RchiOBHm_Chr5g0037511

(3S,6E)-nerolidol synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0025012
Physical Location & Seq
Forward (+)
32024205 .. 32025712
1508 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1185 bp
ATGGCGTCCATTCTTCAAGCTCACTCTGCTTCCTCAAAGTCTGAGATCGGTGATCTTCACGTCCGTCGTGAAGAAAAACTGAGGGAGTTGAAGGAAACACTCGCAAATGCCGGGGAAGATCAACTGCAGCAATTGGTTATGGTTGATGCCATGCAACGCCTCGGCGTCGCTTATCTCTTCCAAAATGAAATTTATGCAATCTTAGAGAAGCAATACACCAGGTCATCAGCTTATAATGGTGATGATGATGGCAATCATCAACTTCTTGAGGCTTCCCTTCGTTTTCGACTATTGAGACAACAAGGTTTCCCTGTAACTCCAGGTAGATATTGTTCTTACCACTTTGTCTTAACTCAAATTCAACAAGGAAAGTTGATGATAATATGTACTGACTTAATGAGGATGACAGATGCAGAGGCGTGTAACAAGATCAAGAACAATGAGAAGCTCAAATCCGGACTAATAGCTAGTGATGAAGACATAAATGGATTAGTGGAATTATGTGAAGCATCTCACTTGAGTTTCCAAGGAGAAGAAATGCTTGACGAAGCAGGAAAGCTCAGCCGCCAGCTTCTCACTGCCTGGGTCTCTAACAATCACAATGATCATCGCGTAACACTTGTCGAGCGCACTTTGGAGCATCCCTATCACAAGAGCCTGACAAGATTCATGGCCAAGAAGTTTTTGAATAATTTTCAAGGCCAAGAAAAATGGGTTTGTTCTTTACAAGAGCTAGCAAACCTAGAGTTCAACATGGTTGAATCCATAATCCAAAATGAAATCCTACTAGTATCTGGATGGTGGAAAGAACTAGGTTTGACCAGAGAGTTGAAGTTTGTGAGAGACCAGCCAATTAAATGGTACACATGGCCGATGGCATGCCTCGTTGATCCACGATTATCAGAGGAAAGGATTGAACTCACAAAAGCAGTCTCTCTTGTTTACATTATAGATGATATTTTTGATGTTCGTGGAACACTAGATGAACTCGTTCTCTTTACAGCAGCAGTTGCGAGATGGGATACTGCTACAAATGATGAGCTACCGAATTACATGAAAACATGTTTCAAAGCTCTTAATGACATTACCAATAATATCAGCCAGTGTGTCCACGAAAAGCACGGATGGAACCCTATTGAAATCCTAAGAGAATCGGTACATATACATATATCAAGTAATATGTAA

Protein Analysis

394

Amino Acids

45.39

Weight (kDa)

5.52

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 15 - 107 1.2e-17 Terpene synthase, N-terminal domain
Terpene_synth PF01397 144 - 207 1.5e-07 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 269 - 392 7.4e-36 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 309 - 386 8.2e-14 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025012)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0037511
rosa_multiflora Rmu_sc0002521.1_g000039
rosa_samantha Rh5CG289800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 234
AccII CGCG 1 cut(s) 612
AccIII TCCGGA 1 cut(s) 455
AciI CCGC 1 cut(s) 565
AclWI GGATC 1 cut(s) 884
AcoI YGGCCR 2 cut(s) 672, 869
AcsI RAATTY 2 cut(s) 189, 357
AcyI GRCGYC 2 cut(s) 5, 165
AfaI GTAC 3 cut(s) 388, 863, 1158
AflIII ACRYGT 1 cut(s) 1061
AhlI ACTAGT 1 cut(s) 787
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 3 cut(s) 218, 319, 581
AluBI AGCT 9 cut(s) 20, 230, 448, 467, 559, 571, 733, 1042, 1073
AluI AGCT 9 cut(s) 20, 230, 448, 467, 559, 571, 733, 1042, 1073
Alw26I GTCTC 4 cut(s) 289, 592, 837, 937
AlwI GGATC 1 cut(s) 884
Aor13HI TCCGGA 1 cut(s) 455
AoxI GGCC 3 cut(s) 672, 700, 869
ApeKI GCWGC 2 cut(s) 127, 1004
ApoI RAATTY 2 cut(s) 189, 357
Asp700I GAANNNNTTC 1 cut(s) 990
AspLEI GCGC 1 cut(s) 630
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 2 cut(s) 62, 251
AsuNHI GCTAGC 1 cut(s) 733
BalI TGGCCA 1 cut(s) 674
BbsI GAAGAC 1 cut(s) 483
BbvI GCAGC 2 cut(s) 139, 1016
BccI CCATC 5 cut(s) 242, 792, 868, 1011, 1119
BciT130I CCWGG 3 cut(s) 220, 321, 583
BciVI GTATCC 1 cut(s) 1015
BclI TGATCA 1 cut(s) 604
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 4 cut(s) 289, 592, 837, 937
BcuI ACTAGT 1 cut(s) 787
BfaI CTAG 6 cut(s) 468, 734, 743, 788, 812, 980
BfmI CTRYAG 1 cut(s) 125
BfuI GTATCC 1 cut(s) 1015
BisI GCNGC 3 cut(s) 128, 565, 1005
BlpI GCTNAGC 1 cut(s) 560
BlsI GCNGC 3 cut(s) 129, 566, 1006
Bme1390I CCNGG 4 cut(s) 112, 220, 321, 583
BmgBI CACGTC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 1130
BmrFI CCNGG 4 cut(s) 112, 220, 321, 583
BmsI GCATC 4 cut(s) 136, 400, 518, 649
BmtI GCTAGC 1 cut(s) 737
BpiI GAAGAC 1 cut(s) 483
BpmI CTGGAG 1 cut(s) 303
Bpu1102I GCTNAGC 1 cut(s) 560
BpuEI CTTGAG 2 cut(s) 287, 538
BpuMI CCSGG 1 cut(s) 112
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 2 cut(s) 5, 165
BsaI GGTCTC 2 cut(s) 592, 837
BsaJI CCNNGG 4 cut(s) 111, 160, 526, 582
BsaWI WCCGGW 1 cut(s) 455
Bse1I ACTGG 1 cut(s) 1102
Bse8I GATNNNNATC 1 cut(s) 252
BseAI TCCGGA 1 cut(s) 455
BseBI CCWGG 3 cut(s) 220, 321, 583
BseDI CCNNGG 4 cut(s) 111, 160, 526, 582
BseGI GGATG 4 cut(s) 408, 640, 803, 1130
BseJI GATNNNNATC 1 cut(s) 252
BseMII CTCAG 3 cut(s) 33, 71, 574
BseNI ACTGG 1 cut(s) 1102
BseXI GCAGC 2 cut(s) 139, 1016
Bsh1236I CGCG 1 cut(s) 612
BshFI GGCC 3 cut(s) 674, 702, 871
BsiSI CCGG 2 cut(s) 111, 456
BsmAI GTCTC 4 cut(s) 289, 592, 837, 937
BsnI GGCC 3 cut(s) 674, 702, 871
Bso31I GGTCTC 2 cut(s) 592, 837
Bsp13I TCCGGA 1 cut(s) 455
Bsp143I GATC 6 cut(s) 45, 52, 118, 429, 604, 889
Bsp1720I GCTNAGC 1 cut(s) 560
BspACI CCGC 1 cut(s) 565
BspANI GGCC 3 cut(s) 674, 702, 871
BspCNI CTCAG 3 cut(s) 34, 72, 573
BspEI TCCGGA 1 cut(s) 455
BspFNI CGCG 1 cut(s) 612
BspLI GGNNCC 1 cut(s) 1130
BspMAI CTGCAG 1 cut(s) 129
BspOI GCTAGC 1 cut(s) 737
BspPI GGATC 1 cut(s) 884
BspTNI GGTCTC 2 cut(s) 592, 837
BsrI ACTGG 1 cut(s) 1102
BssECI CCNNGG 4 cut(s) 111, 160, 526, 582
BssMI GATC 6 cut(s) 45, 52, 118, 429, 604, 889
BssNI GRCGYC 2 cut(s) 5, 165
BssT1I CCWWGG 1 cut(s) 526
Bst2UI CCWGG 3 cut(s) 220, 321, 583
Bst6I CTCTTC 1 cut(s) 182
BstACI GRCGYC 2 cut(s) 5, 165
BstAPI GCANNNNNTGC 1 cut(s) 1010
BstC8I GCNNGC 3 cut(s) 569, 735, 880
BstDEI CTNAG 5 cut(s) 42, 80, 202, 560, 1145
BstF5I GGATG 4 cut(s) 408, 640, 803, 1130
BstFNI CGCG 1 cut(s) 612
BstHHI GCGC 1 cut(s) 630
BstKTI GATC 6 cut(s) 48, 55, 121, 432, 607, 892
BstMAI GTCTC 4 cut(s) 289, 592, 837, 937
BstMBI GATC 6 cut(s) 45, 52, 118, 429, 604, 889
BstMWI GCNNNNNNNGC 2 cut(s) 26, 1010
BstNI CCWGG 3 cut(s) 220, 321, 583
BstNSI RCATGY 2 cut(s) 882, 1065
BstSCI CCNGG 4 cut(s) 110, 218, 319, 581
BstSFI CTRYAG 1 cut(s) 125
BstUI CGCG 1 cut(s) 612
BstV1I GCAGC 2 cut(s) 139, 1016
BstV2I GAAGAC 1 cut(s) 483
BstXI CCANNNNNNTGG 1 cut(s) 858
BsuI GTATCC 1 cut(s) 1015
BsuRI GGCC 3 cut(s) 674, 702, 871
BtgZI GCGATG 1 cut(s) 593
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 4 cut(s) 408, 640, 803, 1130
BtsI GCAGTG 1 cut(s) 576
BtsIMutI CAGTG 2 cut(s) 576, 1109
Cac8I GCNNGC 3 cut(s) 569, 735, 880
CfoI GCGC 1 cut(s) 630
CseI GACGC 1 cut(s) 154
CsiI ACCWGGT 1 cut(s) 218
Csp6I GTAC 3 cut(s) 387, 862, 1157
CviAII CATG 7 cut(s) 151, 670, 754, 867, 879, 1054, 1062
CviQI GTAC 3 cut(s) 387, 862, 1157
DdeI CTNAG 5 cut(s) 42, 80, 202, 560, 1145
DpnI GATC 6 cut(s) 47, 54, 120, 431, 606, 891
DpnII GATC 6 cut(s) 45, 52, 118, 429, 604, 889
EaeI YGGCCR 2 cut(s) 672, 869
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 526
Eco31I GGTCTC 2 cut(s) 592, 837
EcoRII CCWGG 3 cut(s) 218, 319, 581
EcoT14I CCWWGG 1 cut(s) 526
ErhI CCWWGG 1 cut(s) 526
FaeI CATG 7 cut(s) 154, 673, 757, 870, 882, 1057, 1065
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FatI CATG 7 cut(s) 150, 669, 753, 866, 878, 1053, 1061
FbaI TGATCA 1 cut(s) 604
Fnu4HI GCNGC 3 cut(s) 128, 565, 1005
FokI GGATG 4 cut(s) 415, 627, 810, 1137
Fsp4HI GCNGC 3 cut(s) 128, 565, 1005
FspBI CTAG 6 cut(s) 468, 734, 743, 788, 812, 980
GlaI GCGC 1 cut(s) 629
GluI GCNGC 3 cut(s) 128, 565, 1005
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 3 cut(s) 674, 702, 871
HapII CCGG 2 cut(s) 111, 456
HgaI GACGC 1 cut(s) 154
HhaI GCGC 1 cut(s) 630
Hin1I GRCGYC 2 cut(s) 5, 165
Hin1II CATG 7 cut(s) 154, 673, 757, 870, 882, 1057, 1065
Hin6I GCGC 1 cut(s) 628
HinP1I GCGC 1 cut(s) 628
HinfI GANTC 3 cut(s) 666, 761, 1151
HpaII CCGG 2 cut(s) 111, 456
HphI GGTGA 2 cut(s) 62, 251
Hpy166II GTNNAC 3 cut(s) 864, 943, 1111
Hpy188I TCNGA 2 cut(s) 43, 904
Hpy188III TCNNGA 5 cut(s) 68, 266, 433, 456, 795
Hpy8I GTNNAC 3 cut(s) 864, 943, 1111
Hpy99I CGWCG 2 cut(s) 69, 170
HpyAV CCTTC 2 cut(s) 85, 287
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 4 cut(s) 127, 154, 197, 413
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 1010
HpyF3I CTNAG 5 cut(s) 42, 80, 202, 560, 1145
HpySE526I ACGT 1 cut(s) 60
Hsp92I GRCGYC 2 cut(s) 5, 165
Hsp92II CATG 7 cut(s) 154, 673, 757, 870, 882, 1057, 1065
HspAI GCGC 1 cut(s) 628
Kpn2I TCCGGA 1 cut(s) 455
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 6 cut(s) 45, 52, 118, 429, 604, 889
LmnI GCTCC 1 cut(s) 637
Lsp1109I GCAGC 2 cut(s) 139, 1016
LweI GCATC 4 cut(s) 136, 400, 518, 649
MabI ACCWGGT 1 cut(s) 218
MaeI CTAG 6 cut(s) 468, 734, 743, 788, 812, 980
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 3 cut(s) 313, 422, 613
MalI GATC 6 cut(s) 47, 54, 120, 431, 606, 891
MboI GATC 6 cut(s) 45, 52, 118, 429, 604, 889
MboII GAAGA 7 cut(s) 5, 47, 83, 128, 169, 488, 545
MfeI CAATTG 1 cut(s) 131
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 7 cut(s) 131, 189, 357, 497, 691, 852, 1048
MluNI TGGCCA 1 cut(s) 674
MnlI CCTC 8 cut(s) 43, 75, 170, 262, 393, 409, 893, 898
Mox20I TGGCCA 1 cut(s) 674
MroI TCCGGA 1 cut(s) 455
MroXI GAANNNNTTC 1 cut(s) 990
MscI TGGCCA 1 cut(s) 674
MseI TTAA 4 cut(s) 350, 395, 855, 1077
Msp20I TGGCCA 1 cut(s) 674
MspI CCGG 2 cut(s) 111, 456
MspR9I CCNGG 4 cut(s) 112, 220, 321, 583
MunI CAATTG 1 cut(s) 131
MvaI CCWGG 3 cut(s) 220, 321, 583
MvnI CGCG 1 cut(s) 612
MwoI GCNNNNNNNGC 2 cut(s) 26, 1010
NciI CCSGG 1 cut(s) 112
NdeII GATC 6 cut(s) 45, 52, 118, 429, 604, 889
NheI GCTAGC 1 cut(s) 733
NlaIII CATG 7 cut(s) 154, 673, 757, 870, 882, 1057, 1065
NlaIV GGNNCC 1 cut(s) 1130
NmeAIII GCCGAG 1 cut(s) 141
NspI RCATGY 2 cut(s) 882, 1065
PaeI GCATGC 1 cut(s) 882
PciI ACATGT 1 cut(s) 1061
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 3 cut(s) 666, 761, 1151
PkrI GCNGC 3 cut(s) 129, 566, 1006
PscI ACATGT 1 cut(s) 1061
PsiI TTATAA 1 cut(s) 234
Psp6I CCWGG 3 cut(s) 218, 319, 581
PspGI CCWGG 3 cut(s) 218, 319, 581
PspN4I GGNNCC 1 cut(s) 1130
PsrI GAACNNNNNNTAC 2 cut(s) 316, 348
PstI CTGCAG 1 cut(s) 129
RsaI GTAC 3 cut(s) 388, 863, 1158
RsaNI GTAC 3 cut(s) 387, 862, 1157
SaqAI TTAA 4 cut(s) 350, 395, 855, 1077
SatI GCNGC 3 cut(s) 128, 565, 1005
Sau3AI GATC 6 cut(s) 45, 52, 118, 429, 604, 889
ScrFI CCNGG 4 cut(s) 112, 220, 321, 583
SexAI ACCWGGT 1 cut(s) 218
SfaNI GCATC 4 cut(s) 136, 400, 518, 649
SfcI CTRYAG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 266, 517
SmoI CTYRAG 2 cut(s) 266, 517
SpeI ACTAGT 1 cut(s) 787
SphI GCATGC 1 cut(s) 882
Sse9I AATT 7 cut(s) 131, 189, 357, 497, 691, 852, 1048
SsiI CCGC 1 cut(s) 565
SspMI CTAG 6 cut(s) 468, 734, 743, 788, 812, 980
StyD4I CCNGG 4 cut(s) 110, 218, 319, 581
StyI CCWWGG 1 cut(s) 526
TaiI ACGT 1 cut(s) 63
TaqI TCGA 2 cut(s) 286, 624
TasI AATT 7 cut(s) 131, 189, 357, 497, 691, 852, 1048
TatI WGTACW 1 cut(s) 386
TauI GCSGC 1 cut(s) 567
TfiI GAWTC 3 cut(s) 666, 761, 1151
Tru1I TTAA 4 cut(s) 350, 395, 855, 1077
Tru9I TTAA 4 cut(s) 350, 395, 855, 1077
TscAI CASTG 2 cut(s) 583, 1109
TseI GCWGC 2 cut(s) 127, 1004
TspDTI ATGAA 6 cut(s) 201, 489, 658, 792, 999, 1070
TspGWI ACGGA 2 cut(s) 53, 1137
TspRI CASTG 2 cut(s) 583, 1109
XapI RAATTY 2 cut(s) 189, 357
XceI RCATGY 2 cut(s) 882, 1065
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 6 cut(s) 468, 734, 743, 788, 812, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.