI CCNNNNNNNGG 2 cut(s) 229, 646
BsmAI GTCTC 3 cut(s) 655, 732, 911
BsmFI GGGAC 4 cut(s) 833, 1063, 1081, 1128
BsmI GAATGC 1 cut(s) 420
BsnI GGCC 3 cut(s) 271, 762, 1133
Bso31I GGTCTC 2 cut(s) 655, 911
BsoBI CYCGRG 2 cut(s) 8, 1145
Bsp1286I GDGCHC 2 cut(s) 613, 758
Bsp1407I TGTACA 1 cut(s) 954
Bsp143I GATC 7 cut(s) 474, 558, 600, 623, 733, 900, 969
Bsp19I CCATGG 1 cut(s) 170
BspACI CCGC 8 cut(s) 260, 448, 518, 533, 833, 995, 1022, 1102
BspANI GGCC 3 cut(s) 271, 762, 1133
BspCNI CTCAG 1 cut(s) 172
BspFNI CGCG 2 cut(s) 533, 586
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 2 cut(s) 925, 1002
BspPI GGATC 2 cut(s) 482, 631
BspTNI GGTCTC 2 cut(s) 655, 911
BsrFI RCCGGY 2 cut(s) 758, 997
BsrGI TGTACA 1 cut(s) 954
BssAI RCCGGY 2 cut(s) 758, 997
BssECI CCNNGG 4 cut(s) 170, 272, 351, 828
BssMI GATC 7 cut(s) 474, 558, 600, 623, 733, 900, 969
BssT1I CCWWGG 2 cut(s) 170, 351
Bst2UI CCWGG 1 cut(s) 844
Bst4CI ACNGT 4 cut(s) 379, 644, 682, 1075
BstAUI TGTACA 1 cut(s) 954
BstBAI YACGTR 3 cut(s) 964, 1087, 1093
BstC8I GCNNGC 4 cut(s) 101, 239, 243, 522
BstDEI CTNAG 1 cut(s) 180
BstDSI CCRYGG 2 cut(s) 170, 272
BstF5I GGATG 5 cut(s) 36, 57, 284, 868, 1110
BstFNI CGCG 2 cut(s) 533, 586
BstHHI GCGC 1 cut(s) 588
BstKTI GATC 7 cut(s) 477, 561, 603, 626, 736, 903, 972
BstMAI GTCTC 3 cut(s) 655, 732, 911
BstMBI GATC 7 cut(s) 474, 558, 600, 623, 733, 900, 969
BstMCI CGRYCG 2 cut(s) 272, 411
BstMWI GCNNNNNNNGC 5 cut(s) 268, 530, 694, 994, 1108
BstNI CCWGG 1 cut(s) 844
BstSCI CCNGG 3 cut(s) 492, 588, 842
BstUI CGCG 2 cut(s) 533, 586
BstV1I GCAGC 2 cut(s) 221, 576
BstV2I GAAGAC 2 cut(s) 722, 1035
BstZI CGGCCG 1 cut(s) 269
BsuRI GGCC 3 cut(s) 271, 762, 1133
BtgI CCRYGG 2 cut(s) 170, 272
BtgZI GCGATG 1 cut(s) 371
BtsCI GGATG 5 cut(s) 36, 57, 284, 868, 1110
BtsIMutI CAGTG 4 cut(s) 177, 640, 783, 1080
Cac8I GCNNGC 4 cut(s) 101, 239, 243, 522
CciI TCATGA 1 cut(s) 730
CfoI GCGC 1 cut(s) 588
Cfr10I RCCGGY 2 cut(s) 758, 997
Cfr13I GGNCC 3 cut(s) 276, 553, 1131
CsiI ACCWGGT 1 cut(s) 842
Csp6I GTAC 6 cut(s) 332, 487, 955, 991, 1081, 1090
CviAII CATG 5 cut(s) 171, 417, 698, 731, 982
CviQI GTAC 6 cut(s) 332, 487, 955, 991, 1081, 1090
DdeI CTNAG 1 cut(s) 180
DpnI GATC 7 cut(s) 476, 560, 602, 625, 735, 902, 971
DpnII GATC 7 cut(s) 474, 558, 600, 623, 733, 900, 969
DraIII CACNNNGTG 1 cut(s) 646
DrdI GACNNNNNNGTC 2 cut(s) 551, 724
DseDI GACNNNNNNGTC 2 cut(s) 551, 724
EaeI YGGCCR 2 cut(s) 269, 760
EagI CGGCCG 1 cut(s) 269
EciI GGCGGA 1 cut(s) 1117
Ecl136II GAGCTC 1 cut(s) 611
EclXI CGGCCG 1 cut(s) 269
Eco130I CCWWGG 2 cut(s) 170, 351
Eco24I GRGCYC 1 cut(s) 613
Eco31I GGTCTC 2 cut(s) 655, 911
Eco47I GGWCC 2 cut(s) 276, 553
Eco52I CGGCCG 1 cut(s) 269
Eco53kI GAGCTC 1 cut(s) 611
Eco72I CACGTG 2 cut(s) 964, 1087
Eco88I CYCGRG 2 cut(s) 8, 1145
EcoICRI GAGCTC 1 cut(s) 611
EcoRII CCWGG 1 cut(s) 842
EcoT14I CCWWGG 2 cut(s) 170, 351
EcoT22I ATGCAT 1 cut(s) 422
EcoT38I GRGCYC 1 cut(s) 613
ErhI CCWWGG 2 cut(s) 170, 351
FaeI CATG 5 cut(s) 174, 420, 701, 734, 985
FaqI GGGAC 4 cut(s) 833, 1063, 1081, 1128
FatI CATG 5 cut(s) 170, 416, 697, 730, 981
FauI CCCGC 1 cut(s) 1002
Fnu4HI GCNGC 4 cut(s) 235, 518, 565, 834
FokI GGATG 5 cut(s) 23, 44, 271, 855, 1117
FriOI GRGCYC 1 cut(s) 613
Fsp4HI GCNGC 4 cut(s) 235, 518, 565, 834
FspBI CTAG 1 cut(s) 744
GlaI GCGC 1 cut(s) 587
GluI GCNGC 4 cut(s) 235, 518, 565, 834
GsaI CCCAGC 1 cut(s) 140
GsuI CTGGAG 2 cut(s) 428, 743
HaeIII GGCC 3 cut(s) 271, 762, 1133
HapII CCGG 9 cut(s) 72, 472, 494, 590, 759, 813, 921, 998, 1043
HhaI GCGC 1 cut(s) 588
Hin1II CATG 5 cut(s) 174, 420, 701, 734, 985
Hin6I GCGC 1 cut(s) 586
HinP1I GCGC 1 cut(s) 586
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 6 cut(s) 165, 194, 322, 618, 718, 725
HpaII CCGG 9 cut(s) 72, 472, 494, 590, 759, 813, 921, 998, 1043
HphI GGTGA 2 cut(s) 249, 391
Hpy166II GTNNAC 4 cut(s) 177, 955, 957, 1090
Hpy188I TCNGA 6 cut(s) 617, 623, 628, 662, 769, 1005
Hpy188III TCNNGA 8 cut(s) 8, 291, 425, 556, 722, 731, 751, 1145
Hpy8I GTNNAC 4 cut(s) 177, 955, 957, 1090
Hpy99I CGWCG 3 cut(s) 256, 379, 1037
HpyAV CCTTC 7 cut(s) 31, 336, 466, 515, 519, 810, 937
HpyCH4III ACNGT 4 cut(s) 379, 644, 682, 1075
HpyCH4IV ACGT 6 cut(s) 657, 963, 1035, 1079, 1086, 1092
HpyCH4V TGCA 2 cut(s) 220, 420
HpyF10VI GCNNNNNNNGC 5 cut(s) 268, 530, 694, 994, 1108
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 6 cut(s) 657, 963, 1035, 1079, 1086, 1092
Hsp92II CATG 5 cut(s) 174, 420, 701, 734, 985
HspAI GCGC 1 cut(s) 586
Kzo9I GATC 7 cut(s) 474, 558, 600, 623, 733, 900, 969
LmnI GCTCC 3 cut(s) 108, 923, 1108
Lsp1109I GCAGC 2 cut(s) 221, 576
MabI ACCWGGT 1 cut(s) 842
MaeI CTAG 1 cut(s) 744
MaeII ACGT 6 cut(s) 657, 963, 1035, 1079, 1086, 1092
MaeIII GTNAC 4 cut(s) 255, 379, 781, 1075
MalI GATC 7 cut(s) 476, 560, 602, 625, 735, 902, 971
MboI GATC 7 cut(s) 474, 558, 600, 623, 733, 900, 969
MboII GAAGA 7 cut(s) 46, 392, 610, 727, 841, 1040, 1072
MfeI CAATTG 1 cut(s) 384
MhlI GDGCHC 2 cut(s) 613, 758
MluCI AATT 4 cut(s) 384, 527, 776, 888
MlyI GAGTC 5 cut(s) 159, 188, 331, 712, 734
MmeI TCCRAC 1 cut(s) 1028
Mph1103I ATGCAT 1 cut(s) 422
MslI CAYNNNNRTG 2 cut(s) 768, 962
MspA1I CMGCKG 1 cut(s) 448
MspI CCGG 9 cut(s) 72, 472, 494, 590, 759, 813, 921, 998, 1043
MspR9I CCNGG 3 cut(s) 494, 590, 844
MunI CAATTG 1 cut(s) 384
Mva1269I GAATGC 1 cut(s) 420
MvaI CCWGG 1 cut(s) 844
MvnI CGCG 2 cut(s) 533, 586
MwoI GCNNNNNNNGC 5 cut(s) 268, 530, 694, 994, 1108
NciI CCSGG 2 cut(s) 494, 590
NcoI CCATGG 1 cut(s) 170
NdeII GATC 7 cut(s) 474, 558, 600, 623, 733, 900, 969
NlaIII CATG 5 cut(s) 174, 420, 701, 734, 985
NlaIV GGNNCC 2 cut(s) 925, 1002
NmeAIII GCCGAG 2 cut(s) 247, 809
NmuCI GTSAC 4 cut(s) 255, 379, 781, 1075
NsiI ATGCAT 1 cut(s) 422
OliI CACNNNNGTG 1 cut(s) 962
PaeR7I CTCGAG 1 cut(s) 8
PagI TCATGA 1 cut(s) 730
PcsI WCGNNNNNNNCGW 1 cut(s) 1076
PctI GAATGC 1 cut(s) 420
PfeI GAWTC 1 cut(s) 618
PflFI GACNNNGTC 1 cut(s) 791
PflMI CCANNNNNTGG 2 cut(s) 229, 646
PkrI GCNGC 4 cut(s) 236, 519, 566, 835
PleI GAGTC 5 cut(s) 159, 188, 330, 712, 733
PmaCI CACGTG 2 cut(s) 964, 1087
PmlI CACGTG 2 cut(s) 964, 1087
PpsI GAGTC 5 cut(s) 159, 188, 330, 712, 733
Ppu21I YACGTR 3 cut(s) 964, 1087, 1093
Psp124BI GAGCTC 1 cut(s) 613
Psp6I CCWGG 1 cut(s) 842
PspCI CACGTG 2 cut(s) 964, 1087
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 1 cut(s) 842
PspN4I GGNNCC 2 cut(s) 925, 1002
PspPI GGNCC 3 cut(s) 276, 553, 1131
PsyI GACNNNGTC 1 cut(s) 791
RsaI GTAC 6 cut(s) 333, 488, 956, 992, 1082, 1091
RsaNI GTAC 6 cut(s) 332, 487, 955, 991, 1081, 1090
RseI CAYNNNNRTG 2 cut(s) 768, 962
SacI GAGCTC 1 cut(s) 613
SatI GCNGC 4 cut(s) 235, 518, 565, 834
Sau3AI GATC 7 cut(s) 474, 558, 600, 623, 733, 900, 969
Sau96I GGNCC 3 cut(s) 276, 553, 1131
SchI GAGTC 5 cut(s) 159, 188, 331, 712, 734
ScrFI CCNGG 3 cut(s) 494, 590, 844
SduI GDGCHC 2 cut(s) 613, 758
SexAI ACCWGGT 1 cut(s) 842
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 2 cut(s) 276, 553
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 2 cut(s) 768, 962
SmlI CTYRAG 5 cut(s) 8, 62, 289, 751, 893
SmoI CTYRAG 5 cut(s) 8, 62, 289, 751, 893
Sse9I AATT 4 cut(s) 384, 527, 776, 888
SsiI CCGC 8 cut(s) 260, 448, 518, 533, 833, 995, 1022, 1102
SspMI CTAG 1 cut(s) 744
SstI GAGCTC 1 cut(s) 613
StyD4I CCNGG 3 cut(s) 492, 588, 842
StyI CCWWGG 2 cut(s) 170, 351
TaaI ACNGT 4 cut(s) 379, 644, 682, 1075
TaiI ACGT 6 cut(s) 660, 966, 1038, 1082, 1089, 1095
TaqI TCGA 6 cut(s) 9, 80, 426, 459, 543, 879
TaqII GACCGA 1 cut(s) 425
TasI AATT 4 cut(s) 384, 527, 776, 888
TatI WGTACW 1 cut(s) 954
TauI GCSGC 2 cut(s) 520, 836
TfiI GAWTC 1 cut(s) 618
TscAI CASTG 4 cut(s) 184, 647, 790, 1080
TseFI GTSAC 4 cut(s) 255, 379, 781, 1075
TseI GCWGC 2 cut(s) 234, 564
Tsp45I GTSAC 4 cut(s) 255, 379, 781, 1075
TspDTI ATGAA 4 cut(s) 46, 120, 714, 951
TspGWI ACGGA 3 cut(s) 319, 929, 1130
TspRI CASTG 4 cut(s) 184, 647, 790, 1080
Tth111I GACNNNGTC 1 cut(s) 791
Van91I CCANNNNNTGG 2 cut(s) 229, 646
VpaK11BI GGWCC 2 cut(s) 276, 553
XapI RAATTY 3 cut(s) 527, 776, 888
XcmI CCANNNNNNNNNTGG 2 cut(s) 270, 349
XhoI CTCGAG 1 cut(s) 8
XspI CTAG 1 cut(s) 744
Zsp2I ATGCAT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.